Restriction map for
pF1KB1569
(Circular) (Six-base) MAPSORT of: pF1KB1569.seq Check: 2480 from: 1 to: 6062
pF1KB1569 6062 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1010 1984 1010
Size: 974 5088
AccI GT'mk_AC
Cuts at: 698 927 3100 698
Size: 229 2173 3660
Fragments arranged by size:
3660 2173 229
Acc65I G'GTAC_C
Cuts at: 753 3078 753
Size: 2325 3737
AclI AA'CG_TT
Cuts at: 5902 5902
Size: 6062
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 2791 3887 4319 4672 5334 613
Size: 2178 1096 432 353 662 1341
Fragments arranged by size:
2178 1341 1096 662 432 353
AflIII A'CryG_T
Cuts at: 3414 5214 5495 3414
Size: 1800 281 3981
Fragments arranged by size:
3981 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 768 768
Size: 6062
AleI CACnn'nnGTG
Cuts at: 258 1285 2908 258
Size: 1027 1623 3412
Fragments arranged by size:
3412 1623 1027
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 391 423 391
Size: 32 6030
AlwNI CAG_nnn'CTG
Cuts at: 774 1651 2437 3367 4805 774
Size: 877 786 930 1438 2031
Fragments arranged by size:
2031 1438 930 877 786
ApaLI G'TGCA_C
Cuts at: 4900 4900
Size: 6062
ApoI r'AATT_y
Cuts at: 508 1999 3066 5415 5671 508
Size: 1491 1067 2349 256 899
Fragments arranged by size:
2349 1491 1067 899 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6062
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6062
AvaI C'yCGr_G
Cuts at: 3082 3082
Size: 6062
BamHI G'GATC_C
Cuts at: 3087 3087
Size: 6062
BanI G'GyrC_C
Cuts at: 753 3078 3492 3740 3775 753
Size: 2325 414 248 35 3040
Fragments arranged by size:
3040 2325 414 248 35
BanII G_rGCy'C
Cuts at: 404 1366 1641 3076 4106 404
Size: 962 275 1435 1030 2360
Fragments arranged by size:
2360 1435 1030 962 275
BbeI G_GCGC'C
Cuts at: 3496 3744 3496
Size: 248 5814
BbsI GAAGACnn'nnnn_
Cuts at: 1922 1922
Size: 6062
BciVI GTATCCnnnnn_n'
Cuts at: 3960 5016 6062 3960
Size: 1056 1046 3960
Fragments arranged by size:
3960 1056 1046
BclI T'GATC_A
Cuts at: 495 2366 3582 495
Size: 1871 1216 2975
Fragments arranged by size:
2975 1871 1216
BglII A'GATC_T
Cuts at: 3577 5614 3577
Size: 2037 4025
BlpI GC'TnA_GC
Cuts at: 1360 3174 1360
Size: 1814 4248
Bme1580I G_kGCm'C
Cuts at: 715 979 3687 3780 4904 715
Size: 264 2708 93 1124 1873
Fragments arranged by size:
2708 1873 1124 264 93
BmgBI CAC'GTC
Cuts at: 2513 2513
Size: 6062
BmrI ACTGGGnnnn_n'
Cuts at: 372 762 2250 2592 3040 3274 3355 3690 5834
Size: 390 1488 342 448 234 81 335 2144
Cuts at: 5834 372
Size: 600
Fragments arranged by size:
2144 1488 600 448 390 342 335 234
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1380 4429 1380
Size: 3049 3013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1161 1437 2440 1161
Size: 276 1003 4783
Fragments arranged by size:
4783 1003 276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1588 3238 4117 4831 5129 388
Size: 1200 1650 879 714 298 1321
Fragments arranged by size:
1650 1321 1200 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1114 1888 2336 2568 5766 1114
Size: 774 448 232 3198 1410
Fragments arranged by size:
3198 1410 774 448 232
BsaAI yAC'GTr
Cuts at: 1623 2242 3415 1623
Size: 619 1173 4270
Fragments arranged by size:
4270 1173 619
BsaBI GATnn'nnATC
Cuts at: 6 494 2467 3576 5613 6
Size: 488 1973 1109 2037 455
Fragments arranged by size:
2037 1973 1109 488 455
BsaHI Gr'CG_yC
Cuts at: 3493 3741 4443 5939 3493
Size: 248 702 1496 3616
Fragments arranged by size:
3616 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2753 3251 3772 4450 4861 5008 5282 2753
Size: 498 521 678 411 147 274 3533
Fragments arranged by size:
3533 678 521 498 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 391 421 1065 1095 1690 1720 1794 1824 2545
Size: 30 644 30 595 30 74 30 721
Cuts at: 2545 2575 391
Size: 30 3878
Fragments arranged by size:
3878 721 644 595 74 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 121 1762 2112 2468 121
Size: 1641 350 356 3715
Fragments arranged by size:
3715 1641 356 350
BseYI C'CCAG_C
Cuts at: 231 2655 3487 4245 4910 5318 231
Size: 2424 832 758 665 408 975
Fragments arranged by size:
2424 975 832 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2034 2807 2034
Size: 773 5289
BsiEI CG_ry'CG
Cuts at: 104 3604 3650 4880 104
Size: 3500 46 1230 1286
Fragments arranged by size:
3500 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 404 1366 3076 3854 4044 4904 404
Size: 962 1710 778 190 860 1562
Fragments arranged by size:
1710 1562 962 860 778 190
BsmI GAATG_Cn'
Cuts at: 431 431
Size: 6062
Bsp1286I G_dGCh'C
Cuts at: 404 715 979 1366 1641 3076 3687 3780 3854
Size: 311 264 387 275 1435 611 93 74
Cuts at: 3854 4044 4106 4904 404
Size: 190 62 798 1562
Fragments arranged by size:
1562 1435 798 611 387 311 275 264
190 93 74 62
BspEI T'CCGG_A
Cuts at: 2753 2753
Size: 6062
BspHI T'CATG_A
Cuts at: 1736 1935 4494 1736
Size: 199 2559 3304
Fragments arranged by size:
3304 2559 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1010 1984 3112 3326 3628 4009 1010
Size: 974 1128 214 302 381 3063
Fragments arranged by size:
3063 1128 974 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4354 4408 5892 6057 50
Size: 4304 54 1484 165 55
Fragments arranged by size:
4304 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 542 891 1866 2832 3974 327
Size: 215 349 975 966 1142 2415
Fragments arranged by size:
2415 1142 975 966 349 215
BsrFI r'CCGG_y
Cuts at: 4060 4241 4060
Size: 181 5881
BssSI C'ACGA_G
Cuts at: 4333 5041 4333
Size: 708 5354
BstAPI GCAn_nnn'nTGC
Cuts at: 3439 3439
Size: 6062
BstBI TT'CG_AA
Cuts at: 4423 4423
Size: 6062
BstXI CCAn_nnnn'nTGG
Cuts at: 1953 2474 1953
Size: 521 5541
BstYI r'GATC_y
Cuts at: 1 239 573 819 2462 3087 3554 3577 3912
Size: 238 334 246 1643 625 467 23 335
Cuts at: 3912 4158 4464 4476 4562 5614 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1643 1052 625 467 449 335 334 306
246 246 238 86 23 12
BstZ17I GTA'TAC
Cuts at: 699 699
Size: 6062
BtgI C'CryG_G
Cuts at: 107 758 1064 1283 2645 2978 3053 107
Size: 651 306 219 1362 333 75 3116
Fragments arranged by size:
3116 1362 651 333 306 219 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2537 4012 4193 4356 2537
Size: 1475 181 163 4243
Fragments arranged by size:
4243 1475 181 163
BtsI GCAGTG_nn'
Cuts at: 2022 2886 3305 2022
Size: 864 419 4779
Fragments arranged by size:
4779 864 419
DraI TTT'AAA
Cuts at: 1871 3061 1871
Size: 1190 4872
DrdI GACnn_nn'nnGTC
Cuts at: 1519 3768 5112 1519
Size: 2249 1344 2469
Fragments arranged by size:
2469 2249 1344
EaeI y'GGCC_r
Cuts at: 2057 3647 3821 4212 4239 5584 2057
Size: 1590 174 391 27 1345 2535
Fragments arranged by size:
2535 1590 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3647 3647
Size: 6062
EarI CTCTTCn'nnn_
Cuts at: 260 4085 4295 260
Size: 3825 210 2027
Fragments arranged by size:
3825 2027 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4270 5012 5158 5872 4270
Size: 742 146 714 4460
Fragments arranged by size:
4460 742 714 146
EcoICRI GAG'CTC
Cuts at: 402 1364 3074 402
Size: 962 1710 3390
Fragments arranged by size:
3390 1710 962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1161 1437 2440 2791 3887 4319 4672 5334
Size: 548 276 1003 351 1096 432 353 662
Cuts at: 5334 613
Size: 1341
Fragments arranged by size:
1341 1096 1003 662 548 432 353 351
276
EcoNI CCTnn'n_nnAGG
Cuts at: 778 778
Size: 6062
EcoO109I rG'GnC_Cy
Cuts at: 366 622 3201 366
Size: 256 2579 3227
Fragments arranged by size:
3227 2579 256
EcoRI G'AATT_C
Cuts at: 3066 3066
Size: 6062
EcoRV GAT'ATC
Cuts at: 2835 2835
Size: 6062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2916 4081 2916
Size: 1165 4897
FspI TGC'GCA
Cuts at: 3843 3843
Size: 6062
HaeII r_GCGC'y
Cuts at: 3496 3744 4974 5745 3496
Size: 248 1230 771 3813
Fragments arranged by size:
3813 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 754 786 1045 1077 1435 1467 1690
Size: 32 491 32 259 32 358 32 223
Cuts at: 1690 1722 2447 2479 2545 2577 2580 2612 3904
Size: 32 725 32 66 32 3 32 1292
Cuts at: 3904 3934 3936 3966 231
Size: 30 2 30 2327
Fragments arranged by size:
2327 1292 725 491 358 259 223 66
32 32 32 32 32 32 32 32
30 30 3 2
HincII GTy'rAC
Cuts at: 630 801 928 1899 3101 630
Size: 171 127 971 1202 3591
Fragments arranged by size:
3591 1202 971 171 127
HindIII A'AGCT_T
Cuts at: 920 2746 920
Size: 1826 4236
KasI G'GCGC_C
Cuts at: 3492 3740 3492
Size: 248 5814
KpnI G_GTAC'C
Cuts at: 757 3082 757
Size: 2325 3737
MfeI C'AATT_G
Cuts at: 345 345
Size: 6062
MluI A'CGCG_T
Cuts at: 5495 5495
Size: 6062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1968 4821 5005 1968
Size: 2853 184 3025
Fragments arranged by size:
3025 2853 184
MscI TGG'CCA
Cuts at: 2059 3823 2059
Size: 1764 4298
MslI CAynn'nnrTG
Cuts at: 258 568 1285 1496 1910 2908 4051 4178 258
Size: 310 717 211 414 998 1143 127 2142
Fragments arranged by size:
2142 1143 998 717 414 310 211 127
MspA1I CmG'CkG
Cuts at: 143 550 1651 1753 2659 2673 3173 3487 3847
Size: 407 1101 102 906 14 500 314 360
Cuts at: 3847 4631 4876 143
Size: 784 245 1329
Fragments arranged by size:
1329 1101 906 784 500 407 360 314
245 102 14
NaeI GCC'GGC
Cuts at: 4243 4243
Size: 6062
NarI GG'CG_CC
Cuts at: 3493 3741 3493
Size: 248 5814
NcoI C'CATG_G
Cuts at: 107 758 1064 2645 107
Size: 651 306 1581 3524
Fragments arranged by size:
3524 1581 651 306
NdeI CA'TA_TG
Cuts at: 1192 1911 1192
Size: 719 5343
NgoMIV G'CCGG_C
Cuts at: 4241 4241
Size: 6062
NspI r_CATG'y
Cuts at: 3115 3615 5218 3115
Size: 500 1603 3959
Fragments arranged by size:
3959 1603 500
PciI A'CATG_T
Cuts at: 5214 5214
Size: 6062
PflMI CCAn_nnn'nTGG
Cuts at: 256 774 256
Size: 518 5544
PfoI T'CCnGG_A
Cuts at: 1133 2417 1133
Size: 1284 4778
PmeI GTTT'AAAC
Cuts at: 3061 3061
Size: 6062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 391 423 4508 4540 391
Size: 32 4085 32 1913
Fragments arranged by size:
4085 1913 32 32
PpuMI rG'GwC_Cy
Cuts at: 366 622 366
Size: 256 5806
PshAI GACnn'nnGTC
Cuts at: 335 335
Size: 6062
PstI C_TGCA'G
Cuts at: 841 1021 3109 3794 841
Size: 180 2088 685 3109
Fragments arranged by size:
3109 2088 685 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6062
PvuII CAG'CTG
Cuts at: 143 550 2659 2673 3487 3847 143
Size: 407 2109 14 814 360 2358
Fragments arranged by size:
2358 2109 814 407 360 14
RsrII CG'GwC_CG
Cuts at: 4257 4257
Size: 6062
SacI G_AGCT'C
Cuts at: 404 1366 3076 404
Size: 962 1710 3390
Fragments arranged by size:
3390 1710 962
SalI G'TCGA_C
Cuts at: 926 3099 926
Size: 2173 3889
SanDI GG'GwC_CC
Cuts at: 366 366
Size: 6062
SapI GCTCTTCn'nnn_
Cuts at: 4085 4295 4085
Size: 210 5852
SbfI CC_TGCA'GG
Cuts at: 3109 3109
Size: 6062
ScaI AGT'ACT
Cuts at: 5579 5579
Size: 6062
SexAI A'CCwGG_T
Cuts at: 311 930 311
Size: 619 5443
SfcI C'TryA_G
Cuts at: 33 837 1017 2492 2615 3105 3790 4758 4949
Size: 804 180 1475 123 490 685 968 191
Cuts at: 4949 33
Size: 1146
Fragments arranged by size:
1475 1146 968 804 685 490 191 180
123
SfoI GGC'GCC
Cuts at: 3494 3742 3494
Size: 248 5814
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6062
SmaI CCC'GGG
Cuts at: 3084 3084
Size: 6062
SmlI C'TyrA_G
Cuts at: 403 1603 3217 4132 4846 5108 403
Size: 1200 1614 915 714 262 1357
Fragments arranged by size:
1614 1357 1200 915 714 262
SnaBI TAC'GTA
Cuts at: 1623 2242 1623
Size: 619 5443
SpeI A'CTAG_T
Cuts at: 67 1690 67
Size: 1623 4439
SphI G_CATG'C
Cuts at: 3115 3615 3115
Size: 500 5562
StyI C'CwwG_G
Cuts at: 107 680 758 826 1064 1946 2225 2645 3196
Size: 573 78 68 238 882 279 420 551
Cuts at: 3196 3550 107
Size: 354 2619
Fragments arranged by size:
2619 882 573 551 420 354 279 238
78 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 248 312 1196 3645 3778 4237 4446 248
Size: 64 884 2449 133 459 209 1864
Fragments arranged by size:
2449 1864 884 459 209 133 64
TatI w'GTAC_w
Cuts at: 473 833 841 2915 5577 473
Size: 360 8 2074 2662 958
Fragments arranged by size:
2662 2074 958 360 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1794 1826 5579 5611 1794
Size: 32 3753 32 2245
Fragments arranged by size:
3753 2245 32 32
Tth111I GACn'n_nGTC
Cuts at: 3859 3859
Size: 6062
XbaI T'CTAG_A
Cuts at: 3021 3093 3021
Size: 72 5990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 257 3557 257
Size: 3300 2762
XmaI C'CCGG_G
Cuts at: 3082 3082
Size: 6062
XmnI GAAnn'nnTTC
Cuts at: 173 173
Size: 6062
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PmlI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI