Restriction map for
pF1KB1499
(Circular) (Six-base) MAPSORT of: pF1KB1499.seq Check: 1583 from: 1 to: 7076
pF1KB1499 7076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3103 3103
Size: 7076
AccI GT'mk_AC
Cuts at: 1112 2770 4114 1112
Size: 1658 1344 4074
Fragments arranged by size:
4074 1658 1344
Acc65I G'GTAC_C
Cuts at: 2526 2958 3830 4092 2526
Size: 432 872 262 5510
Fragments arranged by size:
5510 872 432 262
AclI AA'CG_TT
Cuts at: 6916 6916
Size: 7076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2205 2831 4901 5333 6080 6348 2205
Size: 626 2070 432 747 268 2933
Fragments arranged by size:
2933 2070 747 626 432 268
AfeI AGC'GCT
Cuts at: 1156 1156
Size: 7076
AflIII A'CryG_T
Cuts at: 3172 3533 4428 5532 6509 3172
Size: 361 895 1104 977 3739
Fragments arranged by size:
3739 1104 977 895 361
AgeI A'CCGG_T
Cuts at: 5464 5464
Size: 7076
AleI CACnn'nnGTG
Cuts at: 1513 1513
Size: 7076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3001 3001
Size: 7076
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 7044
AlwNI CAG_nnn'CTG
Cuts at: 156 231 561 1492 1651 3269 4381 5948 156
Size: 75 330 931 159 1618 1112 1567 1284
Fragments arranged by size:
1618 1567 1284 1112 931 330 159 75
ApaI G_GGCC'C
Cuts at: 117 907 1090 1262 1854 1885 117
Size: 790 183 172 592 31 5308
Fragments arranged by size:
5308 790 592 183 172 31
ApaLI G'TGCA_C
Cuts at: 938 3127 5846 938
Size: 2189 2719 2168
Fragments arranged by size:
2719 2189 2168
ApoI r'AATT_y
Cuts at: 2544 2633 4062 4080 6429 6685 2544
Size: 89 1429 18 2349 256 2935
Fragments arranged by size:
2935 2349 1429 256 89 18
AseI AT'TA_AT
Cuts at: 20 3512 20
Size: 3492 3584
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7076
AvaI C'yCGr_G
Cuts at: 317 608 872 1023 1095 2367 3039 4096 317
Size: 291 264 151 72 1272 672 1057 3297
Fragments arranged by size:
3297 1272 1057 672 291 264 151 72
AvrII C'CTAG_G
Cuts at: 1537 2874 1537
Size: 1337 5739
BamHI G'GATC_C
Cuts at: 2647 4101 2647
Size: 1454 5622
BanI G'GyrC_C
Cuts at: 461 564 958 2526 2958 3187 3283 3790 3830
Size: 103 394 1568 432 229 96 507 40
Cuts at: 3830 4092 4506 4754 4789 461
Size: 262 414 248 35 2748
Fragments arranged by size:
2748 1568 507 432 414 394 262 248
229 103 96 40 35
BanII G_rGCy'C
Cuts at: 117 218 907 1090 1182 1262 1283 1726 1789
Size: 101 689 183 92 80 21 443 63
Cuts at: 1789 1799 1854 1885 2512 2689 2782 3040 4090
Size: 10 55 31 627 177 93 258 1050
Cuts at: 4090 5120 117
Size: 1030 2073
Fragments arranged by size:
2073 1050 1030 689 627 443 258 183
177 101 93 92 80 63 55 31
21 10
BbeI G_GCGC'C
Cuts at: 465 4510 4758 465
Size: 4045 248 2783
Fragments arranged by size:
4045 2783 248
BbsI GAAGACnn'nnnn_
Cuts at: 885 2379 2511 3151 885
Size: 1494 132 640 4810
Fragments arranged by size:
4810 1494 640 132
BbvCI CC'TCA_GC
Cuts at: 635 1393 635
Size: 758 6318
BciVI GTATCCnnnnn_n'
Cuts at: 770 2374 2697 4974 5735 7076 770
Size: 1604 323 2277 761 1341 770
Fragments arranged by size:
2277 1604 1341 770 761 323
BclI T'GATC_A
Cuts at: 3210 4596 3210
Size: 1386 5690
BglI GCCn_nnn'nGGC
Cuts at: 112 646 967 1074 3467 3799 112
Size: 534 321 107 2393 332 3389
Fragments arranged by size:
3389 2393 534 332 321 107
BglII A'GATC_T
Cuts at: 1992 4591 6628 1992
Size: 2599 2037 2440
Fragments arranged by size:
2599 2440 2037
BlpI GC'TnA_GC
Cuts at: 663 1450 2264 4188 663
Size: 787 814 1924 3551
Fragments arranged by size:
3551 1924 814 787
Bme1580I G_kGCm'C
Cuts at: 117 184 569 907 942 1090 1262 1854 1885
Size: 67 385 338 35 148 172 592 31
Cuts at: 1885 3131 3742 3795 4701 4794 5850 117
Size: 1246 611 53 906 93 1056 1343
Fragments arranged by size:
1343 1246 1056 906 611 592 385 338
172 148 93 67 53 35 31
BmgBI CAC'GTC
Cuts at: 1193 1193
Size: 7076
BmrI ACTGGGnnnn_n'
Cuts at: 765 1258 1445 1880 2240 2612 2785 4053 4288
Size: 493 187 435 360 372 173 1268 235
Cuts at: 4288 4369 4704 6848 765
Size: 81 335 2144 993
Fragments arranged by size:
2144 1268 993 493 435 372 360 335
235 187 173 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2949 3147 3795 5443 2949
Size: 198 648 1648 4582
Fragments arranged by size:
4582 1648 648 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1309 1781 1882 2256 2262 2534 2755 2958
Size: 12 472 101 374 6 272 221 203
Cuts at: 2958 3040 1297
Size: 82 5333
Fragments arranged by size:
5333 472 374 272 221 203 101 82
12 6
Bpu10I CC'TnA_GC
Cuts at: 635 1393 2249 635
Size: 758 856 5462
Fragments arranged by size:
5462 856 758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2703 2769 4252 5131 5623 5921 2703
Size: 66 1483 879 492 298 3858
Fragments arranged by size:
3858 1483 879 492 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 1199 1207 2122 2230 2818 6780 1199
Size: 8 915 108 588 3962 1495
Fragments arranged by size:
3962 1495 915 588 108 8
BsaAI yAC'GTr
Cuts at: 900 3536 4429 900
Size: 2636 893 3547
Fragments arranged by size:
3547 2636 893
BsaBI GATnn'nnATC
Cuts at: 6 4590 6627 6
Size: 4584 2037 455
Fragments arranged by size:
4584 2037 455
BsaHI Gr'CG_yC
Cuts at: 462 1066 1163 2719 4507 4755 5457 6953 462
Size: 604 97 1556 1788 248 702 1496 585
Fragments arranged by size:
1788 1556 1496 702 604 585 248 97
BsaWI w'CCGG_w
Cuts at: 1127 1593 3422 3492 4265 4786 5464 5738 5885
Size: 466 1829 70 773 521 678 274 147
Cuts at: 5885 6296 1127
Size: 411 1907
Fragments arranged by size:
1907 1829 773 678 521 466 411 274
147 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1231 1261 1660 1690 2260 2290 2834 2864 3027
Size: 30 399 30 570 30 544 30 163
Cuts at: 3027 3057 1231
Size: 30 5250
Fragments arranged by size:
5250 570 544 399 163 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1306 1381 2772 3076 3094 3136 1306
Size: 75 1391 304 18 42 5246
Fragments arranged by size:
5246 1391 304 75 42 18
BseYI C'CCAG_C
Cuts at: 169 231 879 1089 1465 1631 1828 1948 3328
Size: 62 648 210 376 166 197 120 1380
Cuts at: 3328 3854 4501 5259 5836 6332 169
Size: 526 647 758 577 496 913
Fragments arranged by size:
1380 913 758 648 647 577 526 496
376 210 197 166 120 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 970 2461 3903 268
Size: 702 1491 1442 3441
Fragments arranged by size:
3441 1491 1442 702
BsiEI CG_ry'CG
Cuts at: 104 798 4618 4664 5872 104
Size: 694 3820 46 1208 1308
Fragments arranged by size:
3820 1308 1208 694 46
BsiHKAI G_wGCw'C
Cuts at: 942 1182 1789 2270 3131 3749 4090 4868 5058
Size: 240 607 481 861 618 341 778 190
Cuts at: 5058 5850 942
Size: 792 2168
Fragments arranged by size:
2168 861 792 778 618 607 481 341
240 190
BsmI GAATG_Cn'
Cuts at: 1969 3114 3780 1969
Size: 1145 666 5265
Fragments arranged by size:
5265 1145 666
BsmBI CGTCTCn'nnnn_
Cuts at: 661 661
Size: 7076
Bsp1286I G_dGCh'C
Cuts at: 117 184 218 569 907 942 1090 1182 1262
Size: 67 34 351 338 35 148 92 80
Cuts at: 1262 1283 1726 1789 1799 1854 1885 2270 2512
Size: 21 443 63 10 55 31 385 242
Cuts at: 2512 2689 2782 3040 3131 3742 3749 3795 4090
Size: 177 93 258 91 611 7 46 295
Cuts at: 4090 4701 4794 4868 5058 5120 5850 117
Size: 611 93 74 190 62 730 1343
Fragments arranged by size:
1343 730 611 611 443 385 351 338
295 258 242 190 177 148 93 93
92 91 80 74 67 63 62 55
46 35 34 31 21 10 7
BspEI T'CCGG_A
Cuts at: 6296 6296
Size: 7076
BspHI T'CATG_A
Cuts at: 6252 6252
Size: 7076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3103 4126 4340 4642 5023 3103
Size: 1023 214 302 381 5156
Fragments arranged by size:
5156 1023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3464 5368 5422 6906 7071 50
Size: 3414 1904 54 1484 165 55
Fragments arranged by size:
3414 1904 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 671 3846 3894 4988 671
Size: 3175 48 1094 2759
Fragments arranged by size:
3175 2759 1094 48
BsrFI r'CCGG_y
Cuts at: 928 1073 1132 2610 2883 5074 5255 5464 928
Size: 145 59 1478 273 2191 181 209 2540
Fragments arranged by size:
2540 2191 1478 273 209 181 145 59
BsrGI T'GTAC_A
Cuts at: 2462 2462
Size: 7076
BssHII G'CGCG_C
Cuts at: 932 932
Size: 7076
BssSI C'ACGA_G
Cuts at: 5347 5705 5347
Size: 358 6718
BstAPI GCAn_nnn'nTGC
Cuts at: 156 1492 4453 156
Size: 1336 2961 2779
Fragments arranged by size:
2961 2779 1336
BstBI TT'CG_AA
Cuts at: 3014 5437 3014
Size: 2423 4653
BstEII G'GTnAC_C
Cuts at: 1305 1305
Size: 7076
BstXI CCAn_nnnn'nTGG
Cuts at: 390 1749 1799 390
Size: 1359 50 5667
Fragments arranged by size:
5667 1359 50
BstYI r'GATC_y
Cuts at: 1 1992 2647 3135 4101 4568 4591 4926 5172
Size: 1991 655 488 966 467 23 335 246
Cuts at: 5172 6184 6270 6282 6628 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1991 1012 966 655 488 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2356 2690 2943 2356
Size: 334 253 6489
Fragments arranged by size:
6489 334 253
BtgI C'CryG_G
Cuts at: 107 301 1682 1817 2102 2284 2591 2962 3375
Size: 194 1381 135 285 182 307 371 413
Cuts at: 3375 107
Size: 3808
Fragments arranged by size:
3808 1381 413 371 307 285 194 182
135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5026 5207 5370 5026
Size: 181 163 6732
Fragments arranged by size:
6732 181 163
BtsI GCAGTG_nn'
Cuts at: 442 1490 1661 1713 3773 4319 442
Size: 1048 171 52 2060 546 3199
Fragments arranged by size:
3199 2060 1048 546 171 52
DraI TTT'AAA
Cuts at: 4075 4075
Size: 7076
DraIII CAC_nnn'GTG
Cuts at: 2089 3864 2089
Size: 1775 5301
DrdI GACnn_nn'nnGTC
Cuts at: 4782 5640 4782
Size: 858 6218
EaeI y'GGCC_r
Cuts at: 304 392 416 926 1034 1679 1710 1979 2006
Size: 88 24 510 108 645 31 269 27
Cuts at: 2006 3468 3866 4661 4835 5226 5253 6598 304
Size: 1462 398 795 174 391 27 1345 782
Fragments arranged by size:
1462 1345 795 782 645 510 398 391
269 174 108 88 31 27 27 24
EagI C'GGCC_G
Cuts at: 4661 4661
Size: 7076
EarI CTCTTCn'nnn_
Cuts at: 1292 5099 5309 1292
Size: 3807 210 3059
Fragments arranged by size:
3807 3059 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 277 5284 5594 5740 6886 277
Size: 5007 310 146 1146 467
Fragments arranged by size:
5007 1146 467 310 146
EcoICRI GAG'CTC
Cuts at: 1180 1787 4088 1180
Size: 607 2301 4168
Fragments arranged by size:
4168 2301 607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1309 1781 1882 2205 2256 2262 2534 2755
Size: 12 472 101 323 51 6 272 221
Cuts at: 2755 2831 2958 3040 4901 5333 6080 6348 1297
Size: 76 127 82 1861 432 747 268 2025
Fragments arranged by size:
2025 1861 747 472 432 323 272 268
221 127 101 82 76 51 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1291 2737 1291
Size: 1446 5630
EcoO109I rG'GnC_Cy
Cuts at: 113 114 558 1005 1086 1258 1259 1410 1850
Size: 1 444 447 81 172 1 151 440
Cuts at: 1850 1851 1881 1882 2445 2575 2967 3024 3098
Size: 1 30 1 563 130 392 57 74
Cuts at: 3098 3905 4215 113
Size: 807 310 2974
Fragments arranged by size:
2974 807 563 447 444 440 392 310
172 151 130 81 74 57 30 1
1 1 1
EcoRI G'AATT_C
Cuts at: 4080 4080
Size: 7076
EcoRV GAT'ATC
Cuts at: 192 192
Size: 7076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2426 5095 2426
Size: 2669 4407
FspI TGC'GCA
Cuts at: 1105 4857 1105
Size: 3752 3324
FspAI rTGC'GCAy
Cuts at: 1105 1105
Size: 7076
HaeII r_GCGC'y
Cuts at: 465 1080 1158 1554 1575 1631 3690 4510 4758
Size: 615 78 396 21 56 2059 820 248
Cuts at: 4758 5780 6759 465
Size: 1022 979 782
Fragments arranged by size:
2059 1022 979 820 782 615 396 248
78 56 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1204 1231 1236 1263 4918 4948 4950 4980 1204
Size: 27 5 27 3655 30 2 30 3300
Fragments arranged by size:
3655 3300 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 2256 4115 2256
Size: 1859 5217
KasI G'GCGC_C
Cuts at: 461 4506 4754 461
Size: 4045 248 2783
Fragments arranged by size:
4045 2783 248
KpnI G_GTAC'C
Cuts at: 2530 2962 3834 4096 2530
Size: 432 872 262 5510
Fragments arranged by size:
5510 872 432 262
MluI A'CGCG_T
Cuts at: 6509 6509
Size: 7076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2733 5747 5931 2733
Size: 3014 184 3878
Fragments arranged by size:
3878 3014 184
MscI TGG'CCA
Cuts at: 394 418 1681 1712 1981 2008 3868 4837 394
Size: 24 1263 31 269 27 1860 969 2633
Fragments arranged by size:
2633 1860 1263 969 269 31 27 24
MslI CAynn'nnrTG
Cuts at: 388 1513 1696 5065 5192 388
Size: 1125 183 3369 127 2272
Fragments arranged by size:
3369 2272 1125 183 127
MspA1I CmG'CkG
Cuts at: 524 984 3377 4187 4501 4861 5874 6119 524
Size: 460 2393 810 314 360 1013 245 1481
Fragments arranged by size:
2393 1481 1013 810 460 360 314 245
NaeI GCC'GGC
Cuts at: 930 1075 2612 5257 930
Size: 145 1537 2645 2749
Fragments arranged by size:
2749 2645 1537 145
NarI GG'CG_CC
Cuts at: 462 4507 4755 462
Size: 4045 248 2783
Fragments arranged by size:
4045 2783 248
NcoI C'CATG_G
Cuts at: 107 1682 1817 2284 2591 107
Size: 1575 135 467 307 4592
Fragments arranged by size:
4592 1575 467 307 135
NgoMIV G'CCGG_C
Cuts at: 928 1073 2610 5255 928
Size: 145 1537 2645 2749
Fragments arranged by size:
2749 2645 1537 145
NspI r_CATG'y
Cuts at: 225 3176 4129 4629 5536 225
Size: 2951 953 500 907 1765
Fragments arranged by size:
2951 1765 953 907 500
PasI CC'CwG_GG
Cuts at: 1405 1727 1961 2210 2502 2829 2997 1405
Size: 322 234 249 292 327 168 5484
Fragments arranged by size:
5484 327 322 292 249 234 168
PciI A'CATG_T
Cuts at: 3172 5532 3172
Size: 2360 4716
PflMI CCAn_nnn'nTGG
Cuts at: 425 1225 2030 2285 425
Size: 800 805 255 5216
Fragments arranged by size:
5216 805 800 255
PfoI T'CCnGG_A
Cuts at: 1319 1859 1319
Size: 540 6536
PmeI GTTT'AAAC
Cuts at: 4075 4075
Size: 7076
PmlI CAC'GTG
Cuts at: 3536 3536
Size: 7076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6215 6247 6215
Size: 32 7044
PpuMI rG'GwC_Cy
Cuts at: 558 1410 2445 2575 2967 3024 3098 558
Size: 852 1035 130 392 57 74 4536
Fragments arranged by size:
4536 1035 852 392 130 74 57
PshAI GACnn'nnGTC
Cuts at: 438 3481 438
Size: 3043 4033
PspOMI G'GGCC_C
Cuts at: 113 903 1086 1258 1850 1881 113
Size: 790 183 172 592 31 5308
Fragments arranged by size:
5308 790 592 183 172 31
PstI C_TGCA'G
Cuts at: 152 299 1497 1656 2127 2913 3265 4123 4808
Size: 147 1198 159 471 786 352 858 685
Cuts at: 4808 152
Size: 2420
Fragments arranged by size:
2420 1198 858 786 685 471 352 159
147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7076
PvuII CAG'CTG
Cuts at: 984 4501 4861 984
Size: 3517 360 3199
Fragments arranged by size:
3517 3199 360
RsrII CG'GwC_CG
Cuts at: 1130 5271 1130
Size: 4141 2935
SacI G_AGCT'C
Cuts at: 1182 1789 4090 1182
Size: 607 2301 4168
Fragments arranged by size:
4168 2301 607
SacII CC_GC'GG
Cuts at: 3378 3378
Size: 7076
SalI G'TCGA_C
Cuts at: 4113 4113
Size: 7076
SanDI GG'GwC_CC
Cuts at: 1410 2967 3098 1410
Size: 1557 131 5388
Fragments arranged by size:
5388 1557 131
SapI GCTCTTCn'nnn_
Cuts at: 5099 5309 5099
Size: 210 6866
SbfI CC_TGCA'GG
Cuts at: 4123 4123
Size: 7076
ScaI AGT'ACT
Cuts at: 6593 6593
Size: 7076
SexAI A'CCwGG_T
Cuts at: 560 560
Size: 7076
SfcI C'TryA_G
Cuts at: 33 148 295 691 1493 1652 2123 2312 2429
Size: 115 147 396 802 159 471 189 117
Cuts at: 2429 2564 2909 3201 3261 4119 4804 5797 5988
Size: 135 345 292 60 858 685 993 191
Cuts at: 5988 33
Size: 1121
Fragments arranged by size:
1121 993 858 802 685 471 396 345
292 191 189 159 147 135 117 115
60
SfiI GGCCn_nnn'nGGCC
Cuts at: 646 646
Size: 7076
SfoI GGC'GCC
Cuts at: 463 4508 4756 463
Size: 4045 248 2783
Fragments arranged by size:
4045 2783 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7076
SgrAI Cr'CCGG_yG
Cuts at: 1073 1073
Size: 7076
SmaI CCC'GGG
Cuts at: 610 874 1025 1097 2369 3041 4098 610
Size: 264 151 72 1272 672 1057 3588
Fragments arranged by size:
3588 1272 1057 672 264 151 72
SmlI C'TyrA_G
Cuts at: 2682 2784 4231 5146 5638 5900 2682
Size: 102 1447 915 492 262 3858
Fragments arranged by size:
3858 1447 915 492 262 102
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7076
SphI G_CATG'C
Cuts at: 4129 4629 4129
Size: 500 6576
StuI AGG'CCT
Cuts at: 649 649
Size: 7076
StyI C'CwwG_G
Cuts at: 107 131 344 395 569 1537 1682 1817 1925
Size: 24 213 51 174 968 145 135 108
Cuts at: 1925 2132 2284 2554 2591 2874 4210 4564 107
Size: 207 152 270 37 283 1336 354 2619
Fragments arranged by size:
2619 1336 968 354 283 270 213 207
174 152 145 135 108 51 37 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 1842 2816 4046 4659 4792 5251 5460 784
Size: 1058 974 1230 613 133 459 209 2400
Fragments arranged by size:
2400 1230 1058 974 613 459 209 133
TatI w'GTAC_w
Cuts at: 2462 3806 6591 2462
Size: 1344 2785 2947
Fragments arranged by size:
2947 2785 1344
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2260 2292 2567 2571 2599 2603 2639 2671 2834
Size: 32 275 4 28 4 36 32 163
Cuts at: 2834 2866 3027 3059 3982 4014 6593 6625 2260
Size: 32 161 32 923 32 2579 32 2711
Fragments arranged by size:
2711 2579 923 275 163 161 36 32
32 32 32 32 32 28 4 4
Tth111I GACn'n_nGTC
Cuts at: 357 403 4873 357
Size: 46 4470 2560
Fragments arranged by size:
4470 2560 46
XbaI T'CTAG_A
Cuts at: 4107 4107
Size: 7076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 778 1913 2772 4040 4571 483
Size: 295 1135 859 1268 531 2988
Fragments arranged by size:
2988 1268 1135 859 531 295
XmaI C'CCGG_G
Cuts at: 608 872 1023 1095 2367 3039 4096 608
Size: 264 151 72 1272 672 1057 3588
Fragments arranged by size:
3588 1272 1057 672 264 151 72
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI BaeI BcgI BfrBI BmtI
BsiWI BstZ17I ClaI CspCI FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PspXI
PsrI SnaBI SrfI SspI SwaI XhoI XmnI ZraI