Restriction map for
pF1KB1487
(Circular) (Six-base) MAPSORT of: pF1KB1487.seq Check: 421 from: 1 to: 8852
pF1KB1487 8852 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1795 1861 1869 2197 5755 5788 1795
Size: 66 8 328 3558 33 4859
Fragments arranged by size:
4859 3558 328 66 33 8
AccI GT'mk_AC
Cuts at: 418 5890 418
Size: 5472 3380
Acc65I G'GTAC_C
Cuts at: 5868 5868
Size: 8852
AclI AA'CG_TT
Cuts at: 5123 8692 5123
Size: 3569 5283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1105 1457 1687 2084 2404 2746 2872 3149
Size: 575 352 230 397 320 342 126 277
Cuts at: 3149 3232 3268 4412 4981 5018 5227 5233 5492
Size: 83 36 1144 569 37 209 6 259
Cuts at: 5492 6677 7109 7856 8124 530
Size: 1185 432 747 268 1258
Fragments arranged by size:
1258 1185 1144 747 575 569 432 397
352 342 320 277 268 259 230 209
126 83 37 36 6
AfeI AGC'GCT
Cuts at: 2208 2208
Size: 8852
AflIII A'CryG_T
Cuts at: 800 2432 4331 5036 6204 7308 8285 800
Size: 1632 1899 705 1168 1104 977 1367
Fragments arranged by size:
1899 1632 1367 1168 1104 977 705
AgeI A'CCGG_T
Cuts at: 7240 7240
Size: 8852
AleI CACnn'nnGTG
Cuts at: 1465 4087 1465
Size: 2622 6230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 734 3466 734
Size: 2732 6120
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 839 871 3271 3303 839
Size: 32 2400 32 6388
Fragments arranged by size:
6388 2400 32 32
AlwNI CAG_nnn'CTG
Cuts at: 759 1895 3417 4287 6157 7724 759
Size: 1136 1522 870 1870 1567 1887
Fragments arranged by size:
1887 1870 1567 1522 1136 870
ApaI G_GGCC'C
Cuts at: 3173 3173
Size: 8852
ApaLI G'TGCA_C
Cuts at: 2362 2941 4852 7622 2362
Size: 579 1911 2770 3592
Fragments arranged by size:
3592 2770 1911 579
ApoI r'AATT_y
Cuts at: 1351 4528 4984 5856 8205 8461 1351
Size: 3177 456 872 2349 256 1742
Fragments arranged by size:
3177 2349 1742 872 456 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8852
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8852
AvaI C'yCGr_G
Cuts at: 4441 5617 5872 4441
Size: 1176 255 7421
Fragments arranged by size:
7421 1176 255
BamHI G'GATC_C
Cuts at: 165 5877 165
Size: 5712 3140
BanI G'GyrC_C
Cuts at: 1459 1843 2260 3018 4900 4975 5868 6282 6530
Size: 384 417 758 1882 75 893 414 248
Cuts at: 6530 6565 1459
Size: 35 3746
Fragments arranged by size:
3746 1882 893 758 417 414 384 248
75 35
BanII G_rGCy'C
Cuts at: 293 1811 2562 3173 3733 4144 4442 5018 5408
Size: 1518 751 611 560 411 298 576 390
Cuts at: 5408 5795 5866 6896 293
Size: 387 71 1030 2249
Fragments arranged by size:
2249 1518 1030 751 611 576 560 411
390 387 298 71
BbeI G_GCGC'C
Cuts at: 6286 6534 6286
Size: 248 8604
BbsI GAAGACnn'nnnn_
Cuts at: 1132 2705 2849 3464 5382 1132
Size: 1573 144 615 1918 4602
Fragments arranged by size:
4602 1918 1573 615 144
BbvCI CC'TCA_GC
Cuts at: 1049 5130 5507 1049
Size: 4081 377 4394
Fragments arranged by size:
4394 4081 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 632 666 632
Size: 34 8818
BciVI GTATCCnnnnn_n'
Cuts at: 2161 5599 6750 7511 8852 2161
Size: 3438 1151 761 1341 2161
Fragments arranged by size:
3438 2161 1341 1151 761
BclI T'GATC_A
Cuts at: 5003 6372 5003
Size: 1369 7483
BglI GCCn_nnn'nGGC
Cuts at: 1801 2664 3168 1801
Size: 863 504 7485
Fragments arranged by size:
7485 863 504
BglII A'GATC_T
Cuts at: 4214 4811 5168 6367 8404 4214
Size: 597 357 1199 2037 4662
Fragments arranged by size:
4662 2037 1199 597 357
BlpI GC'TnA_GC
Cuts at: 1805 5964 1805
Size: 4159 4693
Bme1580I G_kGCm'C
Cuts at: 586 723 1736 2182 2366 2945 3173 4856 4903
Size: 137 1013 446 184 579 228 1683 47
Cuts at: 4903 6477 6570 7626 586
Size: 1574 93 1056 1812
Fragments arranged by size:
1812 1683 1574 1056 1013 579 446 228
184 137 93 47
BmgBI CAC'GTC
Cuts at: 789 2433 2712 4238 789
Size: 1644 279 1526 5403
Fragments arranged by size:
5403 1644 1526 279
BmrI ACTGGGnnnn_n'
Cuts at: 1723 6064 6145 6480 8624 1723
Size: 4341 81 335 2144 1951
Fragments arranged by size:
4341 2144 1951 335 81
BmtI G_CTAG'C
Cuts at: 3296 3296
Size: 8852
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1247 2150 7219 1247
Size: 903 5069 2880
Fragments arranged by size:
5069 2880 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 984 991 1093 1229 1641 2231 3442 3662 4741
Size: 7 102 136 412 590 1211 220 1079
Cuts at: 4741 4963 984
Size: 222 4873
Fragments arranged by size:
4873 1211 1079 590 412 222 220 136
102 7
Bpu10I CC'TnA_GC
Cuts at: 1049 3148 4664 5130 5507 1049
Size: 2099 1516 466 377 4394
Fragments arranged by size:
4394 2099 1516 466 377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 676 2479 3292 3505 4849 5515 6028 6907
Size: 520 1803 813 213 1344 666 513 879
Cuts at: 6907 7399 7697 156
Size: 492 298 1311
Fragments arranged by size:
1803 1344 1311 879 813 666 520 513
492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 550 2795 3103 3199 3235 4961 8556 550
Size: 2245 308 96 36 1726 3595 846
Fragments arranged by size:
3595 2245 1726 846 308 96 36
BsaAI yAC'GTr
Cuts at: 803 2183 4321 6205 803
Size: 1380 2138 1884 3450
Fragments arranged by size:
3450 2138 1884 1380
BsaBI GATnn'nnATC
Cuts at: 6 4518 4639 6366 8403 6
Size: 4512 121 1727 2037 455
Fragments arranged by size:
4512 2037 1727 455 121
BsaHI Gr'CG_yC
Cuts at: 6283 6531 7233 8729 6283
Size: 248 702 1496 6406
Fragments arranged by size:
6406 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1113 4454 5309 6041 6562 7240 7514 7661 8072
Size: 3341 855 732 521 678 274 147 411
Cuts at: 8072 1113
Size: 1893
Fragments arranged by size:
3341 1893 855 732 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1765 1795 3854 3884 4714 4744 4774 4804 1765
Size: 30 2059 30 830 30 30 30 5813
Fragments arranged by size:
5813 2059 830 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 1223 1485 2624 2707 2773 4134 4161 4522
Size: 808 262 1139 83 66 1361 27 361
Cuts at: 4522 415
Size: 4745
Fragments arranged by size:
4745 1361 1139 808 361 262 83 66
27
BseYI C'CCAG_C
Cuts at: 606 1957 2264 2862 2951 3060 3399 3483 4053
Size: 1351 307 598 89 109 339 84 570
Cuts at: 4053 4107 6277 7035 7612 8108 606
Size: 54 2170 758 577 496 1350
Fragments arranged by size:
2170 1351 1350 758 598 577 570 496
339 307 109 89 84 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1551 1955 2062 2479 5220 5766 1551
Size: 404 107 417 2741 546 4637
Fragments arranged by size:
4637 2741 546 417 404 107
BsiEI CG_ry'CG
Cuts at: 104 6394 6440 7648 104
Size: 6290 46 1208 1308
Fragments arranged by size:
6290 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 450 1182 1714 1811 2237 2366 2945 3652 3733
Size: 732 532 97 426 129 579 707 81
Cuts at: 3733 3956 4019 4144 4856 5795 5866 6644 6834
Size: 223 63 125 712 939 71 778 190
Cuts at: 6834 7626 450
Size: 792 1676
Fragments arranged by size:
1676 939 792 778 732 712 707 579
532 426 223 190 129 125 97 81
71 63
BsiWI C'GTAC_G
Cuts at: 1716 1716
Size: 8852
BsmI GAATG_Cn'
Cuts at: 1342 1872 2518 3123 4953 5158 1342
Size: 530 646 605 1830 205 5036
Fragments arranged by size:
5036 1830 646 605 530 205
BsmBI CGTCTCn'nnnn_
Cuts at: 2734 3576 2734
Size: 842 8010
Bsp1286I G_dGCh'C
Cuts at: 293 450 586 723 1182 1714 1736 1811 2182
Size: 157 136 137 459 532 22 75 371
Cuts at: 2182 2237 2366 2562 2945 3173 3652 3733 3956
Size: 55 129 196 383 228 479 81 223
Cuts at: 3956 4019 4144 4442 4856 4903 5018 5408 5795
Size: 63 125 298 414 47 115 390 387
Cuts at: 5795 5866 6477 6570 6644 6834 6896 7626 293
Size: 71 611 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 532 479 459 414 390
387 383 371 298 228 223 196 190
157 137 136 129 125 115 93 81
75 74 71 63 62 55 47 22
BspEI T'CCGG_A
Cuts at: 8072 8072
Size: 8852
BspHI T'CATG_A
Cuts at: 4643 8028 4643
Size: 3385 5467
BspMI ACCTGCnnnn'nnnn_
Cuts at: 669 744 1795 1861 1869 2197 2900 2946 3042
Size: 75 1051 66 8 328 703 46 96
Cuts at: 3042 3792 4295 5755 5788 5902 6116 6418 6799
Size: 750 503 1460 33 114 214 302 381
Cuts at: 6799 669
Size: 2722
Fragments arranged by size:
2722 1460 1051 750 703 503 381 328
302 214 114 96 75 66 46 33
8
BsrBI CCG'CTC
Cuts at: 50 5448 7144 7198 8682 8847 50
Size: 5398 1696 54 1484 165 55
Fragments arranged by size:
5398 1696 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3159 3267 3818 5361 5399 6764 3159
Size: 108 551 1543 38 1365 5247
Fragments arranged by size:
5247 1543 1365 551 108 38
BsrFI r'CCGG_y
Cuts at: 665 1795 2473 6850 7031 7240 665
Size: 1130 678 4377 181 209 2277
Fragments arranged by size:
4377 2277 1130 678 209 181
BsrGI T'GTAC_A
Cuts at: 797 4194 797
Size: 3397 5455
BssHII G'CGCG_C
Cuts at: 4006 4006
Size: 8852
BssSI C'ACGA_G
Cuts at: 7123 7481 7123
Size: 358 8494
BstAPI GCAn_nnn'nTGC
Cuts at: 995 3417 5243 6229 995
Size: 2422 1826 986 3618
Fragments arranged by size:
3618 2422 1826 986
BstBI TT'CG_AA
Cuts at: 263 7213 263
Size: 6950 1902
BstEII G'GTnAC_C
Cuts at: 280 681 930 1899 2344 5432 280
Size: 401 249 969 445 3088 3700
Fragments arranged by size:
3700 3088 969 445 401 249
BstXI CCAn_nnnn'nTGG
Cuts at: 1015 3129 4941 5283 1015
Size: 2114 1812 342 4584
Fragments arranged by size:
4584 2114 1812 342
BstYI r'GATC_y
Cuts at: 1 165 2093 2486 2858 3938 4214 4519 4811
Size: 164 1928 393 372 1080 276 305 292
Cuts at: 4811 5168 5877 6344 6367 6702 6948 7960 8046
Size: 357 709 467 23 335 246 1012 86
Cuts at: 8046 8058 8404 1
Size: 12 346 449
Fragments arranged by size:
1928 1080 1012 709 467 449 393 372
357 346 335 305 292 276 246 164
86 23 12
BstZ17I GTA'TAC
Cuts at: 419 419
Size: 8852
Bsu36I CC'TnA_GG
Cuts at: 525 2653 3593 3839 525
Size: 2128 940 246 5538
Fragments arranged by size:
5538 2128 940 246
BtgI C'CryG_G
Cuts at: 107 768 1319 1839 2566 3182 3254 3665 5257
Size: 661 551 520 727 616 72 411 1592
Cuts at: 5257 107
Size: 3702
Fragments arranged by size:
3702 1592 727 661 616 551 520 411
72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6802 6983 7146 6802
Size: 181 163 8508
Fragments arranged by size:
8508 181 163
BtsI GCAGTG_nn'
Cuts at: 447 557 706 1568 1714 1967 3108 3134 3586
Size: 110 149 862 146 253 1141 26 452
Cuts at: 3586 4017 4033 4194 5248 6095 447
Size: 431 16 161 1054 847 3204
Fragments arranged by size:
3204 1141 1054 862 847 452 431 253
161 149 146 110 26 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 423 458 423
Size: 35 8817
DraI TTT'AAA
Cuts at: 5851 5851
Size: 8852
DraIII CAC_nnn'GTG
Cuts at: 4123 5243 4123
Size: 1120 7732
DrdI GACnn_nn'nnGTC
Cuts at: 6558 7416 6558
Size: 858 7994
EaeI y'GGCC_r
Cuts at: 663 2403 2475 2665 2978 3636 4001 4931 5117
Size: 1740 72 190 313 658 365 930 186
Cuts at: 5117 6437 6611 7002 7029 8374 663
Size: 1320 174 391 27 1345 1141
Fragments arranged by size:
1740 1345 1320 1141 930 658 391 365
313 190 186 174 72 27
EagI C'GGCC_G
Cuts at: 6437 6437
Size: 8852
EarI CTCTTCn'nnn_
Cuts at: 1208 1216 1912 1924 2722 2924 3740 4388 5828
Size: 8 696 12 798 202 816 648 1440
Cuts at: 5828 5837 6875 7085 1208
Size: 9 1038 210 2975
Fragments arranged by size:
2975 1440 1038 816 798 696 648 210
202 12 9 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2758 3658 7060 7370 7516 8662 2758
Size: 900 3402 310 146 1146 2948
Fragments arranged by size:
3402 2948 1146 900 310 146
EcoICRI GAG'CTC
Cuts at: 1809 3731 4142 5793 5864 1809
Size: 1922 411 1651 71 4797
Fragments arranged by size:
4797 1922 1651 411 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 984 991 1093 1105 1229 1457 1641 1687
Size: 454 7 102 12 124 228 184 46
Cuts at: 1687 2084 2231 2404 2746 2872 3149 3232 3268
Size: 397 147 173 342 126 277 83 36
Cuts at: 3268 3442 3662 4412 4741 4963 4981 5018 5227
Size: 174 220 750 329 222 18 37 209
Cuts at: 5227 5233 5492 6677 7109 7856 8124 530
Size: 6 259 1185 432 747 268 1258
Fragments arranged by size:
1258 1185 750 747 454 432 397 342
329 277 268 259 228 222 220 209
184 174 173 147 126 124 102 83
46 37 36 18 12 7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 196 1831 3547 196
Size: 1635 1716 5501
Fragments arranged by size:
5501 1716 1635
EcoO109I rG'GnC_Cy
Cuts at: 201 1194 1724 1989 2595 2648 3169 3170 3699
Size: 993 530 265 606 53 521 1 529
Cuts at: 3699 4284 4965 5991 201
Size: 585 681 1026 3062
Fragments arranged by size:
3062 1026 993 681 606 585 530 529
521 265 53 1
EcoRI G'AATT_C
Cuts at: 5856 5856
Size: 8852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4986 5509 6871 4986
Size: 523 1362 6967
Fragments arranged by size:
6967 1362 523
FseI GG_CCGG'CC
Cuts at: 669 669
Size: 8852
FspI TGC'GCA
Cuts at: 6633 6633
Size: 8852
HaeII r_GCGC'y
Cuts at: 1895 2210 6286 6534 7556 8535 1895
Size: 315 4076 248 1022 979 2212
Fragments arranged by size:
4076 2212 1022 979 315 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 605 637 1495 1527 2953 2985 3037 3069 3697
Size: 32 858 32 1426 32 52 32 628
Cuts at: 3697 3729 3882 3914 4714 4746 5155 5187 5629
Size: 32 153 32 800 32 409 32 442
Cuts at: 5629 5661 6694 6724 6726 6756 605
Size: 32 1033 30 2 30 2701
Fragments arranged by size:
2701 1426 1033 858 800 628 442 409
153 52 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 180 470 777 4077 4890 5760 5891 180
Size: 290 307 3300 813 870 131 3141
Fragments arranged by size:
3300 3141 870 813 307 290 131
HindIII A'AGCT_T
Cuts at: 5486 5486
Size: 8852
KasI G'GCGC_C
Cuts at: 6282 6530 6282
Size: 248 8604
KpnI G_GTAC'C
Cuts at: 5872 5872
Size: 8852
MluI A'CGCG_T
Cuts at: 8285 8285
Size: 8852
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1601 5198 7523 7707 1601
Size: 3597 2325 184 2746
Fragments arranged by size:
3597 2746 2325 184
MscI TGG'CCA
Cuts at: 2405 2667 2980 3638 4933 5119 6613 2405
Size: 262 313 658 1295 186 1494 4644
Fragments arranged by size:
4644 1494 1295 658 313 262 186
MslI CAynn'nnrTG
Cuts at: 1465 3961 4087 4711 5035 6841 6968 1465
Size: 2496 126 624 324 1806 127 3349
Fragments arranged by size:
3349 2496 1806 624 324 127 126
MspA1I CmG'CkG
Cuts at: 332 844 1565 2588 2790 2898 4111 5963 6277
Size: 512 721 1023 202 108 1213 1852 314
Cuts at: 6277 6637 7650 7895 332
Size: 360 1013 245 1289
Fragments arranged by size:
1852 1289 1213 1023 1013 721 512 360
314 245 202 108
NaeI GCC'GGC
Cuts at: 667 1797 2475 7033 667
Size: 1130 678 4558 2486
Fragments arranged by size:
4558 2486 1130 678
NarI GG'CG_CC
Cuts at: 6283 6531 6283
Size: 248 8604
NcoI C'CATG_G
Cuts at: 107 768 1319 1839 3254 5257 107
Size: 661 551 520 1415 2003 3702
Fragments arranged by size:
3702 2003 1415 661 551 520
NdeI CA'TA_TG
Cuts at: 4176 4176
Size: 8852
NgoMIV G'CCGG_C
Cuts at: 665 1795 2473 7031 665
Size: 1130 678 4558 2486
Fragments arranged by size:
4558 2486 1130 678
NheI G'CTAG_C
Cuts at: 3292 3292
Size: 8852
NruI TCG'CGA
Cuts at: 5552 5552
Size: 8852
NspI r_CATG'y
Cuts at: 753 2473 4259 4806 5040 5905 6405 7312 753
Size: 1720 1786 547 234 865 500 907 2293
Fragments arranged by size:
2293 1786 1720 907 865 547 500 234
PasI CC'CwG_GG
Cuts at: 1028 2307 1028
Size: 1279 7573
PciI A'CATG_T
Cuts at: 5036 7308 5036
Size: 2272 6580
PflMI CCAn_nnn'nTGG
Cuts at: 864 3497 4680 864
Size: 2633 1183 5036
Fragments arranged by size:
5036 2633 1183
PfoI T'CCnGG_A
Cuts at: 1420 1451 1817 2153 2165 3715 3941 4280 4718
Size: 31 366 336 12 1550 226 339 438
Cuts at: 4718 1420
Size: 5554
Fragments arranged by size:
5554 1550 438 366 339 336 226 31
12
PmeI GTTT'AAAC
Cuts at: 5851 5851
Size: 8852
PmlI CAC'GTG
Cuts at: 803 803
Size: 8852
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2451 7991 8023 2419
Size: 32 5540 32 3248
Fragments arranged by size:
5540 3248 32 32
PpuMI rG'GwC_Cy
Cuts at: 1194 1724 2595 2648 4284 4965 1194
Size: 530 871 53 1636 681 5081
Fragments arranged by size:
5081 1636 871 681 530 53
PshAI GACnn'nnGTC
Cuts at: 1114 4925 1114
Size: 3811 5041
PspOMI G'GGCC_C
Cuts at: 3169 3169
Size: 8852
PstI C_TGCA'G
Cuts at: 713 981 1151 1709 1891 1988 2897 5899 6584
Size: 268 170 558 182 97 909 3002 685
Cuts at: 6584 713
Size: 2981
Fragments arranged by size:
3002 2981 909 685 558 268 182 170
97
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8852
PvuII CAG'CTG
Cuts at: 844 1565 2588 2790 4111 6277 6637 844
Size: 721 1023 202 1321 2166 360 3059
Fragments arranged by size:
3059 2166 1321 1023 721 360 202
RsrII CG'GwC_CG
Cuts at: 7047 7047
Size: 8852
SacI G_AGCT'C
Cuts at: 1811 3733 4144 5795 5866 1811
Size: 1922 411 1651 71 4797
Fragments arranged by size:
4797 1922 1651 411 71
SalI G'TCGA_C
Cuts at: 5889 5889
Size: 8852
SanDI GG'GwC_CC
Cuts at: 1724 2648 1724
Size: 924 7928
SapI GCTCTTCn'nnn_
Cuts at: 4388 6875 7085 4388
Size: 2487 210 6155
Fragments arranged by size:
6155 2487 210
SbfI CC_TGCA'GG
Cuts at: 1151 1988 5899 1151
Size: 837 3911 4104
Fragments arranged by size:
4104 3911 837
ScaI AGT'ACT
Cuts at: 8369 8369
Size: 8852
SfcI C'TryA_G
Cuts at: 33 709 977 1147 1599 1705 1887 1984 2539
Size: 676 268 170 452 106 182 97 555
Cuts at: 2539 2893 4203 5895 6580 7573 7764 33
Size: 354 1310 1692 685 993 191 1121
Fragments arranged by size:
1692 1310 1121 993 685 676 555 452
354 268 191 182 170 106 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 2664 2664
Size: 8852
SfoI GGC'GCC
Cuts at: 6284 6532 6284
Size: 248 8604
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8852
SmaI CCC'GGG
Cuts at: 5874 5874
Size: 8852
SmlI C'TyrA_G
Cuts at: 171 691 2494 3307 3520 4864 5494 6007 6922
Size: 520 1803 813 213 1344 630 513 915
Cuts at: 6922 7414 7676 171
Size: 492 262 1347
Fragments arranged by size:
1803 1347 1344 915 813 630 520 513
492 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8852
SphI G_CATG'C
Cuts at: 753 2473 5905 6405 753
Size: 1720 3432 500 3200
Fragments arranged by size:
3432 3200 1720 500
StuI AGG'CCT
Cuts at: 832 1187 2197 2658 832
Size: 355 1010 461 7026
Fragments arranged by size:
7026 1010 461 355
StyI C'CwwG_G
Cuts at: 107 768 858 1057 1319 1584 1839 1871 3122
Size: 661 90 199 262 265 255 32 1251
Cuts at: 3122 3254 3695 5114 5183 5257 5303 5986 6340
Size: 132 441 1419 69 74 46 683 354
Cuts at: 6340 107
Size: 2619
Fragments arranged by size:
2619 1419 1251 683 661 441 354 265
262 255 199 132 90 74 69 46
32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1989 2239 2278 3052 6435 6568 7027 7236 1989
Size: 250 39 774 3383 133 459 209 3605
Fragments arranged by size:
3605 3383 774 459 250 209 133 39
TatI w'GTAC_w
Cuts at: 797 2824 4194 4799 4829 5321 5741 8367 797
Size: 2027 1370 605 30 492 420 2626 1282
Fragments arranged by size:
2626 2027 1370 1282 605 492 420 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1964 1996 3854 3886 4774 4806 8369 8401 1964
Size: 32 1858 32 888 32 3563 32 2415
Fragments arranged by size:
3563 2415 1858 888 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2919 4155 6649 2919
Size: 1236 2494 5122
Fragments arranged by size:
5122 2494 1236
XbaI T'CTAG_A
Cuts at: 5883 5883
Size: 8852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 861 4710 6347 861
Size: 3849 1637 3366
Fragments arranged by size:
3849 3366 1637
XmaI C'CCGG_G
Cuts at: 5872 5872
Size: 8852
XmnI GAAnn'nnTTC
Cuts at: 4588 4588
Size: 8852
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NruI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BfrBI ClaI
EcoRV FspAI HpaI MfeI NotI NsiI PacI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI