Restriction map for
pF1KB1393
(Circular) (Six-base) MAPSORT of: pF1KB1393.seq Check: 2864 from: 1 to: 5543
pF1KB1393 5543 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 237 237
Size: 5543
AccI GT'mk_AC
Cuts at: 2462 2581 2462
Size: 119 5424
Acc65I G'GTAC_C
Cuts at: 2559 2559
Size: 5543
AclI AA'CG_TT
Cuts at: 5383 5383
Size: 5543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 365 434 448 611 944 2030 3368 3800
Size: 196 69 14 163 333 1086 1338 432
Cuts at: 3800 4153 4815 169
Size: 353 662 897
Fragments arranged by size:
1338 1086 897 662 432 353 333 196
163 69 14
AflIII A'CryG_T
Cuts at: 2895 4695 4976 2895
Size: 1800 281 3462
Fragments arranged by size:
3462 1800 281
AleI CACnn'nnGTG
Cuts at: 173 1315 173
Size: 1142 4401
AlwNI CAG_nnn'CTG
Cuts at: 366 2848 4286 366
Size: 2482 1438 1623
Fragments arranged by size:
2482 1623 1438
ApaLI G'TGCA_C
Cuts at: 4381 4381
Size: 5543
ApoI r'AATT_y
Cuts at: 710 1449 2547 4896 5152 710
Size: 739 1098 2349 256 1101
Fragments arranged by size:
2349 1101 1098 739 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5543
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5543
AvaI C'yCGr_G
Cuts at: 2563 2563
Size: 5543
BamHI G'GATC_C
Cuts at: 2568 2568
Size: 5543
BanI G'GyrC_C
Cuts at: 515 2231 2559 2973 3221 3256 515
Size: 1716 328 414 248 35 2802
Fragments arranged by size:
2802 1716 414 328 248 35
BanII G_rGCy'C
Cuts at: 512 1017 1499 2432 2557 3587 512
Size: 505 482 933 125 1030 2468
Fragments arranged by size:
2468 1030 933 505 482 125
BbeI G_GCGC'C
Cuts at: 2235 2977 3225 2235
Size: 742 248 4553
Fragments arranged by size:
4553 742 248
BbsI GAAGACnn'nnnn_
Cuts at: 1049 1049
Size: 5543
BbvCI CC'TCA_GC
Cuts at: 1243 2482 1243
Size: 1239 4304
BciVI GTATCCnnnnn_n'
Cuts at: 3441 4497 5543 3441
Size: 1056 1046 3441
Fragments arranged by size:
3441 1056 1046
BclI T'GATC_A
Cuts at: 3063 3063
Size: 5543
BglI GCCn_nnn'nGGC
Cuts at: 586 586
Size: 5543
BglII A'GATC_T
Cuts at: 3058 5095 3058
Size: 2037 3506
BlpI GC'TnA_GC
Cuts at: 370 2655 370
Size: 2285 3258
Bme1580I G_kGCm'C
Cuts at: 1275 1351 3168 3261 4385 1275
Size: 76 1817 93 1124 2433
Fragments arranged by size:
2433 1817 1124 93 76
BmgBI CAC'GTC
Cuts at: 214 214
Size: 5543
BmrI ACTGGGnnnn_n'
Cuts at: 1090 1572 1839 2186 2426 2755 2836 3171 5315
Size: 482 267 347 240 329 81 335 2144
Cuts at: 5315 1090
Size: 1318
Fragments arranged by size:
2144 1318 482 347 335 329 267 240
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1513 3910 1513
Size: 2397 3146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2182 1630
Size: 552 4991
Bpu10I CC'TnA_GC
Cuts at: 511 1243 2482 511
Size: 732 1239 3572
Fragments arranged by size:
3572 1239 732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 418 821 1068 2719 3598 4312 4610 406
Size: 12 403 247 1651 879 714 298 1339
Fragments arranged by size:
1651 1339 879 714 403 298 247 12
BsaI GGTCTCn'nnnn_
Cuts at: 290 382 695 5247 290
Size: 92 313 4552 586
Fragments arranged by size:
4552 586 313 92
BsaAI yAC'GTr
Cuts at: 1974 2896 1974
Size: 922 4621
BsaBI GATnn'nnATC
Cuts at: 6 3057 5094 6
Size: 3051 2037 455
Fragments arranged by size:
3051 2037 455
BsaHI Gr'CG_yC
Cuts at: 1223 1559 2232 2974 3222 3924 5420 1223
Size: 336 673 742 248 702 1496 1346
Fragments arranged by size:
1496 1346 742 702 673 336 248
BsaWI w'CCGG_w
Cuts at: 806 879 2268 2732 3253 3931 4342 4489 4763
Size: 73 1389 464 521 678 411 147 274
Cuts at: 4763 806
Size: 1586
Fragments arranged by size:
1586 1389 678 521 464 411 274 147
73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1254 1284 1771 1801 1254
Size: 30 487 30 4996
Fragments arranged by size:
4996 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 487 508 793 1190 1361 1795 2215 487
Size: 21 285 397 171 434 420 3815
Fragments arranged by size:
3815 434 420 397 285 171 21
BseYI C'CCAG_C
Cuts at: 599 1301 1467 2968 3726 4391 4799 599
Size: 702 166 1501 758 665 408 1343
Fragments arranged by size:
1501 1343 758 702 665 408 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1775 1993 631
Size: 1144 218 4181
Fragments arranged by size:
4181 1144 218
BsiEI CG_ry'CG
Cuts at: 104 3085 3131 4361 104
Size: 2981 46 1230 1286
Fragments arranged by size:
2981 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 512 1136 1588 2557 3335 3525 4385 512
Size: 624 452 969 778 190 860 1670
Fragments arranged by size:
1670 969 860 778 624 452 190
BsmI GAATG_Cn'
Cuts at: 1892 1892
Size: 5543
Bsp1286I G_dGCh'C
Cuts at: 512 1017 1136 1275 1351 1499 1588 2432 2557
Size: 505 119 139 76 148 89 844 125
Cuts at: 2557 3168 3261 3335 3525 3587 4385 512
Size: 611 93 74 190 62 798 1670
Fragments arranged by size:
1670 844 798 611 505 190 148 139
125 119 93 89 76 74 62
BspEI T'CCGG_A
Cuts at: 2268 2268
Size: 5543
BspHI T'CATG_A
Cuts at: 1863 3975 1863
Size: 2112 3431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 2593 2807 3109 3490 237
Size: 2356 214 302 381 2290
Fragments arranged by size:
2356 2290 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3835 3889 5373 5538 50
Size: 3785 54 1484 165 55
Fragments arranged by size:
3785 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 985 3455 985
Size: 2470 3073
BsrFI r'CCGG_y
Cuts at: 2012 3541 3722 2012
Size: 1529 181 3833
Fragments arranged by size:
3833 1529 181
BsrGI T'GTAC_A
Cuts at: 2055 2055
Size: 5543
BssSI C'ACGA_G
Cuts at: 141 3814 4522 141
Size: 3673 708 1162
Fragments arranged by size:
3673 1162 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2113 2920 2113
Size: 807 4736
BstBI TT'CG_AA
Cuts at: 3904 3904
Size: 5543
BstYI r'GATC_y
Cuts at: 1 2568 3035 3058 3393 3639 3945 3957 4043
Size: 2567 467 23 335 246 306 12 86
Cuts at: 4043 5095 1
Size: 1052 449
Fragments arranged by size:
2567 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1691 2227 1691
Size: 536 5007
BtgI C'CryG_G
Cuts at: 107 1226 2142 107
Size: 1119 916 3508
Fragments arranged by size:
3508 1119 916
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3493 3674 3837 3493
Size: 181 163 5199
Fragments arranged by size:
5199 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 1657 1760 1792 2001 2786 1310
Size: 347 103 32 209 785 4067
Fragments arranged by size:
4067 785 347 209 103 32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 968 1003 968
Size: 35 5508
DraI TTT'AAA
Cuts at: 936 2542 936
Size: 1606 3937
DraIII CAC_nnn'GTG
Cuts at: 327 2076 327
Size: 1749 3794
DrdI GACnn_nn'nnGTC
Cuts at: 417 3249 4593 417
Size: 2832 1344 1367
Fragments arranged by size:
2832 1367 1344
EaeI y'GGCC_r
Cuts at: 1257 1665 2017 2344 3128 3302 3693 3720 5065
Size: 408 352 327 784 174 391 27 1345
Cuts at: 5065 1257
Size: 1735
Fragments arranged by size:
1735 1345 784 408 391 352 327 174
27
EagI C'GGCC_G
Cuts at: 3128 3128
Size: 5543
EarI CTCTTCn'nnn_
Cuts at: 145 341 530 1924 3566 3776 145
Size: 196 189 1394 1642 210 1912
Fragments arranged by size:
1912 1642 1394 210 196 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1144 3751 4493 4639 5353 1144
Size: 2607 742 146 714 1334
Fragments arranged by size:
2607 1334 742 714 146
EcoICRI GAG'CTC
Cuts at: 510 2555 510
Size: 2045 3498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 365 434 448 611 944 1630 2030 2182
Size: 196 69 14 163 333 686 400 152
Cuts at: 2182 3368 3800 4153 4815 169
Size: 1186 432 353 662 897
Fragments arranged by size:
1186 897 686 662 432 400 353 333
196 163 152 69 14
EcoO109I rG'GnC_Cy
Cuts at: 155 841 918 1360 2478 2682 155
Size: 686 77 442 1118 204 3016
Fragments arranged by size:
3016 1118 686 442 204 77
EcoRI G'AATT_C
Cuts at: 2547 2547
Size: 5543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 370 3562 370
Size: 3192 2351
FspI TGC'GCA
Cuts at: 3324 3324
Size: 5543
HaeII r_GCGC'y
Cuts at: 2235 2977 3225 4455 5226 2235
Size: 742 248 1230 771 2552
Fragments arranged by size:
2552 1230 771 742 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 657 689 819 851 1236 1268 1318
Size: 32 408 32 130 32 385 32 50
Cuts at: 1318 1350 1771 1803 3385 3415 3417 3447 217
Size: 32 421 32 1582 30 2 30 2313
Fragments arranged by size:
2313 1582 421 408 385 130 50 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1878 1986 2582 1878
Size: 108 596 4839
Fragments arranged by size:
4839 596 108
HindIII A'AGCT_T
Cuts at: 1159 1741 1159
Size: 582 4961
KasI G'GCGC_C
Cuts at: 2231 2973 3221 2231
Size: 742 248 4553
Fragments arranged by size:
4553 742 248
KpnI G_GTAC'C
Cuts at: 2563 2563
Size: 5543
MluI A'CGCG_T
Cuts at: 4976 4976
Size: 5543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 751 2090 4302 4486 521
Size: 230 1339 2212 184 1578
Fragments arranged by size:
2212 1578 1339 230 184
MscI TGG'CCA
Cuts at: 1667 3304 1667
Size: 1637 3906
MslI CAynn'nnrTG
Cuts at: 173 1315 1810 1972 3532 3659 173
Size: 1142 495 162 1560 127 2057
Fragments arranged by size:
2057 1560 1142 495 162 127
MspA1I CmG'CkG
Cuts at: 603 2023 2654 2968 3328 4112 4357 603
Size: 1420 631 314 360 784 245 1789
Fragments arranged by size:
1789 1420 784 631 360 314 245
NaeI GCC'GGC
Cuts at: 3724 3724
Size: 5543
NarI GG'CG_CC
Cuts at: 2232 2974 3222 2232
Size: 742 248 4553
Fragments arranged by size:
4553 742 248
NcoI C'CATG_G
Cuts at: 107 2142 107
Size: 2035 3508
NgoMIV G'CCGG_C
Cuts at: 3722 3722
Size: 5543
NspI r_CATG'y
Cuts at: 1984 2596 3096 4699 1984
Size: 612 500 1603 2828
Fragments arranged by size:
2828 1603 612 500
PasI CC'CwG_GG
Cuts at: 279 279
Size: 5543
PciI A'CATG_T
Cuts at: 4695 4695
Size: 5543
PflMI CCAn_nnn'nTGG
Cuts at: 1652 1652
Size: 5543
PfoI T'CCnGG_A
Cuts at: 661 2159 661
Size: 1498 4045
PmeI GTTT'AAAC
Cuts at: 2542 2542
Size: 5543
PmlI CAC'GTG
Cuts at: 1974 1974
Size: 5543
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 5511
PpuMI rG'GwC_Cy
Cuts at: 155 918 1360 155
Size: 763 442 4338
Fragments arranged by size:
4338 763 442
PstI C_TGCA'G
Cuts at: 2590 3275 2590
Size: 685 4858
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5543
PvuII CAG'CTG
Cuts at: 603 2023 2968 3328 603
Size: 1420 945 360 2818
Fragments arranged by size:
2818 1420 945 360
RsrII CG'GwC_CG
Cuts at: 3738 3738
Size: 5543
SacI G_AGCT'C
Cuts at: 512 2557 512
Size: 2045 3498
SalI G'TCGA_C
Cuts at: 2580 2580
Size: 5543
SapI GCTCTTCn'nnn_
Cuts at: 3566 3776 3566
Size: 210 5333
SbfI CC_TGCA'GG
Cuts at: 2590 2590
Size: 5543
ScaI AGT'ACT
Cuts at: 5060 5060
Size: 5543
SexAI A'CCwGG_T
Cuts at: 323 2519 323
Size: 2196 3347
SfcI C'TryA_G
Cuts at: 33 968 2463 2586 3271 4239 4430 33
Size: 935 1495 123 685 968 191 1146
Fragments arranged by size:
1495 1146 968 935 685 191 123
SfoI GGC'GCC
Cuts at: 2233 2975 3223 2233
Size: 742 248 4553
Fragments arranged by size:
4553 742 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5543
SgrAI Cr'CCGG_yG
Cuts at: 2012 2012
Size: 5543
SmaI CCC'GGG
Cuts at: 2565 2565
Size: 5543
SmlI C'TyrA_G
Cuts at: 385 433 836 1047 2698 3613 4327 4589 385
Size: 48 403 211 1651 915 714 262 1339
Fragments arranged by size:
1651 1339 915 714 403 262 211 48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5543
SphI G_CATG'C
Cuts at: 2596 3096 2596
Size: 500 5043
StuI AGG'CCT
Cuts at: 2150 2150
Size: 5543
StyI C'CwwG_G
Cuts at: 107 1109 1541 2142 2347 2677 3031 107
Size: 1002 432 601 205 330 354 2619
Fragments arranged by size:
2619 1002 601 432 354 330 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1739 1800 2133 3126 3259 3718 3927 1739
Size: 61 333 993 133 459 209 3355
Fragments arranged by size:
3355 993 459 333 209 133 61
TatI w'GTAC_w
Cuts at: 1847 2055 5058 1847
Size: 208 3003 2332
Fragments arranged by size:
3003 2332 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 311 343 1254 1286 5060 5092 311
Size: 32 911 32 3774 32 762
Fragments arranged by size:
3774 911 762 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2440 3340 2440
Size: 900 4643
XbaI T'CTAG_A
Cuts at: 216 1038 1870 2574 216
Size: 822 832 704 3185
Fragments arranged by size:
3185 832 822 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 3038
Size: 5543
XmaI C'CCGG_G
Cuts at: 2563 2563
Size: 5543
XmnI GAAnn'nnTTC
Cuts at: 290 290
Size: 5543
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I ClaI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SnaBI SrfI SspI SwaI XhoI ZraI