Restriction map for
pF1KB1380
(Circular) (Six-base) MAPSORT of: pF1KB1380.seq Check: 2925 from: 1 to: 5234
pF1KB1380 5234 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2272 2272
Size: 5234
Acc65I G'GTAC_C
Cuts at: 2250 2250
Size: 5234
AclI AA'CG_TT
Cuts at: 5074 5074
Size: 5234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 561 813 1233 1905 3059 3491 3844 4506
Size: 348 252 420 672 1154 432 353 662
Cuts at: 4506 213
Size: 941
Fragments arranged by size:
1154 941 672 662 432 420 353 348
252
AflIII A'CryG_T
Cuts at: 637 728 1066 2586 4386 4667 637
Size: 91 338 1520 1800 281 1204
Fragments arranged by size:
1800 1520 1204 338 281 91
AlwNI CAG_nnn'CTG
Cuts at: 1788 2539 3977 1788
Size: 751 1438 3045
Fragments arranged by size:
3045 1438 751
ApaI G_GGCC'C
Cuts at: 1512 1512
Size: 5234
ApaLI G'TGCA_C
Cuts at: 1873 4072 1873
Size: 2199 3035
ApoI r'AATT_y
Cuts at: 336 742 1341 1593 1845 2013 2238 4587 4843
Size: 406 599 252 252 168 225 2349 256
Cuts at: 4843 336
Size: 727
Fragments arranged by size:
2349 727 599 406 256 252 252 225
168
AseI AT'TA_AT
Cuts at: 20 20
Size: 5234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5234
AvaI C'yCGr_G
Cuts at: 2254 2254
Size: 5234
BamHI G'GATC_C
Cuts at: 2259 2259
Size: 5234
BanI G'GyrC_C
Cuts at: 2250 2664 2912 2947 2250
Size: 414 248 35 4537
Fragments arranged by size:
4537 414 248 35
BanII G_rGCy'C
Cuts at: 1512 2248 3278 1512
Size: 736 1030 3468
Fragments arranged by size:
3468 1030 736
BbeI G_GCGC'C
Cuts at: 2668 2916 2668
Size: 248 4986
BbsI GAAGACnn'nnnn_
Cuts at: 147 319 147
Size: 172 5062
BciVI GTATCCnnnnn_n'
Cuts at: 264 3132 4188 5234 264
Size: 2868 1056 1046 264
Fragments arranged by size:
2868 1056 1046 264
BclI T'GATC_A
Cuts at: 2754 2754
Size: 5234
BfrBI ATG'CAT
Cuts at: 2115 2115
Size: 5234
BglII A'GATC_T
Cuts at: 1179 2749 4786 1179
Size: 1570 2037 1627
Fragments arranged by size:
2037 1627 1570
BlpI GC'TnA_GC
Cuts at: 2346 2346
Size: 5234
Bme1580I G_kGCm'C
Cuts at: 1512 1877 2859 2952 4076 1512
Size: 365 982 93 1124 2670
Fragments arranged by size:
2670 1124 982 365 93
BmgBI CAC'GTC
Cuts at: 144 729 144
Size: 585 4649
BmrI ACTGGGnnnn_n'
Cuts at: 2446 2527 2862 5006 2446
Size: 81 335 2144 2674
Fragments arranged by size:
2674 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1604 3601 1604
Size: 1997 3237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 809 1649 1901 241
Size: 568 840 252 3574
Fragments arranged by size:
3574 840 568 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 2169 2410 3289 4003 4301 434
Size: 1735 241 879 714 298 1367
Fragments arranged by size:
1735 1367 879 714 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 452 1713 4938 452
Size: 1261 3225 748
Fragments arranged by size:
3225 1261 748
BsaAI yAC'GTr
Cuts at: 638 1067 2587 638
Size: 429 1520 3285
Fragments arranged by size:
3285 1520 429
BsaBI GATnn'nnATC
Cuts at: 6 602 2114 2748 4785 6
Size: 596 1512 634 2037 455
Fragments arranged by size:
2037 1512 634 596 455
BsaHI Gr'CG_yC
Cuts at: 1956 2665 2913 3615 5111 1956
Size: 709 248 702 1496 2079
Fragments arranged by size:
2079 1496 709 702 248
BsaWI w'CCGG_w
Cuts at: 2423 2944 3622 4033 4180 4454 2423
Size: 521 678 411 147 274 3203
Fragments arranged by size:
3203 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1162 1162
Size: 5234
BseYI C'CCAG_C
Cuts at: 171 926 1757 2659 3417 4082 4490 171
Size: 755 831 902 758 665 408 915
Fragments arranged by size:
915 902 831 758 755 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 1774
Size: 5234
BsiEI CG_ry'CG
Cuts at: 104 2776 2822 4052 104
Size: 2672 46 1230 1286
Fragments arranged by size:
2672 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1877 2248 3026 3216 4076 1877
Size: 371 778 190 860 3035
Fragments arranged by size:
3035 860 778 371 190
BsmI GAATG_Cn'
Cuts at: 259 904 1023 259
Size: 645 119 4470
Fragments arranged by size:
4470 645 119
BsmBI CGTCTCn'nnnn_
Cuts at: 1947 1947
Size: 5234
Bsp1286I G_dGCh'C
Cuts at: 1512 1877 2248 2859 2952 3026 3216 3278 4076
Size: 365 371 611 93 74 190 62 798
Cuts at: 4076 1512
Size: 2670
Fragments arranged by size:
2670 798 611 371 365 190 93 74
62
BspHI T'CATG_A
Cuts at: 3666 3666
Size: 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 245 2284 2498 2800 3181 245
Size: 2039 214 302 381 2298
Fragments arranged by size:
2298 2039 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3526 3580 5064 5229 50
Size: 3476 54 1484 165 55
Fragments arranged by size:
3476 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 134 564 3146 134
Size: 430 2582 2222
Fragments arranged by size:
2582 2222 430
BsrFI r'CCGG_y
Cuts at: 3232 3413 3232
Size: 181 5053
BsrGI T'GTAC_A
Cuts at: 2007 2007
Size: 5234
BssSI C'ACGA_G
Cuts at: 1260 1764 3505 4213 1260
Size: 504 1741 708 2281
Fragments arranged by size:
2281 1741 708 504
BstAPI GCAn_nnn'nTGC
Cuts at: 2611 2611
Size: 5234
BstBI TT'CG_AA
Cuts at: 3595 3595
Size: 5234
BstXI CCAn_nnnn'nTGG
Cuts at: 559 559
Size: 5234
BstYI r'GATC_y
Cuts at: 1 350 1179 1366 1431 2259 2726 2749 3084
Size: 349 829 187 65 828 467 23 335
Cuts at: 3084 3330 3636 3648 3734 4786 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 829 828 467 449 349 335 306
246 187 86 65 23 12
Bsu36I CC'TnA_GG
Cuts at: 286 286
Size: 5234
BtgI C'CryG_G
Cuts at: 1028 1028
Size: 5234
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1093 3184 3365 3528 1093
Size: 2091 181 163 2799
Fragments arranged by size:
2799 2091 181 163
BtsI GCAGTG_nn'
Cuts at: 2477 2477
Size: 5234
DraI TTT'AAA
Cuts at: 2233 2233
Size: 5234
DrdI GACnn_nn'nnGTC
Cuts at: 2940 4284 2940
Size: 1344 3890
EaeI y'GGCC_r
Cuts at: 2819 2993 3384 3411 4756 2819
Size: 174 391 27 1345 3297
Fragments arranged by size:
3297 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2819 2819
Size: 5234
EarI CTCTTCn'nnn_
Cuts at: 161 1237 1489 1562 1573 1741 1814 3257 3467
Size: 1076 252 73 11 168 73 1443 210
Cuts at: 3467 161
Size: 1928
Fragments arranged by size:
1928 1443 1076 252 210 168 73 73
11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3442 4184 4330 5044 3442
Size: 742 146 714 3632
Fragments arranged by size:
3632 742 714 146
EcoICRI GAG'CTC
Cuts at: 2246 2246
Size: 5234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 241 561 809 813 1233 1649 1901 1905
Size: 28 320 248 4 420 416 252 4
Cuts at: 1905 3059 3491 3844 4506 213
Size: 1154 432 353 662 941
Fragments arranged by size:
1154 941 662 432 420 416 353 320
252 248 28 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1509 2373 1509
Size: 864 4370
EcoRI G'AATT_C
Cuts at: 336 742 1845 2013 2238 336
Size: 406 1103 168 225 3332
Fragments arranged by size:
3332 1103 406 225 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3253 3253
Size: 5234
FspI TGC'GCA
Cuts at: 3015 3015
Size: 5234
HaeII r_GCGC'y
Cuts at: 2100 2668 2916 4146 4917 2100
Size: 568 248 1230 771 2417
Fragments arranged by size:
2417 1230 771 568 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 744 776 1068 1100 1416 1448 1488
Size: 32 377 32 292 32 316 32 40
Cuts at: 1488 1520 3076 3106 3108 3138 335
Size: 32 1556 30 2 30 2431
Fragments arranged by size:
2431 1556 377 316 292 40 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2273 2273
Size: 5234
KasI G'GCGC_C
Cuts at: 2664 2912 2664
Size: 248 4986
KpnI G_GTAC'C
Cuts at: 2254 2254
Size: 5234
MfeI C'AATT_G
Cuts at: 1728 1728
Size: 5234
MluI A'CGCG_T
Cuts at: 4667 4667
Size: 5234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1409 2196 3993 4177 1409
Size: 787 1797 184 2466
Fragments arranged by size:
2466 1797 787 184
MscI TGG'CCA
Cuts at: 2995 2995
Size: 5234
MslI CAynn'nnrTG
Cuts at: 638 972 1056 1140 1308 1392 1560 1644 1812
Size: 334 84 84 168 84 168 84 168
Cuts at: 1812 1896 2064 2148 2204 3223 3350 638
Size: 84 168 84 56 1019 127 2522
Fragments arranged by size:
2522 1019 334 168 168 168 168 127
84 84 84 84 84 84 56
MspA1I CmG'CkG
Cuts at: 1925 2345 2659 3019 3803 4048 1925
Size: 420 314 360 784 245 3111
Fragments arranged by size:
3111 784 420 360 314 245
NaeI GCC'GGC
Cuts at: 3415 3415
Size: 5234
NarI GG'CG_CC
Cuts at: 2665 2913 2665
Size: 248 4986
NcoI C'CATG_G
Cuts at: 1028 1028
Size: 5234
NdeI CA'TA_TG
Cuts at: 1196 1196
Size: 5234
NgoMIV G'CCGG_C
Cuts at: 3413 3413
Size: 5234
NsiI A_TGCA'T
Cuts at: 2117 2117
Size: 5234
NspI r_CATG'y
Cuts at: 2287 2787 4390 2287
Size: 500 1603 3131
Fragments arranged by size:
3131 1603 500
PasI CC'CwG_GG
Cuts at: 552 552
Size: 5234
PciI A'CATG_T
Cuts at: 4386 4386
Size: 5234
PmeI GTTT'AAAC
Cuts at: 2233 2233
Size: 5234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 226 258 784 816 3680 3712 226
Size: 32 526 32 2864 32 1748
Fragments arranged by size:
2864 1748 526 32 32 32
PsiI TTA'TAA
Cuts at: 887 887
Size: 5234
PspOMI G'GGCC_C
Cuts at: 1508 1508
Size: 5234
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 649 681 649
Size: 32 5202
PstI C_TGCA'G
Cuts at: 2281 2966 2281
Size: 685 4549
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5234
PvuII CAG'CTG
Cuts at: 1925 2659 3019 1925
Size: 734 360 4140
Fragments arranged by size:
4140 734 360
RsrII CG'GwC_CG
Cuts at: 3429 3429
Size: 5234
SacI G_AGCT'C
Cuts at: 2248 2248
Size: 5234
SalI G'TCGA_C
Cuts at: 2271 2271
Size: 5234
SapI GCTCTTCn'nnn_
Cuts at: 161 1237 1489 3257 3467 161
Size: 1076 252 1768 210 1928
Fragments arranged by size:
1928 1768 1076 252 210
SbfI CC_TGCA'GG
Cuts at: 2281 2281
Size: 5234
ScaI AGT'ACT
Cuts at: 4751 4751
Size: 5234
SexAI A'CCwGG_T
Cuts at: 442 442
Size: 5234
SfcI C'TryA_G
Cuts at: 33 2277 2962 3930 4121 33
Size: 2244 685 968 191 1146
Fragments arranged by size:
2244 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2666 2914 2666
Size: 248 4986
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5234
SmaI CCC'GGG
Cuts at: 2256 2256
Size: 5234
SmlI C'TyrA_G
Cuts at: 449 2184 2389 3304 4018 4280 449
Size: 1735 205 915 714 262 1403
Fragments arranged by size:
1735 1403 915 714 262 205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5234
SphI G_CATG'C
Cuts at: 2287 2787 2287
Size: 500 4734
StuI AGG'CCT
Cuts at: 910 2182 910
Size: 1272 3962
StyI C'CwwG_G
Cuts at: 1028 2368 2722 1028
Size: 1340 354 3540
Fragments arranged by size:
3540 1340 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2817 2950 3409 3618 2817
Size: 133 459 209 4433
Fragments arranged by size:
4433 459 209 133
TatI w'GTAC_w
Cuts at: 2007 4749 2007
Size: 2742 2492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1351 1383 4751 4783 1351
Size: 32 3368 32 1802
Fragments arranged by size:
3368 1802 32 32
Tth111I GACn'n_nGTC
Cuts at: 3031 3031
Size: 5234
XbaI T'CTAG_A
Cuts at: 2265 2265
Size: 5234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 5234
XmaI C'CCGG_G
Cuts at: 2254 2254
Size: 5234
XmnI GAAnn'nnTTC
Cuts at: 799 826 910 967 1051 1246 1918 2017 2039
Size: 27 84 57 84 195 672 99 22
Cuts at: 2039 799
Size: 3994
Fragments arranged by size:
3994 672 195 99 84 84 57 27
22
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PmeI PpiI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BcgI BglI BmtI
Bpu10I BsaXI BsiWI BspEI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NheI NotI NruI PacI PflMI PfoI PmlI PpuMI
PshAI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI