Restriction map for
pF1KB1338
(Circular) (Six-base) MAPSORT of: pF1KB1338.seq Check: 1541 from: 1 to: 4928
pF1KB1338 4928 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 483 483
Size: 4928
AatII G_ACGT'C
Cuts at: 727 727
Size: 4928
AccI GT'mk_AC
Cuts at: 1966 1966
Size: 4928
Acc65I G'GTAC_C
Cuts at: 1944 1944
Size: 4928
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 594 2753 3185 3538 4200 594
Size: 2159 432 353 662 1322
Fragments arranged by size:
2159 1322 662 432 353
AfeI AGC'GCT
Cuts at: 1620 1620
Size: 4928
AflIII A'CryG_T
Cuts at: 326 2280 4080 4361 326
Size: 1954 1800 281 893
Fragments arranged by size:
1954 1800 893 281
AhdI GACnn_n'nnGTC
Cuts at: 1062 1062
Size: 4928
AlwNI CAG_nnn'CTG
Cuts at: 1350 1854 2233 3671 1350
Size: 504 379 1438 2607
Fragments arranged by size:
2607 1438 504 379
ApaI G_GGCC'C
Cuts at: 211 1175 1197 211
Size: 964 22 3942
Fragments arranged by size:
3942 964 22
ApaLI G'TGCA_C
Cuts at: 148 3766 148
Size: 3618 1310
ApoI r'AATT_y
Cuts at: 1932 4281 4537 1932
Size: 2349 256 2323
Fragments arranged by size:
2349 2323 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4928
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 644 1496 1513 1948 644
Size: 852 17 435 3624
Fragments arranged by size:
3624 852 435 17
BamHI G'GATC_C
Cuts at: 1033 1953 1033
Size: 920 4008
BanI G'GyrC_C
Cuts at: 128 1279 1572 1728 1906 1944 2358 2606 2641
Size: 1151 293 156 178 38 414 248 35
Cuts at: 2641 128
Size: 2415
Fragments arranged by size:
2415 1151 414 293 248 178 156 38
35
BanII G_rGCy'C
Cuts at: 211 373 652 1142 1175 1197 1741 1873 1942
Size: 162 279 490 33 22 544 132 69
Cuts at: 1942 2972 211
Size: 1030 2167
Fragments arranged by size:
2167 1030 544 490 279 162 132 69
33 22
BbeI G_GCGC'C
Cuts at: 132 1732 2362 2610 132
Size: 1600 630 248 2450
Fragments arranged by size:
2450 1600 630 248
BbsI GAAGACnn'nnnn_
Cuts at: 1812 1812
Size: 4928
BbvCI CC'TCA_GC
Cuts at: 1533 1533
Size: 4928
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3882 4928 2826
Size: 1056 1046 2826
Fragments arranged by size:
2826 1056 1046
BclI T'GATC_A
Cuts at: 2448 2448
Size: 4928
BglI GCCn_nnn'nGGC
Cuts at: 623 1170 1712 623
Size: 547 542 3839
Fragments arranged by size:
3839 547 542
BglII A'GATC_T
Cuts at: 2443 4480 2443
Size: 2037 2891
BlpI GC'TnA_GC
Cuts at: 1774 1872 2040 1774
Size: 98 168 4662
Fragments arranged by size:
4662 168 98
Bme1580I G_kGCm'C
Cuts at: 152 211 1175 1197 2553 2646 3770 152
Size: 59 964 22 1356 93 1124 1310
Fragments arranged by size:
1356 1310 1124 964 93 59 22
BmrI ACTGGGnnnn_n'
Cuts at: 1150 1238 1403 2140 2221 2556 4700 1150
Size: 88 165 737 81 335 2144 1378
Fragments arranged by size:
2144 1378 737 335 165 88 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 4928
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 724 1276 1295 1360 595
Size: 129 552 19 65 4163
Fragments arranged by size:
4163 552 129 65 19
Bpu10I CC'TnA_GC
Cuts at: 1533 1533
Size: 4928
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 409 493 535 2104 2983 3697 3995 346
Size: 63 84 42 1569 879 714 298 1279
Fragments arranged by size:
1569 1279 879 714 298 84 63 42
BsaI GGTCTCn'nnnn_
Cuts at: 1070 1252 1318 1391 1618 4632 1070
Size: 182 66 73 227 3014 1366
Fragments arranged by size:
3014 1366 227 182 73 66
BsaAI yAC'GTr
Cuts at: 327 2281 327
Size: 1954 2974
BsaBI GATnn'nnATC
Cuts at: 6 2442 4479 6
Size: 2436 2037 455
Fragments arranged by size:
2436 2037 455
BsaHI Gr'CG_yC
Cuts at: 129 467 724 1729 2359 2607 3309 4805 129
Size: 338 257 1005 630 248 702 1496 252
Fragments arranged by size:
1496 1005 702 630 338 257 252 248
BsaWI w'CCGG_w
Cuts at: 2117 2638 3316 3727 3874 4148 2117
Size: 521 678 411 147 274 2897
Fragments arranged by size:
2897 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 625 655 685 781 811 142
Size: 30 453 30 30 96 30 4259
Fragments arranged by size:
4259 453 96 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 532 660 663 790 907 910 1119 1122 532
Size: 128 3 127 117 3 209 3 4338
Fragments arranged by size:
4338 209 128 127 117 3 3 3
BseYI C'CCAG_C
Cuts at: 603 1168 1222 1366 1402 1478 1701 2353 3111
Size: 565 54 144 36 76 223 652 758
Cuts at: 3111 3776 4184 603
Size: 665 408 1347
Fragments arranged by size:
1347 758 665 652 565 408 223 144
76 54 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 854
Size: 4928
BsiEI CG_ry'CG
Cuts at: 104 447 2470 2516 3746 104
Size: 343 2023 46 1230 1286
Fragments arranged by size:
2023 1286 1230 343 46
BsiHKAI G_wGCw'C
Cuts at: 152 1873 1942 2720 2910 3770 152
Size: 1721 69 778 190 860 1310
Fragments arranged by size:
1721 1310 860 778 190 69
BsmBI CGTCTCn'nnnn_
Cuts at: 1648 1648
Size: 4928
Bsp1286I G_dGCh'C
Cuts at: 152 211 373 652 1142 1175 1197 1741 1873
Size: 59 162 279 490 33 22 544 132
Cuts at: 1873 1942 2553 2646 2720 2910 2972 3770 152
Size: 69 611 93 74 190 62 798 1310
Fragments arranged by size:
1310 798 611 544 490 279 190 162
132 93 74 69 62 59 33 22
BspHI T'CATG_A
Cuts at: 3360 3360
Size: 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 365 483 960 1978 2192 2494 2875 365
Size: 118 477 1018 214 302 381 2418
Fragments arranged by size:
2418 1018 477 381 302 214 118
BsrBI CCG'CTC
Cuts at: 50 378 522 641 1773 3220 3274 4758 4923
Size: 328 144 119 1132 1447 54 1484 165
Cuts at: 4923 50
Size: 55
Fragments arranged by size:
1484 1447 1132 328 165 144 119 55
54
BsrDI GCAATG_nn'
Cuts at: 201 343 2840 201
Size: 142 2497 2289
Fragments arranged by size:
2497 2289 142
BsrFI r'CCGG_y
Cuts at: 1687 1695 1903 2926 3107 1687
Size: 8 208 1023 181 3508
Fragments arranged by size:
3508 1023 208 181 8
BsrGI T'GTAC_A
Cuts at: 329 329
Size: 4928
BssSI C'ACGA_G
Cuts at: 3199 3907 3199
Size: 708 4220
BstAPI GCAn_nnn'nTGC
Cuts at: 1350 1854 2305 1350
Size: 504 451 3973
Fragments arranged by size:
3973 504 451
BstBI TT'CG_AA
Cuts at: 3289 3289
Size: 4928
BstYI r'GATC_y
Cuts at: 1 1033 1110 1953 2420 2443 2778 3024 3330
Size: 1032 77 843 467 23 335 246 306
Cuts at: 3330 3342 3428 4480 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1032 843 467 449 335 306 246
86 77 23 12
Bsu36I CC'TnA_GG
Cuts at: 1077 1077
Size: 4928
BtgI C'CryG_G
Cuts at: 821 842 991 1882 821
Size: 21 149 891 3867
Fragments arranged by size:
3867 891 149 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2878 3059 3222 2878
Size: 181 163 4584
Fragments arranged by size:
4584 181 163
BtsI GCAGTG_nn'
Cuts at: 600 975 2171 600
Size: 375 1196 3357
Fragments arranged by size:
3357 1196 375
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4928
DraIII CAC_nnn'GTG
Cuts at: 1276 1276
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 466 1515 1564 2634 3978 466
Size: 1049 49 1070 1344 1416
Fragments arranged by size:
1416 1344 1070 1049 49
EaeI y'GGCC_r
Cuts at: 824 2513 2687 3078 3105 4450 824
Size: 1689 174 391 27 1345 1302
Fragments arranged by size:
1689 1345 1302 391 174 27
EagI C'GGCC_G
Cuts at: 2513 2513
Size: 4928
EarI CTCTTCn'nnn_
Cuts at: 681 728 875 2951 3161 681
Size: 47 147 2076 210 2448
Fragments arranged by size:
2448 2076 210 147 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3136 3878 4024 4738 3136
Size: 742 146 714 3326
Fragments arranged by size:
3326 742 714 146
EcoICRI GAG'CTC
Cuts at: 1871 1940 1871
Size: 69 4859
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 594 595 724 1276 1295 1360 2753 3185 3538
Size: 1 129 552 19 65 1393 432 353
Cuts at: 3538 4200 594
Size: 662 1322
Fragments arranged by size:
1393 1322 662 552 432 353 129 65
19 1
EcoNI CCTnn'n_nnAGG
Cuts at: 508 706 1146 508
Size: 198 440 4290
Fragments arranged by size:
4290 440 198
EcoO109I rG'GnC_Cy
Cuts at: 207 208 797 1045 1151 1172 1194 1347 1528
Size: 1 589 248 106 21 22 153 181
Cuts at: 1528 2067 207
Size: 539 3068
Fragments arranged by size:
3068 589 539 248 181 153 106 22
21 1
EcoRI G'AATT_C
Cuts at: 1932 1932
Size: 4928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 4928
FspI TGC'GCA
Cuts at: 1684 2709 1684
Size: 1025 3903
FspAI rTGC'GCAy
Cuts at: 1684 1684
Size: 4928
HaeII r_GCGC'y
Cuts at: 132 1622 1702 1732 1757 2362 2610 3840 4611
Size: 1490 80 30 25 605 248 1230 771
Cuts at: 4611 132
Size: 449
Fragments arranged by size:
1490 1230 771 605 449 248 80 30
25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 781 813 1048 1080 2770 2800 2802 2832 781
Size: 32 235 32 1690 30 2 30 2877
Fragments arranged by size:
2877 1690 235 32 32 30 30 2
HincII GTy'rAC
Cuts at: 471 1551 1967 471
Size: 1080 416 3432
Fragments arranged by size:
3432 1080 416
KasI G'GCGC_C
Cuts at: 128 1728 2358 2606 128
Size: 1600 630 248 2450
Fragments arranged by size:
2450 1600 630 248
KpnI G_GTAC'C
Cuts at: 1948 1948
Size: 4928
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 1289 3687 3871 722
Size: 567 2398 184 1779
Fragments arranged by size:
2398 1779 567 184
MscI TGG'CCA
Cuts at: 826 2689 826
Size: 1863 3065
MslI CAynn'nnrTG
Cuts at: 280 2917 3044 280
Size: 2637 127 2164
Fragments arranged by size:
2637 2164 127
MspA1I CmG'CkG
Cuts at: 308 603 747 753 858 1789 1854 1884 2039
Size: 295 144 6 105 931 65 30 155
Cuts at: 2039 2353 2713 3497 3742 308
Size: 314 360 784 245 1494
Fragments arranged by size:
1494 931 784 360 314 295 245 155
144 105 65 30 6
NaeI GCC'GGC
Cuts at: 1697 3109 1697
Size: 1412 3516
NarI GG'CG_CC
Cuts at: 129 1729 2359 2607 129
Size: 1600 630 248 2450
Fragments arranged by size:
2450 1600 630 248
NcoI C'CATG_G
Cuts at: 821 991 821
Size: 170 4758
NgoMIV G'CCGG_C
Cuts at: 1695 3107 1695
Size: 1412 3516
NspI r_CATG'y
Cuts at: 285 1981 2481 4084 285
Size: 1696 500 1603 1129
Fragments arranged by size:
1696 1603 1129 500
PasI CC'CwG_GG
Cuts at: 203 1106 1142 1190 1734 203
Size: 903 36 48 544 3397
Fragments arranged by size:
3397 903 544 48 36
PciI A'CATG_T
Cuts at: 4080 4080
Size: 4928
PfoI T'CCnGG_A
Cuts at: 926 926
Size: 4928
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PmlI CAC'GTG
Cuts at: 327 327
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4896
PpuMI rG'GwC_Cy
Cuts at: 797 1151 1528 797
Size: 354 377 4197
Fragments arranged by size:
4197 377 354
PspOMI G'GGCC_C
Cuts at: 207 1171 1193 207
Size: 964 22 3942
Fragments arranged by size:
3942 964 22
PstI C_TGCA'G
Cuts at: 746 752 1056 1975 2660 746
Size: 6 304 919 685 3014
Fragments arranged by size:
3014 919 685 304 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 603 747 753 1789 2353 2713 603
Size: 144 6 1036 564 360 2818
Fragments arranged by size:
2818 1036 564 360 144 6
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 1873 1942 1873
Size: 69 4859
SacII CC_GC'GG
Cuts at: 1885 1885
Size: 4928
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SanDI GG'GwC_CC
Cuts at: 1528 1528
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 2951 3161 2951
Size: 210 4718
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4928
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SexAI A'CCwGG_T
Cuts at: 1272 1272
Size: 4928
SfcI C'TryA_G
Cuts at: 33 633 742 748 1052 1749 1971 2656 3624
Size: 600 109 6 304 697 222 685 968
Cuts at: 3624 3815 33
Size: 191 1146
Fragments arranged by size:
1146 968 697 685 600 304 222 191
109 6
SfoI GGC'GCC
Cuts at: 130 1730 2360 2608 130
Size: 1600 630 248 2450
Fragments arranged by size:
2450 1600 630 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SgrAI Cr'CCGG_yG
Cuts at: 1695 1695
Size: 4928
SmaI CCC'GGG
Cuts at: 646 1950 646
Size: 1304 3624
SmlI C'TyrA_G
Cuts at: 361 424 508 550 2083 2998 3712 3974 361
Size: 63 84 42 1533 915 714 262 1315
Fragments arranged by size:
1533 1315 915 714 262 84 63 42
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 285 1981 2481 285
Size: 1696 500 2732
Fragments arranged by size:
2732 1696 500
StyI C'CwwG_G
Cuts at: 557 821 991 1240 1328 1544 2062 2416 557
Size: 264 170 249 88 216 518 354 3069
Fragments arranged by size:
3069 518 354 264 249 216 170 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2511 2644 3103 3312 2511
Size: 133 459 209 4127
Fragments arranged by size:
4127 459 209 133
TatI w'GTAC_w
Cuts at: 329 437 4443 329
Size: 108 4006 814
Fragments arranged by size:
4006 814 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 4445 4477 142
Size: 32 4271 32 593
Fragments arranged by size:
4271 593 32 32
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4928
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 564 930 1335 1374 2423 564
Size: 366 405 39 1049 3069
Fragments arranged by size:
3069 1049 405 366 39
XmaI C'CCGG_G
Cuts at: 644 1948 644
Size: 1304 3624
XmnI GAAnn'nnTTC
Cuts at: 1600 1600
Size: 4928
ZraI GAC'GTC
Cuts at: 725 725
Size: 4928
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AleI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BspEI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PshAI PsiI PspXI PsrI SfiI SnaBI SrfI
SspI StuI SwaI XhoI