Restriction map for
pF1KB1325
(Circular) (Six-base) MAPSORT of: pF1KB1325.seq Check: 8740 from: 1 to: 5375
pF1KB1325 5375 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 186 723 186
Size: 537 4838
AccI GT'mk_AC
Cuts at: 115 599 2413 115
Size: 484 1814 3077
Fragments arranged by size:
3077 1814 484
Acc65I G'GTAC_C
Cuts at: 2391 2391
Size: 5375
AclI AA'CG_TT
Cuts at: 5215 5215
Size: 5375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 489 1525 3200 3632 3985 4647 420
Size: 69 1036 1675 432 353 662 1148
Fragments arranged by size:
1675 1148 1036 662 432 353 69
AfeI AGC'GCT
Cuts at: 1285 1285
Size: 5375
AflIII A'CryG_T
Cuts at: 2727 4527 4808 2727
Size: 1800 281 3294
Fragments arranged by size:
3294 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 478 2680 4118 478
Size: 2202 1438 1735
Fragments arranged by size:
2202 1735 1438
ApaI G_GGCC'C
Cuts at: 1405 1405
Size: 5375
ApaLI G'TGCA_C
Cuts at: 4213 4213
Size: 5375
ApoI r'AATT_y
Cuts at: 2379 4728 4984 2379
Size: 2349 256 2770
Fragments arranged by size:
2770 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5375
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
AvaI C'yCGr_G
Cuts at: 676 849 2395 676
Size: 173 1546 3656
Fragments arranged by size:
3656 1546 173
BamHI G'GATC_C
Cuts at: 1593 1802 2400 1593
Size: 209 598 4568
Fragments arranged by size:
4568 598 209
BanI G'GyrC_C
Cuts at: 1086 1812 2391 2805 3053 3088 1086
Size: 726 579 414 248 35 3373
Fragments arranged by size:
3373 726 579 414 248 35
BanII G_rGCy'C
Cuts at: 400 641 1046 1405 2227 2389 3419 400
Size: 241 405 359 822 162 1030 2356
Fragments arranged by size:
2356 1030 822 405 359 241 162
BbeI G_GCGC'C
Cuts at: 2809 3057 2809
Size: 248 5127
BbsI GAAGACnn'nnnn_
Cuts at: 1336 1342 1336
Size: 6 5369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1848 1882 1848
Size: 34 5341
BciVI GTATCCnnnnn_n'
Cuts at: 1567 3273 4329 5375 1567
Size: 1706 1056 1046 1567
Fragments arranged by size:
1706 1567 1056 1046
BclI T'GATC_A
Cuts at: 1902 2895 1902
Size: 993 4382
BglI GCCn_nnn'nGGC
Cuts at: 310 2098 2330 310
Size: 1788 232 3355
Fragments arranged by size:
3355 1788 232
BglII A'GATC_T
Cuts at: 977 1190 2890 4927 977
Size: 213 1700 2037 1425
Fragments arranged by size:
2037 1700 1425 213
BlpI GC'TnA_GC
Cuts at: 2132 2487 2132
Size: 355 5020
Bme1580I G_kGCm'C
Cuts at: 1405 3000 3093 4217 1405
Size: 1595 93 1124 2563
Fragments arranged by size:
2563 1595 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 328 466 2121 2587 2668 3003 5147 328
Size: 138 1655 466 81 335 2144 556
Fragments arranged by size:
2144 1655 556 466 335 138 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1209 3742 1209
Size: 2533 2842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 1486
Size: 5375
Bpu10I CC'TnA_GC
Cuts at: 358 640 1495 1705 358
Size: 282 855 210 4028
Fragments arranged by size:
4028 855 282 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 1204 2068 2551 3430 4144 4442 414
Size: 790 864 483 879 714 298 1347
Fragments arranged by size:
1347 879 864 790 714 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 616 2086 5079 616
Size: 1470 2993 912
Fragments arranged by size:
2993 1470 912
BsaAI yAC'GTr
Cuts at: 2728 2728
Size: 5375
BsaBI GATnn'nnATC
Cuts at: 6 2889 4926 6
Size: 2883 2037 455
Fragments arranged by size:
2883 2037 455
BsaHI Gr'CG_yC
Cuts at: 1295 2806 3054 3756 5252 1295
Size: 1511 248 702 1496 1418
Fragments arranged by size:
1511 1496 1418 702 248
BsaWI w'CCGG_w
Cuts at: 895 2564 3085 3763 4174 4321 4595 895
Size: 1669 521 678 411 147 274 1675
Fragments arranged by size:
1675 1669 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 148 178 1813 1843 148
Size: 30 1635 30 3680
Fragments arranged by size:
3680 1635 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 538 649 964 1054 1821 157
Size: 381 111 315 90 767 3711
Fragments arranged by size:
3711 767 381 315 111 90
BseYI C'CCAG_C
Cuts at: 233 494 921 933 2800 3558 4223 4631 233
Size: 261 427 12 1867 758 665 408 977
Fragments arranged by size:
1867 977 758 665 427 408 261 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 224
Size: 5375
BsiEI CG_ry'CG
Cuts at: 104 1799 2917 2963 4193 104
Size: 1695 1118 46 1230 1286
Fragments arranged by size:
1695 1286 1230 1118 46
BsiHKAI G_wGCw'C
Cuts at: 400 641 1046 1501 2138 2206 2227 2389 3167
Size: 241 405 455 637 68 21 162 778
Cuts at: 3167 3357 4217 400
Size: 190 860 1558
Fragments arranged by size:
1558 860 778 637 455 405 241 190
162 68 21
BsmBI CGTCTCn'nnnn_
Cuts at: 985 985
Size: 5375
Bsp1286I G_dGCh'C
Cuts at: 400 641 1046 1405 1501 2138 2206 2227 2389
Size: 241 405 359 96 637 68 21 162
Cuts at: 2389 3000 3093 3167 3357 3419 4217 400
Size: 611 93 74 190 62 798 1558
Fragments arranged by size:
1558 798 637 611 405 359 241 190
162 96 93 74 68 62 21
BspHI T'CATG_A
Cuts at: 3807 3807
Size: 5375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 186 723 2425 2639 2941 3322 186
Size: 537 1702 214 302 381 2239
Fragments arranged by size:
2239 1702 537 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3667 3721 5205 5370 50
Size: 3617 54 1484 165 55
Fragments arranged by size:
3617 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3287 3287
Size: 5375
BsrFI r'CCGG_y
Cuts at: 2031 3373 3554 2031
Size: 1342 181 3852
Fragments arranged by size:
3852 1342 181
BssSI C'ACGA_G
Cuts at: 3646 4354 3646
Size: 708 4667
BstAPI GCAn_nnn'nTGC
Cuts at: 2752 2752
Size: 5375
BstBI TT'CG_AA
Cuts at: 3736 3736
Size: 5375
BstEII G'GTnAC_C
Cuts at: 738 738
Size: 5375
BstXI CCAn_nnnn'nTGG
Cuts at: 1605 1605
Size: 5375
BstYI r'GATC_y
Cuts at: 1 340 977 1190 1593 1802 2400 2867 2890
Size: 339 637 213 403 209 598 467 23
Cuts at: 2890 3225 3471 3777 3789 3875 4927 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 637 598 467 449 403 339 335
306 246 213 209 86 23 12
BstZ17I GTA'TAC
Cuts at: 600 600
Size: 5375
BtgI C'CryG_G
Cuts at: 401 560 1133 1748 1942 1961 401
Size: 159 573 615 194 19 3815
Fragments arranged by size:
3815 615 573 194 159 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2049 3325 3506 3669 2049
Size: 1276 181 163 3755
Fragments arranged by size:
3755 1276 181 163
BtsI GCAGTG_nn'
Cuts at: 1628 1691 2618 1628
Size: 63 927 4385
Fragments arranged by size:
4385 927 63
DraI TTT'AAA
Cuts at: 2374 2374
Size: 5375
DraIII CAC_nnn'GTG
Cuts at: 1059 1059
Size: 5375
DrdI GACnn_nn'nnGTC
Cuts at: 3081 4425 3081
Size: 1344 4031
EaeI y'GGCC_r
Cuts at: 222 483 563 1001 1796 1939 2960 3134 3525
Size: 261 80 438 795 143 1021 174 391
Cuts at: 3525 3552 4897 222
Size: 27 1345 700
Fragments arranged by size:
1345 1021 795 700 438 391 261 174
143 80 27
EagI C'GGCC_G
Cuts at: 1796 2960 1796
Size: 1164 4211
EarI CTCTTCn'nnn_
Cuts at: 2179 3398 3608 2179
Size: 1219 210 3946
Fragments arranged by size:
3946 1219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3583 4325 4471 5185 3583
Size: 742 146 714 3773
Fragments arranged by size:
3773 742 714 146
EcoICRI GAG'CTC
Cuts at: 398 639 1044 2225 2387 398
Size: 241 405 1181 162 3386
Fragments arranged by size:
3386 1181 405 241 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 489 1486 1525 3200 3632 3985 4647 420
Size: 69 997 39 1675 432 353 662 1148
Fragments arranged by size:
1675 1148 997 662 432 353 69 39
EcoO109I rG'GnC_Cy
Cuts at: 147 500 697 1402 2514 147
Size: 353 197 705 1112 3008
Fragments arranged by size:
3008 1112 705 353 197
EcoRI G'AATT_C
Cuts at: 2379 2379
Size: 5375
EcoRV GAT'ATC
Cuts at: 901 901
Size: 5375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 362 732 3394 362
Size: 370 2662 2343
Fragments arranged by size:
2662 2343 370
FspI TGC'GCA
Cuts at: 3156 3156
Size: 5375
HaeII r_GCGC'y
Cuts at: 1032 1287 2809 3057 4287 5058 1032
Size: 255 1522 248 1230 771 1349
Fragments arranged by size:
1522 1349 1230 771 255 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 472 504 885 917 1182 1211 1214
Size: 32 292 32 381 32 265 29 3
Cuts at: 1214 1243 3217 3247 3249 3279 148
Size: 29 1974 30 2 30 2244
Fragments arranged by size:
2244 1974 381 292 265 32 32 32
30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 116 2414 116
Size: 2298 3077
HindIII A'AGCT_T
Cuts at: 365 365
Size: 5375
KasI G'GCGC_C
Cuts at: 2805 3053 2805
Size: 248 5127
KpnI G_GTAC'C
Cuts at: 2395 2395
Size: 5375
MluI A'CGCG_T
Cuts at: 4808 4808
Size: 5375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 827 4134 4318 827
Size: 3307 184 1884
Fragments arranged by size:
3307 1884 184
MscI TGG'CCA
Cuts at: 224 485 1003 3136 224
Size: 261 518 2133 2463
Fragments arranged by size:
2463 2133 518 261
MslI CAynn'nnrTG
Cuts at: 3364 3491 3364
Size: 127 5248
MspA1I CmG'CkG
Cuts at: 921 2321 2486 2800 3160 3944 4189 921
Size: 1400 165 314 360 784 245 2107
Fragments arranged by size:
2107 1400 784 360 314 245 165
NaeI GCC'GGC
Cuts at: 3556 3556
Size: 5375
NarI GG'CG_CC
Cuts at: 2806 3054 2806
Size: 248 5127
NcoI C'CATG_G
Cuts at: 401 1748 1942 401
Size: 1347 194 3834
Fragments arranged by size:
3834 1347 194
NgoMIV G'CCGG_C
Cuts at: 3554 3554
Size: 5375
NspI r_CATG'y
Cuts at: 2428 2928 4531 2428
Size: 500 1603 3272
Fragments arranged by size:
3272 1603 500
PasI CC'CwG_GG
Cuts at: 701 2052 2151 701
Size: 1351 99 3925
Fragments arranged by size:
3925 1351 99
PciI A'CATG_T
Cuts at: 4527 4527
Size: 5375
PflMI CCAn_nnn'nTGG
Cuts at: 1100 1100
Size: 5375
PfoI T'CCnGG_A
Cuts at: 719 1463 719
Size: 744 4631
PmeI GTTT'AAAC
Cuts at: 2374 2374
Size: 5375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 3821
Size: 32 5343
PpuMI rG'GwC_Cy
Cuts at: 147 500 697 147
Size: 353 197 4825
Fragments arranged by size:
4825 353 197
PspOMI G'GGCC_C
Cuts at: 1401 1401
Size: 5375
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1747 1779 1747
Size: 32 5343
PstI C_TGCA'G
Cuts at: 197 734 1079 1635 1811 2422 3107 197
Size: 537 345 556 176 611 685 2465
Fragments arranged by size:
2465 685 611 556 537 345 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5375
PvuII CAG'CTG
Cuts at: 921 2800 3160 921
Size: 1879 360 3136
Fragments arranged by size:
3136 1879 360
RsrII CG'GwC_CG
Cuts at: 3570 3570
Size: 5375
SacI G_AGCT'C
Cuts at: 400 641 1046 2227 2389 400
Size: 241 405 1181 162 3386
Fragments arranged by size:
3386 1181 405 241 162
SalI G'TCGA_C
Cuts at: 114 2412 114
Size: 2298 3077
SapI GCTCTTCn'nnn_
Cuts at: 2179 3398 3608 2179
Size: 1219 210 3946
Fragments arranged by size:
3946 1219 210
SbfI CC_TGCA'GG
Cuts at: 197 734 1811 2422 197
Size: 537 1077 611 3150
Fragments arranged by size:
3150 1077 611 537
ScaI AGT'ACT
Cuts at: 4892 4892
Size: 5375
SexAI A'CCwGG_T
Cuts at: 149 1250 149
Size: 1101 4274
SfcI C'TryA_G
Cuts at: 33 193 730 1075 1631 1807 2013 2418 3103
Size: 160 537 345 556 176 206 405 685
Cuts at: 3103 4071 4262 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 556 537 405 345 206
191 176 160
SfoI GGC'GCC
Cuts at: 2807 3055 2807
Size: 248 5127
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5375
SmaI CCC'GGG
Cuts at: 2397 2397
Size: 5375
SmlI C'TyrA_G
Cuts at: 393 849 1183 2083 2530 3445 4159 4421 393
Size: 456 334 900 447 915 714 262 1347
Fragments arranged by size:
1347 915 900 714 456 447 334 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5375
SphI G_CATG'C
Cuts at: 2428 2928 2428
Size: 500 4875
StuI AGG'CCT
Cuts at: 1843 1969 2237 1843
Size: 126 268 4981
Fragments arranged by size:
4981 268 126
StyI C'CwwG_G
Cuts at: 401 707 1082 1538 1598 1748 1838 1942 2297
Size: 306 375 456 60 150 90 104 355
Cuts at: 2297 2509 2863 401
Size: 212 354 2913
Fragments arranged by size:
2913 456 375 355 354 306 212 150
104 90 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1851 1999 2103 2352 2958 3091 3550 3759 1851
Size: 148 104 249 606 133 459 209 3467
Fragments arranged by size:
3467 606 459 249 209 148 133 104
TatI w'GTAC_w
Cuts at: 2003 4890 2003
Size: 2887 2488
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 1620 1652 4892 4924 308
Size: 32 1280 32 3240 32 759
Fragments arranged by size:
3240 1280 759 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3172 3172
Size: 5375
XbaI T'CTAG_A
Cuts at: 2406 2406
Size: 5375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 654 1587 2870 654
Size: 933 1283 3159
Fragments arranged by size:
3159 1283 933
XhoI C'TCGA_G
Cuts at: 849 849
Size: 5375
XmaI C'CCGG_G
Cuts at: 2395 2395
Size: 5375
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII Bsu36I ClaI CspCI EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI