Restriction map for
pF1KB1226
(Circular) (Six-base) MAPSORT of: pF1KB1226.seq Check: 9570 from: 1 to: 6779
pF1KB1226 6779 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1491 1491
Size: 6779
AccI GT'mk_AC
Cuts at: 3817 3817
Size: 6779
Acc65I G'GTAC_C
Cuts at: 2975 3795 2975
Size: 820 5959
AclI AA'CG_TT
Cuts at: 6619 6619
Size: 6779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 2224 2977 4604 5036 5389 6051 397
Size: 1827 753 1627 432 353 662 1125
Fragments arranged by size:
1827 1627 1125 753 662 432 353
AflIII A'CryG_T
Cuts at: 1844 2063 4131 5931 6212 1844
Size: 219 2068 1800 281 2411
Fragments arranged by size:
2411 2068 1800 281 219
AgeI A'CCGG_T
Cuts at: 2033 2033
Size: 6779
AleI CACnn'nnGTG
Cuts at: 700 3508 700
Size: 2808 3971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 718 718
Size: 6779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1121 1153 1121
Size: 32 6747
AlwNI CAG_nnn'CTG
Cuts at: 531 874 1337 1624 1680 2220 2238 2955 3393
Size: 343 463 287 56 540 18 717 438
Cuts at: 3393 3712 4084 5522 531
Size: 319 372 1438 1788
Fragments arranged by size:
1788 1438 717 540 463 438 372 343
319 287 56 18
ApaI G_GGCC'C
Cuts at: 143 143
Size: 6779
ApaLI G'TGCA_C
Cuts at: 5617 5617
Size: 6779
ApoI r'AATT_y
Cuts at: 1915 3783 6132 6388 1915
Size: 1868 2349 256 2306
Fragments arranged by size:
2349 2306 1868 256
AseI AT'TA_AT
Cuts at: 20 3269 20
Size: 3249 3530
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6779
AvaI C'yCGr_G
Cuts at: 2533 2778 3799 2533
Size: 245 1021 5513
Fragments arranged by size:
5513 1021 245
AvrII C'CTAG_G
Cuts at: 393 393
Size: 6779
BamHI G'GATC_C
Cuts at: 3583 3804 3583
Size: 221 6558
BanI G'GyrC_C
Cuts at: 1020 2614 2975 3795 4209 4457 4492 1020
Size: 1594 361 820 414 248 35 3307
Fragments arranged by size:
3307 1594 820 414 361 248 35
BanII G_rGCy'C
Cuts at: 143 428 794 1073 2869 3029 3793 4823 143
Size: 285 366 279 1796 160 764 1030 2099
Fragments arranged by size:
2099 1796 1030 764 366 285 279 160
BbeI G_GCGC'C
Cuts at: 1024 4213 4461 1024
Size: 3189 248 3342
Fragments arranged by size:
3342 3189 248
BbsI GAAGACnn'nnnn_
Cuts at: 474 2060 2156 3002 3092 3766 474
Size: 1586 96 846 90 674 3487
Fragments arranged by size:
3487 1586 846 674 96 90
BbvCI CC'TCA_GC
Cuts at: 514 1301 2759 514
Size: 787 1458 4534
Fragments arranged by size:
4534 1458 787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3540 3574 90
Size: 34 3416 34 3295
Fragments arranged by size:
3416 3295 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4677 5733 6779 4677
Size: 1056 1046 4677
Fragments arranged by size:
4677 1056 1046
BclI T'GATC_A
Cuts at: 2588 3296 4299 2588
Size: 708 1003 5068
Fragments arranged by size:
5068 1003 708
BfrBI ATG'CAT
Cuts at: 1788 1788
Size: 6779
BglI GCCn_nnn'nGGC
Cuts at: 730 730
Size: 6779
BglII A'GATC_T
Cuts at: 4294 6331 4294
Size: 2037 4742
BlpI GC'TnA_GC
Cuts at: 175 3891 175
Size: 3716 3063
Bme1580I G_kGCm'C
Cuts at: 143 324 4404 4497 5621 143
Size: 181 4080 93 1124 1301
Fragments arranged by size:
4080 1301 1124 181 93
BmgBI CAC'GTC
Cuts at: 246 1083 1845 2066 246
Size: 837 762 221 4959
Fragments arranged by size:
4959 837 762 221
BmrI ACTGGGnnnn_n'
Cuts at: 497 578 2382 3465 3991 4072 4407 6551 497
Size: 81 1804 1083 526 81 335 2144 725
Fragments arranged by size:
2144 1804 1083 725 526 335 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2937 5146 2937
Size: 2209 4570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 909 1693 1840 1855 2405 2668 662
Size: 247 784 147 15 550 263 4773
Fragments arranged by size:
4773 784 550 263 247 147 15
Bpu10I CC'TnA_GC
Cuts at: 514 1301 1681 2759 2863 514
Size: 787 380 1078 104 4430
Fragments arranged by size:
4430 1078 787 380 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 1397 3145 3476 3955 4834 5548 5846 1228
Size: 169 1748 331 479 879 714 298 2161
Fragments arranged by size:
2161 1748 879 714 479 331 298 169
BsaI GGTCTCn'nnnn_
Cuts at: 927 6483 927
Size: 5556 1223
BsaAI yAC'GTr
Cuts at: 3124 4132 3124
Size: 1008 5771
BsaBI GATnn'nnATC
Cuts at: 6 1351 2488 2557 2965 4293 6330 6
Size: 1345 1137 69 408 1328 2037 455
Fragments arranged by size:
2037 1345 1328 1137 455 408 69
BsaHI Gr'CG_yC
Cuts at: 1021 4210 4458 5160 6656 1021
Size: 3189 248 702 1496 1144
Fragments arranged by size:
3189 1496 1144 702 248
BsaWI w'CCGG_w
Cuts at: 2033 2906 3968 4489 5167 5578 5725 5999 2033
Size: 873 1062 521 678 411 147 274 2813
Fragments arranged by size:
2813 1062 873 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 536 566 907 937 1121 1151 2893 2923 536
Size: 30 341 30 184 30 1742 30 4392
Fragments arranged by size:
4392 1742 341 184 30 30 30 30
BseYI C'CCAG_C
Cuts at: 135 4204 4962 5627 6035 135
Size: 4069 758 665 408 879
Fragments arranged by size:
4069 879 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 1519 2233 2341 2620 2959 438
Size: 1081 714 108 279 339 4258
Fragments arranged by size:
4258 1081 714 339 279 108
BsiEI CG_ry'CG
Cuts at: 104 419 4321 4367 5597 104
Size: 315 3902 46 1230 1286
Fragments arranged by size:
3902 1286 1230 315 46
BsiHKAI G_wGCw'C
Cuts at: 428 1256 1760 3793 4571 4761 5621 428
Size: 828 504 2033 778 190 860 1586
Fragments arranged by size:
2033 1586 860 828 778 504 190
BsmI GAATG_Cn'
Cuts at: 2843 2843
Size: 6779
Bsp1286I G_dGCh'C
Cuts at: 143 324 428 794 1073 1256 1760 2869 3029
Size: 181 104 366 279 183 504 1109 160
Cuts at: 3029 3793 4404 4497 4571 4761 4823 5621 143
Size: 764 611 93 74 190 62 798 1301
Fragments arranged by size:
1301 1109 798 764 611 504 366 279
190 183 181 160 104 93 74 62
BspHI T'CATG_A
Cuts at: 5211 5211
Size: 6779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1491 3829 4043 4345 4726 1491
Size: 2338 214 302 381 3544
Fragments arranged by size:
3544 2338 381 302 214
BsrBI CCG'CTC
Cuts at: 50 179 3189 5071 5125 6609 6774 50
Size: 129 3010 1882 54 1484 165 55
Fragments arranged by size:
3010 1882 1484 165 129 55 54
BsrDI GCAATG_nn'
Cuts at: 864 2289 3590 4691 864
Size: 1425 1301 1101 2952
Fragments arranged by size:
2952 1425 1301 1101
BsrFI r'CCGG_y
Cuts at: 189 2033 3625 3674 4777 4958 189
Size: 1844 1592 49 1103 181 2010
Fragments arranged by size:
2010 1844 1592 1103 181 49
BsrGI T'GTAC_A
Cuts at: 281 1602 2006 281
Size: 1321 404 5054
Fragments arranged by size:
5054 1321 404
BssHII G'CGCG_C
Cuts at: 3692 3692
Size: 6779
BssSI C'ACGA_G
Cuts at: 3549 5050 5758 3549
Size: 1501 708 4570
Fragments arranged by size:
4570 1501 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2220 4156 2220
Size: 1936 4843
BstBI TT'CG_AA
Cuts at: 5140 5140
Size: 6779
BstEII G'GTnAC_C
Cuts at: 150 1272 1434 2658 150
Size: 1122 162 1224 4271
Fragments arranged by size:
4271 1224 1122 162
BstXI CCAn_nnnn'nTGG
Cuts at: 114 436 1911 2113 114
Size: 322 1475 202 4780
Fragments arranged by size:
4780 1475 322 202
BstYI r'GATC_y
Cuts at: 1 584 2489 2525 3466 3583 3804 4271 4294
Size: 583 1905 36 941 117 221 467 23
Cuts at: 4294 4629 4875 5181 5193 5279 6331 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1905 1052 941 583 467 449 335 306
246 221 117 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 1514 1514
Size: 6779
BtgI C'CryG_G
Cuts at: 143 158 419 1439 2493 3044 143
Size: 15 261 1020 1054 551 3878
Fragments arranged by size:
3878 1054 1020 551 261 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3558 4729 4910 5073 3558
Size: 1171 181 163 5264
Fragments arranged by size:
5264 1171 181 163
BtsI GCAGTG_nn'
Cuts at: 2699 3412 3515 4022 2699
Size: 713 103 507 5456
Fragments arranged by size:
5456 713 507 103
ClaI AT'CG_AT
Cuts at: 3236 3236
Size: 6779
DraI TTT'AAA
Cuts at: 630 1473 3778 630
Size: 843 2305 3631
Fragments arranged by size:
3631 2305 843
DrdI GACnn_nn'nnGTC
Cuts at: 1674 2160 4485 5829 1674
Size: 486 2325 1344 2624
Fragments arranged by size:
2624 2325 1344 486
EaeI y'GGCC_r
Cuts at: 731 2871 3384 3623 3699 4364 4538 4929 4956
Size: 2140 513 239 76 665 174 391 27
Cuts at: 4956 6301 731
Size: 1345 1209
Fragments arranged by size:
2140 1345 1209 665 513 391 239 174
76 27
EagI C'GGCC_G
Cuts at: 4364 4364
Size: 6779
EarI CTCTTCn'nnn_
Cuts at: 3098 4802 5012 3098
Size: 1704 210 4865
Fragments arranged by size:
4865 1704 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 626 1192 3783 4987 5729 5875 6589 626
Size: 566 2591 1204 742 146 714 816
Fragments arranged by size:
2591 1204 816 742 714 566 146
EcoICRI GAG'CTC
Cuts at: 426 3791 426
Size: 3365 3414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 662 909 1693 1840 1855 2224 2405 2668
Size: 265 247 784 147 15 369 181 263
Cuts at: 2668 2977 4604 5036 5389 6051 397
Size: 309 1627 432 353 662 1125
Fragments arranged by size:
1627 1125 784 662 432 369 353 309
265 263 247 181 147 15
EcoNI CCTnn'n_nnAGG
Cuts at: 392 392
Size: 6779
EcoO109I rG'GnC_Cy
Cuts at: 139 510 535 2197 2347 3918 139
Size: 371 25 1662 150 1571 3000
Fragments arranged by size:
3000 1662 1571 371 150 25
EcoRI G'AATT_C
Cuts at: 3783 3783
Size: 6779
EcoRV GAT'ATC
Cuts at: 1349 1349
Size: 6779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4798 4798
Size: 6779
FspI TGC'GCA
Cuts at: 4560 4560
Size: 6779
HaeII r_GCGC'y
Cuts at: 1024 3196 4213 4461 5691 6462 1024
Size: 2172 1017 248 1230 771 1341
Fragments arranged by size:
2172 1341 1230 1017 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 1082 1114 1437 1469 1545 1577 1831
Size: 32 678 32 323 32 76 32 254
Cuts at: 1831 1863 2378 2410 3528 3560 4621 4651 4653
Size: 32 515 32 1118 32 1061 30 2
Cuts at: 4653 4683 372
Size: 30 2468
Fragments arranged by size:
2468 1118 1061 678 515 323 254 76
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1728 1977 3668 3818 1728
Size: 249 1691 150 4689
Fragments arranged by size:
4689 1691 249 150
HindIII A'AGCT_T
Cuts at: 2742 3334 2742
Size: 592 6187
KasI G'GCGC_C
Cuts at: 1020 4209 4457 1020
Size: 3189 248 3342
Fragments arranged by size:
3342 3189 248
KpnI G_GTAC'C
Cuts at: 2979 3799 2979
Size: 820 5959
MluI A'CGCG_T
Cuts at: 6212 6212
Size: 6779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 221 694 1324 2519 2843 5538 5722 221
Size: 473 630 1195 324 2695 184 1278
Fragments arranged by size:
2695 1278 1195 630 473 324 184
MscI TGG'CCA
Cuts at: 2873 3386 4540 2873
Size: 513 1154 5112
Fragments arranged by size:
5112 1154 513
MslI CAynn'nnrTG
Cuts at: 112 214 434 700 3508 4768 4895 112
Size: 102 220 266 2808 1260 127 1996
Fragments arranged by size:
2808 1996 1260 266 220 127 102
MspA1I CmG'CkG
Cuts at: 145 160 1305 2238 2586 3890 4204 4564 5348
Size: 15 1145 933 348 1304 314 360 784
Cuts at: 5348 5593 145
Size: 245 1331
Fragments arranged by size:
1331 1304 1145 933 784 360 348 314
245 15
NaeI GCC'GGC
Cuts at: 191 3676 4960 191
Size: 3485 1284 2010
Fragments arranged by size:
3485 2010 1284
NarI GG'CG_CC
Cuts at: 1021 4210 4458 1021
Size: 3189 248 3342
Fragments arranged by size:
3342 3189 248
NcoI C'CATG_G
Cuts at: 3044 3044
Size: 6779
NgoMIV G'CCGG_C
Cuts at: 189 3674 4958 189
Size: 3485 1284 2010
Fragments arranged by size:
3485 2010 1284
NsiI A_TGCA'T
Cuts at: 1790 1790
Size: 6779
NspI r_CATG'y
Cuts at: 1881 3832 4332 5935 1881
Size: 1951 500 1603 2725
Fragments arranged by size:
2725 1951 1603 500
PasI CC'CwG_GG
Cuts at: 531 2473 531
Size: 1942 4837
PciI A'CATG_T
Cuts at: 5931 5931
Size: 6779
PflMI CCAn_nnn'nTGG
Cuts at: 874 1086 874
Size: 212 6567
PfoI T'CCnGG_A
Cuts at: 1364 1364
Size: 6779
PmeI GTTT'AAAC
Cuts at: 3778 3778
Size: 6779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1060 1092 1121 1153 2032 2064 3097 3129 5225
Size: 32 29 32 879 32 1033 32 2096
Cuts at: 5225 5257 1060
Size: 32 2582
Fragments arranged by size:
2582 2096 1033 879 32 32 32 32
32 29
PpuMI rG'GwC_Cy
Cuts at: 510 535 510
Size: 25 6754
PshAI GACnn'nnGTC
Cuts at: 896 896
Size: 6779
PsiI TTA'TAA
Cuts at: 1958 1958
Size: 6779
PspOMI G'GGCC_C
Cuts at: 139 139
Size: 6779
PstI C_TGCA'G
Cuts at: 1854 2694 3073 3665 3826 4511 1854
Size: 840 379 592 161 685 4122
Fragments arranged by size:
4122 840 685 592 379 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6779
PvuII CAG'CTG
Cuts at: 1305 2238 4204 4564 1305
Size: 933 1966 360 3520
Fragments arranged by size:
3520 1966 933 360
RsrII CG'GwC_CG
Cuts at: 4974 4974
Size: 6779
SacI G_AGCT'C
Cuts at: 428 3793 428
Size: 3365 3414
SacII CC_GC'GG
Cuts at: 146 161 146
Size: 15 6764
SalI G'TCGA_C
Cuts at: 3816 3816
Size: 6779
SapI GCTCTTCn'nnn_
Cuts at: 4802 5012 4802
Size: 210 6569
SbfI CC_TGCA'GG
Cuts at: 3826 3826
Size: 6779
ScaI AGT'ACT
Cuts at: 6296 6296
Size: 6779
SfcI C'TryA_G
Cuts at: 33 1672 1850 2619 2690 3069 3661 3679 3822
Size: 1639 178 769 71 379 592 18 143
Cuts at: 3822 4507 5475 5666 33
Size: 685 968 191 1146
Fragments arranged by size:
1639 1146 968 769 685 592 379 191
178 143 71 18
SfoI GGC'GCC
Cuts at: 1022 4211 4459 1022
Size: 3189 248 3342
Fragments arranged by size:
3342 3189 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6779
SmaI CCC'GGG
Cuts at: 2780 3801 2780
Size: 1021 5758
SmlI C'TyrA_G
Cuts at: 1207 1412 3160 3491 3934 4849 5563 5825 1207
Size: 205 1748 331 443 915 714 262 2161
Fragments arranged by size:
2161 1748 915 714 443 331 262 205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6779
SphI G_CATG'C
Cuts at: 3832 4332 3832
Size: 500 6279
StuI AGG'CCT
Cuts at: 2507 2722 2507
Size: 215 6564
StyI C'CwwG_G
Cuts at: 393 2112 2186 3044 3381 3913 4267 393
Size: 1719 74 858 337 532 354 2905
Fragments arranged by size:
2905 1719 858 532 354 337 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 589 1588 4362 4495 4954 5163 589
Size: 999 2774 133 459 209 2205
Fragments arranged by size:
2774 2205 999 459 209 133
TatI w'GTAC_w
Cuts at: 281 1602 2006 2651 6294 281
Size: 1321 404 645 3643 766
Fragments arranged by size:
3643 1321 766 645 404
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 536 568 966 998 6296 6328 536
Size: 32 398 32 5298 32 987
Fragments arranged by size:
5298 987 398 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1372 4576 1372
Size: 3204 3575
XbaI T'CTAG_A
Cuts at: 3810 3810
Size: 6779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2110 3051 4274 2110
Size: 941 1223 4615
Fragments arranged by size:
4615 1223 941
XmaI C'CCGG_G
Cuts at: 2778 3799 2778
Size: 1021 5758
XmnI GAAnn'nnTTC
Cuts at: 2902 3314 2902
Size: 412 6367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI BaeI BmtI BseRI
BsiWI BsmBI BspEI BstZ17I CspCI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI