Restriction map for
pF1KB1123
(Circular) (Six-base) MAPSORT of: pF1KB1123.seq Check: 1197 from: 1 to: 10760
pF1KB1123 10760 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1527 1990 2610 1527
Size: 463 620 9677
Fragments arranged by size:
9677 620 463
AatII G_ACGT'C
Cuts at: 5973 5973
Size: 10760
AccI GT'mk_AC
Cuts at: 286 6052 7798 286
Size: 5766 1746 3248
Fragments arranged by size:
5766 3248 1746
Acc65I G'GTAC_C
Cuts at: 6005 7776 6005
Size: 1771 8989
AclI AA'CG_TT
Cuts at: 10600 10600
Size: 10760
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2455 2743 3503 4003 4181 6112 6322 8585 9017
Size: 288 760 500 178 1931 210 2263 432
Cuts at: 9017 9764 10032 2455
Size: 747 268 3183
Fragments arranged by size:
3183 2263 1931 760 747 500 432 288
268 210 178
AfeI AGC'GCT
Cuts at: 2152 3512 3730 2152
Size: 1360 218 9182
Fragments arranged by size:
9182 1360 218
AflIII A'CryG_T
Cuts at: 5216 6098 8112 9216 10193 5216
Size: 882 2014 1104 977 5783
Fragments arranged by size:
5783 2014 1104 977 882
AgeI A'CCGG_T
Cuts at: 9148 9148
Size: 10760
AhdI GACnn_n'nnGTC
Cuts at: 3636 3636
Size: 10760
AleI CACnn'nnGTG
Cuts at: 1174 1663 4791 1174
Size: 489 3128 7143
Fragments arranged by size:
7143 3128 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 197 1106 1585 4267 4809 5100 6928 197
Size: 909 479 2682 542 291 1828 4029
Fragments arranged by size:
4029 2682 1828 909 542 479 291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3127 3159 4301 4333 3127
Size: 32 1142 32 9554
Fragments arranged by size:
9554 1142 32 32
AlwNI CAG_nnn'CTG
Cuts at: 987 2107 2470 3075 3153 5334 6300 8065 9632
Size: 1120 363 605 78 2181 966 1765 1567
Cuts at: 9632 987
Size: 2115
Fragments arranged by size:
2181 2115 1765 1567 1120 966 605 363
78
ApaI G_GGCC'C
Cuts at: 4023 4219 6292 4023
Size: 196 2073 8491
Fragments arranged by size:
8491 2073 196
ApaLI G'TGCA_C
Cuts at: 9530 9530
Size: 10760
ApoI r'AATT_y
Cuts at: 544 1213 1885 2715 3136 3226 3304 3674 4192
Size: 669 672 830 421 90 78 370 518
Cuts at: 4192 7475 7764 10113 10369 544
Size: 3283 289 2349 256 935
Fragments arranged by size:
3283 2349 935 830 672 669 518 421
370 289 256 90 78
AseI AT'TA_AT
Cuts at: 20 2443 4364 20
Size: 2423 1921 6416
Fragments arranged by size:
6416 2423 1921
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10760
AvaI C'yCGr_G
Cuts at: 1577 2834 4558 4718 4933 6807 7208 7780 1577
Size: 1257 1724 160 215 1874 401 572 4557
Fragments arranged by size:
4557 1874 1724 1257 572 401 215 160
BamHI G'GATC_C
Cuts at: 887 2790 3789 7785 887
Size: 1903 999 3996 3862
Fragments arranged by size:
3996 3862 1903 999
BanI G'GyrC_C
Cuts at: 2381 3418 5751 6005 7742 7776 8190 8438 8473
Size: 1037 2333 254 1737 34 414 248 35
Cuts at: 8473 2381
Size: 4668
Fragments arranged by size:
4668 2333 1737 1037 414 254 248 35
34
BanII G_rGCy'C
Cuts at: 239 564 1628 2678 3607 4023 4118 4219 6266
Size: 325 1064 1050 929 416 95 101 2047
Cuts at: 6266 6292 7774 8804 239
Size: 26 1482 1030 2195
Fragments arranged by size:
2195 2047 1482 1064 1050 1030 929 416
325 101 95 26
BbeI G_GCGC'C
Cuts at: 3422 8194 8442 3422
Size: 4772 248 5740
Fragments arranged by size:
5740 4772 248
BbsI GAAGACnn'nnnn_
Cuts at: 1545 2731 3342 3632 5057 1545
Size: 1186 611 290 1425 7248
Fragments arranged by size:
7248 1425 1186 611 290
BbvCI CC'TCA_GC
Cuts at: 5927 6455 6766 5927
Size: 528 311 9921
Fragments arranged by size:
9921 528 311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 771 805 2067 2101 90
Size: 34 647 34 1262 34 8749
Fragments arranged by size:
8749 1262 647 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3757 5805 8658 9419 10760 3757
Size: 2048 2853 761 1341 3757
Fragments arranged by size:
3757 2853 2048 1341 761
BclI T'GATC_A
Cuts at: 1218 2489 8280 1218
Size: 1271 5791 3698
Fragments arranged by size:
5791 3698 1271
BfrBI ATG'CAT
Cuts at: 3357 3838 3357
Size: 481 10279
BglI GCCn_nnn'nGGC
Cuts at: 1772 4489 4642 4651 5087 5572 5608 6818 7286
Size: 2717 153 9 436 485 36 1210 468
Cuts at: 7286 1772
Size: 5246
Fragments arranged by size:
5246 2717 1210 485 468 436 153 36
9
BglII A'GATC_T
Cuts at: 1011 4958 6678 8275 10312 1011
Size: 3947 1720 1597 2037 1459
Fragments arranged by size:
3947 2037 1720 1597 1459
BlpI GC'TnA_GC
Cuts at: 2074 3967 7872 2074
Size: 1893 3905 4962
Fragments arranged by size:
4962 3905 1893
Bme1580I G_kGCm'C
Cuts at: 2370 2384 4023 4219 5232 6292 7747 8385 8478
Size: 14 1639 196 1013 1060 1455 638 93
Cuts at: 8478 9534 2370
Size: 1056 3596
Fragments arranged by size:
3596 1639 1455 1060 1056 1013 638 196
93 14
BmgBI CAC'GTC
Cuts at: 3885 3885
Size: 10760
BmrI ACTGGGnnnn_n'
Cuts at: 460 1148 1332 7972 8053 8388 10532 460
Size: 688 184 6640 81 335 2144 688
Fragments arranged by size:
6640 2144 688 688 335 184 81
BmtI G_CTAG'C
Cuts at: 383 383
Size: 10760
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7645 9127 7645
Size: 1482 9278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1642 2575 3994 4333 4456 4924 5791 6280
Size: 1419 933 1419 339 123 468 867 489
Cuts at: 6280 7413 223
Size: 1133 3570
Fragments arranged by size:
3570 1419 1419 1133 933 867 489 468
339 123
Bpu10I CC'TnA_GC
Cuts at: 1369 1678 3817 5927 6455 6766 1369
Size: 309 2139 2110 528 311 5363
Fragments arranged by size:
5363 2139 2110 528 311 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2722 5053 7936 8815 9307 9605 2722
Size: 2331 2883 879 492 298 3877
Fragments arranged by size:
3877 2883 2331 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 841 2394 2981 3936 4621 10464 841
Size: 1553 587 955 685 5843 1137
Fragments arranged by size:
5843 1553 1137 955 685 587
BsaAI yAC'GTr
Cuts at: 540 8113 540
Size: 7573 3187
BsaBI GATnn'nnATC
Cuts at: 6 2308 2977 8274 10311 6
Size: 2302 669 5297 2037 455
Fragments arranged by size:
5297 2302 2037 669 455
BsaHI Gr'CG_yC
Cuts at: 1544 2137 3419 5970 8191 8439 9141 10637 1544
Size: 593 1282 2551 2221 248 702 1496 1667
Fragments arranged by size:
2551 2221 1667 1496 1282 702 593 248
BsaWI w'CCGG_w
Cuts at: 118 6924 7949 8470 9148 9422 9569 9980 118
Size: 6806 1025 521 678 274 147 411 898
Fragments arranged by size:
6806 1025 898 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1171 1201 2374 2404 3547 3577 4762 4792 1171
Size: 30 1173 30 1143 30 1185 30 7139
Fragments arranged by size:
7139 1185 1173 1143 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 439 1222 3799 4921 6163 295
Size: 144 783 2577 1122 1242 4892
Fragments arranged by size:
4892 2577 1242 1122 783 144
BseYI C'CCAG_C
Cuts at: 573 770 1079 1107 2376 2949 3153 3323 3657
Size: 197 309 28 1269 573 204 170 334
Cuts at: 3657 4016 4254 4539 5291 6821 6991 7030 8185
Size: 359 238 285 752 1530 170 39 1155
Cuts at: 8185 8943 9520 10016 573
Size: 758 577 496 1317
Fragments arranged by size:
1530 1317 1269 1155 758 752 577 573
496 359 334 309 285 238 204 197
170 170 39 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2229 3088 3865 4450 5320 6381 6829 7153 2229
Size: 859 777 585 870 1061 448 324 5836
Fragments arranged by size:
5836 1061 870 859 777 585 448 324
BsiEI CG_ry'CG
Cuts at: 104 4397 5457 5612 8302 8348 9556 104
Size: 4293 1060 155 2690 46 1208 1308
Fragments arranged by size:
4293 2690 1308 1208 1060 155 46
BsiHKAI G_wGCw'C
Cuts at: 239 1670 1952 2336 2678 3259 3743 4118 5410
Size: 1431 282 384 342 581 484 375 1292
Cuts at: 5410 5453 5674 6110 7337 7774 8552 8742 9534
Size: 43 221 436 1227 437 778 190 792
Cuts at: 9534 239
Size: 1465
Fragments arranged by size:
1465 1431 1292 1227 792 778 581 484
437 436 384 375 342 282 221 190
43
BsmI GAATG_Cn'
Cuts at: 411 1309 6563 411
Size: 898 5254 4608
Fragments arranged by size:
5254 4608 898
BsmBI CGTCTCn'nnnn_
Cuts at: 3954 5156 5977 7553 3954
Size: 1202 821 1576 7161
Fragments arranged by size:
7161 1576 1202 821
Bsp1286I G_dGCh'C
Cuts at: 239 564 1628 1670 1952 2336 2370 2384 2678
Size: 325 1064 42 282 384 34 14 294
Cuts at: 2678 3259 3607 3743 4023 4118 4219 5232 5410
Size: 581 348 136 280 95 101 1013 178
Cuts at: 5410 5453 5674 6110 6266 6292 7337 7747 7774
Size: 43 221 436 156 26 1045 410 27
Cuts at: 7774 8385 8478 8552 8742 8804 9534 239
Size: 611 93 74 190 62 730 1465
Fragments arranged by size:
1465 1064 1045 1013 730 611 581 436
410 384 348 325 294 282 280 221
190 178 156 136 101 95 93 74
62 43 42 34 27 26 14
BspEI T'CCGG_A
Cuts at: 6924 9980 6924
Size: 3056 7704
BspHI T'CATG_A
Cuts at: 3218 3236 5046 7650 9936 3218
Size: 18 1810 2604 2286 4042
Fragments arranged by size:
4042 2604 2286 1810 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 1527 1990 2610 3326 3446 4823 7810 8024
Size: 1395 463 620 716 120 1377 2987 214
Cuts at: 8024 8326 8707 132
Size: 302 381 2185
Fragments arranged by size:
2987 2185 1395 1377 716 620 463 381
302 214 120
BsrBI CCG'CTC
Cuts at: 50 1489 9052 9106 10590 10755 50
Size: 1439 7563 54 1484 165 55
Fragments arranged by size:
7563 1484 1439 165 55 54
BsrDI GCAATG_nn'
Cuts at: 165 594 1538 1589 4844 5933 8672 165
Size: 429 944 51 3255 1089 2739 2253
Fragments arranged by size:
3255 2739 2253 1089 944 429 51
BsrFI r'CCGG_y
Cuts at: 1184 8758 8939 9148 1184
Size: 7574 181 209 2796
Fragments arranged by size:
7574 2796 209 181
BsrGI T'GTAC_A
Cuts at: 1071 5744 1071
Size: 4673 6087
BssSI C'ACGA_G
Cuts at: 4468 5792 9031 9389 4468
Size: 1324 3239 358 5839
Fragments arranged by size:
5839 3239 1324 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3075 3447 3813 4553 5334 8137 3075
Size: 372 366 740 781 2803 5698
Fragments arranged by size:
5698 2803 781 740 372 366
BstBI TT'CG_AA
Cuts at: 3588 5501 9121 3588
Size: 1913 3620 5227
Fragments arranged by size:
5227 3620 1913
BstEII G'GTnAC_C
Cuts at: 5193 5193
Size: 10760
BstXI CCAn_nnnn'nTGG
Cuts at: 1176 1788 1842 3415 3627 5961 7266 1176
Size: 612 54 1573 212 2334 1305 4670
Fragments arranged by size:
4670 2334 1573 1305 612 212 54
BstYI r'GATC_y
Cuts at: 1 887 1011 1958 2103 2225 2460 2790 2978
Size: 886 124 947 145 122 235 330 188
Cuts at: 2978 3789 4053 4439 4958 5721 6678 6881 7163
Size: 811 264 386 519 763 957 203 282
Cuts at: 7163 7785 8252 8275 8610 8856 9868 9954 9966
Size: 622 467 23 335 246 1012 86 12
Cuts at: 9966 10312 1
Size: 346 449
Fragments arranged by size:
1012 957 947 886 811 763 622 519
467 449 386 346 335 330 282 264
246 235 203 188 145 124 122 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1829 5189 1829
Size: 3360 7400
BtgI C'CryG_G
Cuts at: 254 509 891 2960 3014 3626 4874 5606 254
Size: 255 382 2069 54 612 1248 732 5408
Fragments arranged by size:
5408 2069 1248 732 612 382 255 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1853 3980 4140 5286 5555 5678 6886 8710 8891
Size: 2127 160 1146 269 123 1208 1824 181
Cuts at: 8891 9054 1853
Size: 163 3559
Fragments arranged by size:
3559 2127 1824 1208 1146 269 181 163
160 123
BtsI GCAGTG_nn'
Cuts at: 1643 1658 1670 2057 3490 4600 4759 5495 5594
Size: 15 12 387 1433 1110 159 736 99
Cuts at: 5594 5615 5627 6841 8003 1643
Size: 21 12 1214 1162 4400
Fragments arranged by size:
4400 1433 1214 1162 1110 736 387 159
99 21 15 12 12
ClaI AT'CG_AT
Cuts at: 5240 5240
Size: 10760
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 399 434 1520 1555 399
Size: 35 1086 35 9604
Fragments arranged by size:
9604 1086 35 35
DraI TTT'AAA
Cuts at: 7759 7759
Size: 10760
DraIII CAC_nnn'GTG
Cuts at: 2808 7314 2808
Size: 4506 6254
DrdI GACnn_nn'nnGTC
Cuts at: 469 1549 8466 9324 469
Size: 1080 6917 858 1905
Fragments arranged by size:
6917 1905 1080 858
EaeI y'GGCC_r
Cuts at: 371 498 632 695 854 2022 2237 2609 2651
Size: 127 134 63 159 1168 215 372 42
Cuts at: 2651 3825 4046 4394 5609 5854 6164 6734 7004
Size: 1174 221 348 1215 245 310 570 270
Cuts at: 7004 7352 7461 8345 8519 8910 8937 10282 371
Size: 348 109 884 174 391 27 1345 849
Fragments arranged by size:
1345 1215 1174 1168 884 849 570 391
372 348 348 310 270 245 221 215
174 159 134 127 109 63 42 27
EagI C'GGCC_G
Cuts at: 4394 5609 8345 4394
Size: 1215 2736 6809
Fragments arranged by size:
6809 2736 1215
EarI CTCTTCn'nnn_
Cuts at: 3172 3776 4063 4780 5218 6197 6715 6758 8783
Size: 604 287 717 438 979 518 43 2025
Cuts at: 8783 8993 3172
Size: 210 4939
Fragments arranged by size:
4939 2025 979 717 604 518 438 287
210 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2401 4942 8968 9278 9424 10570 2191
Size: 210 2541 4026 310 146 1146 2381
Fragments arranged by size:
4026 2541 2381 1146 310 210 146
EcoICRI GAG'CTC
Cuts at: 237 2676 4116 7772 237
Size: 2439 1440 3656 3225
Fragments arranged by size:
3656 3225 2439 1440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1642 2455 2575 2743 3503 3994 4003 4181
Size: 1419 813 120 168 760 491 9 178
Cuts at: 4181 4333 4456 4924 5791 6112 6280 6322 7413
Size: 152 123 468 867 321 168 42 1091
Cuts at: 7413 8585 9017 9764 10032 223
Size: 1172 432 747 268 951
Fragments arranged by size:
1419 1172 1091 951 867 813 760 747
491 468 432 321 268 178 168 168
152 123 120 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1828 2170 3079 3904 4027 6202 1828
Size: 342 909 825 123 2175 6386
Fragments arranged by size:
6386 2175 909 825 342 123
EcoO109I rG'GnC_Cy
Cuts at: 705 1145 1785 3865 4020 4215 4216 4327 4770
Size: 440 640 2080 155 195 1 111 443
Cuts at: 4770 4812 6018 6288 6289 6393 6411 6510 6611
Size: 42 1206 270 1 104 18 99 101
Cuts at: 6611 7581 7899 705
Size: 970 318 3566
Fragments arranged by size:
3566 2080 1206 970 640 443 440 318
270 195 155 111 104 101 99 42
18 1 1
EcoRI G'AATT_C
Cuts at: 3226 4192 7764 3226
Size: 966 3572 6222
Fragments arranged by size:
6222 3572 966
EcoRV GAT'ATC
Cuts at: 1608 1608
Size: 10760
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8779 8779
Size: 10760
FspI TGC'GCA
Cuts at: 2299 8541 2299
Size: 6242 4518
HaeII r_GCGC'y
Cuts at: 2154 3422 3514 3732 3920 5441 8194 8442 9464
Size: 1268 92 218 188 1521 2753 248 1022
Cuts at: 9464 10443 2154
Size: 979 2471
Fragments arranged by size:
2753 2471 1521 1268 1022 979 248 218
188 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2088 2120 2744 2776 3547 3579 3622 3654 3701
Size: 32 624 32 771 32 43 32 47
Cuts at: 3701 3733 3979 4011 4762 4794 4842 4874 4931
Size: 32 246 32 751 32 48 32 57
Cuts at: 4931 4934 4963 4966 5710 5742 6152 6184 6733
Size: 3 29 3 744 32 410 32 549
Cuts at: 6733 6765 6770 6802 7438 7470 8602 8632 8634
Size: 32 5 32 636 32 1132 30 2
Cuts at: 8634 8664 2088
Size: 30 4184
Fragments arranged by size:
4184 1132 771 751 744 636 624 549
410 246 57 48 47 43 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 29 5 3
3 2
HincII GTy'rAC
Cuts at: 2916 4582 6723 7256 7799 2916
Size: 1666 2141 533 543 5877
Fragments arranged by size:
5877 2141 1666 543 533
HpaI GTT'AAC
Cuts at: 6723 6723
Size: 10760
KasI G'GCGC_C
Cuts at: 3418 8190 8438 3418
Size: 4772 248 5740
Fragments arranged by size:
5740 4772 248
KpnI G_GTAC'C
Cuts at: 6009 7780 6009
Size: 1771 8989
MluI A'CGCG_T
Cuts at: 10193 10193
Size: 10760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1338 2309 6456 7157 7696 9431 9615 1338
Size: 971 4147 701 539 1735 184 2483
Fragments arranged by size:
4147 2483 1735 971 701 539 184
MscI TGG'CCA
Cuts at: 373 634 856 2024 2239 2611 2653 3827 4048
Size: 261 222 1168 215 372 42 1174 221
Cuts at: 4048 5856 6166 6736 7006 7463 8521 373
Size: 1808 310 570 270 457 1058 2612
Fragments arranged by size:
2612 1808 1174 1168 1058 570 457 372
310 270 261 222 221 215 42
MslI CAynn'nnrTG
Cuts at: 391 478 691 1174 1567 1663 2030 2584 2764
Size: 87 213 483 393 96 367 554 180
Cuts at: 2764 2818 3217 3625 3685 4708 4791 6139 7090
Size: 54 399 408 60 1023 83 1348 951
Cuts at: 7090 7315 7354 8749 8876 391
Size: 225 39 1395 127 2275
Fragments arranged by size:
2275 1395 1348 1023 951 554 483 408
399 393 367 225 213 180 127 96
87 83 60 54 39
MspA1I CmG'CkG
Cuts at: 142 532 610 987 1402 1800 2953 3153 3327
Size: 390 78 377 415 398 1153 200 174
Cuts at: 3327 3411 3657 4393 4663 5382 5462 5570 5700
Size: 84 246 736 270 719 80 108 130
Cuts at: 5700 6450 6991 7871 8185 8545 9558 9803 142
Size: 750 541 880 314 360 1013 245 1099
Fragments arranged by size:
1153 1099 1013 880 750 736 719 541
415 398 390 377 360 314 270 246
245 200 174 130 108 84 80 78
NaeI GCC'GGC
Cuts at: 8941 8941
Size: 10760
NarI GG'CG_CC
Cuts at: 3419 8191 8439 3419
Size: 4772 248 5740
Fragments arranged by size:
5740 4772 248
NcoI C'CATG_G
Cuts at: 254 509 891 2960 3014 3626 4874 254
Size: 255 382 2069 54 612 1248 6140
Fragments arranged by size:
6140 2069 1248 612 382 255 54
NdeI CA'TA_TG
Cuts at: 4284 4866 6537 4284
Size: 582 1671 8507
Fragments arranged by size:
8507 1671 582
NgoMIV G'CCGG_C
Cuts at: 8939 8939
Size: 10760
NheI G'CTAG_C
Cuts at: 379 379
Size: 10760
NsiI A_TGCA'T
Cuts at: 3359 3840 3359
Size: 481 10279
NspI r_CATG'y
Cuts at: 607 1572 1779 2376 2589 3342 4551 4893 5220
Size: 965 207 597 213 753 1209 342 327
Cuts at: 5220 5259 5299 5482 6102 6249 6274 6976 7307
Size: 39 40 183 620 147 25 702 331
Cuts at: 7307 7813 8313 9220 607
Size: 506 500 907 2147
Fragments arranged by size:
2147 1209 965 907 753 702 620 597
506 500 342 331 327 213 207 183
147 40 39 25
PasI CC'CwG_GG
Cuts at: 1416 2637 3051 1416
Size: 1221 414 9125
Fragments arranged by size:
9125 1221 414
PciI A'CATG_T
Cuts at: 5216 6098 9216 5216
Size: 882 3118 6760
Fragments arranged by size:
6760 3118 882
PflMI CCAn_nnn'nTGG
Cuts at: 1416 1800 3153 6173 1416
Size: 384 1353 3020 6003
Fragments arranged by size:
6003 3020 1353 384
PfoI T'CCnGG_A
Cuts at: 2228 2591 3131 3565 5807 6857 2228
Size: 363 540 434 2242 1050 6131
Fragments arranged by size:
6131 2242 1050 540 434 363
PmeI GTTT'AAAC
Cuts at: 7759 7759
Size: 10760
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1020 1052 9899 9931 1020
Size: 32 8847 32 1849
Fragments arranged by size:
8847 1849 32 32
PpuMI rG'GwC_Cy
Cuts at: 705 1145 3865 4770 4812 6393 6411 6510 705
Size: 440 2720 905 42 1581 18 99 4955
Fragments arranged by size:
4955 2720 1581 905 440 99 42 18
PshAI GACnn'nnGTC
Cuts at: 5999 5999
Size: 10760
PspOMI G'GGCC_C
Cuts at: 4019 4215 6288 4019
Size: 196 2073 8491
Fragments arranged by size:
8491 2073 196
PspXI vC'TCGA_Gb
Cuts at: 1577 4558 1577
Size: 2981 7779
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7050 7082 7050
Size: 32 10728
PstI C_TGCA'G
Cuts at: 176 1665 3751 4662 4979 5429 5705 7807 8492
Size: 1489 2086 911 317 450 276 2102 685
Cuts at: 8492 176
Size: 2444
Fragments arranged by size:
2444 2102 2086 1489 911 685 450 317
276
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10760
PvuII CAG'CTG
Cuts at: 610 987 1800 2953 3153 3327 3411 3657 4663
Size: 377 813 1153 200 174 84 246 1006
Cuts at: 4663 5382 5462 6450 6991 8185 8545 610
Size: 719 80 988 541 1194 360 2825
Fragments arranged by size:
2825 1194 1153 1006 988 813 719 541
377 360 246 200 174 84 80
RsrII CG'GwC_CG
Cuts at: 8955 8955
Size: 10760
SacI G_AGCT'C
Cuts at: 239 2678 4118 7774 239
Size: 2439 1440 3656 3225
Fragments arranged by size:
3656 3225 2439 1440
SalI G'TCGA_C
Cuts at: 7797 7797
Size: 10760
SanDI GG'GwC_CC
Cuts at: 705 705
Size: 10760
SapI GCTCTTCn'nnn_
Cuts at: 3172 6715 8783 8993 3172
Size: 3543 2068 210 4939
Fragments arranged by size:
4939 3543 2068 210
SbfI CC_TGCA'GG
Cuts at: 7807 7807
Size: 10760
ScaI AGT'ACT
Cuts at: 1003 5431 10277 1003
Size: 4428 4846 1486
Fragments arranged by size:
4846 4428 1486
SexAI A'CCwGG_T
Cuts at: 2213 2384 4450 2213
Size: 171 2066 8523
Fragments arranged by size:
8523 2066 171
SfcI C'TryA_G
Cuts at: 33 172 734 1661 2679 2892 3747 4375 4658
Size: 139 562 927 1018 213 855 628 283
Cuts at: 4658 4975 5389 5425 5433 5701 5914 6864 7293
Size: 317 414 36 8 268 213 950 429
Cuts at: 7293 7803 8488 9481 9672 33
Size: 510 685 993 191 1121
Fragments arranged by size:
1121 1018 993 950 927 855 685 628
562 510 429 414 317 283 268 213
213 191 139 36 8
SfoI GGC'GCC
Cuts at: 3420 8192 8440 3420
Size: 4772 248 5740
Fragments arranged by size:
5740 4772 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10760
SmaI CCC'GGG
Cuts at: 2836 7782 2836
Size: 4946 5814
SmlI C'TyrA_G
Cuts at: 1577 2701 4558 5068 7915 8830 9322 9584 1577
Size: 1124 1857 510 2847 915 492 262 2753
Fragments arranged by size:
2847 2753 1857 1124 915 510 492 262
SpeI A'CTAG_T
Cuts at: 67 2899 67
Size: 2832 7928
SphI G_CATG'C
Cuts at: 1572 1779 2376 4551 5299 5482 6274 6976 7813
Size: 207 597 2175 748 183 792 702 837
Cuts at: 7813 8313 1572
Size: 500 4019
Fragments arranged by size:
4019 2175 837 792 748 702 597 500
207 183
StuI AGG'CCT
Cuts at: 1130 2191 3910 4033 4564 5473 5872 6197 6325
Size: 1061 1719 123 531 909 399 325 128
Cuts at: 6325 7112 7280 1130
Size: 787 168 4610
Fragments arranged by size:
4610 1719 1061 909 787 531 399 325
168 128 123
StyI C'CwwG_G
Cuts at: 230 254 488 509 851 891 1094 1125 1781
Size: 24 234 21 342 40 203 31 656
Cuts at: 1781 2960 3014 3626 3828 4565 4781 4874 5960
Size: 1179 54 612 202 737 216 93 1086
Cuts at: 5960 7894 8248 230
Size: 1934 354 2742
Fragments arranged by size:
2742 1934 1179 1086 737 656 612 354
342 234 216 203 202 93 54 40
31 24 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 1224 1784 4246 4741 6415 7067 7142 8343
Size: 659 560 2462 495 1674 652 75 1201
Cuts at: 8343 8476 8935 9144 565
Size: 133 459 209 2181
Fragments arranged by size:
2462 2181 1674 1201 659 652 560 495
459 209 133 75
TatI w'GTAC_w
Cuts at: 1001 1071 4919 5429 5744 6218 6368 10275 1001
Size: 70 3848 510 315 474 150 3907 1486
Fragments arranged by size:
3907 3848 1486 510 474 315 150 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1171 1203 1991 2023 2374 2406 2633 2665 3149
Size: 32 788 32 351 32 227 32 484
Cuts at: 3149 3181 4879 4911 10277 10309 1171
Size: 32 1698 32 5366 32 1622
Fragments arranged by size:
5366 1698 1622 788 484 351 227 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3537 7345 8557 3537
Size: 3808 1212 5740
Fragments arranged by size:
5740 3808 1212
XbaI T'CTAG_A
Cuts at: 4344 7791 4344
Size: 3447 7313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1635 2017 2247 2619 4815 5961 7140 7732 8255
Size: 382 230 372 2196 1146 1179 592 523
Cuts at: 8255 1635
Size: 4140
Fragments arranged by size:
4140 2196 1179 1146 592 523 382 372
230
XhoI C'TCGA_G
Cuts at: 1577 4558 1577
Size: 2981 7779
XmaI C'CCGG_G
Cuts at: 2834 7780 2834
Size: 4946 5814
XmnI GAAnn'nnTTC
Cuts at: 7541 7541
Size: 10760
ZraI GAC'GTC
Cuts at: 5971 5971
Size: 10760
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AscI AvrII BaeI BsiWI BssHII BstZ17I FseI
FspAI HindIII MfeI NotI NruI PacI PmlI PsiI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI