Restriction map for
pF1KB0998
(Circular) (Six-base) MAPSORT of: pF1KB0998.seq Check: 9005 from: 1 to: 3803
pF1KB0998 3803 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 457 457
Size: 3803
AccI GT'mk_AC
Cuts at: 841 841
Size: 3803
Acc65I G'GTAC_C
Cuts at: 819 819
Size: 3803
AclI AA'CG_TT
Cuts at: 3643 3643
Size: 3803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2060 2807 3075 1628
Size: 432 747 268 2356
Fragments arranged by size:
2356 747 432 268
AflIII A'CryG_T
Cuts at: 1155 2259 3236 1155
Size: 1104 977 1722
Fragments arranged by size:
1722 1104 977
AgeI A'CCGG_T
Cuts at: 2191 2191
Size: 3803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 189 221 189
Size: 32 3771
AlwNI CAG_nnn'CTG
Cuts at: 1108 2675 1108
Size: 1567 2236
ApaLI G'TGCA_C
Cuts at: 2573 2573
Size: 3803
ApoI r'AATT_y
Cuts at: 577 807 3156 3412 577
Size: 230 2349 256 968
Fragments arranged by size:
2349 968 256 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 3803
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
AvaI C'yCGr_G
Cuts at: 440 823 440
Size: 383 3420
AvrII C'CTAG_G
Cuts at: 144 144
Size: 3803
BamHI G'GATC_C
Cuts at: 533 828 533
Size: 295 3508
BanI G'GyrC_C
Cuts at: 217 561 819 1233 1481 1516 217
Size: 344 258 414 248 35 2504
Fragments arranged by size:
2504 414 344 258 248 35
BanII G_rGCy'C
Cuts at: 730 817 1847 730
Size: 87 1030 2686
Fragments arranged by size:
2686 1030 87
BbeI G_GCGC'C
Cuts at: 1237 1485 1237
Size: 248 3555
BbsI GAAGACnn'nnnn_
Cuts at: 417 417
Size: 3803
BciVI GTATCCnnnnn_n'
Cuts at: 1701 2462 3803 1701
Size: 761 1341 1701
Fragments arranged by size:
1701 1341 761
BclI T'GATC_A
Cuts at: 1323 1323
Size: 3803
BglII A'GATC_T
Cuts at: 1318 3355 1318
Size: 2037 1766
BlpI GC'TnA_GC
Cuts at: 915 915
Size: 3803
Bme1580I G_kGCm'C
Cuts at: 169 1428 1521 2577 169
Size: 1259 93 1056 1395
Fragments arranged by size:
1395 1259 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 288 1015 1096 1431 3575 288
Size: 727 81 335 2144 516
Fragments arranged by size:
2144 727 516 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 3803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 979 1858 2350 2648 979
Size: 879 492 298 2134
Fragments arranged by size:
2134 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3507 3507
Size: 3803
BsaAI yAC'GTr
Cuts at: 1156 1156
Size: 3803
BsaBI GATnn'nnATC
Cuts at: 6 1317 3354 6
Size: 1311 2037 455
Fragments arranged by size:
2037 1311 455
BsaHI Gr'CG_yC
Cuts at: 1234 1482 2184 3680 1234
Size: 248 702 1496 1357
Fragments arranged by size:
1496 1357 702 248
BsaWI w'CCGG_w
Cuts at: 992 1513 2191 2465 2612 3023 992
Size: 521 678 274 147 411 1772
Fragments arranged by size:
1772 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 369 369
Size: 3803
BseYI C'CCAG_C
Cuts at: 190 1228 1986 2563 3059 190
Size: 1038 758 577 496 934
Fragments arranged by size:
1038 934 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1345 1391 2599 104
Size: 1241 46 1208 1308
Fragments arranged by size:
1308 1241 1208 46
BsiHKAI G_wGCw'C
Cuts at: 730 817 1595 1785 2577 730
Size: 87 778 190 792 1956
Fragments arranged by size:
1956 792 778 190 87
Bsp1286I G_dGCh'C
Cuts at: 169 730 817 1428 1521 1595 1785 1847 2577
Size: 561 87 611 93 74 190 62 730
Cuts at: 2577 169
Size: 1395
Fragments arranged by size:
1395 730 611 561 190 93 87 74
62
BspEI T'CCGG_A
Cuts at: 3023 3023
Size: 3803
BspHI T'CATG_A
Cuts at: 2979 2979
Size: 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 457 853 1067 1369 1750 457
Size: 396 214 302 381 2510
Fragments arranged by size:
2510 396 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2095 2149 3633 3798 50
Size: 2045 54 1484 165 55
Fragments arranged by size:
2045 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1715 1715
Size: 3803
BsrFI r'CCGG_y
Cuts at: 1801 1982 2191 1801
Size: 181 209 3413
Fragments arranged by size:
3413 209 181
BssSI C'ACGA_G
Cuts at: 2074 2432 2074
Size: 358 3445
BstAPI GCAn_nnn'nTGC
Cuts at: 416 1180 416
Size: 764 3039
BstBI TT'CG_AA
Cuts at: 2164 2164
Size: 3803
BstYI r'GATC_y
Cuts at: 1 533 828 1295 1318 1653 1899 2911 2997
Size: 532 295 467 23 335 246 1012 86
Cuts at: 2997 3009 3355 1
Size: 12 346 449
Fragments arranged by size:
1012 532 467 449 346 335 295 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3803
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1753 1934 2097 1753
Size: 181 163 3459
Fragments arranged by size:
3459 181 163
BtsI GCAGTG_nn'
Cuts at: 1046 1046
Size: 3803
DraI TTT'AAA
Cuts at: 802 802
Size: 3803
DraIII CAC_nnn'GTG
Cuts at: 296 296
Size: 3803
DrdI GACnn_nn'nnGTC
Cuts at: 1509 2367 1509
Size: 858 2945
EaeI y'GGCC_r
Cuts at: 1388 1562 1953 1980 3325 1388
Size: 174 391 27 1345 1866
Fragments arranged by size:
1866 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1388 1388
Size: 3803
EarI CTCTTCn'nnn_
Cuts at: 1826 2036 1826
Size: 210 3593
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2011 2321 2467 3613 2011
Size: 310 146 1146 2201
Fragments arranged by size:
2201 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 815 728
Size: 87 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2060 2807 3075 1628
Size: 432 747 268 2356
Fragments arranged by size:
2356 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 252 942 252
Size: 690 3113
EcoRI G'AATT_C
Cuts at: 807 807
Size: 3803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1822 1822
Size: 3803
FspI TGC'GCA
Cuts at: 1584 1584
Size: 3803
HaeII r_GCGC'y
Cuts at: 1237 1485 2507 3486 1237
Size: 248 1022 979 1554
Fragments arranged by size:
1554 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 407 439 535 567 1645 1675 1677 1707 407
Size: 32 96 32 1078 30 2 30 2503
Fragments arranged by size:
2503 1078 96 32 32 30 30 2
HincII GTy'rAC
Cuts at: 745 842 745
Size: 97 3706
KasI G'GCGC_C
Cuts at: 1233 1481 1233
Size: 248 3555
KpnI G_GTAC'C
Cuts at: 823 823
Size: 3803
MluI A'CGCG_T
Cuts at: 3236 3236
Size: 3803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 707 2474 2658 150
Size: 557 1767 184 1295
Fragments arranged by size:
1767 1295 557 184
MscI TGG'CCA
Cuts at: 1564 1564
Size: 3803
MslI CAynn'nnrTG
Cuts at: 358 1792 1919 358
Size: 1434 127 2242
Fragments arranged by size:
2242 1434 127
MspA1I CmG'CkG
Cuts at: 194 331 914 1228 1588 2601 2846 194
Size: 137 583 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 583 360 314 245 137
NaeI GCC'GGC
Cuts at: 1984 1984
Size: 3803
NarI GG'CG_CC
Cuts at: 1234 1482 1234
Size: 248 3555
NcoI C'CATG_G
Cuts at: 107 107
Size: 3803
NgoMIV G'CCGG_C
Cuts at: 1982 1982
Size: 3803
NspI r_CATG'y
Cuts at: 856 1356 2263 856
Size: 500 907 2396
Fragments arranged by size:
2396 907 500
PciI A'CATG_T
Cuts at: 2259 2259
Size: 3803
PflMI CCAn_nnn'nTGG
Cuts at: 132 739 132
Size: 607 3196
PmeI GTTT'AAAC
Cuts at: 802 802
Size: 3803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2942 2974 2942
Size: 32 3771
PstI C_TGCA'G
Cuts at: 850 1535 850
Size: 685 3118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3803
PvuII CAG'CTG
Cuts at: 194 1228 1588 194
Size: 1034 360 2409
Fragments arranged by size:
2409 1034 360
RsrII CG'GwC_CG
Cuts at: 1998 1998
Size: 3803
SacI G_AGCT'C
Cuts at: 730 817 730
Size: 87 3716
SalI G'TCGA_C
Cuts at: 840 840
Size: 3803
SapI GCTCTTCn'nnn_
Cuts at: 1826 2036 1826
Size: 210 3593
SbfI CC_TGCA'GG
Cuts at: 850 850
Size: 3803
ScaI AGT'ACT
Cuts at: 3320 3320
Size: 3803
SfcI C'TryA_G
Cuts at: 33 306 777 846 1531 2524 2715 33
Size: 273 471 69 685 993 191 1121
Fragments arranged by size:
1121 993 685 471 273 191 69
SfoI GGC'GCC
Cuts at: 1235 1483 1235
Size: 248 3555
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3803
SmaI CCC'GGG
Cuts at: 825 825
Size: 3803
SmlI C'TyrA_G
Cuts at: 958 1873 2365 2627 958
Size: 915 492 262 2134
Fragments arranged by size:
2134 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3803
SphI G_CATG'C
Cuts at: 856 1356 856
Size: 500 3303
StuI AGG'CCT
Cuts at: 149 494 738 149
Size: 345 244 3214
Fragments arranged by size:
3214 345 244
StyI C'CwwG_G
Cuts at: 107 144 248 486 733 937 1291 107
Size: 37 104 238 247 204 354 2619
Fragments arranged by size:
2619 354 247 238 204 104 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 1386 1519 1978 2187 307
Size: 1079 133 459 209 1923
Fragments arranged by size:
1923 1079 459 209 133
TatI w'GTAC_w
Cuts at: 3318 3318
Size: 3803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 252 284 3320 3352 252
Size: 32 3036 32 703
Fragments arranged by size:
3036 703 32 32
Tth111I GACn'n_nGTC
Cuts at: 1600 1600
Size: 3803
XbaI T'CTAG_A
Cuts at: 834 834
Size: 3803
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1298 453
Size: 845 2958
XmaI C'CCGG_G
Cuts at: 823 823
Size: 3803
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI