Restriction map for
pF1KB0994
(Circular) (Six-base) MAPSORT of: pF1KB0994.seq Check: 6263 from: 1 to: 4496
pF1KB0994 4496 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 108 834 108
Size: 726 3770
AccI GT'mk_AC
Cuts at: 953 1534 953
Size: 581 3915
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 807 2321 2753 3500 3768 807
Size: 1514 432 747 268 1535
Fragments arranged by size:
1535 1514 747 432 268
AfeI AGC'GCT
Cuts at: 1456 1456
Size: 4496
AflIII A'CryG_T
Cuts at: 146 312 1848 2952 3929 146
Size: 166 1536 1104 977 713
Fragments arranged by size:
1536 1104 977 713 166
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 355 897 1801 3368 355
Size: 542 904 1567 1483
Fragments arranged by size:
1567 1483 904 542
ApaI G_GGCC'C
Cuts at: 622 1217 1268 622
Size: 595 51 3850
Fragments arranged by size:
3850 595 51
ApaLI G'TGCA_C
Cuts at: 628 3266 628
Size: 2638 1858
ApoI r'AATT_y
Cuts at: 1500 3849 4105 1500
Size: 2349 256 1891
Fragments arranged by size:
2349 1891 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
BamHI G'GATC_C
Cuts at: 1521 1521
Size: 4496
BanI G'GyrC_C
Cuts at: 374 1512 1926 2174 2209 374
Size: 1138 414 248 35 2661
Fragments arranged by size:
2661 1138 414 248 35
BanII G_rGCy'C
Cuts at: 476 622 722 1148 1217 1268 1448 1510 2540
Size: 146 100 426 69 51 180 62 1030
Cuts at: 2540 476
Size: 2432
Fragments arranged by size:
2432 1030 426 180 146 100 69 62
51
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbvCI CC'TCA_GC
Cuts at: 1235 1318 1235
Size: 83 4413
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 572 2016 572
Size: 1444 3052
BglI GCCn_nnn'nGGC
Cuts at: 1231 1231
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 379 595 622 632 1217 1268 2121 2214 3270
Size: 216 27 10 585 51 853 93 1056
Cuts at: 3270 379
Size: 1605
Fragments arranged by size:
1605 1056 853 585 216 93 51 27
10
BmgBI CAC'GTC
Cuts at: 313 588 313
Size: 275 4221
BmrI ACTGGGnnnn_n'
Cuts at: 605 625 1708 1789 2124 4268 605
Size: 20 1083 81 335 2144 833
Fragments arranged by size:
2144 1083 833 335 81 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 508 678 892 1108 1411 1426 1432 1489
Size: 394 170 214 216 303 15 6 57
Cuts at: 1489 114
Size: 3121
Fragments arranged by size:
3121 394 303 216 214 170 57 15
6
Bpu10I CC'TnA_GC
Cuts at: 1235 1318 1235
Size: 83 4413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1672 2551 3043 3341 1267
Size: 405 879 492 298 2422
Fragments arranged by size:
2422 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 147 1296 1849 147
Size: 1149 553 2794
Fragments arranged by size:
2794 1149 553
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 983 1685 2206 2884 3158 3305 3716 983
Size: 702 521 678 274 147 411 1763
Fragments arranged by size:
1763 702 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 868 898 1012 1042 1180 1210 868
Size: 30 114 30 138 30 4154
Fragments arranged by size:
4154 138 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 889 1252 1261 1423 1426 1486 889
Size: 363 9 162 3 60 3899
Fragments arranged by size:
3899 363 162 60 9 3
BseYI C'CCAG_C
Cuts at: 1052 1245 1261 1381 1921 2679 3256 3752 1052
Size: 193 16 120 540 758 577 496 1796
Fragments arranged by size:
1796 758 577 540 496 193 120 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 575 803 1003 1111 1459 402
Size: 173 228 200 108 348 3439
Fragments arranged by size:
3439 348 228 200 173 108
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 632 1448 1510 2288 2478 3270 632
Size: 816 62 778 190 792 1858
Fragments arranged by size:
1858 816 792 778 190 62
BsmI GAATG_Cn'
Cuts at: 330 330
Size: 4496
BsmBI CGTCTCn'nnnn_
Cuts at: 201 201
Size: 4496
Bsp1286I G_dGCh'C
Cuts at: 379 476 595 622 632 722 1148 1217 1268
Size: 97 119 27 10 90 426 69 51
Cuts at: 1268 1448 1510 2121 2214 2288 2478 2540 3270
Size: 180 62 611 93 74 190 62 730
Cuts at: 3270 379
Size: 1605
Fragments arranged by size:
1605 730 611 426 190 180 119 97
93 90 74 69 62 62 51 27
10
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 108 547 834 1546 1760 2062 2443 108
Size: 439 287 712 214 302 381 2161
Fragments arranged by size:
2161 712 439 381 302 287 214
BsrBI CCG'CTC
Cuts at: 50 128 840 1134 2788 2842 4326 4491 50
Size: 78 712 294 1654 54 1484 165 55
Fragments arranged by size:
1654 1484 712 294 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 395 1148 2408 395
Size: 753 1260 2483
Fragments arranged by size:
2483 1260 753
BsrFI r'CCGG_y
Cuts at: 113 159 2494 2675 2884 113
Size: 46 2335 181 209 1725
Fragments arranged by size:
2335 1725 209 181 46
BssSI C'ACGA_G
Cuts at: 760 1129 2767 3125 760
Size: 369 1638 358 2131
Fragments arranged by size:
2131 1638 369 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 2857 2857
Size: 4496
BstXI CCAn_nnnn'nTGG
Cuts at: 151 507 151
Size: 356 4140
BstYI r'GATC_y
Cuts at: 1 437 806 1521 1988 2011 2346 2592 3604
Size: 436 369 715 467 23 335 246 1012
Cuts at: 3604 3690 3702 4048 1
Size: 86 12 346 449
Fragments arranged by size:
1012 715 467 449 436 369 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 332 815 1433 332
Size: 483 618 3395
Fragments arranged by size:
3395 618 483
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 351 2446 2627 2790 351
Size: 2095 181 163 2057
Fragments arranged by size:
2095 2057 181 163
BtsI GCAGTG_nn'
Cuts at: 791 1739 791
Size: 948 3548
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 421 2202 3060 421
Size: 1781 858 1857
Fragments arranged by size:
1857 1781 858
EaeI y'GGCC_r
Cuts at: 161 342 602 2081 2255 2646 2673 4018 161
Size: 181 260 1479 174 391 27 1345 639
Fragments arranged by size:
1479 1345 639 391 260 181 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 2704 3014 3160 4306 130
Size: 2574 310 146 1146 320
Fragments arranged by size:
2574 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 1446 1508 1446
Size: 62 4434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 508 678 807 892 1108 1411 1426 1432
Size: 394 170 129 85 216 303 15 6
Cuts at: 1432 1489 2321 2753 3500 3768 114
Size: 57 832 432 747 268 842
Fragments arranged by size:
842 832 747 432 394 303 268 216
170 129 85 57 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1183 1183
Size: 4496
EcoO109I rG'GnC_Cy
Cuts at: 278 450 767 1008 1191 1213 1265 1332 1635
Size: 172 317 241 183 22 52 67 303
Cuts at: 1635 278
Size: 3139
Fragments arranged by size:
3139 317 303 241 183 172 67 52
22
EcoRI G'AATT_C
Cuts at: 1500 1500
Size: 4496
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 709 2277 709
Size: 1568 2928
FspAI rTGC'GCAy
Cuts at: 709 709
Size: 4496
HaeII r_GCGC'y
Cuts at: 853 1458 1930 2178 3200 4179 853
Size: 605 472 248 1022 979 1170
Fragments arranged by size:
1170 1022 979 605 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 868 900 1149 1181 1381 1413 2338 2368 2370
Size: 32 249 32 200 32 925 30 2
Cuts at: 2370 2400 868
Size: 30 2964
Fragments arranged by size:
2964 925 249 200 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 234 1326 1535 234
Size: 1092 209 3195
Fragments arranged by size:
3195 1092 209
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 776 1154 3167 3351 776
Size: 378 2013 184 1921
Fragments arranged by size:
2013 1921 378 184
MscI TGG'CCA
Cuts at: 344 2257 344
Size: 1913 2583
MslI CAynn'nnrTG
Cuts at: 424 1153 1159 2485 2612 424
Size: 729 6 1326 127 2308
Fragments arranged by size:
2308 1326 729 127 6
MspA1I CmG'CkG
Cuts at: 269 882 911 1056 1607 1921 2281 3294 3539
Size: 613 29 145 551 314 360 1013 245
Cuts at: 3539 269
Size: 1226
Fragments arranged by size:
1226 1013 613 551 360 314 245 145
29
NaeI GCC'GGC
Cuts at: 115 161 2677 115
Size: 46 2516 1934
Fragments arranged by size:
2516 1934 46
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NgoMIV G'CCGG_C
Cuts at: 113 159 2675 113
Size: 46 2516 1934
Fragments arranged by size:
2516 1934 46
NspI r_CATG'y
Cuts at: 1074 1549 2049 2956 1074
Size: 475 500 907 2614
Fragments arranged by size:
2614 907 500 475
PasI CC'CwG_GG
Cuts at: 297 297
Size: 4496
PciI A'CATG_T
Cuts at: 2952 2952
Size: 4496
PfoI T'CCnGG_A
Cuts at: 1184 1184
Size: 4496
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PmlI CAC'GTG
Cuts at: 147 147
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 767 1191 767
Size: 424 4072
PspOMI G'GGCC_C
Cuts at: 618 1213 1264 618
Size: 595 51 3850
Fragments arranged by size:
3850 595 51
PstI C_TGCA'G
Cuts at: 893 1543 2228 893
Size: 650 685 3161
Fragments arranged by size:
3161 685 650
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1448 1510 1448
Size: 62 4434
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SanDI GG'GwC_CC
Cuts at: 767 767
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 893 1543 893
Size: 650 3846
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 645 889 1539 2224 3217 3408 33
Size: 612 244 650 685 993 191 1121
Fragments arranged by size:
1121 993 685 650 612 244 191
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 1282 1651 2566 3058 3320 1282
Size: 369 915 492 262 2458
Fragments arranged by size:
2458 915 492 369 262
SnaBI TAC'GTA
Cuts at: 1296 1296
Size: 4496
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StuI AGG'CCT
Cuts at: 674 674
Size: 4496
StyI C'CwwG_G
Cuts at: 164 1043 1487 1630 1984 164
Size: 879 444 143 354 2676
Fragments arranged by size:
2676 879 444 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 647 1218 2079 2212 2671 2880 647
Size: 571 861 133 459 209 2263
Fragments arranged by size:
2263 861 571 459 209 133
TatI w'GTAC_w
Cuts at: 4011 4011
Size: 4496
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1012 1044 4013 4045 1012
Size: 32 2969 32 1463
Fragments arranged by size:
2969 1463 32 32
Tth111I GACn'n_nGTC
Cuts at: 391 2293 391
Size: 1902 2594
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 612 1083 1991 612
Size: 471 908 3117
Fragments arranged by size:
3117 908 471
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SrfI SspI SwaI XhoI XmnI
ZraI