Restriction map for
pF1KB0993
(Circular) (Six-base) MAPSORT of: pF1KB0993.seq Check: 7275 from: 1 to: 3947
pF1KB0993 3947 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 985 985
Size: 3947
Acc65I G'GTAC_C
Cuts at: 963 963
Size: 3947
AclI AA'CG_TT
Cuts at: 3787 3787
Size: 3947
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 724 1772 2204 2951 3219 304
Size: 420 1048 432 747 268 1032
Fragments arranged by size:
1048 1032 747 432 420 268
AflIII A'CryG_T
Cuts at: 1299 2403 3380 1299
Size: 1104 977 1866
Fragments arranged by size:
1866 1104 977
AgeI A'CCGG_T
Cuts at: 2335 2335
Size: 3947
AlwNI CAG_nnn'CTG
Cuts at: 1252 2819 1252
Size: 1567 2380
ApaI G_GGCC'C
Cuts at: 202 202
Size: 3947
ApaLI G'TGCA_C
Cuts at: 2717 2717
Size: 3947
ApoI r'AATT_y
Cuts at: 679 951 3300 3556 679
Size: 272 2349 256 1070
Fragments arranged by size:
2349 1070 272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3947
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
AvaI C'yCGr_G
Cuts at: 201 880 967 201
Size: 679 87 3181
Fragments arranged by size:
3181 679 87
BamHI G'GATC_C
Cuts at: 972 972
Size: 3947
BanI G'GyrC_C
Cuts at: 963 1377 1625 1660 963
Size: 414 248 35 3250
Fragments arranged by size:
3250 414 248 35
BanII G_rGCy'C
Cuts at: 128 202 941 961 1991 128
Size: 74 739 20 1030 2084
Fragments arranged by size:
2084 1030 739 74 20
BbeI G_GCGC'C
Cuts at: 1381 1629 1381
Size: 248 3699
BbsI GAAGACnn'nnnn_
Cuts at: 133 674 133
Size: 541 3406
BbvCI CC'TCA_GC
Cuts at: 218 218
Size: 3947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 556 590 556
Size: 34 3913
BciVI GTATCCnnnnn_n'
Cuts at: 1845 2606 3947 1845
Size: 761 1341 1845
Fragments arranged by size:
1845 1341 761
BclI T'GATC_A
Cuts at: 1467 1467
Size: 3947
BglII A'GATC_T
Cuts at: 1462 3499 1462
Size: 2037 1910
BlpI GC'TnA_GC
Cuts at: 1059 1059
Size: 3947
Bme1580I G_kGCm'C
Cuts at: 202 1572 1665 2721 202
Size: 1370 93 1056 1428
Fragments arranged by size:
1428 1370 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 240 1159 1240 1575 3719 240
Size: 919 81 335 2144 468
Fragments arranged by size:
2144 919 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2314 2314
Size: 3947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 383
Size: 3947
Bpu10I CC'TnA_GC
Cuts at: 122 218 122
Size: 96 3851
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 883 1123 2002 2494 2792 577
Size: 306 240 879 492 298 1732
Fragments arranged by size:
1732 879 492 306 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 403 484 863 3651 403
Size: 81 379 2788 699
Fragments arranged by size:
2788 699 379 81
BsaAI yAC'GTr
Cuts at: 699 1300 699
Size: 601 3346
BsaBI GATnn'nnATC
Cuts at: 6 544 1461 3498 6
Size: 538 917 2037 455
Fragments arranged by size:
2037 917 538 455
BsaHI Gr'CG_yC
Cuts at: 1378 1626 2328 3824 1378
Size: 248 702 1496 1501
Fragments arranged by size:
1501 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1136 1657 2335 2609 2756 3167 1136
Size: 521 678 274 147 411 1916
Fragments arranged by size:
1916 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 271 301 271
Size: 30 3917
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 643 898 235
Size: 408 255 3284
Fragments arranged by size:
3284 408 255
BseYI C'CCAG_C
Cuts at: 195 244 852 874 1372 2130 2707 3203 195
Size: 49 608 22 498 758 577 496 939
Fragments arranged by size:
939 758 608 577 498 496 49 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3947
BsiEI CG_ry'CG
Cuts at: 104 1489 1535 2743 104
Size: 1385 46 1208 1308
Fragments arranged by size:
1385 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 941 961 1739 1929 2721 941
Size: 20 778 190 792 2167
Fragments arranged by size:
2167 792 778 190 20
Bsp1286I G_dGCh'C
Cuts at: 128 202 941 961 1572 1665 1739 1929 1991
Size: 74 739 20 611 93 74 190 62
Cuts at: 1991 2721 128
Size: 730 1354
Fragments arranged by size:
1354 739 730 611 190 93 74 74
62 20
BspEI T'CCGG_A
Cuts at: 3167 3167
Size: 3947
BspHI T'CATG_A
Cuts at: 3123 3123
Size: 3947
BspMI ACCTGCnnnn'nnnn_
Cuts at: 997 1211 1513 1894 997
Size: 214 302 381 3050
Fragments arranged by size:
3050 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2239 2293 3777 3942 50
Size: 2189 54 1484 165 55
Fragments arranged by size:
2189 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1859 1859
Size: 3947
BsrFI r'CCGG_y
Cuts at: 183 304 457 1945 2126 2335 183
Size: 121 153 1488 181 209 1795
Fragments arranged by size:
1795 1488 209 181 153 121
BssSI C'ACGA_G
Cuts at: 2218 2576 2218
Size: 358 3589
BstAPI GCAn_nnn'nTGC
Cuts at: 1324 1324
Size: 3947
BstBI TT'CG_AA
Cuts at: 2308 2308
Size: 3947
BstXI CCAn_nnnn'nTGG
Cuts at: 726 726
Size: 3947
BstYI r'GATC_y
Cuts at: 1 972 1439 1462 1797 2043 3055 3141 3153
Size: 971 467 23 335 246 1012 86 12
Cuts at: 3153 3499 1
Size: 346 449
Fragments arranged by size:
1012 971 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1897 2078 2241 1897
Size: 181 163 3603
Fragments arranged by size:
3603 181 163
BtsI GCAGTG_nn'
Cuts at: 607 742 1190 607
Size: 135 448 3364
Fragments arranged by size:
3364 448 135
ClaI AT'CG_AT
Cuts at: 468 468
Size: 3947
DraI TTT'AAA
Cuts at: 946 946
Size: 3947
DrdI GACnn_nn'nnGTC
Cuts at: 1653 2511 1653
Size: 858 3089
EaeI y'GGCC_r
Cuts at: 1532 1706 2097 2124 3469 1532
Size: 174 391 27 1345 2010
Fragments arranged by size:
2010 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1532 1532
Size: 3947
EarI CTCTTCn'nnn_
Cuts at: 614 787 1970 2180 614
Size: 173 1183 210 2381
Fragments arranged by size:
2381 1183 210 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2155 2465 2611 3757 2155
Size: 310 146 1146 2345
Fragments arranged by size:
2345 1146 310 146
EcoICRI GAG'CTC
Cuts at: 939 959 939
Size: 20 3927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 383 724 1772 2204 2951 3219 304
Size: 79 341 1048 432 747 268 1032
Fragments arranged by size:
1048 1032 747 432 341 268 79
EcoNI CCTnn'n_nnAGG
Cuts at: 445 445
Size: 3947
EcoO109I rG'GnC_Cy
Cuts at: 1086 1086
Size: 3947
EcoRI G'AATT_C
Cuts at: 679 951 679
Size: 272 3675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 309 402 1966 309
Size: 93 1564 2290
Fragments arranged by size:
2290 1564 93
FspI TGC'GCA
Cuts at: 1728 1728
Size: 3947
HaeII r_GCGC'y
Cuts at: 1381 1629 2651 3630 1381
Size: 248 1022 979 1698
Fragments arranged by size:
1698 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 271 303 724 756 1789 1819 1821
Size: 32 124 32 421 32 1033 30 2
Cuts at: 1821 1851 115
Size: 30 2211
Fragments arranged by size:
2211 1033 421 124 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 986 986
Size: 3947
KasI G'GCGC_C
Cuts at: 1377 1625 1377
Size: 248 3699
KpnI G_GTAC'C
Cuts at: 967 967
Size: 3947
MluI A'CGCG_T
Cuts at: 3380 3380
Size: 3947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 362 2618 2802 362
Size: 2256 184 1507
Fragments arranged by size:
2256 1507 184
MscI TGG'CCA
Cuts at: 1708 1708
Size: 3947
MslI CAynn'nnrTG
Cuts at: 1936 2063 1936
Size: 127 3820
MspA1I CmG'CkG
Cuts at: 137 763 1058 1372 1732 2745 2990 137
Size: 626 295 314 360 1013 245 1094
Fragments arranged by size:
1094 1013 626 360 314 295 245
NaeI GCC'GGC
Cuts at: 185 2128 185
Size: 1943 2004
NarI GG'CG_CC
Cuts at: 1378 1626 1378
Size: 248 3699
NcoI C'CATG_G
Cuts at: 107 107
Size: 3947
NgoMIV G'CCGG_C
Cuts at: 183 2126 183
Size: 1943 2004
NspI r_CATG'y
Cuts at: 486 1000 1500 2407 486
Size: 514 500 907 2026
Fragments arranged by size:
2026 907 514 500
PasI CC'CwG_GG
Cuts at: 768 768
Size: 3947
PciI A'CATG_T
Cuts at: 2403 2403
Size: 3947
PfoI T'CCnGG_A
Cuts at: 344 428 344
Size: 84 3863
PmeI GTTT'AAAC
Cuts at: 946 946
Size: 3947
PmlI CAC'GTG
Cuts at: 699 699
Size: 3947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 3915
PspOMI G'GGCC_C
Cuts at: 198 198
Size: 3947
PstI C_TGCA'G
Cuts at: 579 994 1679 579
Size: 415 685 2847
Fragments arranged by size:
2847 685 415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3947
PvuII CAG'CTG
Cuts at: 763 1372 1732 763
Size: 609 360 2978
Fragments arranged by size:
2978 609 360
RsrII CG'GwC_CG
Cuts at: 2142 2142
Size: 3947
SacI G_AGCT'C
Cuts at: 941 961 941
Size: 20 3927
SalI G'TCGA_C
Cuts at: 984 984
Size: 3947
SapI GCTCTTCn'nnn_
Cuts at: 787 1970 2180 787
Size: 1183 210 2554
Fragments arranged by size:
2554 1183 210
SbfI CC_TGCA'GG
Cuts at: 994 994
Size: 3947
ScaI AGT'ACT
Cuts at: 3464 3464
Size: 3947
SexAI A'CCwGG_T
Cuts at: 512 512
Size: 3947
SfcI C'TryA_G
Cuts at: 33 575 990 1675 2668 2859 33
Size: 542 415 685 993 191 1121
Fragments arranged by size:
1121 993 685 542 415 191
SfoI GGC'GCC
Cuts at: 1379 1627 1379
Size: 248 3699
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3947
SmaI CCC'GGG
Cuts at: 969 969
Size: 3947
SmlI C'TyrA_G
Cuts at: 592 862 1102 2017 2509 2771 592
Size: 270 240 915 492 262 1768
Fragments arranged by size:
1768 915 492 270 262 240
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3947
SphI G_CATG'C
Cuts at: 1000 1500 1000
Size: 500 3447
StuI AGG'CCT
Cuts at: 358 358
Size: 3947
StyI C'CwwG_G
Cuts at: 107 314 476 806 1081 1435 107
Size: 207 162 330 275 354 2619
Fragments arranged by size:
2619 354 330 275 207 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 844 1530 1663 2122 2331 844
Size: 686 133 459 209 2460
Fragments arranged by size:
2460 686 459 209 133
TatI w'GTAC_w
Cuts at: 3462 3462
Size: 3947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 3915
Tth111I GACn'n_nGTC
Cuts at: 1744 1744
Size: 3947
XbaI T'CTAG_A
Cuts at: 978 978
Size: 3947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 358 1442 358
Size: 1084 2863
XmaI C'CCGG_G
Cuts at: 967 967
Size: 3947
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI