Restriction map for
pF1KB0983
(Circular) (Six-base) MAPSORT of: pF1KB0983.seq Check: 9690 from: 1 to: 3833
pF1KB0983 3833 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 871 871
Size: 3833
Acc65I G'GTAC_C
Cuts at: 390 849 390
Size: 459 3374
AclI AA'CG_TT
Cuts at: 3673 3673
Size: 3833
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1658 2090 2837 3105 1658
Size: 432 747 268 2386
Fragments arranged by size:
2386 747 432 268
AflIII A'CryG_T
Cuts at: 1185 2289 3266 1185
Size: 1104 977 1752
Fragments arranged by size:
1752 1104 977
AgeI A'CCGG_T
Cuts at: 2221 2221
Size: 3833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 3833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 325 357 325
Size: 32 3801
AlwNI CAG_nnn'CTG
Cuts at: 496 565 1138 2705 496
Size: 69 573 1567 1624
Fragments arranged by size:
1624 1567 573 69
ApaI G_GGCC'C
Cuts at: 709 709
Size: 3833
ApaLI G'TGCA_C
Cuts at: 2603 2603
Size: 3833
ApoI r'AATT_y
Cuts at: 222 837 3186 3442 222
Size: 615 2349 256 613
Fragments arranged by size:
2349 615 613 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3833
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
AvaI C'yCGr_G
Cuts at: 386 731 853 386
Size: 345 122 3366
Fragments arranged by size:
3366 345 122
BamHI G'GATC_C
Cuts at: 858 858
Size: 3833
BanI G'GyrC_C
Cuts at: 193 390 849 1263 1511 1546 193
Size: 197 459 414 248 35 2480
Fragments arranged by size:
2480 459 414 248 197 35
BanII G_rGCy'C
Cuts at: 600 709 847 1877 600
Size: 109 138 1030 2556
Fragments arranged by size:
2556 1030 138 109
BbeI G_GCGC'C
Cuts at: 1267 1515 1267
Size: 248 3585
BciVI GTATCCnnnnn_n'
Cuts at: 175 351 1731 2492 3833 175
Size: 176 1380 761 1341 175
Fragments arranged by size:
1380 1341 761 176 175
BclI T'GATC_A
Cuts at: 1353 1353
Size: 3833
BglII A'GATC_T
Cuts at: 1348 3385 1348
Size: 2037 1796
BlpI GC'TnA_GC
Cuts at: 945 945
Size: 3833
Bme1580I G_kGCm'C
Cuts at: 628 709 1458 1551 2607 628
Size: 81 749 93 1056 1854
Fragments arranged by size:
1854 1056 749 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 1045 1126 1461 3605 1045
Size: 81 335 2144 1273
Fragments arranged by size:
2144 1273 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 791 2200 791
Size: 1409 2424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 431 614 647 422
Size: 9 183 33 3608
Fragments arranged by size:
3608 183 33 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1888 2380 2678 1009
Size: 879 492 298 2164
Fragments arranged by size:
2164 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 687 762 3537 687
Size: 75 2775 983
Fragments arranged by size:
2775 983 75
BsaAI yAC'GTr
Cuts at: 1186 1186
Size: 3833
BsaBI GATnn'nnATC
Cuts at: 6 1347 3384 6
Size: 1341 2037 455
Fragments arranged by size:
2037 1341 455
BsaHI Gr'CG_yC
Cuts at: 739 1264 1512 2214 3710 739
Size: 525 248 702 1496 862
Fragments arranged by size:
1496 862 702 525 248
BsaWI w'CCGG_w
Cuts at: 1022 1543 2221 2495 2642 3053 1022
Size: 521 678 274 147 411 1802
Fragments arranged by size:
1802 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 645 675 645
Size: 30 3803
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 458 518 668 458
Size: 60 150 3623
Fragments arranged by size:
3623 150 60
BseYI C'CCAG_C
Cuts at: 521 1258 2016 2593 3089 521
Size: 737 758 577 496 1265
Fragments arranged by size:
1265 758 737 577 496
BsiEI CG_ry'CG
Cuts at: 104 1375 1421 2629 104
Size: 1271 46 1208 1308
Fragments arranged by size:
1308 1271 1208 46
BsiHKAI G_wGCw'C
Cuts at: 847 1625 1815 2607 847
Size: 778 190 792 2073
Fragments arranged by size:
2073 792 778 190
BsmI GAATG_Cn'
Cuts at: 276 815 276
Size: 539 3294
Bsp1286I G_dGCh'C
Cuts at: 600 628 709 847 1458 1551 1625 1815 1877
Size: 28 81 138 611 93 74 190 62
Cuts at: 1877 2607 600
Size: 730 1826
Fragments arranged by size:
1826 730 611 190 138 93 81 74
62 28
BspEI T'CCGG_A
Cuts at: 3053 3053
Size: 3833
BspHI T'CATG_A
Cuts at: 3009 3009
Size: 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 883 1097 1399 1780 516
Size: 367 214 302 381 2569
Fragments arranged by size:
2569 381 367 302 214
BsrBI CCG'CTC
Cuts at: 50 2125 2179 3663 3828 50
Size: 2075 54 1484 165 55
Fragments arranged by size:
2075 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 380 1745 380
Size: 1365 2468
BsrFI r'CCGG_y
Cuts at: 1831 2012 2221 1831
Size: 181 209 3443
Fragments arranged by size:
3443 209 181
BssSI C'ACGA_G
Cuts at: 2104 2462 2104
Size: 358 3475
BstAPI GCAn_nnn'nTGC
Cuts at: 565 1210 565
Size: 645 3188
BstBI TT'CG_AA
Cuts at: 2194 2194
Size: 3833
BstXI CCAn_nnnn'nTGG
Cuts at: 351 351
Size: 3833
BstYI r'GATC_y
Cuts at: 1 858 1325 1348 1683 1929 2941 3027 3039
Size: 857 467 23 335 246 1012 86 12
Cuts at: 3039 3385 1
Size: 346 449
Fragments arranged by size:
1012 857 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 701 701
Size: 3833
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1783 1964 2127 1783
Size: 181 163 3489
Fragments arranged by size:
3489 181 163
BtsI GCAGTG_nn'
Cuts at: 1076 1076
Size: 3833
DraI TTT'AAA
Cuts at: 832 832
Size: 3833
DrdI GACnn_nn'nnGTC
Cuts at: 1539 2397 1539
Size: 858 2975
EaeI y'GGCC_r
Cuts at: 177 1418 1592 1983 2010 3355 177
Size: 1241 174 391 27 1345 655
Fragments arranged by size:
1345 1241 655 391 174 27
EagI C'GGCC_G
Cuts at: 1418 1418
Size: 3833
EarI CTCTTCn'nnn_
Cuts at: 540 1856 2066 540
Size: 1316 210 2307
Fragments arranged by size:
2307 1316 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 620 2041 2351 2497 3643 620
Size: 1421 310 146 1146 810
Fragments arranged by size:
1421 1146 810 310 146
EcoICRI GAG'CTC
Cuts at: 845 845
Size: 3833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 431 614 647 1658 2090 2837 3105 422
Size: 9 183 33 1011 432 747 268 1150
Fragments arranged by size:
1150 1011 747 432 268 183 33 9
EcoNI CCTnn'n_nnAGG
Cuts at: 500 731 779 803 500
Size: 231 48 24 3530
Fragments arranged by size:
3530 231 48 24
EcoO109I rG'GnC_Cy
Cuts at: 415 474 493 972 415
Size: 59 19 479 3276
Fragments arranged by size:
3276 479 59 19
EcoRI G'AATT_C
Cuts at: 837 837
Size: 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1852 1852
Size: 3833
FspI TGC'GCA
Cuts at: 1614 1614
Size: 3833
HaeII r_GCGC'y
Cuts at: 1267 1515 2537 3516 1267
Size: 248 1022 979 1584
Fragments arranged by size:
1584 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1675 1705 1707 1737 1675
Size: 30 2 30 3771
Fragments arranged by size:
3771 30 30 2
HincII GTy'rAC
Cuts at: 872 872
Size: 3833
KasI G'GCGC_C
Cuts at: 1263 1511 1263
Size: 248 3585
KpnI G_GTAC'C
Cuts at: 394 853 394
Size: 459 3374
MfeI C'AATT_G
Cuts at: 713 713
Size: 3833
MluI A'CGCG_T
Cuts at: 3266 3266
Size: 3833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2688 2504
Size: 184 3649
MscI TGG'CCA
Cuts at: 1594 1594
Size: 3833
MslI CAynn'nnrTG
Cuts at: 349 1822 1949 349
Size: 1473 127 2233
Fragments arranged by size:
2233 1473 127
MspA1I CmG'CkG
Cuts at: 436 454 562 944 1258 1618 2631 2876 436
Size: 18 108 382 314 360 1013 245 1393
Fragments arranged by size:
1393 1013 382 360 314 245 108 18
NaeI GCC'GGC
Cuts at: 2014 2014
Size: 3833
NarI GG'CG_CC
Cuts at: 1264 1512 1264
Size: 248 3585
NcoI C'CATG_G
Cuts at: 701 701
Size: 3833
NdeI CA'TA_TG
Cuts at: 218 218
Size: 3833
NgoMIV G'CCGG_C
Cuts at: 2012 2012
Size: 3833
NspI r_CATG'y
Cuts at: 753 886 1386 2293 753
Size: 133 500 907 2293
Fragments arranged by size:
2293 907 500 133
PasI CC'CwG_GG
Cuts at: 418 418
Size: 3833
PciI A'CATG_T
Cuts at: 2289 2289
Size: 3833
PflMI CCAn_nnn'nTGG
Cuts at: 400 715 400
Size: 315 3518
PfoI T'CCnGG_A
Cuts at: 759 759
Size: 3833
PmeI GTTT'AAAC
Cuts at: 832 832
Size: 3833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2972 3004 2972
Size: 32 3801
PspOMI G'GGCC_C
Cuts at: 705 705
Size: 3833
PspXI vC'TCGA_Gb
Cuts at: 731 731
Size: 3833
PstI C_TGCA'G
Cuts at: 140 880 1565 140
Size: 740 685 2408
Fragments arranged by size:
2408 740 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3833
PvuII CAG'CTG
Cuts at: 436 454 562 1258 1618 436
Size: 18 108 696 360 2651
Fragments arranged by size:
2651 696 360 108 18
RsrII CG'GwC_CG
Cuts at: 2028 2028
Size: 3833
SacI G_AGCT'C
Cuts at: 847 847
Size: 3833
SalI G'TCGA_C
Cuts at: 870 870
Size: 3833
SapI GCTCTTCn'nnn_
Cuts at: 1856 2066 1856
Size: 210 3623
SbfI CC_TGCA'GG
Cuts at: 880 880
Size: 3833
ScaI AGT'ACT
Cuts at: 3350 3350
Size: 3833
SexAI A'CCwGG_T
Cuts at: 684 684
Size: 3833
SfcI C'TryA_G
Cuts at: 33 136 150 554 876 1561 2554 2745 33
Size: 103 14 404 322 685 993 191 1121
Fragments arranged by size:
1121 993 685 404 322 191 103 14
SfoI GGC'GCC
Cuts at: 1265 1513 1265
Size: 248 3585
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3833
SmaI CCC'GGG
Cuts at: 855 855
Size: 3833
SmlI C'TyrA_G
Cuts at: 731 988 1903 2395 2657 731
Size: 257 915 492 262 1907
Fragments arranged by size:
1907 915 492 262 257
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3833
SphI G_CATG'C
Cuts at: 753 886 1386 753
Size: 133 500 3200
Fragments arranged by size:
3200 500 133
StyI C'CwwG_G
Cuts at: 701 967 1321 701
Size: 266 354 3213
Fragments arranged by size:
3213 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 714 1416 1549 2008 2217 714
Size: 702 133 459 209 2330
Fragments arranged by size:
2330 702 459 209 133
TatI w'GTAC_w
Cuts at: 3348 3348
Size: 3833
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 3801
Tth111I GACn'n_nGTC
Cuts at: 1630 1630
Size: 3833
XbaI T'CTAG_A
Cuts at: 864 864
Size: 3833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 478 1328 478
Size: 850 2983
XhoI C'TCGA_G
Cuts at: 731 731
Size: 3833
XmaI C'CCGG_G
Cuts at: 853 853
Size: 3833
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI NheI NotI NruI NsiI PacI PmlI PpuMI
PshAI PsiI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XmnI ZraI