Restriction map for
pF1KB0966
(Circular) (Six-base) MAPSORT of: pF1KB0966.seq Check: 6611 from: 1 to: 4394
pF1KB0966 4394 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 813 981 2219 2651 3398 3666 729
Size: 84 168 1238 432 747 268 1457
Fragments arranged by size:
1457 1238 747 432 268 168 84
AflII C'TTAA_G
Cuts at: 423 1338 423
Size: 915 3479
AflIII A'CryG_T
Cuts at: 213 343 505 1746 2850 3827 213
Size: 130 162 1241 1104 977 780
Fragments arranged by size:
1241 1104 977 780 162 130
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AleI CACnn'nnGTG
Cuts at: 344 1208 344
Size: 864 3530
AlwNI CAG_nnn'CTG
Cuts at: 145 1699 3266 145
Size: 1554 1567 1273
Fragments arranged by size:
1567 1554 1273
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 695 1115 1159 1398 3747 4003 695
Size: 420 44 239 2349 256 1086
Fragments arranged by size:
2349 1086 420 256 239 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1414 1414
Size: 4394
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 1358 1410 1824 2072 2107 1358
Size: 52 414 248 35 3645
Fragments arranged by size:
3645 414 248 52 35
BanII G_rGCy'C
Cuts at: 1408 2438 1408
Size: 1030 3364
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BbsI GAAGACnn'nnnn_
Cuts at: 586 987 586
Size: 401 3993
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BfrBI ATG'CAT
Cuts at: 610 630 610
Size: 20 4374
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 2019 2112 3168 2019
Size: 93 1056 3245
Fragments arranged by size:
3245 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 809 893 1061 1313 809
Size: 84 168 252 3890
Fragments arranged by size:
3890 252 168 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2449 2941 3239 1570
Size: 879 492 298 2725
Fragments arranged by size:
2725 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4098 4098
Size: 4394
BsaAI yAC'GTr
Cuts at: 506 1747 506
Size: 1241 3153
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1583 2104 2782 3056 3203 3614 1583
Size: 521 678 274 147 411 2363
Fragments arranged by size:
2363 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 529 529
Size: 4394
BseYI C'CCAG_C
Cuts at: 1819 2577 3154 3650 1819
Size: 758 577 496 2563
Fragments arranged by size:
2563 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 660 744 996 1248 1332 447
Size: 213 84 252 252 84 3509
Fragments arranged by size:
3509 252 252 213 84 84
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 595 1408 2186 2376 3168 595
Size: 813 778 190 792 1821
Fragments arranged by size:
1821 813 792 778 190
BsmI GAATG_Cn'
Cuts at: 811 1156 811
Size: 345 4049
Bsp1286I G_dGCh'C
Cuts at: 595 1408 2019 2112 2186 2376 2438 3168 595
Size: 813 611 93 74 190 62 730 1821
Fragments arranged by size:
1821 813 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 223 1444 1658 1960 2341 223
Size: 1221 214 302 381 2276
Fragments arranged by size:
2276 1221 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 900 2306 900
Size: 1406 2988
BsrFI r'CCGG_y
Cuts at: 2392 2573 2782 2392
Size: 181 209 4004
Fragments arranged by size:
4004 209 181
BsrGI T'GTAC_A
Cuts at: 936 936
Size: 4394
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1151 1771 1151
Size: 620 3774
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstYI r'GATC_y
Cuts at: 1 1419 1886 1909 2244 2490 3502 3588 3600
Size: 1418 467 23 335 246 1012 86 12
Cuts at: 3600 3946 1
Size: 346 449
Fragments arranged by size:
1418 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 324 324
Size: 4394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 212 648 984 1068 1320 1373 1637 212
Size: 436 336 84 252 53 264 2969
Fragments arranged by size:
2969 436 336 264 252 84 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 882 917 882
Size: 35 4359
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 679 2100 2958 679
Size: 1421 858 2115
Fragments arranged by size:
2115 1421 858
EaeI y'GGCC_r
Cuts at: 1083 1979 2153 2544 2571 3916 1083
Size: 896 174 391 27 1345 1561
Fragments arranged by size:
1561 1345 896 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 167 2417 2627 167
Size: 2250 210 1934
Fragments arranged by size:
2250 1934 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 809 813 893 981 1061 1313 2219 2651
Size: 80 4 80 88 80 252 906 432
Cuts at: 2651 3398 3666 729
Size: 747 268 1457
Fragments arranged by size:
1457 906 747 432 268 252 88 80
80 80 4
EcoNI CCTnn'n_nnAGG
Cuts at: 426 426
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 142 1533 142
Size: 1391 3003
EcoRI G'AATT_C
Cuts at: 695 1115 1398 695
Size: 420 283 3691
Fragments arranged by size:
3691 420 283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 694 778 862 2413 694
Size: 84 84 1551 2675
Fragments arranged by size:
2675 1551 84 84
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 2236 2266 2268 2298 160
Size: 32 2044 30 2 30 2256
Fragments arranged by size:
2256 2044 32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
HindIII A'AGCT_T
Cuts at: 656 656
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MfeI C'AATT_G
Cuts at: 449 449
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3065 3249 3065
Size: 184 4210
MscI TGG'CCA
Cuts at: 1085 2155 1085
Size: 1070 3324
MslI CAynn'nnrTG
Cuts at: 302 344 1208 1308 2383 2510 302
Size: 42 864 100 1075 127 2186
Fragments arranged by size:
2186 1075 864 127 100 42
MspA1I CmG'CkG
Cuts at: 1505 1819 2179 3192 3437 1505
Size: 314 360 1013 245 2462
Fragments arranged by size:
2462 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 324 324
Size: 4394
NdeI CA'TA_TG
Cuts at: 268 1305 268
Size: 1037 3357
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NsiI A_TGCA'T
Cuts at: 612 632 612
Size: 20 4374
NspI r_CATG'y
Cuts at: 217 347 356 1447 1947 2854 217
Size: 130 9 1091 500 907 1757
Fragments arranged by size:
1757 1091 907 500 130 9
PciI A'CATG_T
Cuts at: 213 343 2850 213
Size: 130 2507 1757
Fragments arranged by size:
2507 1757 130
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PshAI GACnn'nnGTC
Cuts at: 115 115
Size: 4394
PsiI TTA'TAA
Cuts at: 887 971 887
Size: 84 4310
PstI C_TGCA'G
Cuts at: 1380 1441 2126 1380
Size: 61 685 3648
Fragments arranged by size:
3648 685 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1819 2179 1819
Size: 360 4034
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 200 1332 1376 1437 2122 3115 3306 33
Size: 167 1132 44 61 685 993 191 1121
Fragments arranged by size:
1132 1121 993 685 191 167 61 44
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 423 1338 1549 2464 2956 3218 423
Size: 915 211 915 492 262 1599
Fragments arranged by size:
1599 915 915 492 262 211
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
SspI AAT'ATT
Cuts at: 910 910
Size: 4394
StuI AGG'CCT
Cuts at: 445 798 966 1218 1330 445
Size: 353 168 252 112 3509
Fragments arranged by size:
3509 353 252 168 112
StyI C'CwwG_G
Cuts at: 227 324 1528 1882 227
Size: 97 1204 354 2739
Fragments arranged by size:
2739 1204 354 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 532 1977 2110 2569 2778 532
Size: 1445 133 459 209 2148
Fragments arranged by size:
2148 1445 459 209 133
TatI w'GTAC_w
Cuts at: 936 3909 936
Size: 2973 1421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
XmnI GAAnn'nnTTC
Cuts at: 526 658 742 994 526
Size: 132 84 252 3926
Fragments arranged by size:
3926 252 132 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PciI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HpaI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI