Restriction map for
pF1KB0934
(Circular) (Six-base) MAPSORT of: pF1KB0934.seq Check: 7258 from: 1 to: 4256
pF1KB0934 4256 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 152 908 1294 152
Size: 756 386 3114
Fragments arranged by size:
3114 756 386
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1106 2081 2513 3260 3528 311
Size: 795 975 432 747 268 1039
Fragments arranged by size:
1039 975 795 747 432 268
AflII C'TTAA_G
Cuts at: 775 775
Size: 4256
AflIII A'CryG_T
Cuts at: 1608 2712 3689 1608
Size: 1104 977 2175
Fragments arranged by size:
2175 1104 977
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 988 1561 3128 988
Size: 573 1567 2116
Fragments arranged by size:
2116 1567 573
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 589 631 721 796 1260 3609 3865 589
Size: 42 90 75 464 2349 256 980
Fragments arranged by size:
2349 980 464 256 90 75 42
AseI AT'TA_AT
Cuts at: 20 1181 20
Size: 1161 3095
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 1276 1276
Size: 4256
BamHI G'GATC_C
Cuts at: 505 1281 505
Size: 776 3480
BanI G'GyrC_C
Cuts at: 1272 1686 1934 1969 1272
Size: 414 248 35 3559
Fragments arranged by size:
3559 414 248 35
BanII G_rGCy'C
Cuts at: 1270 2300 1270
Size: 1030 3226
BbeI G_GCGC'C
Cuts at: 1690 1938 1690
Size: 248 4008
BbsI GAAGACnn'nnnn_
Cuts at: 408 631 1142 408
Size: 223 511 3522
Fragments arranged by size:
3522 511 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 369 403 90
Size: 34 245 34 3943
Fragments arranged by size:
3943 245 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 445 2154 2915 4256 445
Size: 1709 761 1341 445
Fragments arranged by size:
1709 1341 761 445
BclI T'GATC_A
Cuts at: 713 1776 713
Size: 1063 3193
BfrBI ATG'CAT
Cuts at: 238 238
Size: 4256
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 1068 1881 1974 3030 1068
Size: 813 93 1056 2294
Fragments arranged by size:
2294 1056 813 93
BmrI ACTGGGnnnn_n'
Cuts at: 1062 1468 1549 1884 4028 1062
Size: 406 81 335 2144 1290
Fragments arranged by size:
2144 1290 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 880 2623 880
Size: 1743 2513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 1432 2311 2803 3101 956
Size: 476 879 492 298 2111
Fragments arranged by size:
2111 879 492 476 298
BsaI GGTCTCn'nnnn_
Cuts at: 1038 3960 1038
Size: 2922 1334
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 1687 1935 2637 4133 1687
Size: 248 702 1496 1810
Fragments arranged by size:
1810 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1681 2439 3016 3512 1681
Size: 758 577 496 2425
Fragments arranged by size:
2425 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1798 1844 3052 104
Size: 1694 46 1208 1308
Fragments arranged by size:
1694 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1270 2048 2238 3030 1270
Size: 778 190 792 2496
Fragments arranged by size:
2496 792 778 190
BsmI GAATG_Cn'
Cuts at: 181 336 181
Size: 155 4101
Bsp1286I G_dGCh'C
Cuts at: 1068 1270 1881 1974 2048 2238 2300 3030 1068
Size: 202 611 93 74 190 62 730 2294
Fragments arranged by size:
2294 730 611 202 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 710 3432 710
Size: 2722 1534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1306 1520 1822 2203 1306
Size: 214 302 381 3359
Fragments arranged by size:
3359 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1097 2548 2602 4086 4251 50
Size: 1047 1451 54 1484 165 55
Fragments arranged by size:
1484 1451 1047 165 55 54
BsrDI GCAATG_nn'
Cuts at: 894 2168 894
Size: 1274 2982
BsrFI r'CCGG_y
Cuts at: 2254 2435 2644 2254
Size: 181 209 3866
Fragments arranged by size:
3866 209 181
BsrGI T'GTAC_A
Cuts at: 789 789
Size: 4256
BssSI C'ACGA_G
Cuts at: 2527 2885 2527
Size: 358 3898
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 837 1075 837
Size: 238 4018
BstYI r'GATC_y
Cuts at: 1 505 1281 1748 1771 2106 2352 3364 3450
Size: 504 776 467 23 335 246 1012 86
Cuts at: 3450 3462 3808 1
Size: 12 346 449
Fragments arranged by size:
1012 776 504 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 1499 1499
Size: 4256
DraI TTT'AAA
Cuts at: 299 434 1255 299
Size: 135 821 3300
Fragments arranged by size:
3300 821 135
DrdI GACnn_nn'nnGTC
Cuts at: 1962 2820 1962
Size: 858 3398
EaeI y'GGCC_r
Cuts at: 1841 2015 2406 2433 3778 1841
Size: 174 391 27 1345 2319
Fragments arranged by size:
2319 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 2774 2920 4066 2464
Size: 310 146 1146 2654
Fragments arranged by size:
2654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 1106 2081 2513 3260 3528 311
Size: 795 975 432 747 268 1039
Fragments arranged by size:
1039 975 795 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 971 971
Size: 4256
EcoO109I rG'GnC_Cy
Cuts at: 1395 1395
Size: 4256
EcoRI G'AATT_C
Cuts at: 589 1260 589
Size: 671 3585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 790 2275 790
Size: 1485 2771
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 1690 1938 2960 3939 1690
Size: 248 1022 979 2007
Fragments arranged by size:
2007 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 529 561 970 1002 2098 2128 2130
Size: 32 139 32 409 32 1096 30 2
Cuts at: 2130 2160 358
Size: 30 2454
Fragments arranged by size:
2454 1096 409 139 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1295 1295
Size: 4256
KasI G'GCGC_C
Cuts at: 1686 1934 1686
Size: 248 4008
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 2927 3111 533
Size: 2394 184 1678
Fragments arranged by size:
2394 1678 184
MscI TGG'CCA
Cuts at: 2017 2017
Size: 4256
MslI CAynn'nnrTG
Cuts at: 124 499 709 2245 2372 124
Size: 375 210 1536 127 2008
Fragments arranged by size:
2008 1536 375 210 127
MspA1I CmG'CkG
Cuts at: 289 1367 1681 2041 3054 3299 289
Size: 1078 314 360 1013 245 1246
Fragments arranged by size:
1246 1078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2437 2437
Size: 4256
NarI GG'CG_CC
Cuts at: 1687 1935 1687
Size: 248 4008
NdeI CA'TA_TG
Cuts at: 597 597
Size: 4256
NgoMIV G'CCGG_C
Cuts at: 2435 2435
Size: 4256
NsiI A_TGCA'T
Cuts at: 240 240
Size: 4256
NspI r_CATG'y
Cuts at: 1309 1809 2716 1309
Size: 500 907 2849
Fragments arranged by size:
2849 907 500
PciI A'CATG_T
Cuts at: 2712 2712
Size: 4256
PfoI T'CCnGG_A
Cuts at: 728 728
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PshAI GACnn'nnGTC
Cuts at: 1001 1001
Size: 4256
PstI C_TGCA'G
Cuts at: 1303 1988 1303
Size: 685 3571
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 289 1681 2041 289
Size: 1392 360 2504
Fragments arranged by size:
2504 1392 360
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 2279 2489 2279
Size: 210 4046
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SfcI C'TryA_G
Cuts at: 33 200 1299 1984 2977 3168 33
Size: 167 1099 685 993 191 1121
Fragments arranged by size:
1121 1099 993 685 191 167
SfoI GGC'GCC
Cuts at: 1688 1936 1688
Size: 248 4008
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 1278 1278
Size: 4256
SmlI C'TyrA_G
Cuts at: 775 971 1411 2326 2818 3080 775
Size: 196 440 915 492 262 1951
Fragments arranged by size:
1951 915 492 440 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 913 1390 1744 913
Size: 477 354 3425
Fragments arranged by size:
3425 477 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1839 1972 2431 2640 1839
Size: 133 459 209 3455
Fragments arranged by size:
3455 459 209 133
TatI w'GTAC_w
Cuts at: 789 3771 789
Size: 2982 1274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 4224
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1751 1751
Size: 4256
XmaI C'CCGG_G
Cuts at: 1276 1276
Size: 4256
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI