Restriction map for
pF1KB0929
(Circular) (Six-base) MAPSORT of: pF1KB0929.seq Check: 5627 from: 1 to: 3521
pF1KB0929 3521 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 559 559
Size: 3521
Acc65I G'GTAC_C
Cuts at: 537 537
Size: 3521
AclI AA'CG_TT
Cuts at: 3361 3361
Size: 3521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1778 2525 2793 1346
Size: 432 747 268 2074
Fragments arranged by size:
2074 747 432 268
AflIII A'CryG_T
Cuts at: 456 873 1977 2954 456
Size: 417 1104 977 1023
Fragments arranged by size:
1104 1023 977 417
AgeI A'CCGG_T
Cuts at: 142 1909 142
Size: 1767 1754
AlwNI CAG_nnn'CTG
Cuts at: 318 826 2393 318
Size: 508 1567 1446
Fragments arranged by size:
1567 1446 508
ApaLI G'TGCA_C
Cuts at: 2291 2291
Size: 3521
ApoI r'AATT_y
Cuts at: 525 2874 3130 525
Size: 2349 256 916
Fragments arranged by size:
2349 916 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3521
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
AvaI C'yCGr_G
Cuts at: 541 541
Size: 3521
BamHI G'GATC_C
Cuts at: 546 546
Size: 3521
BanI G'GyrC_C
Cuts at: 537 951 1199 1234 537
Size: 414 248 35 2824
Fragments arranged by size:
2824 414 248 35
BanII G_rGCy'C
Cuts at: 535 1565 535
Size: 1030 2491
BbeI G_GCGC'C
Cuts at: 955 1203 955
Size: 248 3273
BbsI GAAGACnn'nnnn_
Cuts at: 431 431
Size: 3521
BciVI GTATCCnnnnn_n'
Cuts at: 1419 2180 3521 1419
Size: 761 1341 1419
Fragments arranged by size:
1419 1341 761
BclI T'GATC_A
Cuts at: 1041 1041
Size: 3521
BglII A'GATC_T
Cuts at: 351 1036 3073 351
Size: 685 2037 799
Fragments arranged by size:
2037 799 685
BlpI GC'TnA_GC
Cuts at: 633 633
Size: 3521
Bme1580I G_kGCm'C
Cuts at: 1146 1239 2295 1146
Size: 93 1056 2372
Fragments arranged by size:
2372 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 733 814 1149 3293 733
Size: 81 335 2144 961
Fragments arranged by size:
2144 961 335 81
BmtI G_CTAG'C
Cuts at: 490 490
Size: 3521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 227
Size: 3521
Bpu10I CC'TnA_GC
Cuts at: 335 335
Size: 3521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 1576 2068 2366 697
Size: 879 492 298 1852
Fragments arranged by size:
1852 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3225 3225
Size: 3521
BsaAI yAC'GTr
Cuts at: 502 874 502
Size: 372 3149
BsaBI GATnn'nnATC
Cuts at: 6 1035 3072 6
Size: 1029 2037 455
Fragments arranged by size:
2037 1029 455
BsaHI Gr'CG_yC
Cuts at: 952 1200 1902 3398 952
Size: 248 702 1496 1075
Fragments arranged by size:
1496 1075 702 248
BsaWI w'CCGG_w
Cuts at: 142 710 1231 1909 2183 2330 2741 142
Size: 568 521 678 274 147 411 922
Fragments arranged by size:
922 678 568 521 411 274 147
BseYI C'CCAG_C
Cuts at: 946 1704 2281 2777 946
Size: 758 577 496 1690
Fragments arranged by size:
1690 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 143 1063 1109 2317 104
Size: 39 920 46 1208 1308
Fragments arranged by size:
1308 1208 920 46 39
BsiHKAI G_wGCw'C
Cuts at: 535 1313 1503 2295 535
Size: 778 190 792 1761
Fragments arranged by size:
1761 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 535 1146 1239 1313 1503 1565 2295 535
Size: 611 93 74 190 62 730 1761
Fragments arranged by size:
1761 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2741 2741
Size: 3521
BspHI T'CATG_A
Cuts at: 2697 2697
Size: 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 571 785 1087 1468 571
Size: 214 302 381 2624
Fragments arranged by size:
2624 381 302 214
BsrBI CCG'CTC
Cuts at: 50 184 1813 1867 3351 3516 50
Size: 134 1629 54 1484 165 55
Fragments arranged by size:
1629 1484 165 134 55 54
BsrDI GCAATG_nn'
Cuts at: 1433 1433
Size: 3521
BsrFI r'CCGG_y
Cuts at: 142 1519 1700 1909 142
Size: 1377 181 209 1754
Fragments arranged by size:
1754 1377 209 181
BssSI C'ACGA_G
Cuts at: 1792 2150 1792
Size: 358 3163
BstAPI GCAn_nnn'nTGC
Cuts at: 898 898
Size: 3521
BstBI TT'CG_AA
Cuts at: 1882 1882
Size: 3521
BstYI r'GATC_y
Cuts at: 1 351 546 1013 1036 1371 1617 2629 2715
Size: 350 195 467 23 335 246 1012 86
Cuts at: 2715 2727 3073 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 350 346 335 246 195
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3521
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1471 1652 1815 1471
Size: 181 163 3177
Fragments arranged by size:
3177 181 163
BtsI GCAGTG_nn'
Cuts at: 764 764
Size: 3521
DraI TTT'AAA
Cuts at: 345 520 345
Size: 175 3346
DrdI GACnn_nn'nnGTC
Cuts at: 1227 2085 1227
Size: 858 2663
EaeI y'GGCC_r
Cuts at: 1106 1280 1671 1698 3043 1106
Size: 174 391 27 1345 1584
Fragments arranged by size:
1584 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1106 1106
Size: 3521
EarI CTCTTCn'nnn_
Cuts at: 1544 1754 1544
Size: 210 3311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1729 2039 2185 3331 1729
Size: 310 146 1146 1919
Fragments arranged by size:
1919 1146 310 146
EcoICRI GAG'CTC
Cuts at: 533 533
Size: 3521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1346 1778 2525 2793 227
Size: 1119 432 747 268 955
Fragments arranged by size:
1119 955 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 660 660
Size: 3521
EcoRI G'AATT_C
Cuts at: 525 525
Size: 3521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1540 1540
Size: 3521
FspI TGC'GCA
Cuts at: 1302 1302
Size: 3521
HaeII r_GCGC'y
Cuts at: 253 955 1203 2225 3204 253
Size: 702 248 1022 979 570
Fragments arranged by size:
1022 979 702 570 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1363 1393 1395 1425 1363
Size: 30 2 30 3459
Fragments arranged by size:
3459 30 30 2
HincII GTy'rAC
Cuts at: 560 560
Size: 3521
KasI G'GCGC_C
Cuts at: 951 1199 951
Size: 248 3273
KpnI G_GTAC'C
Cuts at: 541 541
Size: 3521
MluI A'CGCG_T
Cuts at: 2954 2954
Size: 3521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2376 2192
Size: 184 3337
MscI TGG'CCA
Cuts at: 1282 1282
Size: 3521
MslI CAynn'nnrTG
Cuts at: 1510 1637 1510
Size: 127 3394
MspA1I CmG'CkG
Cuts at: 632 946 1306 2319 2564 632
Size: 314 360 1013 245 1589
Fragments arranged by size:
1589 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1702 1702
Size: 3521
NarI GG'CG_CC
Cuts at: 952 1200 952
Size: 248 3273
NcoI C'CATG_G
Cuts at: 107 107
Size: 3521
NgoMIV G'CCGG_C
Cuts at: 1700 1700
Size: 3521
NheI G'CTAG_C
Cuts at: 486 486
Size: 3521
NspI r_CATG'y
Cuts at: 574 1074 1981 574
Size: 500 907 2114
Fragments arranged by size:
2114 907 500
PciI A'CATG_T
Cuts at: 1977 1977
Size: 3521
PflMI CCAn_nnn'nTGG
Cuts at: 318 318
Size: 3521
PmeI GTTT'AAAC
Cuts at: 520 520
Size: 3521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 3489
PstI C_TGCA'G
Cuts at: 568 1253 568
Size: 685 2836
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3521
PvuII CAG'CTG
Cuts at: 946 1306 946
Size: 360 3161
RsrII CG'GwC_CG
Cuts at: 1716 1716
Size: 3521
SacI G_AGCT'C
Cuts at: 535 535
Size: 3521
SalI G'TCGA_C
Cuts at: 558 558
Size: 3521
SapI GCTCTTCn'nnn_
Cuts at: 1544 1754 1544
Size: 210 3311
SbfI CC_TGCA'GG
Cuts at: 568 568
Size: 3521
ScaI AGT'ACT
Cuts at: 3038 3038
Size: 3521
SfcI C'TryA_G
Cuts at: 33 564 1249 2242 2433 33
Size: 531 685 993 191 1121
Fragments arranged by size:
1121 993 685 531 191
SfoI GGC'GCC
Cuts at: 953 1201 953
Size: 248 3273
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
SmaI CCC'GGG
Cuts at: 543 543
Size: 3521
SmlI C'TyrA_G
Cuts at: 676 1591 2083 2345 676
Size: 915 492 262 1852
Fragments arranged by size:
1852 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3521
SphI G_CATG'C
Cuts at: 574 1074 574
Size: 500 3021
StuI AGG'CCT
Cuts at: 237 403 237
Size: 166 3355
StyI C'CwwG_G
Cuts at: 107 655 1009 107
Size: 548 354 2619
Fragments arranged by size:
2619 548 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 171 1104 1237 1696 1905 171
Size: 933 133 459 209 1787
Fragments arranged by size:
1787 933 459 209 133
TatI w'GTAC_w
Cuts at: 3036 3036
Size: 3521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3489
Tth111I GACn'n_nGTC
Cuts at: 1318 1318
Size: 3521
XbaI T'CTAG_A
Cuts at: 552 552
Size: 3521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 165 1016 165
Size: 851 2670
XmaI C'CCGG_G
Cuts at: 541 541
Size: 3521
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI