Restriction map for
pF1KB0902
(Circular) (Six-base) MAPSORT of: pF1KB0902.seq Check: 564 from: 1 to: 4004
pF1KB0902 4004 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 661 661
Size: 4004
AccI GT'mk_AC
Cuts at: 458 548 1042 458
Size: 90 494 3420
Fragments arranged by size:
3420 494 90
Acc65I G'GTAC_C
Cuts at: 1020 1020
Size: 4004
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 940 1829 2261 3008 3276 133
Size: 807 889 432 747 268 861
Fragments arranged by size:
889 861 807 747 432 268
AflIII A'CryG_T
Cuts at: 679 1356 2460 3437 679
Size: 677 1104 977 1246
Fragments arranged by size:
1246 1104 977 677
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AhdI GACnn_n'nnGTC
Cuts at: 198 198
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 1309 2876 1309
Size: 1567 2437
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 1008 3357 3613 1008
Size: 2349 256 1399
Fragments arranged by size:
2349 1399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 1024 1024
Size: 4004
AvrII C'CTAG_G
Cuts at: 513 513
Size: 4004
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 1020 1434 1682 1717 1020
Size: 414 248 35 3307
Fragments arranged by size:
3307 414 248 35
BanII G_rGCy'C
Cuts at: 1018 2048 1018
Size: 1030 2974
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BciVI GTATCCnnnnn_n'
Cuts at: 614 1902 2663 4004 614
Size: 1288 761 1341 614
Fragments arranged by size:
1341 1288 761 614
BclI T'GATC_A
Cuts at: 774 1524 774
Size: 750 3254
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 218 745 1629 1722 2778 218
Size: 527 884 93 1056 1444
Fragments arranged by size:
1444 1056 884 527 93
BmgBI CAC'GTC
Cuts at: 682 682
Size: 4004
BmrI ACTGGGnnnn_n'
Cuts at: 654 1216 1297 1632 3776 654
Size: 562 81 335 2144 882
Fragments arranged by size:
2144 882 562 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 1180 2059 2551 2849 543
Size: 637 879 492 298 1698
Fragments arranged by size:
1698 879 637 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 519 3708 519
Size: 3189 815
BsaAI yAC'GTr
Cuts at: 1357 1357
Size: 4004
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 1435 1683 2385 3881 1435
Size: 248 702 1496 1558
Fragments arranged by size:
1558 1496 702 248
BsaWI w'CCGG_w
Cuts at: 764 1193 1714 2392 2666 2813 3224 764
Size: 429 521 678 274 147 411 1544
Fragments arranged by size:
1544 678 521 429 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 295 313 316 319 490 913 280
Size: 15 18 3 3 171 423 3371
Fragments arranged by size:
3371 423 171 18 15 3 3
BseYI C'CCAG_C
Cuts at: 130 242 1429 2187 2764 3260 130
Size: 112 1187 758 577 496 874
Fragments arranged by size:
1187 874 758 577 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 928
Size: 4004
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1018 1796 1986 2778 1018
Size: 778 190 792 2244
Fragments arranged by size:
2244 792 778 190
BsmI GAATG_Cn'
Cuts at: 381 974 381
Size: 593 3411
Bsp1286I G_dGCh'C
Cuts at: 218 745 1018 1629 1722 1796 1986 2048 2778
Size: 527 273 611 93 74 190 62 730
Cuts at: 2778 218
Size: 1444
Fragments arranged by size:
1444 730 611 527 273 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 3180 3180
Size: 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 1054 1268 1570 1951 661
Size: 393 214 302 381 2714
Fragments arranged by size:
2714 393 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 885 1381 885
Size: 496 3508
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 177 846 177
Size: 669 3335
BstYI r'GATC_y
Cuts at: 1 1029 1496 1519 1854 2100 3112 3198 3210
Size: 1028 467 23 335 246 1012 86 12
Cuts at: 3210 3556 1
Size: 346 449
Fragments arranged by size:
1028 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 329 107
Size: 222 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1954 2135 2298 1954
Size: 181 163 3660
Fragments arranged by size:
3660 181 163
BtsI GCAGTG_nn'
Cuts at: 1247 1247
Size: 4004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 926 961 926
Size: 35 3969
DraI TTT'AAA
Cuts at: 792 1003 792
Size: 211 3793
DraIII CAC_nnn'GTG
Cuts at: 885 885
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 710 1589 1763 2154 2181 3526 710
Size: 879 174 391 27 1345 1188
Fragments arranged by size:
1345 1188 879 391 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 380 485 590 820 2027 2237 380
Size: 105 105 230 1207 210 2147
Fragments arranged by size:
2147 1207 230 210 105 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1016 1016
Size: 4004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 940 1829 2261 3008 3276 133
Size: 807 889 432 747 268 861
Fragments arranged by size:
889 861 807 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 151 151
Size: 4004
EcoO109I rG'GnC_Cy
Cuts at: 192 1143 192
Size: 951 3053
EcoRI G'AATT_C
Cuts at: 1008 1008
Size: 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 4004
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 759 791 1846 1876 1878 1908 184
Size: 32 543 32 1055 30 2 30 2280
Fragments arranged by size:
2280 1055 543 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 1024 1024
Size: 4004
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 911 2675 2859 911
Size: 1764 184 2056
Fragments arranged by size:
2056 1764 184
MscI TGG'CCA
Cuts at: 712 1765 712
Size: 1053 2951
MslI CAynn'nnrTG
Cuts at: 844 1993 2120 844
Size: 1149 127 2728
Fragments arranged by size:
2728 1149 127
MspA1I CmG'CkG
Cuts at: 209 246 708 1115 1429 1789 2802 3047 209
Size: 37 462 407 314 360 1013 245 1166
Fragments arranged by size:
1166 1013 462 407 360 314 245 37
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NcoI C'CATG_G
Cuts at: 107 329 107
Size: 222 3782
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 786 1057 1557 2464 786
Size: 271 500 907 2326
Fragments arranged by size:
2326 907 500 271
PciI A'CATG_T
Cuts at: 2460 2460
Size: 4004
PfoI T'CCnGG_A
Cuts at: 862 862
Size: 4004
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 407 439 3143 3175 407
Size: 32 2704 32 1236
Fragments arranged by size:
2704 1236 32 32
PpuMI rG'GwC_Cy
Cuts at: 192 192
Size: 4004
PstI C_TGCA'G
Cuts at: 1051 1736 1051
Size: 685 3319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 209 246 708 1429 1789 209
Size: 37 462 721 360 2424
Fragments arranged by size:
2424 721 462 360 37
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 1018 1018
Size: 4004
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 590 2027 2237 590
Size: 1437 210 2357
Fragments arranged by size:
2357 1437 210
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 1047 1732 2725 2916 33
Size: 1014 685 993 191 1121
Fragments arranged by size:
1121 1014 993 685 191
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 1026 1026
Size: 4004
SmlI C'TyrA_G
Cuts at: 522 1159 2074 2566 2828 522
Size: 637 915 492 262 1698
Fragments arranged by size:
1698 915 637 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StuI AGG'CCT
Cuts at: 988 988
Size: 4004
StyI C'CwwG_G
Cuts at: 107 329 513 1138 1492 107
Size: 222 184 625 354 2619
Fragments arranged by size:
2619 625 354 222 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 3519 3519
Size: 4004
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 3521 3553 148
Size: 32 3341 32 599
Fragments arranged by size:
3341 599 32 32
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 705 1499 705
Size: 794 3210
XmaI C'CCGG_G
Cuts at: 1024 1024
Size: 4004
XmnI GAAnn'nnTTC
Cuts at: 814 974 814
Size: 160 3844
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI