(Circular) (Six-base) MAPSORT of: pF1KB0869.seq Check: 990 from: 1 to: 6335 pF1KB0869 6335 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1600 3147 1600 Size: 1547 4788 AatII G_ACGT'C Cuts at: 2482 2482 Size: 6335 AccI GT'mk_AC Cuts at: 1328 3373 1328 Size: 2045 4290 Acc65I G'GTAC_C Cuts at: 1407 3092 3351 1407 Size: 1685 259 4391 Fragments arranged by size: 4391 1685 259 AclI AA'CG_TT Cuts at: 6175 6175 Size: 6335 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 327 1579 2239 4160 4592 5339 5607 327 Size: 1252 660 1921 432 747 268 1055 Fragments arranged by size: 1921 1252 1055 747 660 432 268 AfeI AGC'GCT Cuts at: 620 1352 1966 620 Size: 732 614 4989 Fragments arranged by size: 4989 732 614 AflIII A'CryG_T Cuts at: 2066 3008 3687 4791 5768 2066 Size: 942 679 1104 977 2633 Fragments arranged by size: 2633 1104 977 942 679 AgeI A'CCGG_T Cuts at: 485 4723 485 Size: 4238 2097 AleI CACnn'nnGTG Cuts at: 1036 1736 1036 Size: 700 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1453 2629 1453 Size: 1176 5159 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 195 227 2649 2681 195 Size: 32 2422 32 3849 Fragments arranged by size: 3849 2422 32 32 AlwNI CAG_nnn'CTG Cuts at: 1197 1500 1592 2144 2393 3640 5207 1197 Size: 303 92 552 249 1247 1567 2325 Fragments arranged by size: 2325 1567 1247 552 303 249 92 ApaI G_GGCC'C Cuts at: 1109 1605 1861 1109 Size: 496 256 5583 Fragments arranged by size: 5583 496 256 ApaLI G'TGCA_C Cuts at: 5105 5105 Size: 6335 ApoI r'AATT_y Cuts at: 786 2328 3339 5688 5944 786 Size: 1542 1011 2349 256 1177 Fragments arranged by size: 2349 1542 1177 1011 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6335 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6335 AvaI C'yCGr_G Cuts at: 860 3355 860 Size: 2495 3840 AvrII C'CTAG_G Cuts at: 699 699 Size: 6335 BamHI G'GATC_C Cuts at: 1789 3360 1789 Size: 1571 4764 BanI G'GyrC_C Cuts at: 1180 1407 1565 2383 3092 3351 3765 4013 4048 Size: 227 158 818 709 259 414 248 35 Cuts at: 4048 1180 Size: 3467 Fragments arranged by size: 3467 818 709 414 259 248 227 158 35 BanII G_rGCy'C Cuts at: 644 1109 1457 1481 1605 1861 3349 4379 644 Size: 465 348 24 124 256 1488 1030 2600 Fragments arranged by size: 2600 1488 1030 465 348 256 124 24 BbeI G_GCGC'C Cuts at: 3769 4017 3769 Size: 248 6087 BbsI GAAGACnn'nnnn_ Cuts at: 908 1062 2230 2352 3286 908 Size: 154 1168 122 934 3957 Fragments arranged by size: 3957 1168 934 154 122 BbvCI CC'TCA_GC Cuts at: 1056 1056 Size: 6335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1854 1888 2285 2319 1854 Size: 34 397 34 5870 Fragments arranged by size: 5870 397 34 34 BciVI GTATCCnnnnn_n' Cuts at: 1160 4233 4994 6335 1160 Size: 3073 761 1341 1160 Fragments arranged by size: 3073 1341 1160 761 BclI T'GATC_A Cuts at: 564 3855 564 Size: 3291 3044 BfrBI ATG'CAT Cuts at: 511 1254 511 Size: 743 5592 BglI GCCn_nnn'nGGC Cuts at: 2317 3211 2317 Size: 894 5441 BglII A'GATC_T Cuts at: 3306 3850 5887 3306 Size: 544 2037 3754 Fragments arranged by size: 3754 2037 544 BlpI GC'TnA_GC Cuts at: 3034 3447 3034 Size: 413 5922 Bme1580I G_kGCm'C Cuts at: 1109 1286 1605 1861 3960 4053 5109 1109 Size: 177 319 256 2099 93 1056 2335 Fragments arranged by size: 2335 2099 1056 319 256 177 93 BmgBI CAC'GTC Cuts at: 676 1332 676 Size: 656 5679 BmrI ACTGGGnnnn_n' Cuts at: 2617 3284 3547 3628 3963 6107 2617 Size: 667 263 81 335 2144 2845 Fragments arranged by size: 2845 2144 667 335 263 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4702 4702 Size: 6335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 834 1675 834 Size: 841 5494 Bpu10I CC'TnA_GC Cuts at: 1001 1056 1078 1666 2213 1001 Size: 55 22 588 547 5123 Fragments arranged by size: 5123 588 547 55 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 126 376 1410 3511 4390 4882 5180 126 Size: 250 1034 2101 879 492 298 1281 Fragments arranged by size: 2101 1281 1034 879 492 298 250 BsaI GGTCTCn'nnnn_ Cuts at: 1751 1925 6039 1751 Size: 174 4114 2047 Fragments arranged by size: 4114 2047 174 BsaAI yAC'GTr Cuts at: 1203 1320 1542 3688 1203 Size: 117 222 2146 3850 Fragments arranged by size: 3850 2146 222 117 BsaBI GATnn'nnATC Cuts at: 6 3849 5886 6 Size: 3843 2037 455 Fragments arranged by size: 3843 2037 455 BsaHI Gr'CG_yC Cuts at: 1756 2479 2843 3766 4014 4716 6212 1756 Size: 723 364 923 248 702 1496 1879 Fragments arranged by size: 1879 1496 923 723 702 364 248 BsaWI w'CCGG_w Cuts at: 485 2525 3524 4045 4723 4997 5144 5555 485 Size: 2040 999 521 678 274 147 411 1265 Fragments arranged by size: 2040 1265 999 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 701 731 2173 2203 701 Size: 30 1442 30 4833 Fragments arranged by size: 4833 1442 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 613 704 1672 3049 613 Size: 91 968 1377 3899 Fragments arranged by size: 3899 1377 968 91 BseYI C'CCAG_C Cuts at: 602 1622 2081 2431 3760 4518 5095 5591 602 Size: 1020 459 350 1329 758 577 496 1346 Fragments arranged by size: 1346 1329 1020 758 577 496 459 350 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 510 1357 1486 2186 2514 2740 510 Size: 847 129 700 328 226 4105 Fragments arranged by size: 4105 847 700 328 226 129 BsiEI CG_ry'CG Cuts at: 104 486 2923 3877 3923 5131 104 Size: 382 2437 954 46 1208 1308 Fragments arranged by size: 2437 1308 1208 954 382 46 BsiHKAI G_wGCw'C Cuts at: 644 1457 1541 2899 3349 4127 4317 5109 644 Size: 813 84 1358 450 778 190 792 1870 Fragments arranged by size: 1870 1358 813 792 778 450 190 84 BsmI GAATG_Cn' Cuts at: 273 511 2123 2516 2573 2608 273 Size: 238 1612 393 57 35 4000 Fragments arranged by size: 4000 1612 393 238 57 35 BsmBI CGTCTCn'nnnn_ Cuts at: 3138 3138 Size: 6335 Bsp1286I G_dGCh'C Cuts at: 644 1109 1286 1457 1481 1541 1605 1861 2899 Size: 465 177 171 24 60 64 256 1038 Cuts at: 2899 3349 3960 4053 4127 4317 4379 5109 644 Size: 450 611 93 74 190 62 730 1870 Fragments arranged by size: 1870 1038 730 611 465 450 256 190 177 171 93 74 64 62 60 24 BspEI T'CCGG_A Cuts at: 5555 5555 Size: 6335 BspHI T'CATG_A Cuts at: 920 5511 920 Size: 4591 1744 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1600 3147 3385 3599 3901 4282 1600 Size: 1547 238 214 302 381 3653 Fragments arranged by size: 3653 1547 381 302 238 214 BsrBI CCG'CTC Cuts at: 50 536 1811 2705 4627 4681 6165 6330 50 Size: 486 1275 894 1922 54 1484 165 55 Fragments arranged by size: 1922 1484 1275 894 486 165 55 54 BsrDI GCAATG_nn' Cuts at: 1046 2046 2181 4247 1046 Size: 1000 135 2066 3134 Fragments arranged by size: 3134 2066 1000 135 BsrFI r'CCGG_y Cuts at: 485 2918 4333 4514 4723 485 Size: 2433 1415 181 209 2097 Fragments arranged by size: 2433 2097 1415 209 181 BssSI C'ACGA_G Cuts at: 1914 4606 4964 1914 Size: 2692 358 3285 Fragments arranged by size: 3285 2692 358 BstAPI GCAn_nnn'nTGC Cuts at: 1500 1592 2894 3712 1500 Size: 92 1302 818 4123 Fragments arranged by size: 4123 1302 818 92 BstBI TT'CG_AA Cuts at: 4696 4696 Size: 6335 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 6335 BstYI r'GATC_y Cuts at: 1 360 557 1496 1550 1789 2141 2968 3306 Size: 359 197 939 54 239 352 827 338 Cuts at: 3306 3360 3827 3850 4185 4431 5443 5529 5541 Size: 54 467 23 335 246 1012 86 12 Cuts at: 5541 5887 1 Size: 346 449 Fragments arranged by size: 1012 939 827 467 449 359 352 346 338 335 246 239 197 86 54 54 23 12 BtgI C'CryG_G Cuts at: 659 1184 1721 1750 1784 2882 2909 3026 659 Size: 525 537 29 34 1098 27 117 3968 Fragments arranged by size: 3968 1098 537 525 117 34 29 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1071 4285 4466 4629 1071 Size: 3214 181 163 2777 Fragments arranged by size: 3214 2777 181 163 BtsI GCAGTG_nn' Cuts at: 1684 1840 1855 3578 1684 Size: 156 15 1723 4441 Fragments arranged by size: 4441 1723 156 15 DraI TTT'AAA Cuts at: 3334 3334 Size: 6335 DrdI GACnn_nn'nnGTC Cuts at: 1917 2484 4041 4899 1917 Size: 567 1557 858 3353 Fragments arranged by size: 3353 1557 858 567 EaeI y'GGCC_r Cuts at: 1373 2274 2920 3920 4094 4485 4512 5857 1373 Size: 901 646 1000 174 391 27 1345 1851 Fragments arranged by size: 1851 1345 1000 901 646 391 174 27 EagI C'GGCC_G Cuts at: 2920 3920 2920 Size: 1000 5335 EarI CTCTTCn'nnn_ Cuts at: 346 1439 1462 1555 2759 2978 4358 4568 346 Size: 1093 23 93 1204 219 1380 210 2113 Fragments arranged by size: 2113 1380 1204 1093 219 210 93 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1485 4543 4853 4999 6145 1485 Size: 3058 310 146 1146 1675 Fragments arranged by size: 3058 1675 1146 310 146 EcoICRI GAG'CTC Cuts at: 642 1455 3347 642 Size: 813 1892 3630 Fragments arranged by size: 3630 1892 813 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 327 834 1579 1675 2239 4160 4592 5339 5607 Size: 507 745 96 564 1921 432 747 268 Cuts at: 5607 327 Size: 1055 Fragments arranged by size: 1921 1055 747 745 564 507 432 268 96 EcoO109I rG'GnC_Cy Cuts at: 1106 1194 1601 1608 1857 1858 2086 2699 3474 Size: 88 407 7 249 1 228 613 775 Cuts at: 3474 1106 Size: 3967 Fragments arranged by size: 3967 775 613 407 249 228 88 7 1 EcoRI G'AATT_C Cuts at: 3339 3339 Size: 6335 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4354 4354 Size: 6335 FspI TGC'GCA Cuts at: 4116 4116 Size: 6335 HaeII r_GCGC'y Cuts at: 622 891 1085 1354 1784 1968 3769 4017 5039 Size: 269 194 269 430 184 1801 248 1022 Cuts at: 5039 6018 622 Size: 979 939 Fragments arranged by size: 1801 1022 979 939 430 269 269 248 194 184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 349 381 549 581 1698 1730 1781 1813 2173 Size: 32 168 32 1117 32 51 32 360 Cuts at: 2173 2205 2339 2371 2726 2758 2788 2820 4177 Size: 32 134 32 355 32 30 32 1357 Cuts at: 4177 4207 4209 4239 349 Size: 30 2 30 2445 Fragments arranged by size: 2445 1357 1117 360 355 168 134 51 32 32 32 32 32 32 32 32 30 30 30 2 HincII GTy'rAC Cuts at: 290 1329 2256 2457 3374 290 Size: 1039 927 201 917 3251 Fragments arranged by size: 3251 1039 927 917 201 HpaI GTT'AAC Cuts at: 290 290 Size: 6335 KasI G'GCGC_C Cuts at: 3765 4013 3765 Size: 248 6087 KpnI G_GTAC'C Cuts at: 1411 3096 3355 1411 Size: 1685 259 4391 Fragments arranged by size: 4391 1685 259 MluI A'CGCG_T Cuts at: 5768 5768 Size: 6335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1729 2786 2795 5006 5190 1729 Size: 1057 9 2211 184 2874 Fragments arranged by size: 2874 2211 1057 184 9 MscI TGG'CCA Cuts at: 1375 4096 1375 Size: 2721 3614 MslI CAynn'nnrTG Cuts at: 463 830 919 1036 1249 1736 1772 4324 4451 Size: 367 89 117 213 487 36 2552 127 Cuts at: 4451 463 Size: 2347 Fragments arranged by size: 2552 2347 487 367 213 127 117 89 36 MspA1I CmG'CkG Cuts at: 587 631 1060 1433 3090 3446 3760 4120 5133 Size: 44 429 373 1657 356 314 360 1013 Cuts at: 5133 5378 587 Size: 245 1544 Fragments arranged by size: 1657 1544 1013 429 373 360 356 314 245 44 NaeI GCC'GGC Cuts at: 2920 4516 2920 Size: 1596 4739 NarI GG'CG_CC Cuts at: 3766 4014 3766 Size: 248 6087 NcoI C'CATG_G Cuts at: 659 2882 659 Size: 2223 4112 NgoMIV G'CCGG_C Cuts at: 2918 4514 2918 Size: 1596 4739 NsiI A_TGCA'T Cuts at: 513 1256 513 Size: 743 5592 NspI r_CATG'y Cuts at: 2070 2306 2678 3012 3388 3888 4795 2070 Size: 236 372 334 376 500 907 3610 Fragments arranged by size: 3610 907 500 376 372 334 236 PasI CC'CwG_GG Cuts at: 180 180 Size: 6335 PciI A'CATG_T Cuts at: 2066 3008 4791 2066 Size: 942 1783 3610 Fragments arranged by size: 3610 1783 942 PflMI CCAn_nnn'nTGG Cuts at: 381 2234 2629 381 Size: 1853 395 4087 Fragments arranged by size: 4087 1853 395 PfoI T'CCnGG_A Cuts at: 669 1885 2899 669 Size: 1216 1014 4105 Fragments arranged by size: 4105 1216 1014 PmeI GTTT'AAAC Cuts at: 3334 3334 Size: 6335 PmlI CAC'GTG Cuts at: 1320 1542 1320 Size: 222 6113 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5474 5506 5474 Size: 32 6303 PpuMI rG'GwC_Cy Cuts at: 1608 2086 2699 1608 Size: 478 613 5244 Fragments arranged by size: 5244 613 478 PshAI GACnn'nnGTC Cuts at: 2251 2251 Size: 6335 PspOMI G'GGCC_C Cuts at: 1105 1601 1857 1105 Size: 496 256 5583 Fragments arranged by size: 5583 496 256 PstI C_TGCA'G Cuts at: 1229 1496 1930 1975 2509 3382 4067 1229 Size: 267 434 45 534 873 685 3497 Fragments arranged by size: 3497 873 685 534 434 267 45 PvuI CG_AT'CG Cuts at: 104 104 Size: 6335 PvuII CAG'CTG Cuts at: 631 1060 1433 3760 4120 631 Size: 429 373 2327 360 2846 Fragments arranged by size: 2846 2327 429 373 360 RsrII CG'GwC_CG Cuts at: 4530 4530 Size: 6335 SacI G_AGCT'C Cuts at: 644 1457 3349 644 Size: 813 1892 3630 Fragments arranged by size: 3630 1892 813 SalI G'TCGA_C Cuts at: 1327 3372 1327 Size: 2045 4290 SapI GCTCTTCn'nnn_ Cuts at: 1462 4358 4568 1462 Size: 2896 210 3229 Fragments arranged by size: 3229 2896 210 SbfI CC_TGCA'GG Cuts at: 3382 3382 Size: 6335 ScaI AGT'ACT Cuts at: 987 5852 987 Size: 4865 1470 SfcI C'TryA_G Cuts at: 33 594 653 1225 1492 1926 1971 2505 3378 Size: 561 59 572 267 434 45 534 873 Cuts at: 3378 4063 5056 5247 33 Size: 685 993 191 1121 Fragments arranged by size: 1121 993 873 685 572 561 534 434 267 191 59 45 SfoI GGC'GCC Cuts at: 3767 4015 3767 Size: 248 6087 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6335 SmaI CCC'GGG Cuts at: 862 3357 862 Size: 2495 3840 SmlI C'TyrA_G Cuts at: 141 355 1425 3490 4405 4897 5159 141 Size: 214 1070 2065 915 492 262 1317 Fragments arranged by size: 2065 1317 1070 915 492 262 214 SpeI A'CTAG_T Cuts at: 67 67 Size: 6335 SphI G_CATG'C Cuts at: 3388 3888 3388 Size: 500 5835 StuI AGG'CCT Cuts at: 415 1096 415 Size: 681 5654 StyI C'CwwG_G Cuts at: 659 699 2089 2577 2882 3469 3823 659 Size: 40 1390 488 305 587 354 3171 Fragments arranged by size: 3171 1390 587 488 354 305 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 185 466 1228 3918 4051 4510 4719 185 Size: 281 762 2690 133 459 209 1801 Fragments arranged by size: 2690 1801 762 459 281 209 133 TatI w'GTAC_w Cuts at: 731 985 5850 731 Size: 254 4865 1216 Fragments arranged by size: 4865 1216 254 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 376 408 2620 2652 5852 5884 376 Size: 32 2212 32 3200 32 827 Fragments arranged by size: 3200 2212 827 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4132 4132 Size: 6335 XbaI T'CTAG_A Cuts at: 3366 3366 Size: 6335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1174 2069 2116 3830 1174 Size: 895 47 1714 3679 Fragments arranged by size: 3679 1714 895 47 XmaI C'CCGG_G Cuts at: 860 3355 860 Size: 2495 3840 ZraI GAC'GTC Cuts at: 2480 2480 Size: 6335 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AflII AhdI AscI BaeI BmtI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI NruI PacI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI