Restriction map for
pF1KB0668
(Circular) (Six-base) MAPSORT of: pF1KB0668.seq Check: 50 from: 1 to: 6113
pF1KB0668 6113 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3151 3151
Size: 6113
Acc65I G'GTAC_C
Cuts at: 840 3129 840
Size: 2289 3824
AclI AA'CG_TT
Cuts at: 5953 5953
Size: 6113
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 2006 2176 2992 3056 3938 4370 4723 5385
Size: 1209 170 816 64 882 432 353 662
Cuts at: 5385 797
Size: 1525
Fragments arranged by size:
1525 1209 882 816 662 432 353 170
64
AflIII A'CryG_T
Cuts at: 3465 5265 5546 3465
Size: 1800 281 4032
Fragments arranged by size:
4032 1800 281
AgeI A'CCGG_T
Cuts at: 801 801
Size: 6113
AhdI GACnn_n'nnGTC
Cuts at: 1884 1884
Size: 6113
AleI CACnn'nnGTG
Cuts at: 1642 1642
Size: 6113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 860 860
Size: 6113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 749 781 749
Size: 32 6081
AlwNI CAG_nnn'CTG
Cuts at: 3418 4856 3418
Size: 1438 4675
ApaLI G'TGCA_C
Cuts at: 4951 4951
Size: 6113
ApoI r'AATT_y
Cuts at: 564 629 1409 1905 3065 3117 5466 5722 564
Size: 65 780 496 1160 52 2349 256 955
Fragments arranged by size:
2349 1160 955 780 496 256 65 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 6113
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6113
AvaI C'yCGr_G
Cuts at: 3133 3133
Size: 6113
BamHI G'GATC_C
Cuts at: 481 3138 481
Size: 2657 3456
BanI G'GyrC_C
Cuts at: 142 840 1244 1342 2410 3129 3543 3791 3826
Size: 698 404 98 1068 719 414 248 35
Cuts at: 3826 142
Size: 2429
Fragments arranged by size:
2429 1068 719 698 414 404 248 98
35
BanII G_rGCy'C
Cuts at: 861 3127 4157 861
Size: 2266 1030 2817
Fragments arranged by size:
2817 2266 1030
BbeI G_GCGC'C
Cuts at: 146 2414 3547 3795 146
Size: 2268 1133 248 2464
Fragments arranged by size:
2464 2268 1133 248
BbsI GAAGACnn'nnnn_
Cuts at: 2305 2366 2780 2305
Size: 61 414 5638
Fragments arranged by size:
5638 414 61
BciVI GTATCCnnnnn_n'
Cuts at: 637 2162 4011 5067 6113 637
Size: 1525 1849 1056 1046 637
Fragments arranged by size:
1849 1525 1056 1046 637
BclI T'GATC_A
Cuts at: 3633 3633
Size: 6113
BfrBI ATG'CAT
Cuts at: 502 502
Size: 6113
BglII A'GATC_T
Cuts at: 3628 5665 3628
Size: 2037 4076
BlpI GC'TnA_GC
Cuts at: 3225 3225
Size: 6113
Bme1580I G_kGCm'C
Cuts at: 166 215 1345 1921 2295 3738 3831 4955 166
Size: 49 1130 576 374 1443 93 1124 1324
Fragments arranged by size:
1443 1324 1130 1124 576 374 93 49
BmrI ACTGGGnnnn_n'
Cuts at: 217 1113 2790 3325 3406 3741 5885 217
Size: 896 1677 535 81 335 2144 445
Fragments arranged by size:
2144 1677 896 535 445 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 2714 4480 2712
Size: 2 1766 4345
Fragments arranged by size:
4345 1766 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 770
Size: 6113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1415 3289 4168 4882 5180 1415
Size: 1874 879 714 298 2348
Fragments arranged by size:
2348 1874 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2483 5817 2483
Size: 3334 2779
BsaAI yAC'GTr
Cuts at: 3466 3466
Size: 6113
BsaBI GATnn'nnATC
Cuts at: 6 3627 5664 6
Size: 3621 2037 455
Fragments arranged by size:
3621 2037 455
BsaHI Gr'CG_yC
Cuts at: 143 1634 2411 3544 3792 4494 5990 143
Size: 1491 777 1133 248 702 1496 266
Fragments arranged by size:
1496 1491 1133 777 702 266 248
BsaWI w'CCGG_w
Cuts at: 801 1026 3302 3823 4501 4912 5059 5333 801
Size: 225 2276 521 678 411 147 274 1581
Fragments arranged by size:
2276 1581 678 521 411 274 225 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 171 174 307 1126 1201 1311 2522 171
Size: 3 133 819 75 110 1211 3762
Fragments arranged by size:
3762 1211 819 133 110 75 3
BseYI C'CCAG_C
Cuts at: 704 3101 3538 4296 4961 5369 704
Size: 2397 437 758 665 408 1448
Fragments arranged by size:
2397 1448 758 665 437 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 838
Size: 6113
BsiEI CG_ry'CG
Cuts at: 104 3655 3701 4931 104
Size: 3551 46 1230 1286
Fragments arranged by size:
3551 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 540 2781 3127 3905 4095 4955 540
Size: 2241 346 778 190 860 1698
Fragments arranged by size:
2241 1698 860 778 346 190
BsmI GAATG_Cn'
Cuts at: 504 504
Size: 6113
BsmBI CGTCTCn'nnnn_
Cuts at: 1122 1122
Size: 6113
Bsp1286I G_dGCh'C
Cuts at: 166 215 540 861 1345 1921 2295 2781 3127
Size: 49 325 321 484 576 374 486 346
Cuts at: 3127 3738 3831 3905 4095 4157 4955 166
Size: 611 93 74 190 62 798 1324
Fragments arranged by size:
1324 798 611 576 486 484 374 346
325 321 190 93 74 62 49
BspEI T'CCGG_A
Cuts at: 1026 1026
Size: 6113
BspHI T'CATG_A
Cuts at: 4545 4545
Size: 6113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 828 852 3163 3377 3679 4060 828
Size: 24 2311 214 302 381 2881
Fragments arranged by size:
2881 2311 381 302 214 24
BsrBI CCG'CTC
Cuts at: 50 4405 4459 5943 6108 50
Size: 4355 54 1484 165 55
Fragments arranged by size:
4355 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 692 4025 692
Size: 3333 2780
BsrFI r'CCGG_y
Cuts at: 801 825 4111 4292 801
Size: 24 3286 181 2622
Fragments arranged by size:
3286 2622 181 24
BsrGI T'GTAC_A
Cuts at: 2140 2140
Size: 6113
BssSI C'ACGA_G
Cuts at: 4384 5092 4384
Size: 708 5405
BstAPI GCAn_nnn'nTGC
Cuts at: 3490 3490
Size: 6113
BstBI TT'CG_AA
Cuts at: 520 4474 520
Size: 3954 2159
BstXI CCAn_nnnn'nTGG
Cuts at: 667 1337 1610 1644 667
Size: 670 273 34 5136
Fragments arranged by size:
5136 670 273 34
BstYI r'GATC_y
Cuts at: 1 481 790 1023 2707 3138 3605 3628 3963
Size: 480 309 233 1684 431 467 23 335
Cuts at: 3963 4209 4515 4527 4613 5665 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1684 1052 480 467 449 431 335 309
306 246 233 86 23 12
BtgI C'CryG_G
Cuts at: 107 191 107
Size: 84 6029
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4063 4244 4407 4063
Size: 181 163 5769
Fragments arranged by size:
5769 181 163
BtsI GCAGTG_nn'
Cuts at: 381 721 2261 3356 381
Size: 340 1540 1095 3138
Fragments arranged by size:
3138 1540 1095 340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 267 302 267
Size: 35 6078
DraI TTT'AAA
Cuts at: 3112 3112
Size: 6113
DrdI GACnn_nn'nnGTC
Cuts at: 3819 5163 3819
Size: 1344 4769
EaeI y'GGCC_r
Cuts at: 110 188 2261 2822 3698 3872 4263 4290 5635
Size: 78 2073 561 876 174 391 27 1345
Cuts at: 5635 110
Size: 588
Fragments arranged by size:
2073 1345 876 588 561 391 174 78
27
EagI C'GGCC_G
Cuts at: 3698 3698
Size: 6113
EarI CTCTTCn'nnn_
Cuts at: 1103 1982 3011 4136 4346 1103
Size: 879 1029 1125 210 2870
Fragments arranged by size:
2870 1125 1029 879 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4321 5063 5209 5923 4321
Size: 742 146 714 4511
Fragments arranged by size:
4511 742 714 146
EcoICRI GAG'CTC
Cuts at: 3125 3125
Size: 6113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 770 797 2006 2176 2992 3056 3938 4370 4723
Size: 27 1209 170 816 64 882 432 353
Cuts at: 4723 5385 770
Size: 662 1498
Fragments arranged by size:
1498 1209 882 816 662 432 353 170
64 27
EcoO109I rG'GnC_Cy
Cuts at: 730 3252 730
Size: 2522 3591
EcoRI G'AATT_C
Cuts at: 629 3117 629
Size: 2488 3625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4132 4132
Size: 6113
FspI TGC'GCA
Cuts at: 3894 3894
Size: 6113
HaeII r_GCGC'y
Cuts at: 146 1062 2414 3547 3795 5025 5796 146
Size: 916 1352 1133 248 1230 771 463
Fragments arranged by size:
1352 1230 1133 916 771 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1773 1805 1870 1902 2110 2142 3955 3985 3987
Size: 32 65 32 208 32 1813 30 2
Cuts at: 3987 4017 1773
Size: 30 3869
Fragments arranged by size:
3869 1813 208 65 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1437 3152 1437
Size: 1715 4398
KasI G'GCGC_C
Cuts at: 142 2410 3543 3791 142
Size: 2268 1133 248 2464
Fragments arranged by size:
2464 2268 1133 248
KpnI G_GTAC'C
Cuts at: 844 3133 844
Size: 2289 3824
MfeI C'AATT_G
Cuts at: 1277 1757 2483 1277
Size: 480 726 4907
Fragments arranged by size:
4907 726 480
MluI A'CGCG_T
Cuts at: 5546 5546
Size: 6113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 411 1792 2904 4872 5056 411
Size: 1381 1112 1968 184 1468
Fragments arranged by size:
1968 1468 1381 1112 184
MscI TGG'CCA
Cuts at: 2824 3874 2824
Size: 1050 5063
MslI CAynn'nnrTG
Cuts at: 1642 1793 4102 4229 1642
Size: 151 2309 127 3526
Fragments arranged by size:
3526 2309 151 127
MspA1I CmG'CkG
Cuts at: 137 708 3224 3538 3898 4682 4927 137
Size: 571 2516 314 360 784 245 1323
Fragments arranged by size:
2516 1323 784 571 360 314 245
NaeI GCC'GGC
Cuts at: 4294 4294
Size: 6113
NarI GG'CG_CC
Cuts at: 143 2411 3544 3792 143
Size: 2268 1133 248 2464
Fragments arranged by size:
2464 2268 1133 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6113
NgoMIV G'CCGG_C
Cuts at: 4292 4292
Size: 6113
NsiI A_TGCA'T
Cuts at: 504 504
Size: 6113
NspI r_CATG'y
Cuts at: 1798 3109 3166 3666 5269 1798
Size: 1311 57 500 1603 2642
Fragments arranged by size:
2642 1603 1311 500 57
PasI CC'CwG_GG
Cuts at: 1370 1370
Size: 6113
PciI A'CATG_T
Cuts at: 5265 5265
Size: 6113
PflMI CCAn_nnn'nTGG
Cuts at: 754 1681 754
Size: 927 5186
PfoI T'CCnGG_A
Cuts at: 786 786
Size: 6113
PmeI GTTT'AAAC
Cuts at: 3112 3112
Size: 6113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4559 4591 4559
Size: 32 6081
PsiI TTA'TAA
Cuts at: 1733 1733
Size: 6113
PstI C_TGCA'G
Cuts at: 420 713 3160 3845 420
Size: 293 2447 685 2688
Fragments arranged by size:
2688 2447 685 293
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6113
PvuII CAG'CTG
Cuts at: 708 3538 3898 708
Size: 2830 360 2923
Fragments arranged by size:
2923 2830 360
RsrII CG'GwC_CG
Cuts at: 4308 4308
Size: 6113
SacI G_AGCT'C
Cuts at: 3127 3127
Size: 6113
SalI G'TCGA_C
Cuts at: 3150 3150
Size: 6113
SapI GCTCTTCn'nnn_
Cuts at: 1982 3011 4136 4346 1982
Size: 1029 1125 210 3749
Fragments arranged by size:
3749 1125 1029 210
SbfI CC_TGCA'GG
Cuts at: 3160 3160
Size: 6113
ScaI AGT'ACT
Cuts at: 5630 5630
Size: 6113
SexAI A'CCwGG_T
Cuts at: 531 531
Size: 6113
SfcI C'TryA_G
Cuts at: 33 416 709 3156 3841 4809 5000 33
Size: 383 293 2447 685 968 191 1146
Fragments arranged by size:
2447 1146 968 685 383 293 191
SfoI GGC'GCC
Cuts at: 144 2412 3545 3793 144
Size: 2268 1133 248 2464
Fragments arranged by size:
2464 2268 1133 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6113
SmaI CCC'GGG
Cuts at: 3135 3135
Size: 6113
SmlI C'TyrA_G
Cuts at: 1430 3268 4183 4897 5159 1430
Size: 1838 915 714 262 2384
Fragments arranged by size:
2384 1838 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6113
SphI G_CATG'C
Cuts at: 1798 3166 3666 1798
Size: 1368 500 4245
Fragments arranged by size:
4245 1368 500
SspI AAT'ATT
Cuts at: 393 1656 1871 2089 2565 393
Size: 1263 215 218 476 3941
Fragments arranged by size:
3941 1263 476 218 215
StuI AGG'CCT
Cuts at: 235 235
Size: 6113
StyI C'CwwG_G
Cuts at: 107 306 624 2175 2471 2897 3247 3601 107
Size: 199 318 1551 296 426 350 354 2619
Fragments arranged by size:
2619 1551 426 354 350 318 296 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 244 1097 1615 3696 3829 4288 4497 244
Size: 853 518 2081 133 459 209 1860
Fragments arranged by size:
2081 1860 853 518 459 209 133
TatI w'GTAC_w
Cuts at: 1135 2140 5628 1135
Size: 1005 3488 1620
Fragments arranged by size:
3488 1620 1005
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5630 5662 5630
Size: 32 6081
Tth111I GACn'n_nGTC
Cuts at: 3910 3910
Size: 6113
XbaI T'CTAG_A
Cuts at: 3144 3144
Size: 6113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3608 3608
Size: 6113
XmaI C'CCGG_G
Cuts at: 3133 3133
Size: 6113
XmnI GAAnn'nnTTC
Cuts at: 564 564
Size: 6113
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI BsiWI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
PacI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI