Restriction map for
pF1KB0471
(Circular) (Six-base) MAPSORT of: pF1KB0471.seq Check: 4587 from: 1 to: 4658
pF1KB0471 4658 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 316 1696 316
Size: 1380 3278
Acc65I G'GTAC_C
Cuts at: 1674 1674
Size: 4658
AclI AA'CG_TT
Cuts at: 460 4498 460
Size: 4038 620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 1547 2483 2915 3662 3930 120
Size: 1427 936 432 747 268 848
Fragments arranged by size:
1427 936 848 747 432 268
AflII C'TTAA_G
Cuts at: 921 921
Size: 4658
AflIII A'CryG_T
Cuts at: 500 2010 3114 4091 500
Size: 1510 1104 977 1067
Fragments arranged by size:
1510 1104 1067 977
AgeI A'CCGG_T
Cuts at: 3046 3046
Size: 4658
AhdI GACnn_n'nnGTC
Cuts at: 1135 1194 1135
Size: 59 4599
AleI CACnn'nnGTG
Cuts at: 889 889
Size: 4658
AlwNI CAG_nnn'CTG
Cuts at: 829 1963 3530 829
Size: 1134 1567 1957
Fragments arranged by size:
1957 1567 1134
ApaI G_GGCC'C
Cuts at: 1289 1289
Size: 4658
ApaLI G'TGCA_C
Cuts at: 3428 3428
Size: 4658
ApoI r'AATT_y
Cuts at: 246 565 681 948 1662 4011 4267 246
Size: 319 116 267 714 2349 256 637
Fragments arranged by size:
2349 714 637 319 267 256 116
AseI AT'TA_AT
Cuts at: 20 551 20
Size: 531 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
AvaI C'yCGr_G
Cuts at: 1678 1678
Size: 4658
BamHI G'GATC_C
Cuts at: 1488 1683 1488
Size: 195 4463
BanI G'GyrC_C
Cuts at: 1674 2088 2336 2371 1674
Size: 414 248 35 3961
Fragments arranged by size:
3961 414 248 35
BanII G_rGCy'C
Cuts at: 1238 1289 1672 2702 1238
Size: 51 383 1030 3194
Fragments arranged by size:
3194 1030 383 51
BbeI G_GCGC'C
Cuts at: 2092 2340 2092
Size: 248 4410
BbvCI CC'TCA_GC
Cuts at: 1262 1262
Size: 4658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4624
BciVI GTATCCnnnnn_n'
Cuts at: 2556 3317 4658 2556
Size: 761 1341 2556
Fragments arranged by size:
2556 1341 761
BclI T'GATC_A
Cuts at: 1596 2178 1596
Size: 582 4076
BfrBI ATG'CAT
Cuts at: 1125 1125
Size: 4658
BglII A'GATC_T
Cuts at: 2173 4210 2173
Size: 2037 2621
BlpI GC'TnA_GC
Cuts at: 1770 1770
Size: 4658
Bme1580I G_kGCm'C
Cuts at: 1289 2283 2376 3432 1289
Size: 994 93 1056 2515
Fragments arranged by size:
2515 1056 994 93
BmrI ACTGGGnnnn_n'
Cuts at: 1173 1870 1951 2286 4430 1173
Size: 697 81 335 2144 1401
Fragments arranged by size:
2144 1401 697 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 4658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1297
Size: 4658
Bpu10I CC'TnA_GC
Cuts at: 1262 1262
Size: 4658
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1299 1834 2713 3205 3503 1299
Size: 535 879 492 298 2454
Fragments arranged by size:
2454 879 535 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4362 4362
Size: 4658
BsaAI yAC'GTr
Cuts at: 2011 2011
Size: 4658
BsaBI GATnn'nnATC
Cuts at: 6 2172 4209 6
Size: 2166 2037 455
Fragments arranged by size:
2166 2037 455
BsaHI Gr'CG_yC
Cuts at: 2089 2337 3039 4535 2089
Size: 248 702 1496 2212
Fragments arranged by size:
2212 1496 702 248
BsaWI w'CCGG_w
Cuts at: 251 1847 2368 3046 3320 3467 3878 251
Size: 1596 521 678 274 147 411 1031
Fragments arranged by size:
1596 1031 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1290 1320 1327 1357 1290
Size: 30 7 30 4591
Fragments arranged by size:
4591 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1216 1219 1311 1216
Size: 3 92 4563
Fragments arranged by size:
4563 92 3
BseYI C'CCAG_C
Cuts at: 1552 2083 2841 3418 3914 1552
Size: 531 758 577 496 2296
Fragments arranged by size:
2296 758 577 531 496
BsiEI CG_ry'CG
Cuts at: 104 2200 2246 3454 104
Size: 2096 46 1208 1308
Fragments arranged by size:
2096 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 749 1238 1672 2450 2640 3432 749
Size: 489 434 778 190 792 1975
Fragments arranged by size:
1975 792 778 489 434 190
BsmI GAATG_Cn'
Cuts at: 129 129
Size: 4658
BsmBI CGTCTCn'nnnn_
Cuts at: 1463 1463
Size: 4658
Bsp1286I G_dGCh'C
Cuts at: 749 1238 1289 1672 2283 2376 2450 2640 2702
Size: 489 51 383 611 93 74 190 62
Cuts at: 2702 3432 749
Size: 730 1975
Fragments arranged by size:
1975 730 611 489 383 190 93 74
62 51
BspEI T'CCGG_A
Cuts at: 3878 3878
Size: 4658
BspHI T'CATG_A
Cuts at: 323 3834 323
Size: 3511 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 992 1223 1708 1922 2224 2605 992
Size: 231 485 214 302 381 3045
Fragments arranged by size:
3045 485 381 302 231 214
BsrBI CCG'CTC
Cuts at: 50 2950 3004 4488 4653 50
Size: 2900 54 1484 165 55
Fragments arranged by size:
2900 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 238 307 2570 238
Size: 69 2263 2326
Fragments arranged by size:
2326 2263 69
BsrFI r'CCGG_y
Cuts at: 2656 2837 3046 2656
Size: 181 209 4268
Fragments arranged by size:
4268 209 181
BssSI C'ACGA_G
Cuts at: 175 415 2929 3287 175
Size: 240 2514 358 1546
Fragments arranged by size:
2514 1546 358 240
BstAPI GCAn_nnn'nTGC
Cuts at: 829 2035 829
Size: 1206 3452
BstBI TT'CG_AA
Cuts at: 3019 3019
Size: 4658
BstXI CCAn_nnnn'nTGG
Cuts at: 1008 1008
Size: 4658
BstYI r'GATC_y
Cuts at: 1 1488 1683 2150 2173 2508 2754 3766 3852
Size: 1487 195 467 23 335 246 1012 86
Cuts at: 3852 3864 4210 1
Size: 12 346 449
Fragments arranged by size:
1487 1012 467 449 346 335 246 195
86 23 12
BstZ17I GTA'TAC
Cuts at: 317 317
Size: 4658
Bsu36I CC'TnA_GG
Cuts at: 1220 1220
Size: 4658
BtgI C'CryG_G
Cuts at: 1001 1346 1001
Size: 345 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2608 2789 2952 2608
Size: 181 163 4314
Fragments arranged by size:
4314 181 163
BtsI GCAGTG_nn'
Cuts at: 1901 1901
Size: 4658
DraI TTT'AAA
Cuts at: 1657 1657
Size: 4658
DrdI GACnn_nn'nnGTC
Cuts at: 2364 3222 2364
Size: 858 3800
EaeI y'GGCC_r
Cuts at: 491 804 970 1520 2243 2417 2808 2835 4180
Size: 313 166 550 723 174 391 27 1345
Cuts at: 4180 491
Size: 969
Fragments arranged by size:
1345 969 723 550 391 313 174 166
27
EagI C'GGCC_G
Cuts at: 2243 2243
Size: 4658
EarI CTCTTCn'nnn_
Cuts at: 1325 1463 1566 2681 2891 1325
Size: 138 103 1115 210 3092
Fragments arranged by size:
3092 1115 210 138 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2866 3176 3322 4468 2866
Size: 310 146 1146 3056
Fragments arranged by size:
3056 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1236 1670 1236
Size: 434 4224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 1297 1547 2483 2915 3662 3930 120
Size: 1177 250 936 432 747 268 848
Fragments arranged by size:
1177 936 848 747 432 268 250
EcoNI CCTnn'n_nnAGG
Cuts at: 1219 1293 1219
Size: 74 4584
EcoO109I rG'GnC_Cy
Cuts at: 1286 1317 1569 1797 1286
Size: 31 252 228 4147
Fragments arranged by size:
4147 252 228 31
EcoRI G'AATT_C
Cuts at: 1662 1662
Size: 4658
EcoRV GAT'ATC
Cuts at: 236 236
Size: 4658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 795 1625 2677 795
Size: 830 1052 2776
Fragments arranged by size:
2776 1052 830
FspI TGC'GCA
Cuts at: 2439 2439
Size: 4658
HaeII r_GCGC'y
Cuts at: 1096 2092 2340 3362 4341 1096
Size: 996 248 1022 979 1413
Fragments arranged by size:
1413 1022 996 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1121 1153 1180 1212 1290 1322 1327 1359 1375
Size: 32 27 32 78 32 5 32 16
Cuts at: 1375 1407 1576 1608 2500 2530 2532 2562 1121
Size: 32 169 32 892 30 2 30 3217
Fragments arranged by size:
3217 892 169 78 32 32 32 32
32 32 30 30 27 16 5 2
HincII GTy'rAC
Cuts at: 1479 1626 1697 1479
Size: 147 71 4440
Fragments arranged by size:
4440 147 71
KasI G'GCGC_C
Cuts at: 2088 2336 2088
Size: 248 4410
KpnI G_GTAC'C
Cuts at: 1678 1678
Size: 4658
MluI A'CGCG_T
Cuts at: 4091 4091
Size: 4658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 974 1250 3329 3513 974
Size: 276 2079 184 2119
Fragments arranged by size:
2119 2079 276 184
MscI TGG'CCA
Cuts at: 493 806 972 1522 2419 493
Size: 313 166 550 897 2732
Fragments arranged by size:
2732 897 550 313 166
MslI CAynn'nnrTG
Cuts at: 499 551 553 752 889 900 1006 2647 2774
Size: 52 2 199 137 11 106 1641 127
Cuts at: 2774 499
Size: 2383
Fragments arranged by size:
2383 1641 199 137 127 106 52 11
2
MspA1I CmG'CkG
Cuts at: 449 1526 1769 2083 2443 3456 3701 449
Size: 1077 243 314 360 1013 245 1406
Fragments arranged by size:
1406 1077 1013 360 314 245 243
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 4658
NarI GG'CG_CC
Cuts at: 2089 2337 2089
Size: 248 4410
NcoI C'CATG_G
Cuts at: 1001 1001
Size: 4658
NdeI CA'TA_TG
Cuts at: 192 192
Size: 4658
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 4658
NsiI A_TGCA'T
Cuts at: 1127 1127
Size: 4658
NspI r_CATG'y
Cuts at: 504 1711 2211 3118 504
Size: 1207 500 907 2044
Fragments arranged by size:
2044 1207 907 500
PciI A'CATG_T
Cuts at: 500 3114 500
Size: 2614 2044
PflMI CCAn_nnn'nTGG
Cuts at: 1518 1518
Size: 4658
PfoI T'CCnGG_A
Cuts at: 1313 1313
Size: 4658
PmeI GTTT'AAAC
Cuts at: 1657 1657
Size: 4658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4626
PpuMI rG'GwC_Cy
Cuts at: 1317 1317
Size: 4658
PsiI TTA'TAA
Cuts at: 1055 1055
Size: 4658
PspOMI G'GGCC_C
Cuts at: 1285 1285
Size: 4658
PstI C_TGCA'G
Cuts at: 1705 2390 1705
Size: 685 3973
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4658
PvuII CAG'CTG
Cuts at: 2083 2443 2083
Size: 360 4298
RsrII CG'GwC_CG
Cuts at: 2853 2853
Size: 4658
SacI G_AGCT'C
Cuts at: 1238 1672 1238
Size: 434 4224
SalI G'TCGA_C
Cuts at: 1695 1695
Size: 4658
SapI GCTCTTCn'nnn_
Cuts at: 2681 2891 2681
Size: 210 4448
SbfI CC_TGCA'GG
Cuts at: 1705 1705
Size: 4658
ScaI AGT'ACT
Cuts at: 4175 4175
Size: 4658
SfcI C'TryA_G
Cuts at: 33 1169 1338 1701 2386 3379 3570 33
Size: 1136 169 363 685 993 191 1121
Fragments arranged by size:
1136 1121 993 685 363 191 169
SfoI GGC'GCC
Cuts at: 2090 2338 2090
Size: 248 4410
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
SmaI CCC'GGG
Cuts at: 1680 1680
Size: 4658
SmlI C'TyrA_G
Cuts at: 921 1278 1813 2728 3220 3482 921
Size: 357 535 915 492 262 2097
Fragments arranged by size:
2097 915 535 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4658
SphI G_CATG'C
Cuts at: 1711 2211 1711
Size: 500 4158
StuI AGG'CCT
Cuts at: 421 1143 1156 421
Size: 722 13 3923
Fragments arranged by size:
3923 722 13
StyI C'CwwG_G
Cuts at: 1001 1629 1792 2146 1001
Size: 628 163 354 3513
Fragments arranged by size:
3513 628 354 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2241 2374 2833 3042 2241
Size: 133 459 209 3857
Fragments arranged by size:
3857 459 209 133
TatI w'GTAC_w
Cuts at: 4173 4173
Size: 4658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 780 812 4175 4207 780
Size: 32 3363 32 1231
Fragments arranged by size:
3363 1231 32 32
Tth111I GACn'n_nGTC
Cuts at: 2455 2455
Size: 4658
XbaI T'CTAG_A
Cuts at: 1689 1689
Size: 4658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1008 2153 1008
Size: 1145 3513
XmaI C'CCGG_G
Cuts at: 1678 1678
Size: 4658
XmnI GAAnn'nnTTC
Cuts at: 127 127
Size: 4658
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI AscI AvrII BaeI
BbsI BglI BmgBI BmtI BsgI BsiWI BsrGI BssHII
BstEII ClaI CspCI DraIII FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PmlI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI