Restriction map for
pF1KB0470
(Circular) (Six-base) MAPSORT of: pF1KB0470.seq Check: 6904 from: 1 to: 6086
pF1KB0470 6086 bp
With 182 enzymes: SgfI *
September 24, 2008 10:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 1704 387
Size: 1317 4769
AccI GT'mk_AC
Cuts at: 2336 3124 2336
Size: 788 5298
Acc65I G'GTAC_C
Cuts at: 3102 3102
Size: 6086
AclI AA'CG_TT
Cuts at: 5926 5926
Size: 6086
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1463 1831 3050 3911 4343 5090 5358 721
Size: 742 368 1219 861 432 747 268 1449
Fragments arranged by size:
1449 1219 861 747 742 432 368 268
AfeI AGC'GCT
Cuts at: 346 346
Size: 6086
AflIII A'CryG_T
Cuts at: 3438 4542 5519 3438
Size: 1104 977 4005
Fragments arranged by size:
4005 1104 977
AgeI A'CCGG_T
Cuts at: 4474 4474
Size: 6086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 640 864 640
Size: 224 5862
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 525 557 525
Size: 32 6054
AlwNI CAG_nnn'CTG
Cuts at: 191 306 688 921 1590 1720 2703 2925 2968
Size: 115 382 233 669 130 983 222 43
Cuts at: 2968 3391 4958 191
Size: 423 1567 1319
Fragments arranged by size:
1567 1319 983 669 423 382 233 222
130 115 43
ApaI G_GGCC'C
Cuts at: 1051 1211 1342 1051
Size: 160 131 5795
Fragments arranged by size:
5795 160 131
ApaLI G'TGCA_C
Cuts at: 2347 2893 4856 2347
Size: 546 1963 3577
Fragments arranged by size:
3577 1963 546
ApoI r'AATT_y
Cuts at: 3090 5439 5695 3090
Size: 2349 256 3481
Fragments arranged by size:
3481 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6086
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6086
AvaI C'yCGr_G
Cuts at: 144 388 1052 1111 1198 1607 1670 1733 2439
Size: 244 664 59 87 409 63 63 706
Cuts at: 2439 2582 3106 144
Size: 143 524 3124
Fragments arranged by size:
3124 706 664 524 409 244 143 87
63 63 59
BamHI G'GATC_C
Cuts at: 2535 2817 3111 2535
Size: 282 294 5510
Fragments arranged by size:
5510 294 282
BanI G'GyrC_C
Cuts at: 148 1123 1655 1844 1993 2362 2949 3102 3516
Size: 975 532 189 149 369 587 153 414
Cuts at: 3516 3764 3799 148
Size: 248 35 2435
Fragments arranged by size:
2435 975 587 532 414 369 248 189
153 149 35
BanII G_rGCy'C
Cuts at: 145 263 275 359 581 695 898 1051 1075
Size: 118 12 84 222 114 203 153 24
Cuts at: 1075 1112 1211 1304 1342 1677 1904 2045 3100
Size: 37 99 93 38 335 227 141 1055
Cuts at: 3100 4130 145
Size: 1030 2101
Fragments arranged by size:
2101 1055 1030 335 227 222 203 153
141 118 114 99 93 84 38 37
24 12
BbeI G_GCGC'C
Cuts at: 3520 3768 3520
Size: 248 5838
BbsI GAAGACnn'nnnn_
Cuts at: 1451 1673 1451
Size: 222 5864
BbvCI CC'TCA_GC
Cuts at: 212 2633 212
Size: 2421 3665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6052
BciVI GTATCCnnnnn_n'
Cuts at: 2680 3984 4745 6086 2680
Size: 1304 761 1341 2680
Fragments arranged by size:
2680 1341 1304 761
BclI T'GATC_A
Cuts at: 1385 3606 1385
Size: 2221 3865
BglI GCCn_nnn'nGGC
Cuts at: 170 363 1729 2143 2782 2791 170
Size: 193 1366 414 639 9 3465
Fragments arranged by size:
3465 1366 639 414 193 9
BglII A'GATC_T
Cuts at: 3601 5638 3601
Size: 2037 4049
BlpI GC'TnA_GC
Cuts at: 613 2005 3198 613
Size: 1392 1193 3501
Fragments arranged by size:
3501 1392 1193
Bme1580I G_kGCm'C
Cuts at: 153 1051 1126 1199 1211 1342 1658 2351 2897
Size: 898 75 73 12 131 316 693 546
Cuts at: 2897 3711 3804 4860 153
Size: 814 93 1056 1379
Fragments arranged by size:
1379 1056 898 814 693 546 316 131
93 75 73 12
BmgBI CAC'GTC
Cuts at: 1377 2346 1377
Size: 969 5117
BmrI ACTGGGnnnn_n'
Cuts at: 247 641 1224 1307 2349 2384 2662 2916 3298
Size: 394 583 83 1042 35 278 254 382
Cuts at: 3298 3379 3714 5858 247
Size: 81 335 2144 475
Fragments arranged by size:
2144 1042 583 475 394 382 335 278
254 83 81 35
BmtI G_CTAG'C
Cuts at: 493 493
Size: 6086
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1272 1307 4453 1272
Size: 35 3146 2905
Fragments arranged by size:
3146 2905 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 604 751 1378 1471 1516 1534 1728 2531
Size: 9 147 627 93 45 18 194 803
Cuts at: 2531 2579 2896 595
Size: 48 317 3785
Fragments arranged by size:
3785 803 627 317 194 147 93 48
45 18 9
Bpu10I CC'TnA_GC
Cuts at: 212 2633 212
Size: 2421 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3262 4141 4633 4931 3262
Size: 879 492 298 4417
Fragments arranged by size:
4417 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 793 1747 5790 793
Size: 954 4043 1089
Fragments arranged by size:
4043 1089 954
BsaAI yAC'GTr
Cuts at: 1980 3439 1980
Size: 1459 4627
BsaBI GATnn'nnATC
Cuts at: 6 2575 3600 5637 6
Size: 2569 1025 2037 455
Fragments arranged by size:
2569 2037 1025 455
BsaHI Gr'CG_yC
Cuts at: 2774 3004 3517 3765 4467 5963 2774
Size: 230 513 248 702 1496 2897
Fragments arranged by size:
2897 1496 702 513 248 230
BsaWI w'CCGG_w
Cuts at: 1904 3275 3796 4474 4748 4895 5306 1904
Size: 1371 521 678 274 147 411 2684
Fragments arranged by size:
2684 1371 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1807 1837 1899 1929 2711 2741 1807
Size: 30 62 30 782 30 5152
Fragments arranged by size:
5152 782 62 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 804 1144 1535 1975 3073 804
Size: 340 391 440 1098 3817
Fragments arranged by size:
3817 1098 440 391 340
BseYI C'CCAG_C
Cuts at: 299 637 775 1117 1509 1594 1686 2141 2366
Size: 338 138 342 392 85 92 455 225
Cuts at: 2366 3511 4269 4846 5342 299
Size: 1145 758 577 496 1043
Fragments arranged by size:
1145 1043 758 577 496 455 392 342
338 225 138 92 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 292 215
Size: 77 6009
BsiEI CG_ry'CG
Cuts at: 104 1863 3628 3674 4882 104
Size: 1759 1765 46 1208 1308
Fragments arranged by size:
1765 1759 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 665 875 1502 1825 1856 1985 2351 2399 2897
Size: 210 627 323 31 129 366 48 498
Cuts at: 2897 3100 3878 4068 4860 665
Size: 203 778 190 792 1891
Fragments arranged by size:
1891 792 778 627 498 366 323 210
203 190 129 48 31
BsmI GAATG_Cn'
Cuts at: 557 557
Size: 6086
BsmBI CGTCTCn'nnnn_
Cuts at: 580 580
Size: 6086
Bsp1286I G_dGCh'C
Cuts at: 145 153 263 275 359 581 665 695 875
Size: 8 110 12 84 222 84 30 180
Cuts at: 875 898 1051 1075 1112 1126 1199 1211 1304
Size: 23 153 24 37 14 73 12 93
Cuts at: 1304 1342 1502 1658 1677 1825 1856 1904 1985
Size: 38 160 156 19 148 31 48 81
Cuts at: 1985 2045 2351 2399 2897 3100 3711 3804 3878
Size: 60 306 48 498 203 611 93 74
Cuts at: 3878 4068 4130 4860 145
Size: 190 62 730 1371
Fragments arranged by size:
1371 730 611 498 306 222 203 190
180 160 156 153 148 110 93 93
84 84 81 74 73 62 60 48
48 38 37 31 30 24 23 19
14 12 12 8
BspEI T'CCGG_A
Cuts at: 1904 5306 1904
Size: 3402 2684
BspHI T'CATG_A
Cuts at: 5262 5262
Size: 6086
BspMI ACCTGCnnnn'nnnn_
Cuts at: 239 387 1704 3136 3350 3652 4033 239
Size: 148 1317 1432 214 302 381 2292
Fragments arranged by size:
2292 1432 1317 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 605 735 1251 1324 1543 1896 1974 2004
Size: 555 130 516 73 219 353 78 30
Cuts at: 2004 2870 4378 4432 5916 6081 50
Size: 866 1508 54 1484 165 55
Fragments arranged by size:
1508 1484 866 555 516 353 219 165
130 78 73 55 54 30
BsrDI GCAATG_nn'
Cuts at: 277 3998 277
Size: 3721 2365
BsrFI r'CCGG_y
Cuts at: 1658 1858 2291 4084 4265 4474 1658
Size: 200 433 1793 181 209 3270
Fragments arranged by size:
3270 1793 433 209 200 181
BssSI C'ACGA_G
Cuts at: 2186 4357 4715 2186
Size: 2171 358 3557
Fragments arranged by size:
3557 2171 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3463 3463
Size: 6086
BstBI TT'CG_AA
Cuts at: 4447 4447
Size: 6086
BstEII G'GTnAC_C
Cuts at: 2638 2638
Size: 6086
BstXI CCAn_nnnn'nTGG
Cuts at: 282 474 2945 282
Size: 192 2471 3423
Fragments arranged by size:
3423 2471 192
BstYI r'GATC_y
Cuts at: 1 2324 2535 2817 3111 3578 3601 3936 4182
Size: 2323 211 282 294 467 23 335 246
Cuts at: 4182 5194 5280 5292 5638 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2323 1012 467 449 346 335 294 282
246 211 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1167 1526 1562 1167
Size: 359 36 5691
Fragments arranged by size:
5691 359 36
BtgI C'CryG_G
Cuts at: 224 371 1098 1515 2252 2352 2528 224
Size: 147 727 417 737 100 176 3782
Fragments arranged by size:
3782 737 727 417 176 147 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2268 2398 2562 4036 4217 4380 2268
Size: 130 164 1474 181 163 3974
Fragments arranged by size:
3974 1474 181 164 163 130
BtsI GCAGTG_nn'
Cuts at: 748 2160 2732 2996 3329 748
Size: 1412 572 264 333 3505
Fragments arranged by size:
3505 1412 572 333 264
DraI TTT'AAA
Cuts at: 3085 3085
Size: 6086
DraIII CAC_nnn'GTG
Cuts at: 2656 2656
Size: 6086
DrdI GACnn_nn'nnGTC
Cuts at: 3792 4650 3792
Size: 858 5228
EaeI y'GGCC_r
Cuts at: 1276 1860 2279 2748 3671 3845 4236 4263 5608
Size: 584 419 469 923 174 391 27 1345
Cuts at: 5608 1276
Size: 1754
Fragments arranged by size:
1754 1345 923 584 469 419 391 174
27
EagI C'GGCC_G
Cuts at: 1860 3671 1860
Size: 1811 4275
EarI CTCTTCn'nnn_
Cuts at: 325 1093 1943 2804 4109 4319 325
Size: 768 850 861 1305 210 2092
Fragments arranged by size:
2092 1305 861 850 768 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4294 4604 4750 5896 4294
Size: 310 146 1146 4484
Fragments arranged by size:
4484 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3098 3098
Size: 6086
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 604 721 751 1378 1463 1471 1516 1534
Size: 9 117 30 627 85 8 45 18
Cuts at: 1534 1728 1831 2531 2579 2896 3050 3911 4343
Size: 194 103 700 48 317 154 861 432
Cuts at: 4343 5090 5358 595
Size: 747 268 1323
Fragments arranged by size:
1323 861 747 700 627 432 317 268
194 154 117 103 85 48 45 30
18 9 8
EcoO109I rG'GnC_Cy
Cuts at: 131 188 208 403 1031 1048 1208 1339 1537
Size: 57 20 195 628 17 160 131 198
Cuts at: 1537 1766 3038 3225 131
Size: 229 1272 187 2992
Fragments arranged by size:
2992 1272 628 229 198 195 187 160
131 57 20 17
EcoRI G'AATT_C
Cuts at: 3090 3090
Size: 6086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4105 4105
Size: 6086
FspI TGC'GCA
Cuts at: 676 3867 676
Size: 3191 2895
HaeII r_GCGC'y
Cuts at: 125 306 348 907 2925 3520 3768 4790 5769
Size: 181 42 559 2018 595 248 1022 979
Cuts at: 5769 125
Size: 442
Fragments arranged by size:
2018 1022 979 595 559 442 248 181
42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 729 761 1942 1974 3025 3057 3928 3958 3960
Size: 32 1181 32 1051 32 871 30 2
Cuts at: 3960 3990 729
Size: 30 2825
Fragments arranged by size:
2825 1181 1051 871 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3125 3125
Size: 6086
KasI G'GCGC_C
Cuts at: 3516 3764 3516
Size: 248 5838
KpnI G_GTAC'C
Cuts at: 3106 3106
Size: 6086
MluI A'CGCG_T
Cuts at: 5519 5519
Size: 6086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1063 4757 4941 1063
Size: 3694 184 2208
Fragments arranged by size:
3694 2208 184
MscI TGG'CCA
Cuts at: 1278 2281 2750 3847 1278
Size: 1003 469 1097 3517
Fragments arranged by size:
3517 1097 1003 469
MslI CAynn'nnrTG
Cuts at: 382 846 2417 4075 4202 382
Size: 464 1571 1658 127 2266
Fragments arranged by size:
2266 1658 1571 464 127
MspA1I CmG'CkG
Cuts at: 246 918 1185 2055 2141 2149 2298 2477 3051
Size: 672 267 870 86 8 149 179 574
Cuts at: 3051 3197 3511 3871 4884 5129 246
Size: 146 314 360 1013 245 1203
Fragments arranged by size:
1203 1013 870 672 574 360 314 267
245 179 149 146 86 8
NaeI GCC'GGC
Cuts at: 1860 2293 4267 1860
Size: 433 1974 3679
Fragments arranged by size:
3679 1974 433
NarI GG'CG_CC
Cuts at: 3517 3765 3517
Size: 248 5838
NcoI C'CATG_G
Cuts at: 224 371 1098 1515 2252 2352 2528 224
Size: 147 727 417 737 100 176 3782
Fragments arranged by size:
3782 737 727 417 176 147 100
NgoMIV G'CCGG_C
Cuts at: 1858 2291 4265 1858
Size: 433 1974 3679
Fragments arranged by size:
3679 1974 433
NheI G'CTAG_C
Cuts at: 489 489
Size: 6086
NspI r_CATG'y
Cuts at: 2919 3139 3639 4546 2919
Size: 220 500 907 4459
Fragments arranged by size:
4459 907 500 220
PasI CC'CwG_GG
Cuts at: 1647 1647
Size: 6086
PciI A'CATG_T
Cuts at: 4542 4542
Size: 6086
PflMI CCAn_nnn'nTGG
Cuts at: 1104 1482 2656 1104
Size: 378 1174 4534
Fragments arranged by size:
4534 1174 378
PfoI T'CCnGG_A
Cuts at: 1157 1157
Size: 6086
PmeI GTTT'AAAC
Cuts at: 3085 3085
Size: 6086
PmlI CAC'GTG
Cuts at: 1980 1980
Size: 6086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5225 5257 5225
Size: 32 6054
PpuMI rG'GwC_Cy
Cuts at: 131 403 1031 1766 131
Size: 272 628 735 4451
Fragments arranged by size:
4451 735 628 272
PspOMI G'GGCC_C
Cuts at: 1047 1207 1338 1047
Size: 160 131 5795
Fragments arranged by size:
5795 160 131
PstI C_TGCA'G
Cuts at: 163 293 338 932 1145 1892 1958 3133 3818
Size: 130 45 594 213 747 66 1175 685
Cuts at: 3818 163
Size: 2431
Fragments arranged by size:
2431 1175 747 685 594 213 130 66
45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6086
PvuII CAG'CTG
Cuts at: 2298 3051 3511 3871 2298
Size: 753 460 360 4513
Fragments arranged by size:
4513 753 460 360
RsrII CG'GwC_CG
Cuts at: 4281 4281
Size: 6086
SacI G_AGCT'C
Cuts at: 3100 3100
Size: 6086
SalI G'TCGA_C
Cuts at: 3123 3123
Size: 6086
SanDI GG'GwC_CC
Cuts at: 131 403 131
Size: 272 5814
SapI GCTCTTCn'nnn_
Cuts at: 1943 2804 4109 4319 1943
Size: 861 1305 210 3710
Fragments arranged by size:
3710 1305 861 210
SbfI CC_TGCA'GG
Cuts at: 3133 3133
Size: 6086
ScaI AGT'ACT
Cuts at: 5603 5603
Size: 6086
SfcI C'TryA_G
Cuts at: 33 159 289 334 461 564 928 1141 1888
Size: 126 130 45 127 103 364 213 747
Cuts at: 1888 1954 3129 3814 4807 4998 33
Size: 66 1175 685 993 191 1121
Fragments arranged by size:
1175 1121 993 747 685 364 213 191
130 127 126 103 66 45
SfoI GGC'GCC
Cuts at: 3518 3766 3518
Size: 248 5838
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6086
SmaI CCC'GGG
Cuts at: 146 1200 1609 1672 2441 3108 146
Size: 1054 409 63 769 667 3124
Fragments arranged by size:
3124 1054 769 667 409 63
SmlI C'TyrA_G
Cuts at: 3241 4156 4648 4910 3241
Size: 915 492 262 4417
Fragments arranged by size:
4417 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6086
SphI G_CATG'C
Cuts at: 2919 3139 3639 2919
Size: 220 500 5366
Fragments arranged by size:
5366 500 220
SrfI GCCC'GGGC
Cuts at: 1200 1200
Size: 6086
StuI AGG'CCT
Cuts at: 332 1268 1939 2785 332
Size: 936 671 846 3633
Fragments arranged by size:
3633 936 846 671
StyI C'CwwG_G
Cuts at: 224 371 1098 1515 2252 2352 2528 2738 3220
Size: 147 727 417 737 100 176 210 482
Cuts at: 3220 3574 224
Size: 354 2736
Fragments arranged by size:
2736 737 727 482 417 354 210 176
147 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1791 1950 2380 2644 2937 3669 3802 4261 4470
Size: 159 430 264 293 732 133 459 209
Cuts at: 4470 1791
Size: 3407
Fragments arranged by size:
3407 732 459 430 293 264 209 159
133
TatI w'GTAC_w
Cuts at: 5601 5601
Size: 6086
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1807 1839 1899 1931 2711 2743 5603 5635 1807
Size: 32 60 32 780 32 2860 32 2258
Fragments arranged by size:
2860 2258 780 60 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 966 1801 2602 3883 966
Size: 835 801 1281 3169
Fragments arranged by size:
3169 1281 835 801
XbaI T'CTAG_A
Cuts at: 3117 3117
Size: 6086
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 726 888 1155 2871 3581 726
Size: 162 267 1716 710 3231
Fragments arranged by size:
3231 1716 710 267 162
XmaI C'CCGG_G
Cuts at: 144 1198 1607 1670 2439 3106 144
Size: 1054 409 63 769 667 3124
Fragments arranged by size:
3124 1054 769 667 409 63
XmnI GAAnn'nnTTC
Cuts at: 2203 2203
Size: 6086
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AscI AvrII BaeI BfrBI
BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI ZraI