Restriction map for
pF1KB0469
(Circular) (Six-base) MAPSORT of: pF1KB0469.seq Check: 9240 from: 1 to: 4658
pF1KB0469 4658 bp
With 182 enzymes: SgfI *
October 7, 2009 14:14 ..
AccI GT'mk_AC
Cuts at: 1696 1696
Size: 4658
Acc65I G'GTAC_C
Cuts at: 528 1674 528
Size: 1146 3512
AclI AA'CG_TT
Cuts at: 4498 4498
Size: 4658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 1429 1459 2483 2915 3662 3930 347
Size: 1082 30 1024 432 747 268 1075
Fragments arranged by size:
1082 1075 1024 747 432 268 30
AflIII A'CryG_T
Cuts at: 2010 3114 4091 2010
Size: 1104 977 2577
Fragments arranged by size:
2577 1104 977
AgeI A'CCGG_T
Cuts at: 3046 3046
Size: 4658
AlwNI CAG_nnn'CTG
Cuts at: 1963 3530 1963
Size: 1567 3091
ApaI G_GGCC'C
Cuts at: 196 232 196
Size: 36 4622
ApaLI G'TGCA_C
Cuts at: 3428 3428
Size: 4658
ApoI r'AATT_y
Cuts at: 500 656 1320 1662 4011 4267 500
Size: 156 664 342 2349 256 891
Fragments arranged by size:
2349 891 664 342 256 156
AseI AT'TA_AT
Cuts at: 20 1226 20
Size: 1206 3452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
AvaI C'yCGr_G
Cuts at: 116 195 1678 116
Size: 79 1483 3096
Fragments arranged by size:
3096 1483 79
BamHI G'GATC_C
Cuts at: 1683 1683
Size: 4658
BanI G'GyrC_C
Cuts at: 151 528 1674 2088 2336 2371 151
Size: 377 1146 414 248 35 2438
Fragments arranged by size:
2438 1146 414 377 248 35
BanII G_rGCy'C
Cuts at: 196 232 1569 1672 2702 196
Size: 36 1337 103 1030 2152
Fragments arranged by size:
2152 1337 1030 103 36
BbeI G_GCGC'C
Cuts at: 155 2092 2340 155
Size: 1937 248 2473
Fragments arranged by size:
2473 1937 248
BbsI GAAGACnn'nnnn_
Cuts at: 1315 1417 1315
Size: 102 4556
BbvCI CC'TCA_GC
Cuts at: 422 1181 422
Size: 759 3899
BciVI GTATCCnnnnn_n'
Cuts at: 2556 3317 4658 2556
Size: 761 1341 2556
Fragments arranged by size:
2556 1341 761
BclI T'GATC_A
Cuts at: 1049 1254 2178 1049
Size: 205 924 3529
Fragments arranged by size:
3529 924 205
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4658
BglII A'GATC_T
Cuts at: 1142 2173 4210 1142
Size: 1031 2037 1590
Fragments arranged by size:
2037 1590 1031
BlpI GC'TnA_GC
Cuts at: 1432 1770 1432
Size: 338 4320
Bme1580I G_kGCm'C
Cuts at: 196 232 2283 2376 3432 196
Size: 36 2051 93 1056 1422
Fragments arranged by size:
2051 1422 1056 93 36
BmgBI CAC'GTC
Cuts at: 939 939
Size: 4658
BmrI ACTGGGnnnn_n'
Cuts at: 378 981 1476 1870 1951 2286 4430 378
Size: 603 495 394 81 335 2144 606
Fragments arranged by size:
2144 606 603 495 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 4658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1075 775
Size: 300 4358
Bpu10I CC'TnA_GC
Cuts at: 422 1181 1568 422
Size: 759 387 3512
Fragments arranged by size:
3512 759 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1480 1834 2713 3205 3503 1480
Size: 354 879 492 298 2635
Fragments arranged by size:
2635 879 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 279 405 898 1439 4362 279
Size: 126 493 541 2923 575
Fragments arranged by size:
2923 575 541 493 126
BsaAI yAC'GTr
Cuts at: 2011 2011
Size: 4658
BsaBI GATnn'nnATC
Cuts at: 6 2172 4209 6
Size: 2166 2037 455
Fragments arranged by size:
2166 2037 455
BsaHI Gr'CG_yC
Cuts at: 152 2089 2337 3039 4535 152
Size: 1937 248 702 1496 275
Fragments arranged by size:
1937 1496 702 275 248
BsaWI w'CCGG_w
Cuts at: 1643 1847 2368 3046 3320 3467 3878 1643
Size: 204 521 678 274 147 411 2423
Fragments arranged by size:
2423 678 521 411 274 204 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1073 1103 1564 1579 1594 1609 1073
Size: 30 461 15 15 15 4122
Fragments arranged by size:
4122 461 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1543 1546 1543
Size: 3 4655
BseYI C'CCAG_C
Cuts at: 2083 2841 3418 3914 2083
Size: 758 577 496 2827
Fragments arranged by size:
2827 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1506 1624 1506
Size: 118 4540
BsiEI CG_ry'CG
Cuts at: 104 931 2200 2246 3454 104
Size: 827 1269 46 1208 1308
Fragments arranged by size:
1308 1269 1208 827 46
BsiHKAI G_wGCw'C
Cuts at: 1672 2450 2640 3432 1672
Size: 778 190 792 2898
Fragments arranged by size:
2898 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 929 1605 929
Size: 676 3982
Bsp1286I G_dGCh'C
Cuts at: 196 232 1569 1672 2283 2376 2450 2640 2702
Size: 36 1337 103 611 93 74 190 62
Cuts at: 2702 3432 196
Size: 730 1422
Fragments arranged by size:
1422 1337 730 611 190 103 93 74
62 36
BspEI T'CCGG_A
Cuts at: 1643 3878 1643
Size: 2235 2423
BspHI T'CATG_A
Cuts at: 590 3834 590
Size: 3244 1414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 426 1142 1708 1922 2224 2605 423
Size: 3 716 566 214 302 381 2476
Fragments arranged by size:
2476 716 566 381 302 214 3
BsrBI CCG'CTC
Cuts at: 50 257 274 1107 2950 3004 4488 4653 50
Size: 207 17 833 1843 54 1484 165 55
Fragments arranged by size:
1843 1484 833 207 165 55 54 17
BsrDI GCAATG_nn'
Cuts at: 2570 2570
Size: 4658
BsrFI r'CCGG_y
Cuts at: 2656 2837 3046 2656
Size: 181 209 4268
Fragments arranged by size:
4268 209 181
BssSI C'ACGA_G
Cuts at: 1102 2929 3287 1102
Size: 1827 358 2473
Fragments arranged by size:
2473 1827 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2035 2035
Size: 4658
BstBI TT'CG_AA
Cuts at: 3019 3019
Size: 4658
BstEII G'GTnAC_C
Cuts at: 1519 1519
Size: 4658
BstYI r'GATC_y
Cuts at: 1 428 552 1142 1175 1376 1683 2150 2173
Size: 427 124 590 33 201 307 467 23
Cuts at: 2173 2508 2754 3766 3852 3864 4210 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 590 467 449 427 346 335 307
246 201 124 86 33 23 12
Bsu36I CC'TnA_GG
Cuts at: 162 320 1451 162
Size: 158 1131 3369
Fragments arranged by size:
3369 1131 158
BtgI C'CryG_G
Cuts at: 107 332 107
Size: 225 4433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2608 2789 2952 2608
Size: 181 163 4314
Fragments arranged by size:
4314 181 163
BtsI GCAGTG_nn'
Cuts at: 1337 1901 1337
Size: 564 4094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1045 1080 1045
Size: 35 4623
DraI TTT'AAA
Cuts at: 993 1657 993
Size: 664 3994
DrdI GACnn_nn'nnGTC
Cuts at: 2364 3222 2364
Size: 858 3800
EaeI y'GGCC_r
Cuts at: 928 2243 2417 2808 2835 4180 928
Size: 1315 174 391 27 1345 1406
Fragments arranged by size:
1406 1345 1315 391 174 27
EagI C'GGCC_G
Cuts at: 928 2243 928
Size: 1315 3343
EarI CTCTTCn'nnn_
Cuts at: 813 2681 2891 813
Size: 1868 210 2580
Fragments arranged by size:
2580 1868 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 215 2866 3176 3322 4468 215
Size: 2651 310 146 1146 405
Fragments arranged by size:
2651 1146 405 310 146
EcoICRI GAG'CTC
Cuts at: 1670 1670
Size: 4658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 775 1075 1429 1459 2483 2915 3662 3930
Size: 428 300 354 30 1024 432 747 268
Cuts at: 3930 347
Size: 1075
Fragments arranged by size:
1075 1024 747 432 428 354 300 268
30
EcoO109I rG'GnC_Cy
Cuts at: 211 325 337 1095 1491 1797 211
Size: 114 12 758 396 306 3072
Fragments arranged by size:
3072 758 396 306 114 12
EcoRI G'AATT_C
Cuts at: 1320 1662 1320
Size: 342 4316
EcoRV GAT'ATC
Cuts at: 609 609
Size: 4658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4658
FspI TGC'GCA
Cuts at: 2439 2439
Size: 4658
HaeII r_GCGC'y
Cuts at: 155 1539 2092 2340 3362 4341 155
Size: 1384 553 248 1022 979 472
Fragments arranged by size:
1384 1022 979 553 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1347 1379 2500 2530 2532 2562 1347
Size: 32 1121 30 2 30 3443
Fragments arranged by size:
3443 1121 32 30 30 2
HincII GTy'rAC
Cuts at: 1245 1697 1245
Size: 452 4206
KasI G'GCGC_C
Cuts at: 151 2088 2336 151
Size: 1937 248 2473
Fragments arranged by size:
2473 1937 248
KpnI G_GTAC'C
Cuts at: 532 1678 532
Size: 1146 3512
MluI A'CGCG_T
Cuts at: 4091 4091
Size: 4658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 393 3329 3513 393
Size: 2936 184 1538
Fragments arranged by size:
2936 1538 184
MscI TGG'CCA
Cuts at: 2419 2419
Size: 4658
MslI CAynn'nnrTG
Cuts at: 2647 2774 2647
Size: 127 4531
MspA1I CmG'CkG
Cuts at: 1185 1407 1419 1769 2083 2443 3456 3701 1185
Size: 222 12 350 314 360 1013 245 2142
Fragments arranged by size:
2142 1013 360 350 314 245 222 12
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 4658
NarI GG'CG_CC
Cuts at: 152 2089 2337 152
Size: 1937 248 2473
Fragments arranged by size:
2473 1937 248
NcoI C'CATG_G
Cuts at: 107 332 107
Size: 225 4433
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 4658
NspI r_CATG'y
Cuts at: 1711 2211 3118 1711
Size: 500 907 3251
Fragments arranged by size:
3251 907 500
PasI CC'CwG_GG
Cuts at: 1562 1635 1562
Size: 73 4585
PciI A'CATG_T
Cuts at: 3114 3114
Size: 4658
PflMI CCAn_nnn'nTGG
Cuts at: 135 135
Size: 4658
PfoI T'CCnGG_A
Cuts at: 752 1091 752
Size: 339 4319
PmeI GTTT'AAAC
Cuts at: 1657 1657
Size: 4658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4626
PpuMI rG'GwC_Cy
Cuts at: 325 337 1095 1491 325
Size: 12 758 396 3492
Fragments arranged by size:
3492 758 396 12
PspOMI G'GGCC_C
Cuts at: 192 228 192
Size: 36 4622
PstI C_TGCA'G
Cuts at: 1139 1705 2390 1139
Size: 566 685 3407
Fragments arranged by size:
3407 685 566
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4658
PvuII CAG'CTG
Cuts at: 1407 2083 2443 1407
Size: 676 360 3622
Fragments arranged by size:
3622 676 360
RsrII CG'GwC_CG
Cuts at: 2853 2853
Size: 4658
SacI G_AGCT'C
Cuts at: 1672 1672
Size: 4658
SalI G'TCGA_C
Cuts at: 1695 1695
Size: 4658
SanDI GG'GwC_CC
Cuts at: 337 337
Size: 4658
SapI GCTCTTCn'nnn_
Cuts at: 2681 2891 2681
Size: 210 4448
SbfI CC_TGCA'GG
Cuts at: 1705 1705
Size: 4658
ScaI AGT'ACT
Cuts at: 4175 4175
Size: 4658
SexAI A'CCwGG_T
Cuts at: 288 288
Size: 4658
SfcI C'TryA_G
Cuts at: 33 1135 1701 2386 3379 3570 33
Size: 1102 566 685 993 191 1121
Fragments arranged by size:
1121 1102 993 685 566 191
SfoI GGC'GCC
Cuts at: 153 2090 2338 153
Size: 1937 248 2473
Fragments arranged by size:
2473 1937 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
SmaI CCC'GGG
Cuts at: 118 197 1680 118
Size: 79 1483 3096
Fragments arranged by size:
3096 1483 79
SmlI C'TyrA_G
Cuts at: 1495 1813 2728 3220 3482 1495
Size: 318 915 492 262 2671
Fragments arranged by size:
2671 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4658
SphI G_CATG'C
Cuts at: 1711 2211 1711
Size: 500 4158
SrfI GCCC'GGGC
Cuts at: 118 197 118
Size: 79 4579
StuI AGG'CCT
Cuts at: 544 619 544
Size: 75 4583
StyI C'CwwG_G
Cuts at: 107 188 332 824 1598 1792 2146 107
Size: 81 144 492 774 194 354 2619
Fragments arranged by size:
2619 774 492 354 194 144 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 922 2241 2374 2833 3042 922
Size: 1319 133 459 209 2538
Fragments arranged by size:
2538 1319 459 209 133
TatI w'GTAC_w
Cuts at: 952 4173 952
Size: 3221 1437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 663 695 1073 1105 1564 1596 4175 4207 663
Size: 32 378 32 459 32 2579 32 1114
Fragments arranged by size:
2579 1114 459 378 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2455 2455
Size: 4658
XbaI T'CTAG_A
Cuts at: 1365 1689 1365
Size: 324 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 907 2153 907
Size: 1246 3412
XmaI C'CCGG_G
Cuts at: 116 195 1678 116
Size: 79 1483 3096
Fragments arranged by size:
3096 1483 79
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstXI BstZ17I ClaI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI