Restriction map for
pF1KB0438
(Circular) (Six-base) MAPSORT of: pF1KB0438.seq Check: 6110 from: 1 to: 5720
pF1KB0438 5720 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1098 1157 2460 1098
Size: 59 1303 4358
Fragments arranged by size:
4358 1303 59
AatII G_ACGT'C
Cuts at: 173 173
Size: 5720
AccI GT'mk_AC
Cuts at: 797 2758 797
Size: 1961 3759
Acc65I G'GTAC_C
Cuts at: 377 2736 377
Size: 2359 3361
AclI AA'CG_TT
Cuts at: 5560 5560
Size: 5720
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2047 3545 3977 4724 4992 1561
Size: 486 1498 432 747 268 2289
Fragments arranged by size:
2289 1498 747 486 432 268
AflIII A'CryG_T
Cuts at: 1416 3072 4176 5153 1416
Size: 1656 1104 977 1983
Fragments arranged by size:
1983 1656 1104 977
AgeI A'CCGG_T
Cuts at: 380 4108 380
Size: 3728 1992
AhdI GACnn_n'nnGTC
Cuts at: 1805 1805
Size: 5720
AleI CACnn'nnGTG
Cuts at: 661 1327 661
Size: 666 5054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2128 2128
Size: 5720
AlwNI CAG_nnn'CTG
Cuts at: 1437 1620 1656 2025 2097 2142 2181 3025 4592
Size: 183 36 369 72 45 39 844 1567
Cuts at: 4592 1437
Size: 2565
Fragments arranged by size:
2565 1567 844 369 183 72 45 39
36
ApaI G_GGCC'C
Cuts at: 646 1354 646
Size: 708 5012
ApaLI G'TGCA_C
Cuts at: 4490 4490
Size: 5720
ApoI r'AATT_y
Cuts at: 492 2724 5073 5329 492
Size: 2232 2349 256 883
Fragments arranged by size:
2349 2232 883 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5720
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5720
AvaI C'yCGr_G
Cuts at: 353 398 637 1153 1179 1298 2168 2233 2335
Size: 45 239 516 26 119 870 65 102
Cuts at: 2335 2740 353
Size: 405 3333
Fragments arranged by size:
3333 870 516 405 239 119 102 65
45 26
BamHI G'GATC_C
Cuts at: 2406 2745 2406
Size: 339 5381
BanI G'GyrC_C
Cuts at: 377 811 1530 1683 2736 3150 3398 3433 377
Size: 434 719 153 1053 414 248 35 2664
Fragments arranged by size:
2664 1053 719 434 414 248 153 35
BanII G_rGCy'C
Cuts at: 646 1204 1354 2203 2336 2734 3764 646
Size: 558 150 849 133 398 1030 2602
Fragments arranged by size:
2602 1030 849 558 398 150 133
BbeI G_GCGC'C
Cuts at: 815 3154 3402 815
Size: 2339 248 3133
Fragments arranged by size:
3133 2339 248
BbsI GAAGACnn'nnnn_
Cuts at: 538 1881 538
Size: 1343 4377
BbvCI CC'TCA_GC
Cuts at: 607 1020 1138 1836 607
Size: 413 118 698 4491
Fragments arranged by size:
4491 698 413 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1379 1413 1379
Size: 34 5686
BciVI GTATCCnnnnn_n'
Cuts at: 3618 4379 5720 3618
Size: 761 1341 3618
Fragments arranged by size:
3618 1341 761
BclI T'GATC_A
Cuts at: 3240 3240
Size: 5720
BfrBI ATG'CAT
Cuts at: 2512 2512
Size: 5720
BglI GCCn_nnn'nGGC
Cuts at: 1358 1697 1358
Size: 339 5381
BglII A'GATC_T
Cuts at: 3235 5272 3235
Size: 2037 3683
BlpI GC'TnA_GC
Cuts at: 2832 2832
Size: 5720
Bme1580I G_kGCm'C
Cuts at: 255 646 658 1354 1445 1688 2048 3345 3438
Size: 391 12 696 91 243 360 1297 93
Cuts at: 3438 4494 255
Size: 1056 1481
Fragments arranged by size:
1481 1297 1056 696 391 360 243 93
91 12
BmgBI CAC'GTC
Cuts at: 659 659
Size: 5720
BmrI ACTGGGnnnn_n'
Cuts at: 752 774 1425 1485 1701 2932 3013 3348 5492
Size: 22 651 60 216 1231 81 335 2144
Cuts at: 5492 752
Size: 980
Fragments arranged by size:
2144 1231 980 651 335 216 81 60
22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 4087 2194
Size: 1893 3827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 523 544 827 883 1431 1774 1905 2613
Size: 15 21 283 56 548 343 131 708
Cuts at: 2613 508
Size: 3615
Fragments arranged by size:
3615 708 548 343 283 131 56 21
15
Bpu10I CC'TnA_GC
Cuts at: 607 1020 1138 1836 607
Size: 413 118 698 4491
Fragments arranged by size:
4491 698 413 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2896 3775 4267 4565 2896
Size: 879 492 298 4051
Fragments arranged by size:
4051 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1234 2186 5424 1234
Size: 952 3238 1530
Fragments arranged by size:
3238 1530 952
BsaAI yAC'GTr
Cuts at: 3073 3073
Size: 5720
BsaBI GATnn'nnATC
Cuts at: 6 3234 5271 6
Size: 3228 2037 455
Fragments arranged by size:
3228 2037 455
BsaHI Gr'CG_yC
Cuts at: 170 812 1678 2525 3151 3399 4101 5597 170
Size: 642 866 847 626 248 702 1496 293
Fragments arranged by size:
1496 866 847 702 642 626 293 248
BsaWI w'CCGG_w
Cuts at: 380 1324 2505 2690 2909 3430 4108 4382 4529
Size: 944 1181 185 219 521 678 274 147
Cuts at: 4529 4940 380
Size: 411 1160
Fragments arranged by size:
1181 1160 944 678 521 411 274 219
185 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2185 2215 2410 2440 2595 2625 2185
Size: 30 195 30 155 30 5280
Fragments arranged by size:
5280 195 155 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 709 1008 1251 1615 2045 709
Size: 299 243 364 430 4384
Fragments arranged by size:
4384 430 364 299 243
BseYI C'CCAG_C
Cuts at: 118 227 1603 1939 2097 2142 2369 2651 3145
Size: 109 1376 336 158 45 227 282 494
Cuts at: 3145 3903 4480 4976 118
Size: 758 577 496 862
Fragments arranged by size:
1376 862 758 577 496 494 336 282
227 158 109 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 673 723 1633 1642 2213 213
Size: 460 50 910 9 571 3720
Fragments arranged by size:
3720 910 571 460 50 9
BsiEI CG_ry'CG
Cuts at: 104 3262 3308 4516 104
Size: 3158 46 1208 1308
Fragments arranged by size:
3158 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1862 2153 2734 3512 3702 4494 1862
Size: 291 581 778 190 792 3088
Fragments arranged by size:
3088 792 778 581 291 190
BsmBI CGTCTCn'nnnn_
Cuts at: 197 446 197
Size: 249 5471
Bsp1286I G_dGCh'C
Cuts at: 255 646 658 1204 1354 1445 1688 1862 2048
Size: 391 12 546 150 91 243 174 186
Cuts at: 2048 2153 2203 2336 2734 3345 3438 3512 3702
Size: 105 50 133 398 611 93 74 190
Cuts at: 3702 3764 4494 255
Size: 62 730 1481
Fragments arranged by size:
1481 730 611 546 398 391 243 190
186 174 150 133 105 93 91 74
62 50 12
BspEI T'CCGG_A
Cuts at: 2505 2690 4940 2505
Size: 185 2250 3285
Fragments arranged by size:
3285 2250 185
BspHI T'CATG_A
Cuts at: 4896 4896
Size: 5720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 368 994 1098 1157 2460 2770 2984 3286
Size: 87 626 104 59 1303 310 214 302
Cuts at: 3286 3667 281
Size: 381 2334
Fragments arranged by size:
2334 1303 626 381 310 302 214 104
87 59
BsrBI CCG'CTC
Cuts at: 50 511 1040 1178 1569 4012 4066 5550 5715
Size: 461 529 138 391 2443 54 1484 165
Cuts at: 5715 50
Size: 55
Fragments arranged by size:
2443 1484 529 461 391 165 138 55
54
BsrDI GCAATG_nn'
Cuts at: 1109 3632 1109
Size: 2523 3197
BsrFI r'CCGG_y
Cuts at: 148 285 380 431 2008 3718 3899 4108 148
Size: 137 95 51 1577 1710 181 209 1760
Fragments arranged by size:
1760 1710 1577 209 181 137 95 51
BssHII G'CGCG_C
Cuts at: 749 749
Size: 5720
BssSI C'ACGA_G
Cuts at: 691 1246 3991 4349 691
Size: 555 2745 358 2062
Fragments arranged by size:
2745 2062 555 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1104 1620 1656 2181 3097 1104
Size: 516 36 525 916 3727
Fragments arranged by size:
3727 916 525 516 36
BstBI TT'CG_AA
Cuts at: 4081 4081
Size: 5720
BstEII G'GTnAC_C
Cuts at: 1315 1315
Size: 5720
BstXI CCAn_nnnn'nTGG
Cuts at: 874 1809 874
Size: 935 4785
BstYI r'GATC_y
Cuts at: 1 1763 2406 2745 3212 3235 3570 3816 4828
Size: 1762 643 339 467 23 335 246 1012
Cuts at: 4828 4914 4926 5272 1
Size: 86 12 346 449
Fragments arranged by size:
1762 1012 643 467 449 346 339 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1205 1205
Size: 5720
BtgI C'CryG_G
Cuts at: 107 369 1124 1310 1551 2162 107
Size: 262 755 186 241 611 3665
Fragments arranged by size:
3665 755 611 262 241 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3670 3851 4014 3670
Size: 181 163 5376
Fragments arranged by size:
5376 181 163
BtsI GCAGTG_nn'
Cuts at: 253 1286 2003 2963 253
Size: 1033 717 960 3010
Fragments arranged by size:
3010 1033 960 717
ClaI AT'CG_AT
Cuts at: 326 326
Size: 5720
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1091 1126 1091
Size: 35 5685
DraI TTT'AAA
Cuts at: 2719 2719
Size: 5720
DrdI GACnn_nn'nnGTC
Cuts at: 2373 3426 4284 2373
Size: 1053 858 3809
Fragments arranged by size:
3809 1053 858
EaeI y'GGCC_r
Cuts at: 1031 1163 2397 2446 2564 3305 3479 3870 3897
Size: 132 1234 49 118 741 174 391 27
Cuts at: 3897 5242 1031
Size: 1345 1509
Fragments arranged by size:
1509 1345 1234 741 391 174 132 118
49 27
EagI C'GGCC_G
Cuts at: 3305 3305
Size: 5720
EarI CTCTTCn'nnn_
Cuts at: 1269 1478 1718 1867 2120 3743 3953 1269
Size: 209 240 149 253 1623 210 3036
Fragments arranged by size:
3036 1623 253 240 210 209 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 118 1583 1894 3928 4238 4384 5530 118
Size: 1465 311 2034 310 146 1146 308
Fragments arranged by size:
2034 1465 1146 311 310 308 146
EcoICRI GAG'CTC
Cuts at: 2732 2732
Size: 5720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 523 544 827 883 1431 1561 1774 1905
Size: 15 21 283 56 548 130 213 131
Cuts at: 1905 2047 2613 3545 3977 4724 4992 508
Size: 142 566 932 432 747 268 1236
Fragments arranged by size:
1236 932 747 566 548 432 283 268
213 142 131 130 56 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 806 1096 806
Size: 290 5430
EcoO109I rG'GnC_Cy
Cuts at: 270 336 357 564 642 643 904 951 1233
Size: 66 21 207 78 1 261 47 282
Cuts at: 1233 1350 1699 2031 2324 2859 270
Size: 117 349 332 293 535 3131
Fragments arranged by size:
3131 535 349 332 293 282 261 207
117 78 66 47 21 1
EcoRI G'AATT_C
Cuts at: 2724 2724
Size: 5720
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3739 3739
Size: 5720
FspI TGC'GCA
Cuts at: 1292 3501 1292
Size: 2209 3511
HaeII r_GCGC'y
Cuts at: 624 815 1521 1788 1843 2268 2364 3154 3402
Size: 191 706 267 55 425 96 790 248
Cuts at: 3402 4424 5403 624
Size: 1022 979 941
Fragments arranged by size:
1022 979 941 790 706 425 267 248
191 96 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1791 1823 2185 2217 2410 2442 3562 3592 3594
Size: 32 362 32 193 32 1120 30 2
Cuts at: 3594 3624 1791
Size: 30 3887
Fragments arranged by size:
3887 1120 362 193 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2501 2759 2501
Size: 258 5462
KasI G'GCGC_C
Cuts at: 811 3150 3398 811
Size: 2339 248 3133
Fragments arranged by size:
3133 2339 248
KpnI G_GTAC'C
Cuts at: 381 2740 381
Size: 2359 3361
MluI A'CGCG_T
Cuts at: 5153 5153
Size: 5720
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4391 4575 4391
Size: 184 5536
MscI TGG'CCA
Cuts at: 1165 2399 2448 2566 3481 1165
Size: 1234 49 118 915 3404
Fragments arranged by size:
3404 1234 915 118 49
MslI CAynn'nnrTG
Cuts at: 661 1327 3709 3836 661
Size: 666 2382 127 2545
Fragments arranged by size:
2545 2382 666 127
MspA1I CmG'CkG
Cuts at: 197 371 720 792 1607 1647 1653 2139 2373
Size: 174 349 72 815 40 6 486 234
Cuts at: 2373 2541 2831 3145 3505 4518 4763 197
Size: 168 290 314 360 1013 245 1154
Fragments arranged by size:
1154 1013 815 486 360 349 314 290
245 234 174 168 72 40 6
NaeI GCC'GGC
Cuts at: 287 2010 3901 287
Size: 1723 1891 2106
Fragments arranged by size:
2106 1891 1723
NarI GG'CG_CC
Cuts at: 812 3151 3399 812
Size: 2339 248 3133
Fragments arranged by size:
3133 2339 248
NcoI C'CATG_G
Cuts at: 107 1551 2162 107
Size: 1444 611 3665
Fragments arranged by size:
3665 1444 611
NgoMIV G'CCGG_C
Cuts at: 285 2008 3899 285
Size: 1723 1891 2106
Fragments arranged by size:
2106 1891 1723
NsiI A_TGCA'T
Cuts at: 2514 2514
Size: 5720
NspI r_CATG'y
Cuts at: 262 929 2516 2773 3273 4180 262
Size: 667 1587 257 500 907 1802
Fragments arranged by size:
1802 1587 907 667 500 257
PasI CC'CwG_GG
Cuts at: 295 332 471 584 596 2520 295
Size: 37 139 113 12 1924 3495
Fragments arranged by size:
3495 1924 139 113 37 12
PciI A'CATG_T
Cuts at: 4176 4176
Size: 5720
PflMI CCAn_nnn'nTGG
Cuts at: 1437 2097 1437
Size: 660 5060
PfoI T'CCnGG_A
Cuts at: 560 765 1253 1720 1987 560
Size: 205 488 467 267 4293
Fragments arranged by size:
4293 488 467 267 205
PmeI GTTT'AAAC
Cuts at: 2719 2719
Size: 5720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4859 4891 4859
Size: 32 5688
PpuMI rG'GwC_Cy
Cuts at: 270 336 357 564 904 1233 2324 270
Size: 66 21 207 340 329 1091 3666
Fragments arranged by size:
3666 1091 340 329 207 66 21
PshAI GACnn'nnGTC
Cuts at: 538 538
Size: 5720
PspOMI G'GGCC_C
Cuts at: 642 1350 642
Size: 708 5012
PstI C_TGCA'G
Cuts at: 1100 1174 1652 1781 2017 2189 2767 3452 1100
Size: 74 478 129 236 172 578 685 3368
Fragments arranged by size:
3368 685 578 478 236 172 129 74
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5720
PvuII CAG'CTG
Cuts at: 197 720 792 1607 1647 1653 2373 3145 3505
Size: 523 72 815 40 6 720 772 360
Cuts at: 3505 197
Size: 2412
Fragments arranged by size:
2412 815 772 720 523 360 72 40
6
RsrII CG'GwC_CG
Cuts at: 3915 3915
Size: 5720
SacI G_AGCT'C
Cuts at: 2734 2734
Size: 5720
SacII CC_GC'GG
Cuts at: 372 372
Size: 5720
SalI G'TCGA_C
Cuts at: 2757 2757
Size: 5720
SanDI GG'GwC_CC
Cuts at: 336 1233 336
Size: 897 4823
SapI GCTCTTCn'nnn_
Cuts at: 1867 3743 3953 1867
Size: 1876 210 3634
Fragments arranged by size:
3634 1876 210
SbfI CC_TGCA'GG
Cuts at: 2767 2767
Size: 5720
ScaI AGT'ACT
Cuts at: 5237 5237
Size: 5720
SfcI C'TryA_G
Cuts at: 33 1096 1170 1227 1648 1777 2013 2185 2763
Size: 1063 74 57 421 129 236 172 578
Cuts at: 2763 3448 4441 4632 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 1063 993 685 578 421 236 191
172 129 74 57
SfoI GGC'GCC
Cuts at: 813 3152 3400 813
Size: 2339 248 3133
Fragments arranged by size:
3133 2339 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5720
SmaI CCC'GGG
Cuts at: 355 1155 2337 2742 355
Size: 800 1182 405 3333
Fragments arranged by size:
3333 1182 800 405
SmlI C'TyrA_G
Cuts at: 2875 3790 4282 4544 2875
Size: 915 492 262 4051
Fragments arranged by size:
4051 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5720
SphI G_CATG'C
Cuts at: 2516 2773 3273 2516
Size: 257 500 4963
Fragments arranged by size:
4963 500 257
StuI AGG'CCT
Cuts at: 736 1853 2243 2626 736
Size: 1117 390 383 3830
Fragments arranged by size:
3830 1117 390 383
StyI C'CwwG_G
Cuts at: 107 156 266 873 1551 2162 2854 3208 107
Size: 49 110 607 678 611 692 354 2619
Fragments arranged by size:
2619 692 678 611 607 354 110 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1303 1972 2088 2098 2134 3303 3436 3895 4104
Size: 669 116 10 36 1169 133 459 209
Cuts at: 4104 1303
Size: 2919
Fragments arranged by size:
2919 1169 669 459 209 133 116 36
10
TatI w'GTAC_w
Cuts at: 5235 5235
Size: 5720
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 2595 2627 5237 5269 262
Size: 32 2301 32 2610 32 713
Fragments arranged by size:
2610 2301 713 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3517 3517
Size: 5720
XbaI T'CTAG_A
Cuts at: 2751 2751
Size: 5720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 568 1851 3215 568
Size: 1283 1364 3073
Fragments arranged by size:
3073 1364 1283
XmaI C'CCGG_G
Cuts at: 353 1153 2335 2740 353
Size: 800 1182 405 3333
Fragments arranged by size:
3333 1182 800 405
ZraI GAC'GTC
Cuts at: 171 171
Size: 5720
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AloI AscI AvrII BaeI BmtI BsiWI
BsmI BsrGI BstZ17I DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PmlI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI