Restriction map for
pF1KB0432
(Circular) (Six-base) MAPSORT of: pF1KB0432.seq Check: 5899 from: 1 to: 5675
pF1KB0432 5675 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1407 1509 1790 1407
Size: 102 281 5292
Fragments arranged by size:
5292 281 102
AccI GT'mk_AC
Cuts at: 1724 2651 2713 1724
Size: 927 62 4686
Fragments arranged by size:
4686 927 62
Acc65I G'GTAC_C
Cuts at: 2556 2691 2556
Size: 135 5540
AclI AA'CG_TT
Cuts at: 5515 5515
Size: 5675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 2038 2137 2166 2455 3500 3932 4679 4947
Size: 1014 99 29 289 1045 432 747 268
Cuts at: 4947 1024
Size: 1752
Fragments arranged by size:
1752 1045 1014 747 432 289 268 99
29
AflIII A'CryG_T
Cuts at: 2027 3027 4131 5108 2027
Size: 1000 1104 977 2594
Fragments arranged by size:
2594 1104 1000 977
AgeI A'CCGG_T
Cuts at: 1141 4063 1141
Size: 2922 2753
AleI CACnn'nnGTG
Cuts at: 1142 1247 1958 1142
Size: 105 711 4859
Fragments arranged by size:
4859 711 105
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 598 630 598
Size: 32 5643
AlwNI CAG_nnn'CTG
Cuts at: 199 492 717 819 1344 2449 2980 4547 199
Size: 293 225 102 525 1105 531 1567 1327
Fragments arranged by size:
1567 1327 1105 531 525 293 225 102
ApaI G_GGCC'C
Cuts at: 583 724 2626 583
Size: 141 1902 3632
Fragments arranged by size:
3632 1902 141
ApaLI G'TGCA_C
Cuts at: 1666 4445 1666
Size: 2779 2896
ApoI r'AATT_y
Cuts at: 1465 2679 5028 5284 1465
Size: 1214 2349 256 1856
Fragments arranged by size:
2349 1856 1214 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5675
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
AvaI C'yCGr_G
Cuts at: 1956 2461 2695 1956
Size: 505 234 4936
Fragments arranged by size:
4936 505 234
BamHI G'GATC_C
Cuts at: 2700 2700
Size: 5675
BanI G'GyrC_C
Cuts at: 849 1024 1252 2457 2556 2691 3105 3353 3388
Size: 175 228 1205 99 135 414 248 35
Cuts at: 3388 849
Size: 3136
Fragments arranged by size:
3136 1205 414 248 228 175 135 99
35
BanII G_rGCy'C
Cuts at: 583 724 2045 2584 2626 2689 3719 583
Size: 141 1321 539 42 63 1030 2539
Fragments arranged by size:
2539 1321 1030 539 141 63 42
BbeI G_GCGC'C
Cuts at: 3109 3357 3109
Size: 248 5427
BbsI GAAGACnn'nnnn_
Cuts at: 1012 1012
Size: 5675
BbvCI CC'TCA_GC
Cuts at: 689 2570 689
Size: 1881 3794
BciVI GTATCCnnnnn_n'
Cuts at: 3573 4334 5675 3573
Size: 761 1341 3573
Fragments arranged by size:
3573 1341 761
BclI T'GATC_A
Cuts at: 3195 3195
Size: 5675
BfrBI ATG'CAT
Cuts at: 612 612
Size: 5675
BglII A'GATC_T
Cuts at: 446 3190 5227 446
Size: 2744 2037 894
Fragments arranged by size:
2744 2037 894
BlpI GC'TnA_GC
Cuts at: 1609 2787 1609
Size: 1178 4497
Bme1580I G_kGCm'C
Cuts at: 470 583 623 724 973 1551 1670 2196 2462
Size: 113 40 101 249 578 119 526 266
Cuts at: 2462 2626 2648 3300 3393 4449 470
Size: 164 22 652 93 1056 1696
Fragments arranged by size:
1696 1056 652 578 526 266 249 164
119 113 101 93 40 22
BmrI ACTGGGnnnn_n'
Cuts at: 829 2428 2887 2968 3303 5447 829
Size: 1599 459 81 335 2144 1057
Fragments arranged by size:
2144 1599 1057 459 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4042 4042
Size: 5675
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 857 977 1220 1600 1792 2446 154
Size: 703 120 243 380 192 654 3383
Fragments arranged by size:
3383 703 654 380 243 192 120
Bpu10I CC'TnA_GC
Cuts at: 689 2570 689
Size: 1881 3794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1471 2851 3730 4222 4520 1057
Size: 414 1380 879 492 298 2212
Fragments arranged by size:
2212 1380 879 492 414 298
BsaI GGTCTCn'nnnn_
Cuts at: 5379 5379
Size: 5675
BsaAI yAC'GTr
Cuts at: 3028 3028
Size: 5675
BsaBI GATnn'nnATC
Cuts at: 6 3189 5226 6
Size: 3183 2037 455
Fragments arranged by size:
3183 2037 455
BsaHI Gr'CG_yC
Cuts at: 732 1854 3106 3354 4056 5552 732
Size: 1122 1252 248 702 1496 855
Fragments arranged by size:
1496 1252 1122 855 702 248
BsaWI w'CCGG_w
Cuts at: 1141 2864 3385 4063 4337 4484 4895 1141
Size: 1723 521 678 274 147 411 1921
Fragments arranged by size:
1921 1723 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 624 654 1660 1690 1738 1768 2116 2146 624
Size: 30 1006 30 48 30 348 30 4153
Fragments arranged by size:
4153 1006 348 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 638 641 650 1618 2367 2443 638
Size: 3 9 968 749 76 3870
Fragments arranged by size:
3870 968 749 76 9 3
BseYI C'CCAG_C
Cuts at: 622 635 790 1300 1459 1502 2449 2577 3100
Size: 13 155 510 159 43 947 128 523
Cuts at: 3100 3858 4435 4931 622
Size: 758 577 496 1366
Fragments arranged by size:
1366 947 758 577 523 510 496 159
155 128 43 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 613 2190 517
Size: 96 1577 4002
Fragments arranged by size:
4002 1577 96
BsiEI CG_ry'CG
Cuts at: 104 2107 3217 3263 4471 104
Size: 2003 1110 46 1208 1308
Fragments arranged by size:
2003 1308 1208 1110 46
BsiHKAI G_wGCw'C
Cuts at: 530 1081 1586 1670 1778 2689 3467 3657 4449
Size: 551 505 84 108 911 778 190 792
Cuts at: 4449 530
Size: 1756
Fragments arranged by size:
1756 911 792 778 551 505 190 108
84
BsmI GAATG_Cn'
Cuts at: 348 612 348
Size: 264 5411
BsmBI CGTCTCn'nnnn_
Cuts at: 2308 2308
Size: 5675
Bsp1286I G_dGCh'C
Cuts at: 470 530 583 623 724 973 1081 1551 1586
Size: 60 53 40 101 249 108 470 35
Cuts at: 1586 1670 1778 2045 2196 2462 2584 2626 2648
Size: 84 108 267 151 266 122 42 22
Cuts at: 2648 2689 3300 3393 3467 3657 3719 4449 470
Size: 41 611 93 74 190 62 730 1696
Fragments arranged by size:
1696 730 611 470 267 266 249 190
151 122 108 108 101 93 84 74
62 60 53 42 41 40 35 22
BspEI T'CCGG_A
Cuts at: 4895 4895
Size: 5675
BspHI T'CATG_A
Cuts at: 4851 4851
Size: 5675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1124 1407 1509 1790 1856 1874 2336 2725 2939
Size: 283 102 281 66 18 462 389 214
Cuts at: 2939 3241 3622 1124
Size: 302 381 3177
Fragments arranged by size:
3177 462 389 381 302 283 281 214
102 66 18
BsrBI CCG'CTC
Cuts at: 50 3967 4021 5505 5670 50
Size: 3917 54 1484 165 55
Fragments arranged by size:
3917 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1163 3587 1163
Size: 2424 3251
BsrFI r'CCGG_y
Cuts at: 1141 2102 3673 3854 4063 1141
Size: 961 1571 181 209 2753
Fragments arranged by size:
2753 1571 961 209 181
BsrGI T'GTAC_A
Cuts at: 767 896 1523 767
Size: 129 627 4919
Fragments arranged by size:
4919 627 129
BssHII G'CGCG_C
Cuts at: 2214 2214
Size: 5675
BssSI C'ACGA_G
Cuts at: 2038 3946 4304 2038
Size: 1908 358 3409
Fragments arranged by size:
3409 1908 358
BstAPI GCAn_nnn'nTGC
Cuts at: 618 1782 3052 618
Size: 1164 1270 3241
Fragments arranged by size:
3241 1270 1164
BstBI TT'CG_AA
Cuts at: 4036 4036
Size: 5675
BstXI CCAn_nnnn'nTGG
Cuts at: 1419 2520 1419
Size: 1101 4574
BstYI r'GATC_y
Cuts at: 1 446 809 2700 3167 3190 3525 3771 4783
Size: 445 363 1891 467 23 335 246 1012
Cuts at: 4783 4869 4881 5227 1
Size: 86 12 346 449
Fragments arranged by size:
1891 1012 467 449 445 363 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1763 1763
Size: 5675
BtgI C'CryG_G
Cuts at: 107 1869 107
Size: 1762 3913
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 533 3625 3806 3969 533
Size: 3092 181 163 2239
Fragments arranged by size:
3092 2239 181 163
BtsI GCAGTG_nn'
Cuts at: 667 1815 2385 2918 667
Size: 1148 570 533 3424
Fragments arranged by size:
3424 1148 570 533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 726 761 726
Size: 35 5640
DraI TTT'AAA
Cuts at: 2674 2674
Size: 5675
DraIII CAC_nnn'GTG
Cuts at: 1227 1227
Size: 5675
DrdI GACnn_nn'nnGTC
Cuts at: 1720 3381 4239 1720
Size: 1661 858 3156
Fragments arranged by size:
3156 1661 858
EaeI y'GGCC_r
Cuts at: 183 356 371 977 1173 2104 3260 3434 3825
Size: 173 15 606 196 931 1156 174 391
Cuts at: 3825 3852 5197 183
Size: 27 1345 661
Fragments arranged by size:
1345 1156 931 661 606 391 196 174
173 27 15
EagI C'GGCC_G
Cuts at: 2104 3260 2104
Size: 1156 4519
EarI CTCTTCn'nnn_
Cuts at: 1385 2014 3698 3908 1385
Size: 629 1684 210 3152
Fragments arranged by size:
3152 1684 629 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2614 3883 4193 4339 5485 2614
Size: 1269 310 146 1146 2804
Fragments arranged by size:
2804 1269 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2687 2687
Size: 5675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 857 977 1024 1220 1600 1792 2038 2137
Size: 703 120 47 196 380 192 246 99
Cuts at: 2137 2166 2446 2455 3500 3932 4679 4947 154
Size: 29 280 9 1045 432 747 268 882
Fragments arranged by size:
1045 882 747 703 432 380 280 268
246 196 192 120 99 47 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 496 496
Size: 5675
EcoO109I rG'GnC_Cy
Cuts at: 129 423 579 580 721 1767 2325 2622 2814
Size: 294 156 1 141 1046 558 297 192
Cuts at: 2814 129
Size: 2990
Fragments arranged by size:
2990 1046 558 297 294 192 156 141
1
EcoRI G'AATT_C
Cuts at: 2679 2679
Size: 5675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1929 2502 3694 1929
Size: 573 1192 3910
Fragments arranged by size:
3910 1192 573
FspI TGC'GCA
Cuts at: 3456 3456
Size: 5675
HaeII r_GCGC'y
Cuts at: 962 3109 3357 4379 5358 962
Size: 2147 248 1022 979 1279
Fragments arranged by size:
2147 1279 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1662 1779 1811 3517 3547 3549 3579 1630
Size: 32 117 32 1706 30 2 30 3726
Fragments arranged by size:
3726 1706 117 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1746 2714 1746
Size: 968 4707
KasI G'GCGC_C
Cuts at: 3105 3353 3105
Size: 248 5427
KpnI G_GTAC'C
Cuts at: 2560 2695 2560
Size: 135 5540
MluI A'CGCG_T
Cuts at: 5108 5108
Size: 5675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 229 2609 4346 4530 229
Size: 2380 1737 184 1374
Fragments arranged by size:
2380 1737 1374 184
MscI TGG'CCA
Cuts at: 185 358 373 979 3436 185
Size: 173 15 606 2457 2424
Fragments arranged by size:
2457 2424 606 173 15
MslI CAynn'nnrTG
Cuts at: 463 511 1142 1247 1511 1958 2518 3664 3791
Size: 48 631 105 264 447 560 1146 127
Cuts at: 3791 463
Size: 2347
Fragments arranged by size:
2347 1146 631 560 447 264 127 105
48
MspA1I CmG'CkG
Cuts at: 412 1381 1619 1877 2243 2297 2786 3100 3460
Size: 969 238 258 366 54 489 314 360
Cuts at: 3460 4473 4718 412
Size: 1013 245 1369
Fragments arranged by size:
1369 1013 969 489 366 360 314 258
245 238 54
NaeI GCC'GGC
Cuts at: 3856 3856
Size: 5675
NarI GG'CG_CC
Cuts at: 3106 3354 3106
Size: 248 5427
NcoI C'CATG_G
Cuts at: 107 107
Size: 5675
NgoMIV G'CCGG_C
Cuts at: 3854 3854
Size: 5675
NsiI A_TGCA'T
Cuts at: 614 614
Size: 5675
NspI r_CATG'y
Cuts at: 612 1172 1215 1945 2031 2728 3228 4135 612
Size: 560 43 730 86 697 500 907 2152
Fragments arranged by size:
2152 907 730 697 560 500 86 43
PasI CC'CwG_GG
Cuts at: 576 727 576
Size: 151 5524
PciI A'CATG_T
Cuts at: 2027 4131 2027
Size: 2104 3571
PflMI CCAn_nnn'nTGG
Cuts at: 246 492 717 819 864 246
Size: 246 225 102 45 5057
Fragments arranged by size:
5057 246 225 102 45
PfoI T'CCnGG_A
Cuts at: 1037 1236 1037
Size: 199 5476
PmeI GTTT'AAAC
Cuts at: 2674 2674
Size: 5675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1981 2013 4814 4846 1981
Size: 32 2801 32 2810
Fragments arranged by size:
2810 2801 32 32
PpuMI rG'GwC_Cy
Cuts at: 2325 2325
Size: 5675
PspOMI G'GGCC_C
Cuts at: 579 720 2622 579
Size: 141 1902 3632
Fragments arranged by size:
3632 1902 141
PstI C_TGCA'G
Cuts at: 1050 1121 1787 2089 2609 2722 3407 1050
Size: 71 666 302 520 113 685 3318
Fragments arranged by size:
3318 685 666 520 302 113 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5675
PvuII CAG'CTG
Cuts at: 1381 3100 3460 1381
Size: 1719 360 3596
Fragments arranged by size:
3596 1719 360
RsrII CG'GwC_CG
Cuts at: 3870 3870
Size: 5675
SacI G_AGCT'C
Cuts at: 2689 2689
Size: 5675
SalI G'TCGA_C
Cuts at: 2712 2712
Size: 5675
SapI GCTCTTCn'nnn_
Cuts at: 3698 3908 3698
Size: 210 5465
SbfI CC_TGCA'GG
Cuts at: 2722 2722
Size: 5675
ScaI AGT'ACT
Cuts at: 1675 5192 1675
Size: 3517 2158
SfcI C'TryA_G
Cuts at: 33 1046 1117 1783 2085 2605 2718 3403 4396
Size: 1013 71 666 302 520 113 685 993
Cuts at: 4396 4587 33
Size: 191 1121
Fragments arranged by size:
1121 1013 993 685 666 520 302 191
113 71
SfoI GGC'GCC
Cuts at: 3107 3355 3107
Size: 248 5427
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
SgrAI Cr'CCGG_yG
Cuts at: 1141 1141
Size: 5675
SmaI CCC'GGG
Cuts at: 2697 2697
Size: 5675
SmlI C'TyrA_G
Cuts at: 1072 1486 2830 3745 4237 4499 1072
Size: 414 1344 915 492 262 2248
Fragments arranged by size:
2248 1344 915 492 414 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5675
SphI G_CATG'C
Cuts at: 1945 2728 3228 1945
Size: 783 500 4392
Fragments arranged by size:
4392 783 500
StuI AGG'CCT
Cuts at: 1369 2662 1369
Size: 1293 4382
StyI C'CwwG_G
Cuts at: 107 186 2258 2809 3163 107
Size: 79 2072 551 354 2619
Fragments arranged by size:
2619 2072 551 354 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 420 649 1882 2512 3258 3391 3850 4059 420
Size: 229 1233 630 746 133 459 209 2036
Fragments arranged by size:
2036 1233 746 630 459 229 209 133
TatI w'GTAC_w
Cuts at: 767 896 1523 1673 5190 767
Size: 129 627 150 3517 1252
Fragments arranged by size:
3517 1252 627 150 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 624 656 1660 1692 2116 2148 5192 5224 624
Size: 32 1004 32 424 32 3044 32 1075
Fragments arranged by size:
3044 1075 1004 424 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3472 3472
Size: 5675
XbaI T'CTAG_A
Cuts at: 2706 2706
Size: 5675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 2592 3170 366
Size: 2226 578 2871
Fragments arranged by size:
2871 2226 578
XmaI C'CCGG_G
Cuts at: 2695 2695
Size: 5675
XmnI GAAnn'nnTTC
Cuts at: 289 1153 1912 289
Size: 864 759 4052
Fragments arranged by size:
4052 864 759
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsiWI BstEII BstZ17I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XhoI ZraI