Restriction map for
pF1KB0069
(Circular) (Six-base) MAPSORT of: pF1KB0069.seq Check: 2352 from: 1 to: 10853
pF1KB0069 10853 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 841 841
Size: 10853
AccI GT'mk_AC
Cuts at: 7891 7891
Size: 10853
Acc65I G'GTAC_C
Cuts at: 7869 7869
Size: 10853
AclI AA'CG_TT
Cuts at: 10693 10693
Size: 10853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 1249 2005 2191 2315 2354 2399 2641 2728
Size: 890 756 186 124 39 45 242 87
Cuts at: 2728 4478 4822 5242 6283 7054 8678 9110 9463
Size: 1750 344 420 1041 771 1624 432 353
Cuts at: 9463 10125 359
Size: 662 1087
Fragments arranged by size:
1750 1624 1087 1041 890 771 756 662
432 420 353 344 242 186 124 87
45 39
AflII C'TTAA_G
Cuts at: 4924 5506 4924
Size: 582 10271
AflIII A'CryG_T
Cuts at: 489 1012 2668 8205 10005 10286 489
Size: 523 1656 5537 1800 281 1056
Fragments arranged by size:
5537 1800 1656 1056 523 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 7843 7843
Size: 10853
AlwNI CAG_nnn'CTG
Cuts at: 847 1513 1911 4380 5340 6738 8158 9596 847
Size: 666 398 2469 960 1398 1420 1438 2104
Fragments arranged by size:
2469 2104 1438 1420 1398 960 666 398
ApaLI G'TGCA_C
Cuts at: 9691 9691
Size: 10853
ApoI r'AATT_y
Cuts at: 478 801 977 987 1473 1714 2485 2493 2599
Size: 323 176 10 486 241 771 8 106
Cuts at: 2599 2990 3012 3052 3516 4039 5469 5640 5646
Size: 391 22 40 464 523 1430 171 6
Cuts at: 5646 5870 5891 6130 6238 6322 6467 6514 6890
Size: 224 21 239 108 84 145 47 376
Cuts at: 6890 6906 7174 7409 7554 7632 7857 10206 10462
Size: 16 268 235 145 78 225 2349 256
Cuts at: 10462 478
Size: 869
Fragments arranged by size:
2349 1430 869 771 523 486 464 391
376 323 268 256 241 239 235 225
224 176 171 145 145 108 106 84
78 47 40 22 21 16 10 8
6
AseI AT'TA_AT
Cuts at: 20 2972 3503 4511 20
Size: 2952 531 1008 6362
Fragments arranged by size:
6362 2952 1008 531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10853
AvaI C'yCGr_G
Cuts at: 4747 7873 4747
Size: 3126 7727
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1022 1055 7268 7301 1022
Size: 33 6213 33 4574
Fragments arranged by size:
6213 4574 33 33
BamHI G'GATC_C
Cuts at: 7878 7878
Size: 10853
BanI G'GyrC_C
Cuts at: 1376 6811 7869 8283 8531 8566 1376
Size: 5435 1058 414 248 35 3663
Fragments arranged by size:
5435 3663 1058 414 248 35
BanII G_rGCy'C
Cuts at: 1212 2342 7867 8897 1212
Size: 1130 5525 1030 3168
Fragments arranged by size:
5525 3168 1130 1030
BbeI G_GCGC'C
Cuts at: 8287 8535 8287
Size: 248 10605
BbsI GAAGACnn'nnnn_
Cuts at: 1802 1892 2632 3247 3820 5437 6799 1802
Size: 90 740 615 573 1617 1362 5856
Fragments arranged by size:
5856 1617 1362 740 615 573 90
BbvCI CC'TCA_GC
Cuts at: 2308 7307 2308
Size: 4999 5854
BciVI GTATCCnnnnn_n'
Cuts at: 801 8751 9807 10853 801
Size: 7950 1056 1046 801
Fragments arranged by size:
7950 1056 1046 801
BclI T'GATC_A
Cuts at: 911 2478 6078 8373 911
Size: 1567 3600 2295 3391
Fragments arranged by size:
3600 3391 2295 1567
BfrBI ATG'CAT
Cuts at: 5217 5376 6939 5217
Size: 159 1563 9131
Fragments arranged by size:
9131 1563 159
BglII A'GATC_T
Cuts at: 5077 5787 7119 8368 10405 5077
Size: 710 1332 1249 2037 5525
Fragments arranged by size:
5525 2037 1332 1249 710
BlpI GC'TnA_GC
Cuts at: 7094 7965 7094
Size: 871 9982
Bme1580I G_kGCm'C
Cuts at: 6816 8478 8571 9695 6816
Size: 1662 93 1124 7974
Fragments arranged by size:
7974 1662 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3874 8065 8146 8481 10625 3874
Size: 4191 81 335 2144 4102
Fragments arranged by size:
4191 4102 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1626 5161 5770 7267 9220 1626
Size: 3535 609 1497 1953 3259
Fragments arranged by size:
3535 3259 1953 1497 609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1019 1051 1999 2110 3976 5761 967
Size: 52 32 948 111 1866 1785 6059
Fragments arranged by size:
6059 1866 1785 948 111 52 32
Bpu10I CC'TnA_GC
Cuts at: 1211 2308 4024 4355 7307 1211
Size: 1097 1716 331 2952 4757
Fragments arranged by size:
4757 2952 1716 1097 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 2563 2801 2903 2962 3598 4886 5131 5740
Size: 1716 238 102 59 636 1288 245 609
Cuts at: 5740 6997 7719 8029 8908 9622 9920 847
Size: 1257 722 310 879 714 298 1780
Fragments arranged by size:
1780 1716 1288 1257 879 722 714 636
609 310 298 245 238 102 59
BsaI GGTCTCn'nnnn_
Cuts at: 3674 4844 4987 5132 5171 5741 10557 3674
Size: 1170 143 145 39 570 4816 3970
Fragments arranged by size:
4816 3970 1170 570 145 143 39
BsaAI yAC'GTr
Cuts at: 490 1015 2671 8206 490
Size: 525 1656 5535 3137
Fragments arranged by size:
5535 3137 1656 525
BsaBI GATnn'nnATC
Cuts at: 6 8367 10404 6
Size: 8361 2037 455
Fragments arranged by size:
8361 2037 455
BsaHI Gr'CG_yC
Cuts at: 8284 8532 9234 10730 8284
Size: 248 702 1496 8407
Fragments arranged by size:
8407 1496 702 248
BsaWI w'CCGG_w
Cuts at: 8042 8563 9241 9652 9799 10073 8042
Size: 521 678 411 147 274 8822
Fragments arranged by size:
8822 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 965 995 1017 1047 4945 4975 965
Size: 30 22 30 3898 30 6843
Fragments arranged by size:
6843 3898 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1201 2560 5131 5209 5740 7803 1201
Size: 1359 2571 78 531 2063 4251
Fragments arranged by size:
4251 2571 2063 1359 531 78
BseYI C'CCAG_C
Cuts at: 8278 9036 9701 10109 8278
Size: 758 665 408 9022
Fragments arranged by size:
9022 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1008
Size: 10853
BsiEI CG_ry'CG
Cuts at: 104 151 8395 8441 9671 104
Size: 47 8244 46 1230 1286
Fragments arranged by size:
8244 1286 1230 47 46
BsiHKAI G_wGCw'C
Cuts at: 2342 5231 6037 7867 8645 8835 9695 2342
Size: 2889 806 1830 778 190 860 3500
Fragments arranged by size:
3500 2889 1830 860 806 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1195 1195
Size: 10853
Bsp1286I G_dGCh'C
Cuts at: 1212 2342 5231 6037 6816 7867 8478 8571 8645
Size: 1130 2889 806 779 1051 611 93 74
Cuts at: 8645 8835 8897 9695 1212
Size: 190 62 798 2370
Fragments arranged by size:
2889 2370 1130 1051 806 798 779 611
190 93 74 62
BspHI T'CATG_A
Cuts at: 438 3888 4332 5664 6251 7020 9285 438
Size: 3450 444 1332 587 769 2265 2006
Fragments arranged by size:
3450 2265 2006 1332 769 587 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 7903 8117 8419 8800 841
Size: 7062 214 302 381 2894
Fragments arranged by size:
7062 2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 9145 9199 10683 10848 50
Size: 9095 54 1484 165 55
Fragments arranged by size:
9095 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1223 8765 1223
Size: 7542 3311
BsrFI r'CCGG_y
Cuts at: 8851 9032 8851
Size: 181 10672
BsrGI T'GTAC_A
Cuts at: 3468 7353 3468
Size: 3885 6968
BssSI C'ACGA_G
Cuts at: 9124 9832 9124
Size: 708 10145
BstAPI GCAn_nnn'nTGC
Cuts at: 6811 8230 6811
Size: 1419 9434
BstBI TT'CG_AA
Cuts at: 9214 9214
Size: 10853
BstEII G'GTnAC_C
Cuts at: 1311 1311
Size: 10853
BstXI CCAn_nnnn'nTGG
Cuts at: 7802 7802
Size: 10853
BstYI r'GATC_y
Cuts at: 1 778 1167 3875 4604 5077 5337 5787 6426
Size: 777 389 2708 729 473 260 450 639
Cuts at: 6426 6450 7119 7641 7878 8345 8368 8703 8949
Size: 24 669 522 237 467 23 335 246
Cuts at: 8949 9255 9267 9353 10405 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2708 1052 777 729 669 639 522 473
467 450 449 389 335 306 260 246
237 86 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 5104 7815 5104
Size: 2711 8142
BtgI C'CryG_G
Cuts at: 107 125 6233 107
Size: 18 6108 4727
Fragments arranged by size:
6108 4727 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4684 8803 8984 9147 4684
Size: 4119 181 163 6390
Fragments arranged by size:
6390 4119 181 163
BtsI GCAGTG_nn'
Cuts at: 828 6910 8096 828
Size: 6082 1186 3585
Fragments arranged by size:
6082 3585 1186
ClaI AT'CG_AT
Cuts at: 320 320
Size: 10853
DraI TTT'AAA
Cuts at: 3521 5645 6774 7852 3521
Size: 2124 1129 1078 6522
Fragments arranged by size:
6522 2124 1129 1078
DraIII CAC_nnn'GTG
Cuts at: 1618 1618
Size: 10853
DrdI GACnn_nn'nnGTC
Cuts at: 214 8559 9903 214
Size: 8345 1344 1164
Fragments arranged by size:
8345 1344 1164
EaeI y'GGCC_r
Cuts at: 128 148 8438 8612 9003 9030 10375 128
Size: 20 8290 174 391 27 1345 606
Fragments arranged by size:
8290 1345 606 391 174 27 20
EagI C'GGCC_G
Cuts at: 148 8438 148
Size: 8290 2563
EarI CTCTTCn'nnn_
Cuts at: 1323 1359 2330 3470 3866 4052 4454 5712 6052
Size: 36 971 1140 396 186 402 1258 340
Cuts at: 6052 6557 8876 9086 1323
Size: 505 2319 210 3090
Fragments arranged by size:
3090 2319 1258 1140 971 505 402 396
340 210 186 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 9061 9803 9949 10663 127
Size: 8934 742 146 714 317
Fragments arranged by size:
8934 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 2340 7865 2340
Size: 5525 5328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 967 1019 1051 1249 1999 2005 2110 2191
Size: 608 52 32 198 750 6 105 81
Cuts at: 2191 2315 2354 2399 2641 2728 3976 4478 4822
Size: 124 39 45 242 87 1248 502 344
Cuts at: 4822 5242 5761 6283 7054 8678 9110 9463 10125
Size: 420 519 522 771 1624 432 353 662
Cuts at: 10125 359
Size: 1087
Fragments arranged by size:
1624 1248 1087 771 750 662 608 522
519 502 432 420 353 344 242 198
124 105 87 81 52 45 39 32
6
EcoO109I rG'GnC_Cy
Cuts at: 3383 4833 6735 7798 7992 3383
Size: 1450 1902 1063 194 6244
Fragments arranged by size:
6244 1902 1450 1063 194
EcoRI G'AATT_C
Cuts at: 977 987 2485 6130 6238 7554 7857 977
Size: 10 1498 3645 108 1316 303 3973
Fragments arranged by size:
3973 3645 1498 1316 303 108 10
EcoRV GAT'ATC
Cuts at: 7289 7289
Size: 10853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 190 1279 1388 2431 3391 4129 4408 4483 6816
Size: 1089 109 1043 960 738 279 75 2333
Cuts at: 6816 8872 190
Size: 2056 2171
Fragments arranged by size:
2333 2171 2056 1089 1043 960 738 279
109 75
FspI TGC'GCA
Cuts at: 8634 8634
Size: 10853
HaeII r_GCGC'y
Cuts at: 848 8287 8535 9765 10536 848
Size: 7439 248 1230 771 1165
Fragments arranged by size:
7439 1230 1165 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 563 595 896 928 1194 1226 2187 2219 2547
Size: 32 301 32 266 32 961 32 328
Cuts at: 2547 2579 4051 4053 4083 4085 5428 5460 6063
Size: 32 1472 2 30 2 1343 32 603
Cuts at: 6063 6095 6394 6426 6727 6759 7233 7265 7672
Size: 32 299 32 301 32 474 32 407
Cuts at: 7672 7704 8695 8725 8727 8757 563
Size: 32 991 30 2 30 2659
Fragments arranged by size:
2659 1472 1343 991 961 603 474 407
328 301 301 299 266 32 32 32
32 32 32 32 32 32 32 32
30 30 30 2 2 2
HincII GTy'rAC
Cuts at: 971 2901 4754 5042 6878 7264 7892 971
Size: 1930 1853 288 1836 386 628 3932
Fragments arranged by size:
3932 1930 1853 1836 628 386 288
HindIII A'AGCT_T
Cuts at: 888 1462 2243 2850 5610 6595 7132 888
Size: 574 781 607 2760 985 537 4609
Fragments arranged by size:
4609 2760 985 781 607 574 537
HpaI GTT'AAC
Cuts at: 971 2901 6878 971
Size: 1930 3977 4946
Fragments arranged by size:
4946 3977 1930
KasI G'GCGC_C
Cuts at: 8283 8531 8283
Size: 248 10605
KpnI G_GTAC'C
Cuts at: 7873 7873
Size: 10853
MfeI C'AATT_G
Cuts at: 1231 3235 1231
Size: 2004 8849
MluI A'CGCG_T
Cuts at: 10286 10286
Size: 10853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2039 3222 9612 9796 2039
Size: 1183 6390 184 3096
Fragments arranged by size:
6390 3096 1183 184
MscI TGG'CCA
Cuts at: 8614 8614
Size: 10853
MslI CAynn'nnrTG
Cuts at: 272 5315 8842 8969 272
Size: 5043 3527 127 2156
Fragments arranged by size:
5043 3527 2156 127
MspA1I CmG'CkG
Cuts at: 153 2706 4134 4936 7346 7964 8278 8638 9422
Size: 2553 1428 802 2410 618 314 360 784
Cuts at: 9422 9667 153
Size: 245 1339
Fragments arranged by size:
2553 2410 1428 1339 802 784 618 360
314 245
NaeI GCC'GGC
Cuts at: 9034 9034
Size: 10853
NarI GG'CG_CC
Cuts at: 8284 8532 8284
Size: 248 10605
NcoI C'CATG_G
Cuts at: 107 6233 107
Size: 6126 4727
NdeI CA'TA_TG
Cuts at: 2510 3020 2510
Size: 510 10343
NgoMIV G'CCGG_C
Cuts at: 9032 9032
Size: 10853
NotI GC'GGCC_GC
Cuts at: 148 148
Size: 10853
NsiI A_TGCA'T
Cuts at: 5219 5378 6941 5219
Size: 159 1563 9131
Fragments arranged by size:
9131 1563 159
NspI r_CATG'y
Cuts at: 5217 5376 7906 8406 10009 5217
Size: 159 2530 500 1603 6061
Fragments arranged by size:
6061 2530 1603 500 159
PasI CC'CwG_GG
Cuts at: 428 428
Size: 10853
PciI A'CATG_T
Cuts at: 10005 10005
Size: 10853
PflMI CCAn_nnn'nTGG
Cuts at: 1245 6447 6725 1245
Size: 5202 278 5373
Fragments arranged by size:
5373 5202 278
PfoI T'CCnGG_A
Cuts at: 3007 4169 3007
Size: 1162 9691
PmeI GTTT'AAAC
Cuts at: 7852 7852
Size: 10853
PmlI CAC'GTG
Cuts at: 2671 2671
Size: 10853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9299 9331 9299
Size: 32 10821
PpuMI rG'GwC_Cy
Cuts at: 3383 6735 7798 3383
Size: 3352 1063 6438
Fragments arranged by size:
6438 3352 1063
PshAI GACnn'nnGTC
Cuts at: 4936 4936
Size: 10853
PsiI TTA'TAA
Cuts at: 2468 6958 7231 2468
Size: 4490 273 6090
Fragments arranged by size:
6090 4490 273
PspXI vC'TCGA_Gb
Cuts at: 4747 4747
Size: 10853
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3887 3919 7619 7651 3887
Size: 32 3700 32 7089
Fragments arranged by size:
7089 3700 32 32
PstI C_TGCA'G
Cuts at: 852 2786 3126 7900 8585 852
Size: 1934 340 4774 685 3120
Fragments arranged by size:
4774 3120 1934 685 340
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10853
PvuII CAG'CTG
Cuts at: 2706 4134 4936 8278 8638 2706
Size: 1428 802 3342 360 4921
Fragments arranged by size:
4921 3342 1428 802 360
RsrII CG'GwC_CG
Cuts at: 9048 9048
Size: 10853
SacI G_AGCT'C
Cuts at: 2342 7867 2342
Size: 5525 5328
SalI G'TCGA_C
Cuts at: 7890 7890
Size: 10853
SapI GCTCTTCn'nnn_
Cuts at: 2330 8876 9086 2330
Size: 6546 210 4097
Fragments arranged by size:
6546 4097 210
SbfI CC_TGCA'GG
Cuts at: 7900 7900
Size: 10853
ScaI AGT'ACT
Cuts at: 1080 6527 7067 10370 1080
Size: 5447 540 3303 1563
Fragments arranged by size:
5447 3303 1563 540
SfcI C'TryA_G
Cuts at: 33 848 1813 1928 2747 2782 3122 3716 4349
Size: 815 965 115 819 35 340 594 633
Cuts at: 4349 4372 7208 7896 8581 9549 9740 33
Size: 23 2836 688 685 968 191 1146
Fragments arranged by size:
2836 1146 968 965 819 815 688 685
633 594 340 191 115 35 23
SfoI GGC'GCC
Cuts at: 8285 8533 8285
Size: 248 10605
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10853
SmaI CCC'GGG
Cuts at: 7875 7875
Size: 10853
SmlI C'TyrA_G
Cuts at: 862 2542 2816 2918 2941 3577 4747 4901 4924
Size: 1680 274 102 23 636 1170 154 23
Cuts at: 4924 5146 5506 5755 6976 7734 8008 8923 9637
Size: 222 360 249 1221 758 274 915 714
Cuts at: 9637 9899 862
Size: 262 1816
Fragments arranged by size:
1816 1680 1221 1170 915 758 714 636
360 274 274 262 249 222 154 102
23 23
SpeI A'CTAG_T
Cuts at: 67 67
Size: 10853
SphI G_CATG'C
Cuts at: 7906 8406 7906
Size: 500 10353
SspI AAT'ATT
Cuts at: 3261 3261
Size: 10853
StuI AGG'CCT
Cuts at: 3722 7745 3722
Size: 4023 6830
StyI C'CwwG_G
Cuts at: 107 992 1380 3983 4076 4709 6233 6800 7801
Size: 885 388 2603 93 633 1524 567 1001
Cuts at: 7801 7987 8341 107
Size: 186 354 2619
Fragments arranged by size:
2619 2603 1524 1001 885 633 567 388
354 186 93
SwaI ATTT'AAAT
Cuts at: 5645 5645
Size: 10853
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2605 2644 5966 8436 8569 9028 9237 2605
Size: 39 3322 2470 133 459 209 4221
Fragments arranged by size:
4221 3322 2470 459 209 133 39
TatI w'GTAC_w
Cuts at: 1078 1272 3468 4824 6525 7065 7353 10368 1078
Size: 194 2196 1356 1701 540 288 3015 1563
Fragments arranged by size:
3015 2196 1701 1563 1356 540 288 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 965 997 1017 1049 4945 4977 10370 10402 965
Size: 32 20 32 3896 32 5393 32 1416
Fragments arranged by size:
5393 3896 1416 32 32 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 8650 8650
Size: 10853
XbaI T'CTAG_A
Cuts at: 1725 6037 7884 1725
Size: 4312 1847 4694
Fragments arranged by size:
4694 4312 1847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 8348 8348
Size: 10853
XhoI C'TCGA_G
Cuts at: 4747 4747
Size: 10853
XmaI C'CCGG_G
Cuts at: 7873 7873
Size: 10853
XmnI GAAnn'nnTTC
Cuts at: 2384 4040 4391 4687 5421 5495 6646 7587 2384
Size: 1656 351 296 734 74 1151 941 5650
Fragments arranged by size:
5650 1656 1151 941 734 351 296 74
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AleI AloI ApaI AscI
AvrII BcgI BglI BmgBI BmtI BsiWI BsmI BspEI
BssHII BstZ17I CspCI EcoNI FseI FspAI NheI NruI
PacI PspOMI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI ZraI