Restriction map for
pF1KB0056
(Circular) (Six-base) MAPSORT of: pF1KB0056.seq Check: 347 from: 1 to: 7712
pF1KB0056 7712 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1650 1680 2436 3378 4323 4353 1650
Size: 30 756 942 945 30 5009
Fragments arranged by size:
5009 945 942 756 30 30
AccI GT'mk_AC
Cuts at: 4187 4553 4750 4187
Size: 366 197 7149
Fragments arranged by size:
7149 366 197
Acc65I G'GTAC_C
Cuts at: 4728 4728
Size: 7712
AclI AA'CG_TT
Cuts at: 7552 7552
Size: 7712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 1241 1454 1766 2095 4051 4133 5537 5969
Size: 438 213 312 329 1956 82 1404 432
Cuts at: 5969 6716 6984 803
Size: 747 268 1531
Fragments arranged by size:
1956 1531 1404 747 438 432 329 312
268 213 82
AflIII A'CryG_T
Cuts at: 5064 6168 7145 5064
Size: 1104 977 5631
Fragments arranged by size:
5631 1104 977
AgeI A'CCGG_T
Cuts at: 2457 6100 2457
Size: 3643 4069
AleI CACnn'nnGTG
Cuts at: 1813 1813
Size: 7712
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4201 4233 4201
Size: 32 7680
AlwNI CAG_nnn'CTG
Cuts at: 423 438 1201 1432 1630 2073 2673 3417 3475
Size: 15 763 231 198 443 600 744 58
Cuts at: 3475 3604 3729 3939 4083 5017 6584 423
Size: 129 125 210 144 934 1567 1551
Fragments arranged by size:
1567 1551 934 763 744 600 443 231
210 198 144 129 125 58 15
ApaI G_GGCC'C
Cuts at: 692 769 944 970 1095 1363 1381 1509 2764
Size: 77 175 26 125 268 18 128 1255
Cuts at: 2764 3010 4276 4643 692
Size: 246 1266 367 3761
Fragments arranged by size:
3761 1266 1255 367 268 246 175 128
125 77 26 18
ApaLI G'TGCA_C
Cuts at: 6482 6482
Size: 7712
ApoI r'AATT_y
Cuts at: 613 714 4716 7065 7321 613
Size: 101 4002 2349 256 1004
Fragments arranged by size:
4002 2349 1004 256 101
AseI AT'TA_AT
Cuts at: 20 20
Size: 7712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7712
AvaI C'yCGr_G
Cuts at: 120 247 493 605 800 971 2372 2876 3404
Size: 127 246 112 195 171 1401 504 528
Cuts at: 3404 4328 4631 4670 4732 120
Size: 924 303 39 62 3100
Fragments arranged by size:
3100 1401 924 528 504 303 246 195
171 127 112 62 39
AvrII C'CTAG_G
Cuts at: 2770 3355 2770
Size: 585 7127
BamHI G'GATC_C
Cuts at: 2463 4737 2463
Size: 2274 5438
BanI G'GyrC_C
Cuts at: 1602 4728 5142 5390 5425 1602
Size: 3126 414 248 35 3889
Fragments arranged by size:
3889 3126 414 248 35
BanII G_rGCy'C
Cuts at: 692 769 944 970 1095 1363 1381 1509 2119
Size: 77 175 26 125 268 18 128 610
Cuts at: 2119 2398 2764 2795 2995 3010 3137 3710 4158
Size: 279 366 31 200 15 127 573 448
Cuts at: 4158 4276 4643 4726 5756 692
Size: 118 367 83 1030 2648
Fragments arranged by size:
2648 1030 610 573 448 367 366 279
268 200 175 128 127 125 118 83
77 31 26 18 15
BbeI G_GCGC'C
Cuts at: 5146 5394 5146
Size: 248 7464
BbsI GAAGACnn'nnnn_
Cuts at: 2484 2613 4527 2484
Size: 129 1914 5669
Fragments arranged by size:
5669 1914 129
BbvCI CC'TCA_GC
Cuts at: 1172 3900 1172
Size: 2728 4984
BciVI GTATCCnnnnn_n'
Cuts at: 881 3743 4589 5610 6371 7712 881
Size: 2862 846 1021 761 1341 881
Fragments arranged by size:
2862 1341 1021 881 846 761
BclI T'GATC_A
Cuts at: 1053 5232 1053
Size: 4179 3533
BglI GCCn_nnn'nGGC
Cuts at: 974 3434 4136 974
Size: 2460 702 4550
Fragments arranged by size:
4550 2460 702
BglII A'GATC_T
Cuts at: 3776 5227 7264 3776
Size: 1451 2037 4224
Fragments arranged by size:
4224 2037 1451
BlpI GC'TnA_GC
Cuts at: 416 3662 4824 416
Size: 3246 1162 3304
Fragments arranged by size:
3304 3246 1162
Bme1580I G_kGCm'C
Cuts at: 537 692 769 944 970 1095 1363 1381 1509
Size: 155 77 175 26 125 268 18 128
Cuts at: 1509 1605 2141 2452 2764 3010 3695 4276 4643
Size: 96 536 311 312 246 685 581 367
Cuts at: 4643 5337 5430 6486 537
Size: 694 93 1056 1763
Fragments arranged by size:
1763 1056 694 685 581 536 367 312
311 268 246 175 155 128 125 96
93 77 26 18
BmrI ACTGGGnnnn_n'
Cuts at: 825 1383 1864 1936 2128 3505 3638 3639 4034
Size: 558 481 72 192 1377 133 1 395
Cuts at: 4034 4924 5005 5340 7484 825
Size: 890 81 335 2144 1053
Fragments arranged by size:
2144 1377 1053 890 558 481 395 335
192 133 81 72 1
BmtI G_CTAG'C
Cuts at: 4367 4367
Size: 7712
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2597 3729 4012 6079 2597
Size: 1132 283 2067 4230
Fragments arranged by size:
4230 2067 1132 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 781 1286 1685 1697 1772 2296 2570 3317
Size: 515 505 399 12 75 524 274 747
Cuts at: 3317 4007 266
Size: 690 3971
Fragments arranged by size:
3971 747 690 524 515 505 399 274
75 12
Bpu10I CC'TnA_GC
Cuts at: 978 1172 3900 4601 978
Size: 194 2728 701 4089
Fragments arranged by size:
4089 2728 701 194
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1561 2382 3247 3910 4489 4888 5767 6259
Size: 453 821 865 663 579 399 879 492
Cuts at: 6259 6557 1108
Size: 298 2263
Fragments arranged by size:
2263 879 865 821 663 579 492 453
399 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 7416 3354
Size: 4062 3650
BsaAI yAC'GTr
Cuts at: 182 5065 182
Size: 4883 2829
BsaBI GATnn'nnATC
Cuts at: 6 5226 7263 6
Size: 5220 2037 455
Fragments arranged by size:
5220 2037 455
BsaHI Gr'CG_yC
Cuts at: 454 5143 5391 6093 7589 454
Size: 4689 248 702 1496 577
Fragments arranged by size:
4689 1496 702 577 248
BsaWI w'CCGG_w
Cuts at: 2457 4901 5422 6100 6374 6521 6932 2457
Size: 2444 521 678 274 147 411 3237
Fragments arranged by size:
3237 2444 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 686 716 1647 1677 1770 1800 4201 4231 4651
Size: 30 931 30 93 30 2401 30 420
Cuts at: 4651 4675 4681 4705 686
Size: 24 6 24 3693
Fragments arranged by size:
3693 2401 931 420 93 30 30 30
30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1177 1543 1916 2030 2299 2573 2586 2851 2864
Size: 366 373 114 269 274 13 265 13
Cuts at: 2864 2960 3294 3705 4004 4103 4672 1177
Size: 96 334 411 299 99 569 4217
Fragments arranged by size:
4217 569 411 373 366 334 299 274
269 265 114 99 96 13 13
BseYI C'CCAG_C
Cuts at: 483 943 1425 1659 2605 2678 3109 3709 3757
Size: 460 482 234 946 73 431 600 48
Cuts at: 3757 3888 3969 4163 4291 4544 5137 5895 6472
Size: 131 81 194 128 253 593 758 577
Cuts at: 6472 6968 483
Size: 496 1227
Fragments arranged by size:
1227 946 758 600 593 577 496 482
460 431 253 234 194 131 128 81
73 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1679 2693 3832 1679
Size: 1014 1139 5559
Fragments arranged by size:
5559 1139 1014
BsiEI CG_ry'CG
Cuts at: 104 117 5254 5300 6508 104
Size: 13 5137 46 1208 1308
Fragments arranged by size:
5137 1308 1208 46 13
BsiHKAI G_wGCw'C
Cuts at: 561 2229 4726 5504 5694 6486 561
Size: 1668 2497 778 190 792 1787
Fragments arranged by size:
2497 1787 1668 792 778 190
BsmI GAATG_Cn'
Cuts at: 3321 3321
Size: 7712
BsmBI CGTCTCn'nnnn_
Cuts at: 4507 4507
Size: 7712
Bsp1286I G_dGCh'C
Cuts at: 537 561 692 769 944 970 1095 1363 1381
Size: 24 131 77 175 26 125 268 18
Cuts at: 1381 1509 1605 2119 2141 2229 2398 2452 2764
Size: 128 96 514 22 88 169 54 312
Cuts at: 2764 2795 2995 3010 3137 3695 3710 4158 4276
Size: 31 200 15 127 558 15 448 118
Cuts at: 4276 4643 4726 5337 5430 5504 5694 5756 6486
Size: 367 83 611 93 74 190 62 730
Cuts at: 6486 537
Size: 1763
Fragments arranged by size:
1763 730 611 558 514 448 367 312
268 200 190 175 169 131 128 127
125 118 96 93 88 83 77 74
62 54 31 26 24 22 18 15
15
BspEI T'CCGG_A
Cuts at: 6932 6932
Size: 7712
BspHI T'CATG_A
Cuts at: 792 6888 792
Size: 6096 1616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1650 1680 2436 3378 4323 4353 4762 4976 5278
Size: 30 756 942 945 30 409 214 302
Cuts at: 5278 5659 1650
Size: 381 3703
Fragments arranged by size:
3703 945 942 756 409 381 302 214
30 30
BsrBI CCG'CTC
Cuts at: 50 415 1503 1533 2922 4614 6004 6058 7542
Size: 365 1088 30 1389 1692 1390 54 1484
Cuts at: 7542 7707 50
Size: 165 55
Fragments arranged by size:
1692 1484 1390 1389 1088 365 165 55
54 30
BsrDI GCAATG_nn'
Cuts at: 260 327 5624 260
Size: 67 5297 2348
Fragments arranged by size:
5297 2348 67
BsrFI r'CCGG_y
Cuts at: 441 2234 2457 3104 3981 5710 5891 6100 441
Size: 1793 223 647 877 1729 181 209 2053
Fragments arranged by size:
2053 1793 1729 877 647 223 209 181
BssSI C'ACGA_G
Cuts at: 3623 5983 6341 3623
Size: 2360 358 4994
Fragments arranged by size:
4994 2360 358
BstAPI GCAn_nnn'nTGC
Cuts at: 438 1564 1630 3417 5089 438
Size: 1126 66 1787 1672 3061
Fragments arranged by size:
3061 1787 1672 1126 66
BstBI TT'CG_AA
Cuts at: 1859 6073 1859
Size: 4214 3498
BstXI CCAn_nnnn'nTGG
Cuts at: 1872 3117 3502 3681 1872
Size: 1245 385 179 5903
Fragments arranged by size:
5903 1245 385 179
BstYI r'GATC_y
Cuts at: 1 203 2463 3776 4080 4737 5204 5227 5562
Size: 202 2260 1313 304 657 467 23 335
Cuts at: 5562 5808 6820 6906 6918 7264 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2260 1313 1012 657 467 449 346 335
304 246 202 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2756 3002 3017 3717 3737 2756
Size: 246 15 700 20 6731
Fragments arranged by size:
6731 700 246 20 15
BtgI C'CryG_G
Cuts at: 231 552 846 3116 3791 4181 231
Size: 321 294 2270 675 390 3762
Fragments arranged by size:
3762 2270 675 390 321 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1148 5662 5843 6006 1148
Size: 4514 181 163 2854
Fragments arranged by size:
4514 2854 181 163
BtsI GCAGTG_nn'
Cuts at: 436 1502 1854 3759 3844 3904 4955 436
Size: 1066 352 1905 85 60 1051 3193
Fragments arranged by size:
3193 1905 1066 1051 352 85 60
DraI TTT'AAA
Cuts at: 4711 4711
Size: 7712
DrdI GACnn_nn'nnGTC
Cuts at: 331 5418 6276 331
Size: 5087 858 1767
Fragments arranged by size:
5087 1767 858
EaeI y'GGCC_r
Cuts at: 3487 4128 5297 5471 5862 5889 7234 3487
Size: 641 1169 174 391 27 1345 3965
Fragments arranged by size:
3965 1345 1169 641 391 174 27
EagI C'GGCC_G
Cuts at: 5297 5297
Size: 7712
EarI CTCTTCn'nnn_
Cuts at: 1094 1520 1541 1742 1910 5735 5945 1094
Size: 426 21 201 168 3825 210 2861
Fragments arranged by size:
3825 2861 426 210 201 168 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 694 884 2614 3817 5920 6230 6376 7522 694
Size: 190 1730 1203 2103 310 146 1146 884
Fragments arranged by size:
2103 1730 1203 1146 884 310 190 146
EcoICRI GAG'CTC
Cuts at: 4724 4724
Size: 7712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 781 803 1241 1286 1454 1685 1697 1766
Size: 515 22 438 45 168 231 12 69
Cuts at: 1766 1772 2095 2296 2570 3317 4007 4051 4133
Size: 6 323 201 274 747 690 44 82
Cuts at: 4133 5537 5969 6716 6984 266
Size: 1404 432 747 268 994
Fragments arranged by size:
1404 994 747 747 690 515 438 432
323 274 268 231 201 168 82 69
45 44 22 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1787 2769 3254 4634 1787
Size: 982 485 1380 4865
Fragments arranged by size:
4865 1380 982 485
EcoO109I rG'GnC_Cy
Cuts at: 765 913 967 1091 1318 1359 1360 1377 1378
Size: 148 54 124 227 41 1 17 1
Cuts at: 1378 1408 1761 1900 2211 2760 2761 2774 3006
Size: 30 353 139 311 549 1 13 232
Cuts at: 3006 3013 3048 3226 3280 4273 4352 4444 4478
Size: 7 35 178 54 993 79 92 34
Cuts at: 4478 4639 4640 4667 4851 765
Size: 161 1 27 184 3626
Fragments arranged by size:
3626 993 549 353 311 232 227 184
178 161 148 139 124 92 79 54
54 41 35 34 30 27 17 13
7 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4716 4716
Size: 7712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5731 5731
Size: 7712
FseI GG_CCGG'CC
Cuts at: 2238 2238
Size: 7712
FspI TGC'GCA
Cuts at: 5493 5493
Size: 7712
HaeII r_GCGC'y
Cuts at: 1554 3729 5146 5394 6416 7395 1554
Size: 2175 1417 248 1022 979 1871
Fragments arranged by size:
2175 1871 1417 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 686 718 1177 1209 2271 2303 3523 3555 3655
Size: 32 459 32 1062 32 1220 32 100
Cuts at: 3655 3687 4072 4104 4651 4683 5554 5584 5586
Size: 32 385 32 547 32 871 30 2
Cuts at: 5586 5616 686
Size: 30 2782
Fragments arranged by size:
2782 1220 1062 871 547 459 385 100
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1107 2805 4554 4751 1107
Size: 1698 1749 197 4068
Fragments arranged by size:
4068 1749 1698 197
KasI G'GCGC_C
Cuts at: 5142 5390 5142
Size: 248 7464
KpnI G_GTAC'C
Cuts at: 4732 4732
Size: 7712
MluI A'CGCG_T
Cuts at: 7145 7145
Size: 7712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 410 488 643 1040 2688 6383 6567 410
Size: 78 155 397 1648 3695 184 1555
Fragments arranged by size:
3695 1648 1555 397 184 155 78
MscI TGG'CCA
Cuts at: 4130 5473 4130
Size: 1343 6369
MslI CAynn'nnrTG
Cuts at: 566 791 1813 3169 5701 5828 566
Size: 225 1022 1356 2532 127 2450
Fragments arranged by size:
2532 2450 1356 1022 225 127
MspA1I CmG'CkG
Cuts at: 217 423 848 2052 2509 2810 4197 4823 5137
Size: 206 425 1204 457 301 1387 626 314
Cuts at: 5137 5497 6510 6755 217
Size: 360 1013 245 1174
Fragments arranged by size:
1387 1204 1174 1013 626 457 425 360
314 301 245 206
NaeI GCC'GGC
Cuts at: 443 2236 3983 5893 443
Size: 1793 1747 1910 2262
Fragments arranged by size:
2262 1910 1793 1747
NarI GG'CG_CC
Cuts at: 5143 5391 5143
Size: 248 7464
NcoI C'CATG_G
Cuts at: 231 552 3116 3791 4181 231
Size: 321 2564 675 390 3762
Fragments arranged by size:
3762 2564 675 390 321
NgoMIV G'CCGG_C
Cuts at: 441 2234 3981 5891 441
Size: 1793 1747 1910 2262
Fragments arranged by size:
2262 1910 1793 1747
NheI G'CTAG_C
Cuts at: 4363 4363
Size: 7712
NspI r_CATG'y
Cuts at: 1542 4765 5265 6172 1542
Size: 3223 500 907 3082
Fragments arranged by size:
3223 3082 907 500
PasI CC'CwG_GG
Cuts at: 909 1384 2166 3832 909
Size: 475 782 1666 4789
Fragments arranged by size:
4789 1666 782 475
PciI A'CATG_T
Cuts at: 6168 6168
Size: 7712
PflMI CCAn_nnn'nTGG
Cuts at: 1402 3817 3939 1402
Size: 2415 122 5175
Fragments arranged by size:
5175 2415 122
PfoI T'CCnGG_A
Cuts at: 3701 4409 3701
Size: 708 7004
PmeI GTTT'AAAC
Cuts at: 4711 4711
Size: 7712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4201 4233 6851 6883 4201
Size: 32 2618 32 5030
Fragments arranged by size:
5030 2618 32 32
PpuMI rG'GwC_Cy
Cuts at: 1318 1408 1900 3226 3280 4444 4478 4667 1318
Size: 90 492 1326 54 1164 34 189 4363
Fragments arranged by size:
4363 1326 1164 492 189 90 54 34
PspOMI G'GGCC_C
Cuts at: 688 765 940 966 1091 1359 1377 1505 2760
Size: 77 175 26 125 268 18 128 1255
Cuts at: 2760 3006 4272 4639 688
Size: 246 1266 367 3761
Fragments arranged by size:
3761 1266 1255 367 268 246 175 128
125 77 26 18
PstI C_TGCA'G
Cuts at: 242 629 1677 3235 4121 4286 4759 5444 242
Size: 387 1048 1558 886 165 473 685 2510
Fragments arranged by size:
2510 1558 1048 886 685 473 387 165
PvuI CG_AT'CG
Cuts at: 104 117 104
Size: 13 7699
PvuII CAG'CTG
Cuts at: 4197 5137 5497 4197
Size: 940 360 6412
Fragments arranged by size:
6412 940 360
RsrII CG'GwC_CG
Cuts at: 5907 5907
Size: 7712
SacI G_AGCT'C
Cuts at: 4726 4726
Size: 7712
SacII CC_GC'GG
Cuts at: 849 849
Size: 7712
SalI G'TCGA_C
Cuts at: 4552 4749 4552
Size: 197 7515
SanDI GG'GwC_CC
Cuts at: 3280 4667 3280
Size: 1387 6325
SapI GCTCTTCn'nnn_
Cuts at: 1541 1742 5735 5945 1541
Size: 201 3993 210 3308
Fragments arranged by size:
3993 3308 210 201
SbfI CC_TGCA'GG
Cuts at: 629 4759 629
Size: 4130 3582
ScaI AGT'ACT
Cuts at: 7229 7229
Size: 7712
SexAI A'CCwGG_T
Cuts at: 3501 3501
Size: 7712
SfcI C'TryA_G
Cuts at: 33 238 625 811 1673 2947 3231 3962 4117
Size: 205 387 186 862 1274 284 731 155
Cuts at: 4117 4282 4524 4755 5440 6433 6624 33
Size: 165 242 231 685 993 191 1121
Fragments arranged by size:
1274 1121 993 862 731 685 387 284
242 231 205 191 186 165 155
SfoI GGC'GCC
Cuts at: 5144 5392 5144
Size: 248 7464
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7712
SgrAI Cr'CCGG_yG
Cuts at: 2457 2457
Size: 7712
SmaI CCC'GGG
Cuts at: 802 3406 4734 802
Size: 2604 1328 3780
Fragments arranged by size:
3780 2604 1328
SmlI C'TyrA_G
Cuts at: 605 1123 1576 2397 3262 3925 4504 4631 4867
Size: 518 453 821 865 663 579 127 236
Cuts at: 4867 5782 6274 6536 605
Size: 915 492 262 1781
Fragments arranged by size:
1781 915 865 821 663 579 518 492
453 262 236 127
SnaBI TAC'GTA
Cuts at: 182 182
Size: 7712
SpeI A'CTAG_T
Cuts at: 67 339 3526 67
Size: 272 3187 4253
Fragments arranged by size:
4253 3187 272
SphI G_CATG'C
Cuts at: 1542 4765 5265 1542
Size: 3223 500 3989
Fragments arranged by size:
3989 3223 500
StuI AGG'CCT
Cuts at: 3294 4570 3294
Size: 1276 6436
StyI C'CwwG_G
Cuts at: 134 231 552 656 1961 2207 2725 2770 3116
Size: 97 321 104 1305 246 518 45 346
Cuts at: 3116 3124 3355 3791 4181 4437 4606 4846 5200
Size: 8 231 436 390 256 169 240 354
Cuts at: 5200 134
Size: 2646
Fragments arranged by size:
2646 1305 518 436 390 354 346 321
256 246 240 231 169 104 97 45
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 2259 2854 3476 4558 5295 5428 5887 6096
Size: 1694 595 622 1082 737 133 459 209
Cuts at: 6096 565
Size: 2181
Fragments arranged by size:
2181 1694 1082 737 622 595 459 209
133
TatI w'GTAC_w
Cuts at: 7227 7227
Size: 7712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 818 850 1171 1203 1661 1693 3998 4030 7229
Size: 32 321 32 458 32 2305 32 3199
Cuts at: 7229 7261 818
Size: 32 1269
Fragments arranged by size:
3199 2305 1269 458 321 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3503 5509 3503
Size: 2006 5706
XbaI T'CTAG_A
Cuts at: 2328 4743 2328
Size: 2415 5297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 559 1198 2474 5207 559
Size: 639 1276 2733 3064
Fragments arranged by size:
3064 2733 1276 639
XhoI C'TCGA_G
Cuts at: 605 4631 605
Size: 4026 3686
XmaI C'CCGG_G
Cuts at: 800 3404 4732 800
Size: 2604 1328 3780
Fragments arranged by size:
3780 2604 1328
XmnI GAAnn'nnTTC
Cuts at: 1919 1919
Size: 7712
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI BaeI BcgI
BfrBI BmgBI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SfiI SrfI SspI SwaI ZraI