Restriction map for
pF1KB0053
(Circular) (Six-base) MAPSORT of: pF1KB0053.seq Check: 1198 from: 1 to: 7049
pF1KB0053 7049 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1202 4087 1202
Size: 2885 4164
Acc65I G'GTAC_C
Cuts at: 4065 4065
Size: 7049
AclI AA'CG_TT
Cuts at: 6889 6889
Size: 7049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1030 2270 2675 3047 3377 3884 4874 5306
Size: 269 1240 405 372 330 507 990 432
Cuts at: 5306 6053 6321 761
Size: 747 268 1489
Fragments arranged by size:
1489 1240 990 747 507 432 405 372
330 269 268
AflIII A'CryG_T
Cuts at: 4401 5505 6482 4401
Size: 1104 977 4968
Fragments arranged by size:
4968 1104 977
AgeI A'CCGG_T
Cuts at: 5437 5437
Size: 7049
AhdI GACnn_n'nnGTC
Cuts at: 3351 3351
Size: 7049
AleI CACnn'nnGTG
Cuts at: 151 151
Size: 7049
AlwNI CAG_nnn'CTG
Cuts at: 2947 3208 3973 4354 5921 2947
Size: 261 765 381 1567 4075
Fragments arranged by size:
4075 1567 765 381 261
ApaI G_GGCC'C
Cuts at: 283 283
Size: 7049
ApaLI G'TGCA_C
Cuts at: 5819 5819
Size: 7049
ApoI r'AATT_y
Cuts at: 769 868 1060 1116 1230 1315 1527 3136 4053
Size: 99 192 56 114 85 212 1609 917
Cuts at: 4053 6402 6658 769
Size: 2349 256 1160
Fragments arranged by size:
2349 1609 1160 917 256 212 192 114
99 85 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 7049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7049
AvaI C'yCGr_G
Cuts at: 4069 4069
Size: 7049
BamHI G'GATC_C
Cuts at: 3708 4074 3708
Size: 366 6683
BanI G'GyrC_C
Cuts at: 4065 4479 4727 4762 4065
Size: 414 248 35 6352
Fragments arranged by size:
6352 414 248 35
BanII G_rGCy'C
Cuts at: 283 3341 3400 4063 5093 283
Size: 3058 59 663 1030 2239
Fragments arranged by size:
3058 2239 1030 663 59
BbeI G_GCGC'C
Cuts at: 4483 4731 4483
Size: 248 6801
BbsI GAAGACnn'nnnn_
Cuts at: 409 560 910 1598 2258 3227 409
Size: 151 350 688 660 969 4231
Fragments arranged by size:
4231 969 688 660 350 151
BbvCI CC'TCA_GC
Cuts at: 1667 1667
Size: 7049
BciVI GTATCCnnnnn_n'
Cuts at: 1237 2154 4947 5708 7049 1237
Size: 917 2793 761 1341 1237
Fragments arranged by size:
2793 1341 1237 917 761
BclI T'GATC_A
Cuts at: 1557 2322 4569 1557
Size: 765 2247 4037
Fragments arranged by size:
4037 2247 765
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 7049
BglII A'GATC_T
Cuts at: 541 1209 4564 6601 541
Size: 668 3355 2037 989
Fragments arranged by size:
3355 2037 989 668
BlpI GC'TnA_GC
Cuts at: 4161 4161
Size: 7049
Bme1580I G_kGCm'C
Cuts at: 148 283 4674 4767 5823 148
Size: 135 4391 93 1056 1374
Fragments arranged by size:
4391 1374 1056 135 93
BmrI ACTGGGnnnn_n'
Cuts at: 3041 3722 4261 4342 4677 6821 3041
Size: 681 539 81 335 2144 3269
Fragments arranged by size:
3269 2144 681 539 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2297 5416 2297
Size: 3119 3930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 564 1709 2426 3056 3334 3662 491
Size: 73 1145 717 630 278 328 3878
Fragments arranged by size:
3878 1145 717 630 328 278 73
Bpu10I CC'TnA_GC
Cuts at: 491 1667 3473 491
Size: 1176 1806 4067
Fragments arranged by size:
4067 1806 1176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 3384 3703 4225 5104 5596 5894 2267
Size: 1117 319 522 879 492 298 3422
Fragments arranged by size:
3422 1117 879 522 492 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 6753 6753
Size: 7049
BsaAI yAC'GTr
Cuts at: 4402 4402
Size: 7049
BsaBI GATnn'nnATC
Cuts at: 6 3707 3713 4563 6600 6
Size: 3701 6 850 2037 455
Fragments arranged by size:
3701 2037 850 455 6
BsaHI Gr'CG_yC
Cuts at: 4480 4728 5430 6926 4480
Size: 248 702 1496 4603
Fragments arranged by size:
4603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4238 4759 5437 5711 5858 6269 4238
Size: 521 678 274 147 411 5018
Fragments arranged by size:
5018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 1792 1822 3642 3660 3672 3690 3879
Size: 30 1475 30 1820 18 12 18 189
Cuts at: 3879 3909 287
Size: 30 3427
Fragments arranged by size:
3427 1820 1475 189 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 185 967 970 1303 1447 1450 1753 1756 1759
Size: 782 3 333 144 3 303 3 3
Cuts at: 1759 1762 1813 3004 3019 3046 3494 3900 3930
Size: 3 51 1191 15 27 448 406 30
Cuts at: 3930 185
Size: 3304
Fragments arranged by size:
3304 1191 782 448 406 333 303 144
51 30 27 15 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 3331 4474 5232 5809 6305 3331
Size: 1143 758 577 496 4075
Fragments arranged by size:
4075 1143 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 803 4059 803
Size: 3256 3793
BsiEI CG_ry'CG
Cuts at: 104 4591 4637 5845 104
Size: 4487 46 1208 1308
Fragments arranged by size:
4487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2283 3341 3400 4063 4841 5031 5823 2283
Size: 1058 59 663 778 190 792 3509
Fragments arranged by size:
3509 1058 792 778 663 190 59
BsmBI CGTCTCn'nnnn_
Cuts at: 405 405
Size: 7049
Bsp1286I G_dGCh'C
Cuts at: 148 283 2283 3341 3400 4063 4674 4767 4841
Size: 135 2000 1058 59 663 611 93 74
Cuts at: 4841 5031 5093 5823 148
Size: 190 62 730 1374
Fragments arranged by size:
2000 1374 1058 730 663 611 190 135
93 74 62 59
BspEI T'CCGG_A
Cuts at: 6269 6269
Size: 7049
BspHI T'CATG_A
Cuts at: 6225 6225
Size: 7049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1995 3367 4099 4313 4615 4996 1995
Size: 1372 732 214 302 381 4048
Fragments arranged by size:
4048 1372 732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5341 5395 6879 7044 50
Size: 5291 54 1484 165 55
Fragments arranged by size:
5291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1327 1360 4961 1327
Size: 33 3601 3415
Fragments arranged by size:
3601 3415 33
BsrFI r'CCGG_y
Cuts at: 3224 5047 5228 5437 3224
Size: 1823 181 209 4836
Fragments arranged by size:
4836 1823 209 181
BsrGI T'GTAC_A
Cuts at: 1055 1146 3445 1055
Size: 91 2299 4659
Fragments arranged by size:
4659 2299 91
BssSI C'ACGA_G
Cuts at: 5320 5678 5320
Size: 358 6691
BstAPI GCAn_nnn'nTGC
Cuts at: 4426 4426
Size: 7049
BstBI TT'CG_AA
Cuts at: 3282 5410 3282
Size: 2128 4921
BstXI CCAn_nnnn'nTGG
Cuts at: 1213 1213
Size: 7049
BstYI r'GATC_y
Cuts at: 1 541 1209 2154 3708 4074 4541 4564 4899
Size: 540 668 945 1554 366 467 23 335
Cuts at: 4899 5145 6157 6243 6255 6601 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1554 1012 945 668 540 467 449 366
346 335 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4999 5180 5343 4999
Size: 181 163 6705
Fragments arranged by size:
6705 181 163
BtsI GCAGTG_nn'
Cuts at: 815 939 999 3010 4292 815
Size: 124 60 2011 1282 3572
Fragments arranged by size:
3572 2011 1282 124 60
ClaI AT'CG_AT
Cuts at: 3735 3735
Size: 7049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3299 3334 3299
Size: 35 7014
DraI TTT'AAA
Cuts at: 842 2834 3899 4048 842
Size: 1992 1065 149 3843
Fragments arranged by size:
3843 1992 1065 149
DraIII CAC_nnn'GTG
Cuts at: 3049 3049
Size: 7049
DrdI GACnn_nn'nnGTC
Cuts at: 4755 5613 4755
Size: 858 6191
EaeI y'GGCC_r
Cuts at: 4634 4808 5199 5226 6571 4634
Size: 174 391 27 1345 5112
Fragments arranged by size:
5112 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4634 4634
Size: 7049
EarI CTCTTCn'nnn_
Cuts at: 557 944 1730 1757 1790 2594 2630 2694 3023
Size: 387 786 27 33 804 36 64 329
Cuts at: 3023 3269 3948 5072 5282 557
Size: 246 679 1124 210 2324
Fragments arranged by size:
2324 1124 804 786 679 387 329 246
210 64 36 33 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5257 5567 5713 6859 5257
Size: 310 146 1146 5447
Fragments arranged by size:
5447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3339 3398 4061 3339
Size: 59 663 6327
Fragments arranged by size:
6327 663 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 564 761 1030 1709 2270 2426 2675 3047
Size: 73 197 269 679 561 156 249 372
Cuts at: 3047 3056 3334 3377 3662 3884 4874 5306 6053
Size: 9 278 43 285 222 990 432 747
Cuts at: 6053 6321 491
Size: 268 1219
Fragments arranged by size:
1219 990 747 679 561 432 372 285
278 269 268 249 222 197 156 73
43 9
EcoO109I rG'GnC_Cy
Cuts at: 114 279 1911 4188 114
Size: 165 1632 2277 2975
Fragments arranged by size:
2975 2277 1632 165
EcoRI G'AATT_C
Cuts at: 4053 4053
Size: 7049
EcoRV GAT'ATC
Cuts at: 1111 1111
Size: 7049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 867 2677 3841 5068 867
Size: 1810 1164 1227 2848
Fragments arranged by size:
2848 1810 1227 1164
FspI TGC'GCA
Cuts at: 4830 4830
Size: 7049
HaeII r_GCGC'y
Cuts at: 2100 4483 4731 5753 6732 2100
Size: 2383 248 1022 979 2417
Fragments arranged by size:
2417 2383 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 550 556 582 588 2001 2033 2875 2907 3337
Size: 6 26 6 1413 32 842 32 430
Cuts at: 3337 3369 3660 3692 4891 4921 4923 4953 550
Size: 32 291 32 1199 30 2 30 2646
Fragments arranged by size:
2646 1413 1199 842 430 291 32 32
32 32 30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 1347 1575 3983 4088 1347
Size: 228 2408 105 4308
Fragments arranged by size:
4308 2408 228 105
HindIII A'AGCT_T
Cuts at: 2231 2231
Size: 7049
HpaI GTT'AAC
Cuts at: 1347 1575 3983 1347
Size: 228 2408 4413
Fragments arranged by size:
4413 2408 228
KasI G'GCGC_C
Cuts at: 4479 4727 4479
Size: 248 6801
KpnI G_GTAC'C
Cuts at: 4069 4069
Size: 7049
MfeI C'AATT_G
Cuts at: 1051 3311 1051
Size: 2260 4789
MluI A'CGCG_T
Cuts at: 6482 6482
Size: 7049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3752 5720 5904 3752
Size: 1968 184 4897
Fragments arranged by size:
4897 1968 184
MscI TGG'CCA
Cuts at: 4810 4810
Size: 7049
MslI CAynn'nnrTG
Cuts at: 151 3634 5038 5165 151
Size: 3483 1404 127 2035
Fragments arranged by size:
3483 2035 1404 127
MspA1I CmG'CkG
Cuts at: 1237 3586 3970 4160 4474 4834 5847 6092 1237
Size: 2349 384 190 314 360 1013 245 2194
Fragments arranged by size:
2349 2194 1013 384 360 314 245 190
NaeI GCC'GGC
Cuts at: 3226 5230 3226
Size: 2004 5045
NarI GG'CG_CC
Cuts at: 4480 4728 4480
Size: 248 6801
NgoMIV G'CCGG_C
Cuts at: 3224 5228 3224
Size: 2004 5045
NspI r_CATG'y
Cuts at: 4102 4602 5509 4102
Size: 500 907 5642
Fragments arranged by size:
5642 907 500
PciI A'CATG_T
Cuts at: 5505 5505
Size: 7049
PflMI CCAn_nnn'nTGG
Cuts at: 3148 3148
Size: 7049
PfoI T'CCnGG_A
Cuts at: 3678 3678
Size: 7049
PmeI GTTT'AAAC
Cuts at: 4048 4048
Size: 7049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6188 6220 6188
Size: 32 7017
PpuMI rG'GwC_Cy
Cuts at: 1911 1911
Size: 7049
PsiI TTA'TAA
Cuts at: 1265 1265
Size: 7049
PspOMI G'GGCC_C
Cuts at: 279 279
Size: 7049
PstI C_TGCA'G
Cuts at: 1242 2964 3017 4096 4781 1242
Size: 1722 53 1079 685 3510
Fragments arranged by size:
3510 1722 1079 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7049
PvuII CAG'CTG
Cuts at: 1237 3586 3970 4474 4834 1237
Size: 2349 384 504 360 3452
Fragments arranged by size:
3452 2349 504 384 360
RsrII CG'GwC_CG
Cuts at: 5244 5244
Size: 7049
SacI G_AGCT'C
Cuts at: 3341 3400 4063 3341
Size: 59 663 6327
Fragments arranged by size:
6327 663 59
SalI G'TCGA_C
Cuts at: 4086 4086
Size: 7049
SapI GCTCTTCn'nnn_
Cuts at: 2694 5072 5282 2694
Size: 2378 210 4461
Fragments arranged by size:
4461 2378 210
SbfI CC_TGCA'GG
Cuts at: 4096 4096
Size: 7049
ScaI AGT'ACT
Cuts at: 1294 6566 1294
Size: 5272 1777
SfcI C'TryA_G
Cuts at: 33 377 1238 1296 2960 3013 4092 4777 5770
Size: 344 861 58 1664 53 1079 685 993
Cuts at: 5770 5961 33
Size: 191 1121
Fragments arranged by size:
1664 1121 1079 993 861 685 344 191
58 53
SfoI GGC'GCC
Cuts at: 4481 4729 4481
Size: 248 6801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7049
SmaI CCC'GGG
Cuts at: 4071 4071
Size: 7049
SmlI C'TyrA_G
Cuts at: 2282 3399 3718 4204 5119 5611 5873 2282
Size: 1117 319 486 915 492 262 3458
Fragments arranged by size:
3458 1117 915 492 486 319 262
SpeI A'CTAG_T
Cuts at: 67 808 67
Size: 741 6308
SphI G_CATG'C
Cuts at: 4102 4602 4102
Size: 500 6549
SspI AAT'ATT
Cuts at: 741 1173 1500 741
Size: 432 327 6290
Fragments arranged by size:
6290 432 327
StyI C'CwwG_G
Cuts at: 4183 4537 4183
Size: 354 6695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3060 4632 4765 5224 5433 3060
Size: 1572 133 459 209 4676
Fragments arranged by size:
4676 1572 459 209 133
TatI w'GTAC_w
Cuts at: 1055 1101 1146 1292 3445 6564 1055
Size: 46 45 146 2153 3119 1540
Fragments arranged by size:
3119 2153 1540 146 46 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 1792 1824 1928 1960 6566 6598 287
Size: 32 1473 32 104 32 4606 32 738
Fragments arranged by size:
4606 1473 738 104 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3672 4846 3672
Size: 1174 5875
XbaI T'CTAG_A
Cuts at: 4080 4080
Size: 7049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1840 4544 1840
Size: 2704 4345
XmaI C'CCGG_G
Cuts at: 4069 4069
Size: 7049
XmnI GAAnn'nnTTC
Cuts at: 1019 2852 3278 3824 1019
Size: 1833 426 546 4244
Fragments arranged by size:
4244 1833 546 426
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BssHII
BstEII BstZ17I Bsu36I BtgI EcoNI FseI FspAI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI