Restriction map for pF1KB0053
 (Circular) (Six-base) MAPSORT of: pF1KB0053.seq  Check: 1198  from: 1  to: 7049



pF1KB0053  7049 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AccI GT'mk_AC

Cuts at:   1202    4087    1202 
   Size:       2885    4164
 
Acc65I G'GTAC_C

Cuts at:   4065    4065 
   Size:       7049
 
AclI AA'CG_TT

Cuts at:   6889    6889 
   Size:       7049
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    761    1030    2270    2675    3047    3377    3884    4874    5306 
   Size:        269    1240     405     372     330     507     990     432

Cuts at:   5306    6053    6321     761 
   Size:        747     268    1489

  Fragments arranged by size: 

                1489    1240     990     747     507     432     405     372
                 330     269     268
 
AflIII A'CryG_T

Cuts at:   4401    5505    6482    4401 
   Size:       1104     977    4968

  Fragments arranged by size: 

                4968    1104     977
 
AgeI A'CCGG_T

Cuts at:   5437    5437 
   Size:       7049
 
AhdI GACnn_n'nnGTC

Cuts at:   3351    3351 
   Size:       7049
 
AleI CACnn'nnGTG

Cuts at:    151     151 
   Size:       7049
 
AlwNI CAG_nnn'CTG

Cuts at:   2947    3208    3973    4354    5921    2947 
   Size:        261     765     381    1567    4075

  Fragments arranged by size: 

                4075    1567     765     381     261
 
ApaI G_GGCC'C

Cuts at:    283     283 
   Size:       7049
 
ApaLI G'TGCA_C

Cuts at:   5819    5819 
   Size:       7049
 
ApoI r'AATT_y

Cuts at:    769     868    1060    1116    1230    1315    1527    3136    4053 
   Size:         99     192      56     114      85     212    1609     917

Cuts at:   4053    6402    6658     769 
   Size:       2349     256    1160

  Fragments arranged by size: 

                2349    1609    1160     917     256     212     192     114
                  99      85      56
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       7049
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7049
 
AvaI C'yCGr_G

Cuts at:   4069    4069 
   Size:       7049
 
BamHI G'GATC_C

Cuts at:   3708    4074    3708 
   Size:        366    6683
 
BanI G'GyrC_C

Cuts at:   4065    4479    4727    4762    4065 
   Size:        414     248      35    6352

  Fragments arranged by size: 

                6352     414     248      35
 
BanII G_rGCy'C

Cuts at:    283    3341    3400    4063    5093     283 
   Size:       3058      59     663    1030    2239

  Fragments arranged by size: 

                3058    2239    1030     663      59
 
BbeI G_GCGC'C

Cuts at:   4483    4731    4483 
   Size:        248    6801
 
BbsI GAAGACnn'nnnn_

Cuts at:    409     560     910    1598    2258    3227     409 
   Size:        151     350     688     660     969    4231

  Fragments arranged by size: 

                4231     969     688     660     350     151
 
BbvCI CC'TCA_GC

Cuts at:   1667    1667 
   Size:       7049
 
BciVI GTATCCnnnnn_n'

Cuts at:   1237    2154    4947    5708    7049    1237 
   Size:        917    2793     761    1341    1237

  Fragments arranged by size: 

                2793    1341    1237     917     761
 
BclI T'GATC_A

Cuts at:   1557    2322    4569    1557 
   Size:        765    2247    4037

  Fragments arranged by size: 

                4037    2247     765
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       7049
 
BglII A'GATC_T

Cuts at:    541    1209    4564    6601     541 
   Size:        668    3355    2037     989

  Fragments arranged by size: 

                3355    2037     989     668
 
BlpI GC'TnA_GC

Cuts at:   4161    4161 
   Size:       7049
 
Bme1580I G_kGCm'C

Cuts at:    148     283    4674    4767    5823     148 
   Size:        135    4391      93    1056    1374

  Fragments arranged by size: 

                4391    1374    1056     135      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   3041    3722    4261    4342    4677    6821    3041 
   Size:        681     539      81     335    2144    3269

  Fragments arranged by size: 

                3269    2144     681     539     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2297    5416    2297 
   Size:       3119    3930
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    491     564    1709    2426    3056    3334    3662     491 
   Size:         73    1145     717     630     278     328    3878

  Fragments arranged by size: 

                3878    1145     717     630     328     278      73
 
Bpu10I CC'TnA_GC

Cuts at:    491    1667    3473     491 
   Size:       1176    1806    4067

  Fragments arranged by size: 

                4067    1806    1176
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2267    3384    3703    4225    5104    5596    5894    2267 
   Size:       1117     319     522     879     492     298    3422

  Fragments arranged by size: 

                3422    1117     879     522     492     319     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   6753    6753 
   Size:       7049
 
BsaAI yAC'GTr

Cuts at:   4402    4402 
   Size:       7049
 
BsaBI GATnn'nnATC

Cuts at:      6    3707    3713    4563    6600       6 
   Size:       3701       6     850    2037     455

  Fragments arranged by size: 

                3701    2037     850     455       6
 
BsaHI Gr'CG_yC

Cuts at:   4480    4728    5430    6926    4480 
   Size:        248     702    1496    4603

  Fragments arranged by size: 

                4603    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   4238    4759    5437    5711    5858    6269    4238 
   Size:        521     678     274     147     411    5018

  Fragments arranged by size: 

                5018     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    287     317    1792    1822    3642    3660    3672    3690    3879 
   Size:         30    1475      30    1820      18      12      18     189

Cuts at:   3879    3909     287 
   Size:         30    3427

  Fragments arranged by size: 

                3427    1820    1475     189      30      30      30      18
                  18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    185     967     970    1303    1447    1450    1753    1756    1759 
   Size:        782       3     333     144       3     303       3       3

Cuts at:   1759    1762    1813    3004    3019    3046    3494    3900    3930 
   Size:          3      51    1191      15      27     448     406      30

Cuts at:   3930     185 
   Size:       3304

  Fragments arranged by size: 

                3304    1191     782     448     406     333     303     144
                  51      30      27      15       3       3       3       3
                   3
 
BseYI C'CCAG_C

Cuts at:   3331    4474    5232    5809    6305    3331 
   Size:       1143     758     577     496    4075

  Fragments arranged by size: 

                4075    1143     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    803    4059     803 
   Size:       3256    3793
 
BsiEI CG_ry'CG

Cuts at:    104    4591    4637    5845     104 
   Size:       4487      46    1208    1308

  Fragments arranged by size: 

                4487    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   2283    3341    3400    4063    4841    5031    5823    2283 
   Size:       1058      59     663     778     190     792    3509

  Fragments arranged by size: 

                3509    1058     792     778     663     190      59
 
BsmBI CGTCTCn'nnnn_

Cuts at:    405     405 
   Size:       7049
 
Bsp1286I G_dGCh'C

Cuts at:    148     283    2283    3341    3400    4063    4674    4767    4841 
   Size:        135    2000    1058      59     663     611      93      74

Cuts at:   4841    5031    5093    5823     148 
   Size:        190      62     730    1374

  Fragments arranged by size: 

                2000    1374    1058     730     663     611     190     135
                  93      74      62      59
 
BspEI T'CCGG_A

Cuts at:   6269    6269 
   Size:       7049
 
BspHI T'CATG_A

Cuts at:   6225    6225 
   Size:       7049
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1995    3367    4099    4313    4615    4996    1995 
   Size:       1372     732     214     302     381    4048

  Fragments arranged by size: 

                4048    1372     732     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    5341    5395    6879    7044      50 
   Size:       5291      54    1484     165      55

  Fragments arranged by size: 

                5291    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1327    1360    4961    1327 
   Size:         33    3601    3415

  Fragments arranged by size: 

                3601    3415      33
 
BsrFI r'CCGG_y

Cuts at:   3224    5047    5228    5437    3224 
   Size:       1823     181     209    4836

  Fragments arranged by size: 

                4836    1823     209     181
 
BsrGI T'GTAC_A

Cuts at:   1055    1146    3445    1055 
   Size:         91    2299    4659

  Fragments arranged by size: 

                4659    2299      91
 
BssSI C'ACGA_G

Cuts at:   5320    5678    5320 
   Size:        358    6691
 
BstAPI GCAn_nnn'nTGC

Cuts at:   4426    4426 
   Size:       7049
 
BstBI TT'CG_AA

Cuts at:   3282    5410    3282 
   Size:       2128    4921
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1213    1213 
   Size:       7049
 
BstYI r'GATC_y

Cuts at:      1     541    1209    2154    3708    4074    4541    4564    4899 
   Size:        540     668     945    1554     366     467      23     335

Cuts at:   4899    5145    6157    6243    6255    6601       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1554    1012     945     668     540     467     449     366
                 346     335     246      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4999    5180    5343    4999 
   Size:        181     163    6705

  Fragments arranged by size: 

                6705     181     163
 
BtsI GCAGTG_nn'

Cuts at:    815     939     999    3010    4292     815 
   Size:        124      60    2011    1282    3572

  Fragments arranged by size: 

                3572    2011    1282     124      60
 
ClaI AT'CG_AT

Cuts at:   3735    3735 
   Size:       7049
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   3299    3334    3299 
   Size:         35    7014
 
DraI TTT'AAA

Cuts at:    842    2834    3899    4048     842 
   Size:       1992    1065     149    3843

  Fragments arranged by size: 

                3843    1992    1065     149
 
DraIII CAC_nnn'GTG

Cuts at:   3049    3049 
   Size:       7049
 
DrdI GACnn_nn'nnGTC

Cuts at:   4755    5613    4755 
   Size:        858    6191
 
EaeI y'GGCC_r

Cuts at:   4634    4808    5199    5226    6571    4634 
   Size:        174     391      27    1345    5112

  Fragments arranged by size: 

                5112    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   4634    4634 
   Size:       7049
 
EarI CTCTTCn'nnn_

Cuts at:    557     944    1730    1757    1790    2594    2630    2694    3023 
   Size:        387     786      27      33     804      36      64     329

Cuts at:   3023    3269    3948    5072    5282     557 
   Size:        246     679    1124     210    2324

  Fragments arranged by size: 

                2324    1124     804     786     679     387     329     246
                 210      64      36      33      27
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   5257    5567    5713    6859    5257 
   Size:        310     146    1146    5447

  Fragments arranged by size: 

                5447    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   3339    3398    4061    3339 
   Size:         59     663    6327

  Fragments arranged by size: 

                6327     663      59
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    491     564     761    1030    1709    2270    2426    2675    3047 
   Size:         73     197     269     679     561     156     249     372

Cuts at:   3047    3056    3334    3377    3662    3884    4874    5306    6053 
   Size:          9     278      43     285     222     990     432     747

Cuts at:   6053    6321     491 
   Size:        268    1219

  Fragments arranged by size: 

                1219     990     747     679     561     432     372     285
                 278     269     268     249     222     197     156      73
                  43       9
 
EcoO109I rG'GnC_Cy

Cuts at:    114     279    1911    4188     114 
   Size:        165    1632    2277    2975

  Fragments arranged by size: 

                2975    2277    1632     165
 
EcoRI G'AATT_C

Cuts at:   4053    4053 
   Size:       7049
 
EcoRV GAT'ATC

Cuts at:   1111    1111 
   Size:       7049
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    867    2677    3841    5068     867 
   Size:       1810    1164    1227    2848

  Fragments arranged by size: 

                2848    1810    1227    1164
 
FspI TGC'GCA

Cuts at:   4830    4830 
   Size:       7049
 
HaeII r_GCGC'y

Cuts at:   2100    4483    4731    5753    6732    2100 
   Size:       2383     248    1022     979    2417

  Fragments arranged by size: 

                2417    2383    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    550     556     582     588    2001    2033    2875    2907    3337 
   Size:          6      26       6    1413      32     842      32     430

Cuts at:   3337    3369    3660    3692    4891    4921    4923    4953     550 
   Size:         32     291      32    1199      30       2      30    2646

  Fragments arranged by size: 

                2646    1413    1199     842     430     291      32      32
                  32      32      30      30      26       6       6       2
            
 
HincII GTy'rAC

Cuts at:   1347    1575    3983    4088    1347 
   Size:        228    2408     105    4308

  Fragments arranged by size: 

                4308    2408     228     105
 
HindIII A'AGCT_T

Cuts at:   2231    2231 
   Size:       7049
 
HpaI GTT'AAC

Cuts at:   1347    1575    3983    1347 
   Size:        228    2408    4413

  Fragments arranged by size: 

                4413    2408     228
 
KasI G'GCGC_C

Cuts at:   4479    4727    4479 
   Size:        248    6801
 
KpnI G_GTAC'C

Cuts at:   4069    4069 
   Size:       7049
 
MfeI C'AATT_G

Cuts at:   1051    3311    1051 
   Size:       2260    4789
 
MluI A'CGCG_T

Cuts at:   6482    6482 
   Size:       7049
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3752    5720    5904    3752 
   Size:       1968     184    4897

  Fragments arranged by size: 

                4897    1968     184
 
MscI TGG'CCA

Cuts at:   4810    4810 
   Size:       7049
 
MslI CAynn'nnrTG

Cuts at:    151    3634    5038    5165     151 
   Size:       3483    1404     127    2035

  Fragments arranged by size: 

                3483    2035    1404     127
 
MspA1I CmG'CkG

Cuts at:   1237    3586    3970    4160    4474    4834    5847    6092    1237 
   Size:       2349     384     190     314     360    1013     245    2194

  Fragments arranged by size: 

                2349    2194    1013     384     360     314     245     190
            
 
NaeI GCC'GGC

Cuts at:   3226    5230    3226 
   Size:       2004    5045
 
NarI GG'CG_CC

Cuts at:   4480    4728    4480 
   Size:        248    6801
 
NgoMIV G'CCGG_C

Cuts at:   3224    5228    3224 
   Size:       2004    5045
 
NspI r_CATG'y

Cuts at:   4102    4602    5509    4102 
   Size:        500     907    5642

  Fragments arranged by size: 

                5642     907     500
 
PciI A'CATG_T

Cuts at:   5505    5505 
   Size:       7049
 
PflMI CCAn_nnn'nTGG

Cuts at:   3148    3148 
   Size:       7049
 
PfoI T'CCnGG_A

Cuts at:   3678    3678 
   Size:       7049
 
PmeI GTTT'AAAC

Cuts at:   4048    4048 
   Size:       7049
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6188    6220    6188 
   Size:         32    7017
 
PpuMI rG'GwC_Cy

Cuts at:   1911    1911 
   Size:       7049
 
PsiI TTA'TAA

Cuts at:   1265    1265 
   Size:       7049
 
PspOMI G'GGCC_C

Cuts at:    279     279 
   Size:       7049
 
PstI C_TGCA'G

Cuts at:   1242    2964    3017    4096    4781    1242 
   Size:       1722      53    1079     685    3510

  Fragments arranged by size: 

                3510    1722    1079     685      53
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       7049
 
PvuII CAG'CTG

Cuts at:   1237    3586    3970    4474    4834    1237 
   Size:       2349     384     504     360    3452

  Fragments arranged by size: 

                3452    2349     504     384     360
 
RsrII CG'GwC_CG

Cuts at:   5244    5244 
   Size:       7049
 
SacI G_AGCT'C

Cuts at:   3341    3400    4063    3341 
   Size:         59     663    6327

  Fragments arranged by size: 

                6327     663      59
 
SalI G'TCGA_C

Cuts at:   4086    4086 
   Size:       7049
 
SapI GCTCTTCn'nnn_

Cuts at:   2694    5072    5282    2694 
   Size:       2378     210    4461

  Fragments arranged by size: 

                4461    2378     210
 
SbfI CC_TGCA'GG

Cuts at:   4096    4096 
   Size:       7049
 
ScaI AGT'ACT

Cuts at:   1294    6566    1294 
   Size:       5272    1777
 
SfcI C'TryA_G

Cuts at:     33     377    1238    1296    2960    3013    4092    4777    5770 
   Size:        344     861      58    1664      53    1079     685     993

Cuts at:   5770    5961      33 
   Size:        191    1121

  Fragments arranged by size: 

                1664    1121    1079     993     861     685     344     191
                  58      53
 
SfoI GGC'GCC

Cuts at:   4481    4729    4481 
   Size:        248    6801
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       7049
 
SmaI CCC'GGG

Cuts at:   4071    4071 
   Size:       7049
 
SmlI C'TyrA_G

Cuts at:   2282    3399    3718    4204    5119    5611    5873    2282 
   Size:       1117     319     486     915     492     262    3458

  Fragments arranged by size: 

                3458    1117     915     492     486     319     262
 
SpeI A'CTAG_T

Cuts at:     67     808      67 
   Size:        741    6308
 
SphI G_CATG'C

Cuts at:   4102    4602    4102 
   Size:        500    6549
 
SspI AAT'ATT

Cuts at:    741    1173    1500     741 
   Size:        432     327    6290

  Fragments arranged by size: 

                6290     432     327
 
StyI C'CwwG_G

Cuts at:   4183    4537    4183 
   Size:        354    6695
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   3060    4632    4765    5224    5433    3060 
   Size:       1572     133     459     209    4676

  Fragments arranged by size: 

                4676    1572     459     209     133
 
TatI w'GTAC_w

Cuts at:   1055    1101    1146    1292    3445    6564    1055 
   Size:         46      45     146    2153    3119    1540

  Fragments arranged by size: 

                3119    2153    1540     146      46      45
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    287     319    1792    1824    1928    1960    6566    6598     287 
   Size:         32    1473      32     104      32    4606      32     738

  Fragments arranged by size: 

                4606    1473     738     104      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   3672    4846    3672 
   Size:       1174    5875
 
XbaI T'CTAG_A

Cuts at:   4080    4080 
   Size:       7049
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1840    4544    1840 
   Size:       2704    4345
 
XmaI C'CCGG_G

Cuts at:   4069    4069 
   Size:       7049
 
XmnI GAAnn'nnTTC

Cuts at:   1019    2852    3278    3824    1019 
   Size:       1833     426     546    4244

  Fragments arranged by size: 

                4244    1833     546     426
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AlfI     AloI     AscI    AvrII
     BaeI     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI   BssHII
   BstEII  BstZ17I   Bsu36I     BtgI    EcoNI     FseI    FspAI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI
    PshAI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com