(Circular) (Six-base) MAPSORT of: pF1KB0053.seq Check: 1198 from: 1 to: 7049 pF1KB0053 7049 bp With 182 enzymes: SgfI * June 20, 2008 10:22 .. AccI GT'mk_AC Cuts at: 1202 4087 1202 Size: 2885 4164 Acc65I G'GTAC_C Cuts at: 4065 4065 Size: 7049 AclI AA'CG_TT Cuts at: 6889 6889 Size: 7049 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 761 1030 2270 2675 3047 3377 3884 4874 5306 Size: 269 1240 405 372 330 507 990 432 Cuts at: 5306 6053 6321 761 Size: 747 268 1489 Fragments arranged by size: 1489 1240 990 747 507 432 405 372 330 269 268 AflIII A'CryG_T Cuts at: 4401 5505 6482 4401 Size: 1104 977 4968 Fragments arranged by size: 4968 1104 977 AgeI A'CCGG_T Cuts at: 5437 5437 Size: 7049 AhdI GACnn_n'nnGTC Cuts at: 3351 3351 Size: 7049 AleI CACnn'nnGTG Cuts at: 151 151 Size: 7049 AlwNI CAG_nnn'CTG Cuts at: 2947 3208 3973 4354 5921 2947 Size: 261 765 381 1567 4075 Fragments arranged by size: 4075 1567 765 381 261 ApaI G_GGCC'C Cuts at: 283 283 Size: 7049 ApaLI G'TGCA_C Cuts at: 5819 5819 Size: 7049 ApoI r'AATT_y Cuts at: 769 868 1060 1116 1230 1315 1527 3136 4053 Size: 99 192 56 114 85 212 1609 917 Cuts at: 4053 6402 6658 769 Size: 2349 256 1160 Fragments arranged by size: 2349 1609 1160 917 256 212 192 114 99 85 56 AseI AT'TA_AT Cuts at: 20 20 Size: 7049 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7049 AvaI C'yCGr_G Cuts at: 4069 4069 Size: 7049 BamHI G'GATC_C Cuts at: 3708 4074 3708 Size: 366 6683 BanI G'GyrC_C Cuts at: 4065 4479 4727 4762 4065 Size: 414 248 35 6352 Fragments arranged by size: 6352 414 248 35 BanII G_rGCy'C Cuts at: 283 3341 3400 4063 5093 283 Size: 3058 59 663 1030 2239 Fragments arranged by size: 3058 2239 1030 663 59 BbeI G_GCGC'C Cuts at: 4483 4731 4483 Size: 248 6801 BbsI GAAGACnn'nnnn_ Cuts at: 409 560 910 1598 2258 3227 409 Size: 151 350 688 660 969 4231 Fragments arranged by size: 4231 969 688 660 350 151 BbvCI CC'TCA_GC Cuts at: 1667 1667 Size: 7049 BciVI GTATCCnnnnn_n' Cuts at: 1237 2154 4947 5708 7049 1237 Size: 917 2793 761 1341 1237 Fragments arranged by size: 2793 1341 1237 917 761 BclI T'GATC_A Cuts at: 1557 2322 4569 1557 Size: 765 2247 4037 Fragments arranged by size: 4037 2247 765 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 7049 BglII A'GATC_T Cuts at: 541 1209 4564 6601 541 Size: 668 3355 2037 989 Fragments arranged by size: 3355 2037 989 668 BlpI GC'TnA_GC Cuts at: 4161 4161 Size: 7049 Bme1580I G_kGCm'C Cuts at: 148 283 4674 4767 5823 148 Size: 135 4391 93 1056 1374 Fragments arranged by size: 4391 1374 1056 135 93 BmrI ACTGGGnnnn_n' Cuts at: 3041 3722 4261 4342 4677 6821 3041 Size: 681 539 81 335 2144 3269 Fragments arranged by size: 3269 2144 681 539 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2297 5416 2297 Size: 3119 3930 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 491 564 1709 2426 3056 3334 3662 491 Size: 73 1145 717 630 278 328 3878 Fragments arranged by size: 3878 1145 717 630 328 278 73 Bpu10I CC'TnA_GC Cuts at: 491 1667 3473 491 Size: 1176 1806 4067 Fragments arranged by size: 4067 1806 1176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2267 3384 3703 4225 5104 5596 5894 2267 Size: 1117 319 522 879 492 298 3422 Fragments arranged by size: 3422 1117 879 522 492 319 298 BsaI GGTCTCn'nnnn_ Cuts at: 6753 6753 Size: 7049 BsaAI yAC'GTr Cuts at: 4402 4402 Size: 7049 BsaBI GATnn'nnATC Cuts at: 6 3707 3713 4563 6600 6 Size: 3701 6 850 2037 455 Fragments arranged by size: 3701 2037 850 455 6 BsaHI Gr'CG_yC Cuts at: 4480 4728 5430 6926 4480 Size: 248 702 1496 4603 Fragments arranged by size: 4603 1496 702 248 BsaWI w'CCGG_w Cuts at: 4238 4759 5437 5711 5858 6269 4238 Size: 521 678 274 147 411 5018 Fragments arranged by size: 5018 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 287 317 1792 1822 3642 3660 3672 3690 3879 Size: 30 1475 30 1820 18 12 18 189 Cuts at: 3879 3909 287 Size: 30 3427 Fragments arranged by size: 3427 1820 1475 189 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 185 967 970 1303 1447 1450 1753 1756 1759 Size: 782 3 333 144 3 303 3 3 Cuts at: 1759 1762 1813 3004 3019 3046 3494 3900 3930 Size: 3 51 1191 15 27 448 406 30 Cuts at: 3930 185 Size: 3304 Fragments arranged by size: 3304 1191 782 448 406 333 303 144 51 30 27 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3331 4474 5232 5809 6305 3331 Size: 1143 758 577 496 4075 Fragments arranged by size: 4075 1143 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 803 4059 803 Size: 3256 3793 BsiEI CG_ry'CG Cuts at: 104 4591 4637 5845 104 Size: 4487 46 1208 1308 Fragments arranged by size: 4487 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 2283 3341 3400 4063 4841 5031 5823 2283 Size: 1058 59 663 778 190 792 3509 Fragments arranged by size: 3509 1058 792 778 663 190 59 BsmBI CGTCTCn'nnnn_ Cuts at: 405 405 Size: 7049 Bsp1286I G_dGCh'C Cuts at: 148 283 2283 3341 3400 4063 4674 4767 4841 Size: 135 2000 1058 59 663 611 93 74 Cuts at: 4841 5031 5093 5823 148 Size: 190 62 730 1374 Fragments arranged by size: 2000 1374 1058 730 663 611 190 135 93 74 62 59 BspEI T'CCGG_A Cuts at: 6269 6269 Size: 7049 BspHI T'CATG_A Cuts at: 6225 6225 Size: 7049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1995 3367 4099 4313 4615 4996 1995 Size: 1372 732 214 302 381 4048 Fragments arranged by size: 4048 1372 732 381 302 214 BsrBI CCG'CTC Cuts at: 50 5341 5395 6879 7044 50 Size: 5291 54 1484 165 55 Fragments arranged by size: 5291 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1327 1360 4961 1327 Size: 33 3601 3415 Fragments arranged by size: 3601 3415 33 BsrFI r'CCGG_y Cuts at: 3224 5047 5228 5437 3224 Size: 1823 181 209 4836 Fragments arranged by size: 4836 1823 209 181 BsrGI T'GTAC_A Cuts at: 1055 1146 3445 1055 Size: 91 2299 4659 Fragments arranged by size: 4659 2299 91 BssSI C'ACGA_G Cuts at: 5320 5678 5320 Size: 358 6691 BstAPI GCAn_nnn'nTGC Cuts at: 4426 4426 Size: 7049 BstBI TT'CG_AA Cuts at: 3282 5410 3282 Size: 2128 4921 BstXI CCAn_nnnn'nTGG Cuts at: 1213 1213 Size: 7049 BstYI r'GATC_y Cuts at: 1 541 1209 2154 3708 4074 4541 4564 4899 Size: 540 668 945 1554 366 467 23 335 Cuts at: 4899 5145 6157 6243 6255 6601 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1554 1012 945 668 540 467 449 366 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4999 5180 5343 4999 Size: 181 163 6705 Fragments arranged by size: 6705 181 163 BtsI GCAGTG_nn' Cuts at: 815 939 999 3010 4292 815 Size: 124 60 2011 1282 3572 Fragments arranged by size: 3572 2011 1282 124 60 ClaI AT'CG_AT Cuts at: 3735 3735 Size: 7049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 3299 3334 3299 Size: 35 7014 DraI TTT'AAA Cuts at: 842 2834 3899 4048 842 Size: 1992 1065 149 3843 Fragments arranged by size: 3843 1992 1065 149 DraIII CAC_nnn'GTG Cuts at: 3049 3049 Size: 7049 DrdI GACnn_nn'nnGTC Cuts at: 4755 5613 4755 Size: 858 6191 EaeI y'GGCC_r Cuts at: 4634 4808 5199 5226 6571 4634 Size: 174 391 27 1345 5112 Fragments arranged by size: 5112 1345 391 174 27 EagI C'GGCC_G Cuts at: 4634 4634 Size: 7049 EarI CTCTTCn'nnn_ Cuts at: 557 944 1730 1757 1790 2594 2630 2694 3023 Size: 387 786 27 33 804 36 64 329 Cuts at: 3023 3269 3948 5072 5282 557 Size: 246 679 1124 210 2324 Fragments arranged by size: 2324 1124 804 786 679 387 329 246 210 64 36 33 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 5257 5567 5713 6859 5257 Size: 310 146 1146 5447 Fragments arranged by size: 5447 1146 310 146 EcoICRI GAG'CTC Cuts at: 3339 3398 4061 3339 Size: 59 663 6327 Fragments arranged by size: 6327 663 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 491 564 761 1030 1709 2270 2426 2675 3047 Size: 73 197 269 679 561 156 249 372 Cuts at: 3047 3056 3334 3377 3662 3884 4874 5306 6053 Size: 9 278 43 285 222 990 432 747 Cuts at: 6053 6321 491 Size: 268 1219 Fragments arranged by size: 1219 990 747 679 561 432 372 285 278 269 268 249 222 197 156 73 43 9 EcoO109I rG'GnC_Cy Cuts at: 114 279 1911 4188 114 Size: 165 1632 2277 2975 Fragments arranged by size: 2975 2277 1632 165 EcoRI G'AATT_C Cuts at: 4053 4053 Size: 7049 EcoRV GAT'ATC Cuts at: 1111 1111 Size: 7049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 867 2677 3841 5068 867 Size: 1810 1164 1227 2848 Fragments arranged by size: 2848 1810 1227 1164 FspI TGC'GCA Cuts at: 4830 4830 Size: 7049 HaeII r_GCGC'y Cuts at: 2100 4483 4731 5753 6732 2100 Size: 2383 248 1022 979 2417 Fragments arranged by size: 2417 2383 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 550 556 582 588 2001 2033 2875 2907 3337 Size: 6 26 6 1413 32 842 32 430 Cuts at: 3337 3369 3660 3692 4891 4921 4923 4953 550 Size: 32 291 32 1199 30 2 30 2646 Fragments arranged by size: 2646 1413 1199 842 430 291 32 32 32 32 30 30 26 6 6 2 HincII GTy'rAC Cuts at: 1347 1575 3983 4088 1347 Size: 228 2408 105 4308 Fragments arranged by size: 4308 2408 228 105 HindIII A'AGCT_T Cuts at: 2231 2231 Size: 7049 HpaI GTT'AAC Cuts at: 1347 1575 3983 1347 Size: 228 2408 4413 Fragments arranged by size: 4413 2408 228 KasI G'GCGC_C Cuts at: 4479 4727 4479 Size: 248 6801 KpnI G_GTAC'C Cuts at: 4069 4069 Size: 7049 MfeI C'AATT_G Cuts at: 1051 3311 1051 Size: 2260 4789 MluI A'CGCG_T Cuts at: 6482 6482 Size: 7049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3752 5720 5904 3752 Size: 1968 184 4897 Fragments arranged by size: 4897 1968 184 MscI TGG'CCA Cuts at: 4810 4810 Size: 7049 MslI CAynn'nnrTG Cuts at: 151 3634 5038 5165 151 Size: 3483 1404 127 2035 Fragments arranged by size: 3483 2035 1404 127 MspA1I CmG'CkG Cuts at: 1237 3586 3970 4160 4474 4834 5847 6092 1237 Size: 2349 384 190 314 360 1013 245 2194 Fragments arranged by size: 2349 2194 1013 384 360 314 245 190 NaeI GCC'GGC Cuts at: 3226 5230 3226 Size: 2004 5045 NarI GG'CG_CC Cuts at: 4480 4728 4480 Size: 248 6801 NgoMIV G'CCGG_C Cuts at: 3224 5228 3224 Size: 2004 5045 NspI r_CATG'y Cuts at: 4102 4602 5509 4102 Size: 500 907 5642 Fragments arranged by size: 5642 907 500 PciI A'CATG_T Cuts at: 5505 5505 Size: 7049 PflMI CCAn_nnn'nTGG Cuts at: 3148 3148 Size: 7049 PfoI T'CCnGG_A Cuts at: 3678 3678 Size: 7049 PmeI GTTT'AAAC Cuts at: 4048 4048 Size: 7049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6188 6220 6188 Size: 32 7017 PpuMI rG'GwC_Cy Cuts at: 1911 1911 Size: 7049 PsiI TTA'TAA Cuts at: 1265 1265 Size: 7049 PspOMI G'GGCC_C Cuts at: 279 279 Size: 7049 PstI C_TGCA'G Cuts at: 1242 2964 3017 4096 4781 1242 Size: 1722 53 1079 685 3510 Fragments arranged by size: 3510 1722 1079 685 53 PvuI CG_AT'CG Cuts at: 104 104 Size: 7049 PvuII CAG'CTG Cuts at: 1237 3586 3970 4474 4834 1237 Size: 2349 384 504 360 3452 Fragments arranged by size: 3452 2349 504 384 360 RsrII CG'GwC_CG Cuts at: 5244 5244 Size: 7049 SacI G_AGCT'C Cuts at: 3341 3400 4063 3341 Size: 59 663 6327 Fragments arranged by size: 6327 663 59 SalI G'TCGA_C Cuts at: 4086 4086 Size: 7049 SapI GCTCTTCn'nnn_ Cuts at: 2694 5072 5282 2694 Size: 2378 210 4461 Fragments arranged by size: 4461 2378 210 SbfI CC_TGCA'GG Cuts at: 4096 4096 Size: 7049 ScaI AGT'ACT Cuts at: 1294 6566 1294 Size: 5272 1777 SfcI C'TryA_G Cuts at: 33 377 1238 1296 2960 3013 4092 4777 5770 Size: 344 861 58 1664 53 1079 685 993 Cuts at: 5770 5961 33 Size: 191 1121 Fragments arranged by size: 1664 1121 1079 993 861 685 344 191 58 53 SfoI GGC'GCC Cuts at: 4481 4729 4481 Size: 248 6801 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7049 SmaI CCC'GGG Cuts at: 4071 4071 Size: 7049 SmlI C'TyrA_G Cuts at: 2282 3399 3718 4204 5119 5611 5873 2282 Size: 1117 319 486 915 492 262 3458 Fragments arranged by size: 3458 1117 915 492 486 319 262 SpeI A'CTAG_T Cuts at: 67 808 67 Size: 741 6308 SphI G_CATG'C Cuts at: 4102 4602 4102 Size: 500 6549 SspI AAT'ATT Cuts at: 741 1173 1500 741 Size: 432 327 6290 Fragments arranged by size: 6290 432 327 StyI C'CwwG_G Cuts at: 4183 4537 4183 Size: 354 6695 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 3060 4632 4765 5224 5433 3060 Size: 1572 133 459 209 4676 Fragments arranged by size: 4676 1572 459 209 133 TatI w'GTAC_w Cuts at: 1055 1101 1146 1292 3445 6564 1055 Size: 46 45 146 2153 3119 1540 Fragments arranged by size: 3119 2153 1540 146 46 45 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 287 319 1792 1824 1928 1960 6566 6598 287 Size: 32 1473 32 104 32 4606 32 738 Fragments arranged by size: 4606 1473 738 104 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 3672 4846 3672 Size: 1174 5875 XbaI T'CTAG_A Cuts at: 4080 4080 Size: 7049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1840 4544 1840 Size: 2704 4345 XmaI C'CCGG_G Cuts at: 4069 4069 Size: 7049 XmnI GAAnn'nnTTC Cuts at: 1019 2852 3278 3824 1019 Size: 1833 426 546 4244 Fragments arranged by size: 4244 1833 546 426 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AlfI AloI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I BtgI EcoNI FseI FspAI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PshAI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI