Restriction map for
pF1KB0049
(Circular) (Six-base) MAPSORT of: pF1KB0049.seq Check: 3587 from: 1 to: 8582
pF1KB0049 8582 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2822 4279 5620 2822
Size: 1457 1341 5784
Fragments arranged by size:
5784 1457 1341
Acc65I G'GTAC_C
Cuts at: 5598 5598
Size: 8582
AclI AA'CG_TT
Cuts at: 774 2537 8422 774
Size: 1763 5885 934
Fragments arranged by size:
5885 1763 934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2883 3684 3920 4747 4874 5375 5505 6407 6839
Size: 801 236 827 127 501 130 902 432
Cuts at: 6839 7586 7854 2883
Size: 747 268 3611
Fragments arranged by size:
3611 902 827 801 747 501 432 268
236 130 127
AfeI AGC'GCT
Cuts at: 162 971 162
Size: 809 7773
AflIII A'CryG_T
Cuts at: 1700 3429 3947 4700 5934 7038 8015 1700
Size: 1729 518 753 1234 1104 977 2267
Fragments arranged by size:
2267 1729 1234 1104 977 753 518
AgeI A'CCGG_T
Cuts at: 6970 6970
Size: 8582
AleI CACnn'nnGTG
Cuts at: 2437 3202 2437
Size: 765 7817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 558 1945 2378 558
Size: 1387 433 6762
Fragments arranged by size:
6762 1387 433
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1199 1231 3989 4021 5066 5098 1199
Size: 32 2758 32 1045 32 4683
Fragments arranged by size:
4683 2758 1045 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1494 2182 2298 2493 2885 5887 7454 1494
Size: 688 116 195 392 3002 1567 2622
Fragments arranged by size:
3002 2622 1567 688 392 195 116
ApaI G_GGCC'C
Cuts at: 3669 3669
Size: 8582
ApaLI G'TGCA_C
Cuts at: 403 2644 7352 403
Size: 2241 4708 1633
Fragments arranged by size:
4708 2241 1633
ApoI r'AATT_y
Cuts at: 681 2032 2057 3001 3310 4359 5586 7935 8191
Size: 1351 25 944 309 1049 1227 2349 256
Cuts at: 8191 681
Size: 1072
Fragments arranged by size:
2349 1351 1227 1072 1049 944 309 256
25
AseI AT'TA_AT
Cuts at: 20 3500 20
Size: 3480 5102
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8582
AvaI C'yCGr_G
Cuts at: 2649 2955 3215 5602 2649
Size: 306 260 2387 5629
Fragments arranged by size:
5629 2387 306 260
BamHI G'GATC_C
Cuts at: 2558 4425 5607 2558
Size: 1867 1182 5533
Fragments arranged by size:
5533 1867 1182
BanI G'GyrC_C
Cuts at: 4576 5545 5598 6012 6260 6295 4576
Size: 969 53 414 248 35 6863
Fragments arranged by size:
6863 969 414 248 53 35
BanII G_rGCy'C
Cuts at: 121 226 1433 2211 2570 3642 3669 5596 6626
Size: 105 1207 778 359 1072 27 1927 1030
Cuts at: 6626 121
Size: 2077
Fragments arranged by size:
2077 1927 1207 1072 1030 778 359 105
27
BbeI G_GCGC'C
Cuts at: 5549 6016 6264 5549
Size: 467 248 7867
Fragments arranged by size:
7867 467 248
BbsI GAAGACnn'nnnn_
Cuts at: 1622 3800 4316 4834 1622
Size: 2178 516 518 5370
Fragments arranged by size:
5370 2178 518 516
BbvCI CC'TCA_GC
Cuts at: 1852 2486 1852
Size: 634 7948
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 571 605 2331 2365 571
Size: 34 1726 34 6788
Fragments arranged by size:
6788 1726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 388 1020 5068 6480 7241 8582 388
Size: 632 4048 1412 761 1341 388
Fragments arranged by size:
4048 1412 1341 761 632 388
BclI T'GATC_A
Cuts at: 2315 3152 5397 6102 2315
Size: 837 2245 705 4795
Fragments arranged by size:
4795 2245 837 705
BfrBI ATG'CAT
Cuts at: 1975 3710 1975
Size: 1735 6847
BglI GCCn_nnn'nGGC
Cuts at: 147 2371 147
Size: 2224 6358
BglII A'GATC_T
Cuts at: 1566 3521 6097 8134 1566
Size: 1955 2576 2037 2014
Fragments arranged by size:
2576 2037 2014 1955
BlpI GC'TnA_GC
Cuts at: 120 225 1003 5694 120
Size: 105 778 4691 3008
Fragments arranged by size:
4691 3008 778 105
Bme1580I G_kGCm'C
Cuts at: 350 407 505 2229 2648 3216 3669 6207 6300
Size: 57 98 1724 419 568 453 2538 93
Cuts at: 6300 7356 350
Size: 1056 1576
Fragments arranged by size:
2538 1724 1576 1056 568 453 419 98
93 57
BmgBI CAC'GTC
Cuts at: 2684 3948 2684
Size: 1264 7318
BmrI ACTGGGnnnn_n'
Cuts at: 5506 5794 5875 6210 8354 5506
Size: 288 81 335 2144 5734
Fragments arranged by size:
5734 2144 335 288 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6949 6949
Size: 8582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 1182 1552 1555 3629 3772 4954 177
Size: 1005 370 3 2074 143 1182 3805
Fragments arranged by size:
3805 2074 1182 1005 370 143 3
Bpu10I CC'TnA_GC
Cuts at: 390 1852 2486 390
Size: 1462 634 6486
Fragments arranged by size:
6486 1462 634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5758 6637 7129 7427 5758
Size: 879 492 298 6913
Fragments arranged by size:
6913 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1498 4825 8286 1498
Size: 3327 3461 1794
Fragments arranged by size:
3461 3327 1794
BsaAI yAC'GTr
Cuts at: 1038 5079 5935 1038
Size: 4041 856 3685
Fragments arranged by size:
4041 3685 856
BsaBI GATnn'nnATC
Cuts at: 6 1120 3526 6096 8133 6
Size: 1114 2406 2570 2037 455
Fragments arranged by size:
2570 2406 2037 1114 455
BsaHI Gr'CG_yC
Cuts at: 282 800 5546 6013 6261 6963 8459 282
Size: 518 4746 467 248 702 1496 405
Fragments arranged by size:
4746 1496 702 518 467 405 248
BsaWI w'CCGG_w
Cuts at: 757 862 3271 5152 5771 6292 6970 7244 7391
Size: 105 2409 1881 619 521 678 274 147
Cuts at: 7391 7802 757
Size: 411 1537
Fragments arranged by size:
2409 1881 1537 678 619 521 411 274
147 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 329 359 3937 3967 5063 5093 5398 5428 329
Size: 30 3578 30 1096 30 305 30 3483
Fragments arranged by size:
3578 3483 1096 305 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 1057 1456 2113 3632 3650 4330 142
Size: 915 399 657 1519 18 680 4394
Fragments arranged by size:
4394 1519 915 680 657 399 18
BseYI C'CCAG_C
Cuts at: 2288 5461 6007 6765 7342 7838 2288
Size: 3173 546 758 577 496 3032
Fragments arranged by size:
3173 3032 758 577 546 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 1480 2371 842
Size: 638 891 7053
Fragments arranged by size:
7053 891 638
BsiEI CG_ry'CG
Cuts at: 104 299 2722 6124 6170 7378 104
Size: 195 2423 3402 46 1208 1308
Fragments arranged by size:
3402 2423 1308 1208 195 46
BsiHKAI G_wGCw'C
Cuts at: 121 226 407 434 483 618 1433 1714 2570
Size: 105 181 27 49 135 815 281 856
Cuts at: 2570 2648 3642 3678 4194 4511 5596 6374 6564
Size: 78 994 36 516 317 1085 778 190
Cuts at: 6564 7356 121
Size: 792 1347
Fragments arranged by size:
1347 1085 994 856 815 792 778 516
317 281 190 181 135 105 78 49
36 27
BsiWI C'GTAC_G
Cuts at: 2506 2506
Size: 8582
BsmI GAATG_Cn'
Cuts at: 874 1782 874
Size: 908 7674
BsmBI CGTCTCn'nnnn_
Cuts at: 247 2690 247
Size: 2443 6139
Bsp1286I G_dGCh'C
Cuts at: 121 226 350 407 434 483 505 618 1433
Size: 105 124 57 27 49 22 113 815
Cuts at: 1433 1714 2211 2229 2570 2648 3216 3642 3669
Size: 281 497 18 341 78 568 426 27
Cuts at: 3669 3678 4194 4511 5596 6207 6300 6374 6564
Size: 9 516 317 1085 611 93 74 190
Cuts at: 6564 6626 7356 121
Size: 62 730 1347
Fragments arranged by size:
1347 1085 815 730 611 568 516 497
426 341 317 281 190 124 113 105
93 78 74 62 57 49 27 27
22 18 9
BspEI T'CCGG_A
Cuts at: 5152 7802 5152
Size: 2650 5932
BspHI T'CATG_A
Cuts at: 4664 7758 4664
Size: 3094 5488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 965 2535 3110 5632 5846 6148 6529 965
Size: 1570 575 2522 214 302 381 3018
Fragments arranged by size:
3018 2522 1570 575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1027 4452 6874 6928 8412 8577 50
Size: 977 3425 2422 54 1484 165 55
Fragments arranged by size:
3425 2422 1484 977 165 55 54
BsrDI GCAATG_nn'
Cuts at: 315 2570 3423 3839 4473 6494 315
Size: 2255 853 416 634 2021 2403
Fragments arranged by size:
2403 2255 2021 853 634 416
BsrFI r'CCGG_y
Cuts at: 6580 6761 6970 6580
Size: 181 209 8192
Fragments arranged by size:
8192 209 181
BsrGI T'GTAC_A
Cuts at: 2696 3161 2696
Size: 465 8117
BssSI C'ACGA_G
Cuts at: 1432 6853 7211 1432
Size: 5421 358 2803
Fragments arranged by size:
5421 2803 358
BstAPI GCAn_nnn'nTGC
Cuts at: 908 1494 2035 5959 908
Size: 586 541 3924 3531
Fragments arranged by size:
3924 3531 586 541
BstBI TT'CG_AA
Cuts at: 3345 6943 3345
Size: 3598 4984
BstEII G'GTnAC_C
Cuts at: 3421 3421
Size: 8582
BstXI CCAn_nnnn'nTGG
Cuts at: 3963 4887 4978 5371 3963
Size: 924 91 393 7174
Fragments arranged by size:
7174 924 393 91
BstYI r'GATC_y
Cuts at: 1 687 1566 2558 2600 3521 4425 5607 6074
Size: 686 879 992 42 921 904 1182 467
Cuts at: 6074 6097 6432 6678 7690 7776 7788 8134 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1182 1012 992 921 904 879 686 467
449 346 335 246 86 42 23 12
BstZ17I GTA'TAC
Cuts at: 2823 4280 2823
Size: 1457 7125
Bsu36I CC'TnA_GG
Cuts at: 3038 3038
Size: 8582
BtgI C'CryG_G
Cuts at: 107 2369 3281 4620 4683 5039 5166 107
Size: 2262 912 1339 63 356 127 3523
Fragments arranged by size:
3523 2262 1339 912 356 127 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6532 6713 6876 6532
Size: 181 163 8238
Fragments arranged by size:
8238 181 163
BtsI GCAGTG_nn'
Cuts at: 405 681 830 2172 2183 2360 2432 2444 4803
Size: 276 149 1342 11 177 72 12 2359
Cuts at: 4803 5825 405
Size: 1022 3162
Fragments arranged by size:
3162 2359 1342 1022 276 177 149 72
12 11
DraI TTT'AAA
Cuts at: 5581 5581
Size: 8582
DraIII CAC_nnn'GTG
Cuts at: 5496 5514 5496
Size: 18 8564
DrdI GACnn_nn'nnGTC
Cuts at: 6288 7146 6288
Size: 858 7724
EaeI y'GGCC_r
Cuts at: 296 524 726 2372 2625 2719 3131 4686 4800
Size: 228 202 1646 253 94 412 1555 114
Cuts at: 4800 5169 6167 6341 6732 6759 8104 296
Size: 369 998 174 391 27 1345 774
Fragments arranged by size:
1646 1555 1345 998 774 412 391 369
253 228 202 174 114 94 27
EagI C'GGCC_G
Cuts at: 296 2719 6167 296
Size: 2423 3448 2711
Fragments arranged by size:
3448 2711 2423
EarI CTCTTCn'nnn_
Cuts at: 1322 1385 2424 4646 6605 6815 1322
Size: 63 1039 2222 1959 210 3089
Fragments arranged by size:
3089 2222 1959 1039 210 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6790 7100 7246 8392 6790
Size: 310 146 1146 6980
Fragments arranged by size:
6980 1146 310 146
EcoICRI GAG'CTC
Cuts at: 119 224 1431 2568 3640 5594 119
Size: 105 1207 1137 1072 1954 3107
Fragments arranged by size:
3107 1954 1207 1137 1072 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 1182 1552 1555 2883 3629 3684 3772 3920
Size: 1005 370 3 1328 746 55 88 148
Cuts at: 3920 4747 4874 4954 5375 5505 6407 6839 7586
Size: 827 127 80 421 130 902 432 747
Cuts at: 7586 7854 177
Size: 268 905
Fragments arranged by size:
1328 1005 905 902 827 747 746 432
421 370 268 148 130 127 88 80
55 3
EcoNI CCTnn'n_nnAGG
Cuts at: 546 4124 546
Size: 3578 5004
EcoO109I rG'GnC_Cy
Cuts at: 942 1590 1893 3665 3666 4876 4982 5035 5721
Size: 648 303 1772 1 1210 106 53 686
Cuts at: 5721 942
Size: 3803
Fragments arranged by size:
3803 1772 1210 686 648 303 106 53
1
EcoRI G'AATT_C
Cuts at: 5586 5586
Size: 8582
EcoRV GAT'ATC
Cuts at: 3693 3693
Size: 8582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1228 3246 3756 3817 4185 4858 6601 1228
Size: 2018 510 61 368 673 1743 3209
Fragments arranged by size:
3209 2018 1743 673 510 368 61
FspI TGC'GCA
Cuts at: 3415 6363 3415
Size: 2948 5634
HaeII r_GCGC'y
Cuts at: 132 164 973 2298 5549 6016 6264 7286 8265
Size: 32 809 1325 3251 467 248 1022 979
Cuts at: 8265 132
Size: 449
Fragments arranged by size:
3251 1325 1022 979 809 467 449 248
32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 455 487 1497 1529 1722 1754 2070 2102 2614
Size: 32 1010 32 193 32 316 32 512
Cuts at: 2614 2646 2727 2759 3937 3969 4024 4056 4101
Size: 32 81 32 1178 32 55 32 45
Cuts at: 4101 4133 5335 5367 6424 6454 6456 6486 455
Size: 32 1202 32 1057 30 2 30 2551
Fragments arranged by size:
2551 1202 1178 1057 1010 512 316 193
81 55 45 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1408 3567 5621 1408
Size: 2159 2054 4369
Fragments arranged by size:
4369 2159 2054
KasI G'GCGC_C
Cuts at: 5545 6012 6260 5545
Size: 467 248 7867
Fragments arranged by size:
7867 467 248
KpnI G_GTAC'C
Cuts at: 5602 5602
Size: 8582
MfeI C'AATT_G
Cuts at: 5393 5393
Size: 8582
MluI A'CGCG_T
Cuts at: 8015 8015
Size: 8582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 3098 4296 4511 5321 5352 5400 7253 7437
Size: 2703 1198 215 810 31 48 1853 184
Cuts at: 7437 395
Size: 1540
Fragments arranged by size:
2703 1853 1540 1198 810 215 184 48
31
MscI TGG'CCA
Cuts at: 526 728 2374 2627 3133 6343 526
Size: 202 1646 253 506 3210 2765
Fragments arranged by size:
3210 2765 1646 506 253 202
MslI CAynn'nnrTG
Cuts at: 1040 2105 2437 3202 4419 6571 6698 1040
Size: 1065 332 765 1217 2152 127 2924
Fragments arranged by size:
2924 2152 1217 1065 765 332 127
MspA1I CmG'CkG
Cuts at: 229 414 1257 1491 1539 1647 2635 3355 3455
Size: 185 843 234 48 108 988 720 100
Cuts at: 3455 5543 5693 6007 6367 7380 7625 229
Size: 2088 150 314 360 1013 245 1186
Fragments arranged by size:
2088 1186 1013 988 843 720 360 314
245 234 185 150 108 100 48
NaeI GCC'GGC
Cuts at: 6763 6763
Size: 8582
NarI GG'CG_CC
Cuts at: 5546 6013 6261 5546
Size: 467 248 7867
Fragments arranged by size:
7867 467 248
NcoI C'CATG_G
Cuts at: 107 2369 4620 4683 5039 107
Size: 2262 2251 63 356 3650
Fragments arranged by size:
3650 2262 2251 356 63
NgoMIV G'CCGG_C
Cuts at: 6761 6761
Size: 8582
NsiI A_TGCA'T
Cuts at: 1977 3712 1977
Size: 1735 6847
NspI r_CATG'y
Cuts at: 1704 3433 3580 4704 5635 6135 7042 1704
Size: 1729 147 1124 931 500 907 3244
Fragments arranged by size:
3244 1729 1124 931 907 500 147
PasI CC'CwG_GG
Cuts at: 3768 3768
Size: 8582
PciI A'CATG_T
Cuts at: 1700 3429 4700 7038 1700
Size: 1729 1271 2338 3244
Fragments arranged by size:
3244 2338 1729 1271
PfoI T'CCnGG_A
Cuts at: 1529 1571 2492 4902 1529
Size: 42 921 2410 5209
Fragments arranged by size:
5209 2410 921 42
PmeI GTTT'AAAC
Cuts at: 5581 5581
Size: 8582
PmlI CAC'GTG
Cuts at: 1038 1038
Size: 8582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 3000 4596 4628 7721 7753 2968
Size: 32 1596 32 3093 32 3797
Fragments arranged by size:
3797 3093 1596 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 942 1893 4876 942
Size: 951 2983 4648
Fragments arranged by size:
4648 2983 951
PspOMI G'GGCC_C
Cuts at: 3665 3665
Size: 8582
PstI C_TGCA'G
Cuts at: 676 2439 2742 5629 6314 676
Size: 1763 303 2887 685 2944
Fragments arranged by size:
2944 2887 1763 685 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8582
PvuII CAG'CTG
Cuts at: 1257 1539 2635 3355 6007 6367 1257
Size: 282 1096 720 2652 360 3472
Fragments arranged by size:
3472 2652 1096 720 360 282
RsrII CG'GwC_CG
Cuts at: 6777 6777
Size: 8582
SacI G_AGCT'C
Cuts at: 121 226 1433 2570 3642 5596 121
Size: 105 1207 1137 1072 1954 3107
Fragments arranged by size:
3107 1954 1207 1137 1072 105
SalI G'TCGA_C
Cuts at: 5619 5619
Size: 8582
SanDI GG'GwC_CC
Cuts at: 4876 4876
Size: 8582
SapI GCTCTTCn'nnn_
Cuts at: 1322 6605 6815 1322
Size: 5283 210 3089
Fragments arranged by size:
5283 3089 210
SbfI CC_TGCA'GG
Cuts at: 5629 5629
Size: 8582
ScaI AGT'ACT
Cuts at: 8099 8099
Size: 8582
SfcI C'TryA_G
Cuts at: 33 546 672 896 2435 2738 4941 5625 6310
Size: 513 126 224 1539 303 2203 684 685
Cuts at: 6310 7303 7494 33
Size: 993 191 1121
Fragments arranged by size:
2203 1539 1121 993 685 684 513 303
224 191 126
SfoI GGC'GCC
Cuts at: 5547 6014 6262 5547
Size: 467 248 7867
Fragments arranged by size:
7867 467 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8582
SmaI CCC'GGG
Cuts at: 5604 5604
Size: 8582
SmlI C'TyrA_G
Cuts at: 2649 5737 6652 7144 7406 2649
Size: 3088 915 492 262 3825
Fragments arranged by size:
3825 3088 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8582
SphI G_CATG'C
Cuts at: 5635 6135 5635
Size: 500 8082
SspI AAT'ATT
Cuts at: 1995 5103 1995
Size: 3108 5474
StuI AGG'CCT
Cuts at: 2677 2677
Size: 8582
StyI C'CwwG_G
Cuts at: 107 1346 2369 4620 4683 5039 5716 6070 107
Size: 1239 1023 2251 63 356 677 354 2619
Fragments arranged by size:
2619 2251 1239 1023 677 356 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1435 2069 2191 2488 2746 4083 5286 5525 6165
Size: 634 122 297 258 1337 1203 239 640
Cuts at: 6165 6298 6757 6966 1435
Size: 133 459 209 3051
Fragments arranged by size:
3051 1337 1203 640 634 459 297 258
239 209 133 122
TatI w'GTAC_w
Cuts at: 2696 3161 4025 8097 2696
Size: 465 864 4072 3181
Fragments arranged by size:
4072 3181 864 465
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 329 361 386 418 1503 1535 4898 4930 5063
Size: 32 25 32 1085 32 3363 32 133
Cuts at: 5063 5095 5498 5530 8099 8131 329
Size: 32 403 32 2569 32 780
Fragments arranged by size:
3363 2569 1085 780 403 133 32 32
32 32 32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 6379 6379
Size: 8582
XbaI T'CTAG_A
Cuts at: 2308 5085 5613 2308
Size: 2777 528 5277
Fragments arranged by size:
5277 2777 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 378 2367 5179 6077 378
Size: 1989 2812 898 2883
Fragments arranged by size:
2883 2812 1989 898
XhoI C'TCGA_G
Cuts at: 2649 2649
Size: 8582
XmaI C'CCGG_G
Cuts at: 5602 5602
Size: 8582
XmnI GAAnn'nnTTC
Cuts at: 3718 3739 4611 4660 4841 5148 3718
Size: 21 872 49 181 307 7152
Fragments arranged by size:
7152 872 307 181 49 21
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI AvrII BaeI BmtI
BssHII ClaI CspCI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PflMI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
ZraI