Restriction map for
pF1KB0031
(Circular) (Six-base) MAPSORT of: pF1KB0031.seq Check: 8026 from: 1 to: 5858
pF1KB0031 5858 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 2896 2896
Size: 5858
Acc65I G'GTAC_C
Cuts at: 2874 2874
Size: 5858
AclI AA'CG_TT
Cuts at: 2689 5698 2689
Size: 3009 2849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1033 1537 1819 2227 3683 4115 4468 5130 1033
Size: 504 282 408 1456 432 353 662 1761
Fragments arranged by size:
1761 1456 662 504 432 408 353 282
AflII C'TTAA_G
Cuts at: 1072 1072
Size: 5858
AflIII A'CryG_T
Cuts at: 2014 3210 5010 5291 2014
Size: 1196 1800 281 2581
Fragments arranged by size:
2581 1800 1196 281
AhdI GACnn_n'nnGTC
Cuts at: 1587 1587
Size: 5858
AleI CACnn'nnGTG
Cuts at: 667 667
Size: 5858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2707 2707
Size: 5858
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 419 451 419
Size: 32 5826
AlwNI CAG_nnn'CTG
Cuts at: 1202 3163 4601 1202
Size: 1961 1438 2459
Fragments arranged by size:
2459 1961 1438
ApaLI G'TGCA_C
Cuts at: 4696 4696
Size: 5858
ApoI r'AATT_y
Cuts at: 173 496 605 618 1077 1760 1986 1999 2698
Size: 323 109 13 459 683 226 13 699
Cuts at: 2698 2862 5211 5467 173
Size: 164 2349 256 564
Fragments arranged by size:
2349 699 683 564 459 323 256 226
164 109 13 13
AseI AT'TA_AT
Cuts at: 20 1178 20
Size: 1158 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5858
AvaI C'yCGr_G
Cuts at: 2615 2878 2615
Size: 263 5595
BamHI G'GATC_C
Cuts at: 2883 2883
Size: 5858
BanI G'GyrC_C
Cuts at: 2874 3288 3536 3571 2874
Size: 414 248 35 5161
Fragments arranged by size:
5161 414 248 35
BanII G_rGCy'C
Cuts at: 121 2872 3902 121
Size: 2751 1030 2077
Fragments arranged by size:
2751 2077 1030
BbeI G_GCGC'C
Cuts at: 3292 3540 3292
Size: 248 5610
BbsI GAAGACnn'nnnn_
Cuts at: 642 680 2176 2245 2531 642
Size: 38 1496 69 286 3969
Fragments arranged by size:
3969 1496 286 69 38
BbvCI CC'TCA_GC
Cuts at: 384 384
Size: 5858
BciVI GTATCCnnnnn_n'
Cuts at: 3756 4812 5858 3756
Size: 1056 1046 3756
Fragments arranged by size:
3756 1056 1046
BclI T'GATC_A
Cuts at: 3378 3378
Size: 5858
BglII A'GATC_T
Cuts at: 1512 3373 5410 1512
Size: 1861 2037 1960
Fragments arranged by size:
2037 1960 1861
BlpI GC'TnA_GC
Cuts at: 1601 2970 1601
Size: 1369 4489
Bme1580I G_kGCm'C
Cuts at: 3483 3576 4700 3483
Size: 93 1124 4641
Fragments arranged by size:
4641 1124 93
BmgBI CAC'GTC
Cuts at: 2017 2017
Size: 5858
BmrI ACTGGGnnnn_n'
Cuts at: 1278 3070 3151 3486 5630 1278
Size: 1792 81 335 2144 1506
Fragments arranged by size:
2144 1792 1506 335 81
BmtI G_CTAG'C
Cuts at: 1223 2366 1223
Size: 1143 4715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4225 4225
Size: 5858
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1613 1643 292
Size: 1321 30 4507
Fragments arranged by size:
4507 1321 30
Bpu10I CC'TnA_GC
Cuts at: 384 2803 384
Size: 2419 3439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 458 1366 2785 3034 3913 4627 4925 153
Size: 305 908 1419 249 879 714 298 1086
Fragments arranged by size:
1419 1086 908 879 714 305 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 5562 5562
Size: 5858
BsaAI yAC'GTr
Cuts at: 2023 3211 2023
Size: 1188 4670
BsaBI GATnn'nnATC
Cuts at: 6 1067 2388 3372 5409 6
Size: 1061 1321 984 2037 455
Fragments arranged by size:
2037 1321 1061 984 455
BsaHI Gr'CG_yC
Cuts at: 3289 3537 4239 5735 3289
Size: 248 702 1496 3412
Fragments arranged by size:
3412 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3047 3568 4246 4657 4804 5078 3047
Size: 521 678 411 147 274 3827
Fragments arranged by size:
3827 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 338 368 1988 2018 130
Size: 30 178 30 1620 30 3970
Fragments arranged by size:
3970 1620 178 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 111 2782 111
Size: 2671 3187
BseYI C'CCAG_C
Cuts at: 3283 4041 4706 5114 3283
Size: 758 665 408 4027
Fragments arranged by size:
4027 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 2426 890
Size: 1536 4322
BsiEI CG_ry'CG
Cuts at: 104 3400 3446 4676 104
Size: 3296 46 1230 1286
Fragments arranged by size:
3296 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 121 2872 3650 3840 4700 121
Size: 2751 778 190 860 1279
Fragments arranged by size:
2751 1279 860 778 190
BsmI GAATG_Cn'
Cuts at: 1434 2739 1434
Size: 1305 4553
Bsp1286I G_dGCh'C
Cuts at: 121 2872 3483 3576 3650 3840 3902 4700 121
Size: 2751 611 93 74 190 62 798 1279
Fragments arranged by size:
2751 1279 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 597 1065 4290 597
Size: 468 3225 2165
Fragments arranged by size:
3225 2165 468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2908 3122 3424 3805 2908
Size: 214 302 381 4961
Fragments arranged by size:
4961 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4150 4204 5688 5853 50
Size: 4100 54 1484 165 55
Fragments arranged by size:
4100 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3770 3770
Size: 5858
BsrFI r'CCGG_y
Cuts at: 2283 3856 4037 2283
Size: 1573 181 4104
Fragments arranged by size:
4104 1573 181
BssSI C'ACGA_G
Cuts at: 4129 4837 4129
Size: 708 5150
BstAPI GCAn_nnn'nTGC
Cuts at: 3235 3235
Size: 5858
BstBI TT'CG_AA
Cuts at: 1764 4219 1764
Size: 2455 3403
BstEII G'GTnAC_C
Cuts at: 2286 2286
Size: 5858
BstYI r'GATC_y
Cuts at: 1 1141 1512 2383 2595 2883 3350 3373 3708
Size: 1140 371 871 212 288 467 23 335
Cuts at: 3708 3954 4260 4272 4358 5410 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1140 1052 871 467 449 371 335 306
288 246 212 86 23 12
BtgI C'CryG_G
Cuts at: 1320 1320
Size: 5858
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3808 3989 4152 3808
Size: 181 163 5514
Fragments arranged by size:
5514 181 163
BtsI GCAGTG_nn'
Cuts at: 1320 3101 1320
Size: 1781 4077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1196 1231 1196
Size: 35 5823
DraI TTT'AAA
Cuts at: 2857 2857
Size: 5858
DraIII CAC_nnn'GTG
Cuts at: 243 243
Size: 5858
DrdI GACnn_nn'nnGTC
Cuts at: 3564 4908 3564
Size: 1344 4514
EaeI y'GGCC_r
Cuts at: 3443 3617 4008 4035 5380 3443
Size: 174 391 27 1345 3921
Fragments arranged by size:
3921 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3443 3443
Size: 5858
EarI CTCTTCn'nnn_
Cuts at: 109 344 746 815 857 1541 1640 2058 2069
Size: 235 402 69 42 684 99 418 11
Cuts at: 2069 2222 2246 3881 4091 109
Size: 153 24 1635 210 1876
Fragments arranged by size:
1876 1635 684 418 402 235 210 153
99 69 42 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 223 4066 4808 4954 5668 223
Size: 3843 742 146 714 413
Fragments arranged by size:
3843 742 714 413 146
EcoICRI GAG'CTC
Cuts at: 119 2870 119
Size: 2751 3107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1033 1537 1613 1643 1819 2227 3683 4115
Size: 741 504 76 30 176 408 1456 432
Cuts at: 4115 4468 5130 292
Size: 353 662 1020
Fragments arranged by size:
1456 1020 741 662 504 432 408 353
176 76 30
EcoO109I rG'GnC_Cy
Cuts at: 2997 2997
Size: 5858
EcoRI G'AATT_C
Cuts at: 173 1760 2862 173
Size: 1587 1102 3169
Fragments arranged by size:
3169 1587 1102
EcoRV GAT'ATC
Cuts at: 2196 2196
Size: 5858
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2257 2818 3877 2152
Size: 105 561 1059 4133
Fragments arranged by size:
4133 1059 561 105
FspI TGC'GCA
Cuts at: 3639 3639
Size: 5858
HaeII r_GCGC'y
Cuts at: 3292 3540 4770 5541 3292
Size: 248 1230 771 3609
Fragments arranged by size:
3609 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 367 399 751 783 1369 1401 1573 1605 1720
Size: 32 352 32 586 32 172 32 115
Cuts at: 1720 1752 2199 2231 2351 2383 3700 3730 3732
Size: 32 447 32 120 32 1317 30 2
Cuts at: 3732 3762 367
Size: 30 2463
Fragments arranged by size:
2463 1317 586 447 352 172 120 115
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2272 2897 2272
Size: 625 5233
HindIII A'AGCT_T
Cuts at: 2043 2234 2800 2043
Size: 191 566 5101
Fragments arranged by size:
5101 566 191
KasI G'GCGC_C
Cuts at: 3288 3536 3288
Size: 248 5610
KpnI G_GTAC'C
Cuts at: 2878 2878
Size: 5858
MluI A'CGCG_T
Cuts at: 5291 5291
Size: 5858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1706 4617 4801 1706
Size: 2911 184 2763
Fragments arranged by size:
2911 2763 184
MscI TGG'CCA
Cuts at: 3619 3619
Size: 5858
MslI CAynn'nnrTG
Cuts at: 667 1130 2069 3847 3974 667
Size: 463 939 1778 127 2551
Fragments arranged by size:
2551 1778 939 463 127
MspA1I CmG'CkG
Cuts at: 344 1024 1605 1821 2969 3283 3643 4427 4672
Size: 680 581 216 1148 314 360 784 245
Cuts at: 4672 344
Size: 1530
Fragments arranged by size:
1530 1148 784 680 581 360 314 245
216
NaeI GCC'GGC
Cuts at: 4039 4039
Size: 5858
NarI GG'CG_CC
Cuts at: 3289 3537 3289
Size: 248 5610
NgoMIV G'CCGG_C
Cuts at: 4037 4037
Size: 5858
NheI G'CTAG_C
Cuts at: 1219 2362 1219
Size: 1143 4715
NspI r_CATG'y
Cuts at: 891 2911 3411 5014 891
Size: 2020 500 1603 1735
Fragments arranged by size:
2020 1735 1603 500
PciI A'CATG_T
Cuts at: 5010 5010
Size: 5858
PflMI CCAn_nnn'nTGG
Cuts at: 243 1618 243
Size: 1375 4483
PfoI T'CCnGG_A
Cuts at: 926 926
Size: 5858
PmeI GTTT'AAAC
Cuts at: 2857 2857
Size: 5858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 4304
Size: 32 5826
PsiI TTA'TAA
Cuts at: 482 2824 482
Size: 2342 3516
PstI C_TGCA'G
Cuts at: 194 2526 2905 3590 194
Size: 2332 379 685 2462
Fragments arranged by size:
2462 2332 685 379
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5858
PvuII CAG'CTG
Cuts at: 1024 1605 1821 3283 3643 1024
Size: 581 216 1462 360 3239
Fragments arranged by size:
3239 1462 581 360 216
RsrII CG'GwC_CG
Cuts at: 4053 4053
Size: 5858
SacI G_AGCT'C
Cuts at: 121 2872 121
Size: 2751 3107
SalI G'TCGA_C
Cuts at: 2895 2895
Size: 5858
SapI GCTCTTCn'nnn_
Cuts at: 109 1541 2058 3881 4091 109
Size: 1432 517 1823 210 1876
Fragments arranged by size:
1876 1823 1432 517 210
SbfI CC_TGCA'GG
Cuts at: 2905 2905
Size: 5858
ScaI AGT'ACT
Cuts at: 5375 5375
Size: 5858
SfcI C'TryA_G
Cuts at: 33 190 992 1289 1896 2522 2901 3586 4554
Size: 157 802 297 607 626 379 685 968
Cuts at: 4554 4745 33
Size: 191 1146
Fragments arranged by size:
1146 968 802 685 626 607 379 297
191 157
SfoI GGC'GCC
Cuts at: 3290 3538 3290
Size: 248 5610
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5858
SmaI CCC'GGG
Cuts at: 2880 2880
Size: 5858
SmlI C'TyrA_G
Cuts at: 132 473 1072 1345 2615 2764 3013 3928 4642
Size: 341 599 273 1270 149 249 915 714
Cuts at: 4642 4904 132
Size: 262 1086
Fragments arranged by size:
1270 1086 915 714 599 341 273 262
249 149
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5858
SphI G_CATG'C
Cuts at: 2911 3411 2911
Size: 500 5358
SspI AAT'ATT
Cuts at: 167 1360 167
Size: 1193 4665
StyI C'CwwG_G
Cuts at: 1640 2093 2291 2379 2992 3346 1640
Size: 453 198 88 613 354 4152
Fragments arranged by size:
4152 613 453 354 198 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 973 3441 3574 4033 4242 973
Size: 2468 133 459 209 2589
Fragments arranged by size:
2589 2468 459 209 133
TatI w'GTAC_w
Cuts at: 2526 2771 2780 5373 2526
Size: 245 9 2593 3011
Fragments arranged by size:
3011 2593 245 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 338 370 1474 1506 2270 2302 5375 5407 338
Size: 32 1104 32 764 32 3073 32 789
Fragments arranged by size:
3073 1104 789 764 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3655 3655
Size: 5858
XbaI T'CTAG_A
Cuts at: 2058 2889 2058
Size: 831 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2217 3353 2217
Size: 1136 4722
XhoI C'TCGA_G
Cuts at: 2615 2615
Size: 5858
XmaI C'CCGG_G
Cuts at: 2878 2878
Size: 5858
XmnI GAAnn'nnTTC
Cuts at: 854 1121 2144 854
Size: 267 1023 4568
Fragments arranged by size:
4568 1023 267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BsiWI BsmBI BspEI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI EcoNI FseI FspAI HpaI
MfeI NcoI NdeI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI ZraI