Restriction map for
pF1KB0023
(Circular) (Six-base) MAPSORT of: pF1KB0023.seq Check: 9566 from: 1 to: 9458
pF1KB0023 9458 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2875 4746 2875
Size: 1871 7587
AatII G_ACGT'C
Cuts at: 5495 5495
Size: 9458
AccI GT'mk_AC
Cuts at: 159 6496 159
Size: 6337 3121
Acc65I G'GTAC_C
Cuts at: 6474 6474
Size: 9458
AclI AA'CG_TT
Cuts at: 9298 9298
Size: 9458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 623 784 1015 1033 1240 1699 1807 1963
Size: 357 161 231 18 207 459 108 156
Cuts at: 1963 2344 2474 3397 3743 4013 4447 4843 5078
Size: 381 130 923 346 270 434 396 235
Cuts at: 5078 5119 5929 6240 7283 7715 8068 8730 266
Size: 41 810 311 1043 432 353 662 994
Fragments arranged by size:
1043 994 923 810 662 459 434 432
396 381 357 353 346 311 270 235
231 207 161 156 130 108 41 18
AfeI AGC'GCT
Cuts at: 3005 3005
Size: 9458
AflII C'TTAA_G
Cuts at: 1893 1893
Size: 9458
AflIII A'CryG_T
Cuts at: 162 6810 8610 8891 162
Size: 6648 1800 281 729
Fragments arranged by size:
6648 1800 729 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 1865 4250 325
Size: 1540 2385 5533
Fragments arranged by size:
5533 2385 1540
AlwNI CAG_nnn'CTG
Cuts at: 452 1011 1497 1860 2652 3831 3900 4050 4173
Size: 559 486 363 792 1179 69 150 123
Cuts at: 4173 4215 4383 4500 4821 5394 6763 8201 452
Size: 42 168 117 321 573 1369 1438 1709
Fragments arranged by size:
1709 1438 1369 1179 792 573 559 486
363 321 168 150 123 117 69 42
ApaI G_GGCC'C
Cuts at: 1590 2793 4314 5793 6100 1590
Size: 1203 1521 1479 307 4948
Fragments arranged by size:
4948 1521 1479 1203 307
ApaLI G'TGCA_C
Cuts at: 8296 8296
Size: 9458
ApoI r'AATT_y
Cuts at: 473 502 5203 6462 8811 9067 473
Size: 29 4701 1259 2349 256 864
Fragments arranged by size:
4701 2349 1259 864 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 9458
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9458
AvaI C'yCGr_G
Cuts at: 125 1997 2909 2953 3556 3575 3749 4150 4523
Size: 1872 912 44 603 19 174 401 373
Cuts at: 4523 4613 5505 6155 6428 6478 125
Size: 90 892 650 273 50 3105
Fragments arranged by size:
3105 1872 912 892 650 603 401 373
273 174 90 50 44 19
BamHI G'GATC_C
Cuts at: 6483 6483
Size: 9458
BanI G'GyrC_C
Cuts at: 2590 4648 5350 5953 6239 6418 6474 6888 7136
Size: 2058 702 603 286 179 56 414 248
Cuts at: 7136 7171 2590
Size: 35 4877
Fragments arranged by size:
4877 2058 702 603 414 286 248 179
56 35
BanII G_rGCy'C
Cuts at: 436 611 932 1205 1528 1590 1628 2702 2738
Size: 175 321 273 323 62 38 1074 36
Cuts at: 2738 2793 3429 4314 5243 5793 6089 6100 6472
Size: 55 636 885 929 550 296 11 372
Cuts at: 6472 7502 436
Size: 1030 2392
Fragments arranged by size:
2392 1074 1030 929 885 636 550 372
323 321 296 273 175 62 55 38
36 11
BbeI G_GCGC'C
Cuts at: 2594 6243 6892 7140 2594
Size: 3649 649 248 4912
Fragments arranged by size:
4912 3649 649 248
BbsI GAAGACnn'nnnn_
Cuts at: 1021 5107 5487 5917 1021
Size: 4086 380 430 4562
Fragments arranged by size:
4562 4086 430 380
BbvCI CC'TCA_GC
Cuts at: 1540 4583 1540
Size: 3043 6415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 335 832 866 5869 5903 301
Size: 34 497 34 5003 34 3856
Fragments arranged by size:
5003 3856 497 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7356 8412 9458 7356
Size: 1056 1046 7356
Fragments arranged by size:
7356 1056 1046
BclI T'GATC_A
Cuts at: 1508 6978 1508
Size: 5470 3988
BglI GCCn_nnn'nGGC
Cuts at: 1576 1606 2443 2749 2958 3577 3745 4228 1576
Size: 30 837 306 209 619 168 483 6806
Fragments arranged by size:
6806 837 619 483 306 209 168 30
BglII A'GATC_T
Cuts at: 6973 9010 6973
Size: 2037 7421
BlpI GC'TnA_GC
Cuts at: 1204 1463 1774 2050 2732 3563 3581 4064 4196
Size: 259 311 276 682 831 18 483 132
Cuts at: 4196 4376 4417 4928 5249 5719 6570 1204
Size: 180 41 511 321 470 851 4092
Fragments arranged by size:
4092 851 831 682 511 483 470 321
311 276 259 180 132 41 18
Bme1580I G_kGCm'C
Cuts at: 1590 2010 2793 3156 3647 4314 5793 5824 5899
Size: 420 783 363 491 667 1479 31 75
Cuts at: 5899 5956 6043 6100 7083 7176 8300 1590
Size: 57 87 57 983 93 1124 2748
Fragments arranged by size:
2748 1479 1124 983 783 667 491 420
363 93 87 75 57 57 31
BmrI ACTGGGnnnn_n'
Cuts at: 462 4896 6670 6751 7086 9230 462
Size: 4434 1774 81 335 2144 690
Fragments arranged by size:
4434 2144 1774 690 335 81
BmtI G_CTAG'C
Cuts at: 820 1714 5648 820
Size: 894 3934 4630
Fragments arranged by size:
4630 3934 894
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1587 3588 4071 7825 1587
Size: 2001 483 3754 3220
Fragments arranged by size:
3754 3220 2001 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 692 826 832 946 1213 1444 1582 1945
Size: 511 134 6 114 267 231 138 363
Cuts at: 1945 1954 1978 2056 2191 2203 2428 2686 2746
Size: 9 24 78 135 12 225 258 60
Cuts at: 2746 3193 3343 3367 3493 3619 3853 3922 4282
Size: 447 150 24 126 126 234 69 360
Cuts at: 4282 4642 4687 4858 5041 5176 5384 5437 5485
Size: 360 45 171 183 135 208 53 48
Cuts at: 5485 5780 5947 6002 6197 181
Size: 295 167 55 195 3442
Fragments arranged by size:
3442 511 447 363 360 360 295 267
258 234 231 225 208 195 183 171
167 150 138 135 135 134 126 126
114 78 69 60 55 53 48 45
24 24 12 9 6
Bpu10I CC'TnA_GC
Cuts at: 1540 3420 3660 4583 4838 5840 5944 1540
Size: 1880 240 923 255 1002 104 5054
Fragments arranged by size:
5054 1880 1002 923 255 240 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1900 3217 5286 6634 7513 8227 8525 961
Size: 939 1317 2069 1348 879 714 298 1894
Fragments arranged by size:
2069 1894 1348 1317 939 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1064 2326 2860 4564 5186 5978 5989 6081 6152
Size: 1262 534 1704 622 792 11 92 71
Cuts at: 6152 9162 1064
Size: 3010 1360
Fragments arranged by size:
3010 1704 1360 1262 792 622 534 92
71 11
BsaAI yAC'GTr
Cuts at: 165 4558 6811 165
Size: 4393 2253 2812
Fragments arranged by size:
4393 2812 2253
BsaBI GATnn'nnATC
Cuts at: 6 6972 9009 6
Size: 6966 2037 455
Fragments arranged by size:
6966 2037 455
BsaHI Gr'CG_yC
Cuts at: 2591 2979 5492 6240 6889 7137 7839 9335 2591
Size: 388 2513 748 649 248 702 1496 2714
Fragments arranged by size:
2714 2513 1496 748 702 649 388 248
BsaWI w'CCGG_w
Cuts at: 6647 7168 7846 8257 8404 8678 6647
Size: 521 678 411 147 274 7427
Fragments arranged by size:
7427 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1706 1736 1789 1819 2569 2599 3793 3823 1706
Size: 30 53 30 750 30 1194 30 7341
Fragments arranged by size:
7341 1194 750 53 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 663 826 958 1075 1159 1891 2281 2590 3112
Size: 163 132 117 84 732 390 309 522
Cuts at: 3112 3637 3814 3817 3919 3997 4018 4090 4270
Size: 525 177 3 102 78 21 72 180
Cuts at: 4270 4468 4684 5311 5652 5983 663
Size: 198 216 627 341 331 4138
Fragments arranged by size:
4138 732 627 525 522 390 341 331
309 216 198 180 177 163 132 117
102 84 78 72 21 3
BseYI C'CCAG_C
Cuts at: 1187 1292 1332 1706 1853 2030 2392 2492 4080
Size: 105 40 374 147 177 362 100 1588
Cuts at: 4080 4282 4313 4467 4865 4880 5070 5092 5255
Size: 202 31 154 398 15 190 22 163
Cuts at: 5255 5339 5387 6883 7641 8306 8714 1187
Size: 84 48 1496 758 665 408 1931
Fragments arranged by size:
1931 1588 1496 758 665 408 398 374
362 202 190 177 163 154 147 105
100 84 48 40 31 22 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 321 341 4009 4609 4828 119
Size: 202 20 3668 600 219 4749
Fragments arranged by size:
4749 3668 600 219 202 20
BsiEI CG_ry'CG
Cuts at: 104 7000 7046 8276 104
Size: 6896 46 1230 1286
Fragments arranged by size:
6896 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 436 611 1151 1205 1528 1628 2702 2738 2760
Size: 175 540 54 323 100 1074 36 22
Cuts at: 2760 3410 3597 4157 4609 4934 6187 6472 7250
Size: 650 187 560 452 325 1253 285 778
Cuts at: 7250 7440 8300 436
Size: 190 860 1594
Fragments arranged by size:
1594 1253 1074 860 778 650 560 540
452 325 323 285 190 187 175 100
54 36 22
BsiWI C'GTAC_G
Cuts at: 4554 4554
Size: 9458
BsmI GAATG_Cn'
Cuts at: 349 349
Size: 9458
BsmBI CGTCTCn'nnnn_
Cuts at: 750 5483 750
Size: 4733 4725
Bsp1286I G_dGCh'C
Cuts at: 436 611 932 1151 1205 1528 1590 1628 2010
Size: 175 321 219 54 323 62 38 382
Cuts at: 2010 2702 2738 2760 2793 3156 3410 3429 3597
Size: 692 36 22 33 363 254 19 168
Cuts at: 3597 3647 4157 4314 4609 4934 5243 5793 5824
Size: 50 510 157 295 325 309 550 31
Cuts at: 5824 5899 5956 6043 6089 6100 6187 6472 7083
Size: 75 57 87 46 11 87 285 611
Cuts at: 7083 7176 7250 7440 7502 8300 436
Size: 93 74 190 62 798 1594
Fragments arranged by size:
1594 798 692 611 550 510 382 363
325 323 321 309 295 285 254 219
190 175 168 157 93 87 87 75
74 62 62 57 54 50 46 38
36 33 31 22 19 11
BspHI T'CATG_A
Cuts at: 7890 7890
Size: 9458
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1526 2757 2875 4046 4746 5131 5923 6508 6722
Size: 1231 118 1171 700 385 792 585 214
Cuts at: 6722 7024 7405 1526
Size: 302 381 3579
Fragments arranged by size:
3579 1231 1171 792 700 585 385 381
302 214 118
BsrBI CCG'CTC
Cuts at: 50 1467 1902 2553 3021 3144 3567 3585 4476
Size: 1417 435 651 468 123 423 18 891
Cuts at: 4476 5522 5527 7750 7804 9288 9453 50
Size: 1046 5 2223 54 1484 165 55
Fragments arranged by size:
2223 1484 1417 1046 891 651 468 435
423 165 123 55 54 18 5
BsrDI GCAATG_nn'
Cuts at: 3048 3517 5864 6374 7370 3048
Size: 469 2347 510 996 5136
Fragments arranged by size:
5136 2347 996 510 469
BsrFI r'CCGG_y
Cuts at: 273 2711 3755 4490 6236 7456 7637 273
Size: 2438 1044 735 1746 1220 181 2094
Fragments arranged by size:
2438 2094 1746 1220 1044 735 181
BssSI C'ACGA_G
Cuts at: 7729 8437 7729
Size: 708 8750
BstAPI GCAn_nnn'nTGC
Cuts at: 4443 5167 5221 6835 4443
Size: 724 54 1614 7066
Fragments arranged by size:
7066 1614 724 54
BstBI TT'CG_AA
Cuts at: 1478 7819 1478
Size: 6341 3117
BstEII G'GTnAC_C
Cuts at: 2244 2244
Size: 9458
BstXI CCAn_nnnn'nTGG
Cuts at: 924 1582 6100 924
Size: 658 4518 4282
Fragments arranged by size:
4518 4282 658
BstYI r'GATC_y
Cuts at: 1 5283 6483 6950 6973 7308 7554 7860 7872
Size: 5282 1200 467 23 335 246 306 12
Cuts at: 7872 7958 9010 1
Size: 86 1052 449
Fragments arranged by size:
5282 1200 1052 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 237 719 2483 237
Size: 482 1764 7212
Fragments arranged by size:
7212 1764 482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7408 7589 7752 7408
Size: 181 163 9114
Fragments arranged by size:
9114 181 163
BtsI GCAGTG_nn'
Cuts at: 1006 1865 3478 4607 4706 6357 6401 6701 1006
Size: 859 1613 1129 99 1651 44 300 3763
Fragments arranged by size:
3763 1651 1613 1129 859 300 99 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3297 3332 3297
Size: 35 9423
DraI TTT'AAA
Cuts at: 5228 6457 5228
Size: 1229 8229
DraIII CAC_nnn'GTG
Cuts at: 1764 1764
Size: 9458
DrdI GACnn_nn'nnGTC
Cuts at: 7164 8508 7164
Size: 1344 8114
EaeI y'GGCC_r
Cuts at: 914 1862 2750 3182 3305 3875 4488 7043 7217
Size: 948 888 432 123 570 613 2555 174
Cuts at: 7217 7608 7635 8980 914
Size: 391 27 1345 1392
Fragments arranged by size:
2555 1392 1345 948 888 613 570 432
391 174 123 27
EagI C'GGCC_G
Cuts at: 7043 7043
Size: 9458
EarI CTCTTCn'nnn_
Cuts at: 242 339 599 681 1018 1232 1550 2258 2291
Size: 97 260 82 337 214 318 708 33
Cuts at: 2291 2378 2960 3883 4685 5579 7481 7691 242
Size: 87 582 923 802 894 1902 210 2009
Fragments arranged by size:
2009 1902 923 894 802 708 582 337
318 260 214 210 97 87 82 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1744 7666 8408 8554 9268 1744
Size: 5922 742 146 714 1934
Fragments arranged by size:
5922 1934 742 714 146
EcoICRI GAG'CTC
Cuts at: 434 609 1203 1526 1626 2700 2736 6470 434
Size: 175 594 323 100 1074 36 3734 3422
Fragments arranged by size:
3734 3422 1074 594 323 175 100 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 266 623 692 784 826 832 946 1015
Size: 85 357 69 92 42 6 114 69
Cuts at: 1015 1033 1213 1240 1444 1582 1699 1807 1945
Size: 18 180 27 204 138 117 108 138
Cuts at: 1945 1954 1963 1978 2056 2191 2203 2344 2428
Size: 9 9 15 78 135 12 141 84
Cuts at: 2428 2474 2686 2746 3193 3343 3367 3397 3493
Size: 46 212 60 447 150 24 30 96
Cuts at: 3493 3619 3743 3853 3922 4013 4282 4447 4642
Size: 126 124 110 69 91 269 165 195
Cuts at: 4642 4687 4843 4858 5041 5078 5119 5176 5384
Size: 45 156 15 183 37 41 57 208
Cuts at: 5384 5437 5485 5780 5929 5947 6002 6197 6240
Size: 53 48 295 149 18 55 195 43
Cuts at: 6240 7283 7715 8068 8730 181
Size: 1043 432 353 662 909
Fragments arranged by size:
1043 909 662 447 432 357 353 295
269 212 208 204 195 195 183 180
165 156 150 149 141 138 138 135
126 124 117 114 110 108 96 92
91 85 84 78 69 69 69 60
57 55 53 48 46 45 43 42
41 37 30 27 24 18 18 15
15 12 9 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 538 1543 1893 2263 3175 3253 3835 3922 4000
Size: 1005 350 370 912 78 582 87 78
Cuts at: 4000 4042 6328 538
Size: 42 2286 3668
Fragments arranged by size:
3668 2286 1005 912 582 370 350 87
78 78 42
EcoO109I rG'GnC_Cy
Cuts at: 543 1008 1586 1749 2223 2789 3463 3828 4131
Size: 465 578 163 474 566 674 365 303
Cuts at: 4131 4170 4662 5303 5457 5789 5790 6096 6097
Size: 39 492 641 154 332 1 306 1
Cuts at: 6097 6321 6597 543
Size: 224 276 3404
Fragments arranged by size:
3404 674 641 578 566 492 474 465
365 332 306 303 276 224 163 154
39 1 1
EcoRI G'AATT_C
Cuts at: 6462 6462
Size: 9458
EcoRV GAT'ATC
Cuts at: 729 729
Size: 9458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7477 7477
Size: 9458
FspI TGC'GCA
Cuts at: 2878 5149 7239 2878
Size: 2271 2090 5097
Fragments arranged by size:
5097 2271 2090
HaeII r_GCGC'y
Cuts at: 2594 3007 4872 5394 5600 6243 6398 6892 7140
Size: 413 1865 522 206 643 155 494 248
Cuts at: 7140 8370 9141 2594
Size: 1230 771 2911
Fragments arranged by size:
2911 1865 1230 771 643 522 494 413
248 206 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 245 277 2797 2829 3397 3429 3793 3825 5904
Size: 32 2520 32 568 32 364 32 2079
Cuts at: 5904 5936 7300 7330 7332 7362 245
Size: 32 1364 30 2 30 2341
Fragments arranged by size:
2520 2341 2079 1364 568 364 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1595 6497 1595
Size: 4902 4556
HindIII A'AGCT_T
Cuts at: 2797 3310 2797
Size: 513 8945
KasI G'GCGC_C
Cuts at: 2590 6239 6888 7136 2590
Size: 3649 649 248 4912
Fragments arranged by size:
4912 3649 649 248
KpnI G_GTAC'C
Cuts at: 6478 6478
Size: 9458
MluI A'CGCG_T
Cuts at: 8891 8891
Size: 9458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1559 1749 4493 8217 8401 1559
Size: 190 2744 3724 184 2616
Fragments arranged by size:
3724 2744 2616 190 184
MscI TGG'CCA
Cuts at: 1864 2752 3307 7219 1864
Size: 888 555 3912 4103
Fragments arranged by size:
4103 3912 888 555
MslI CAynn'nnrTG
Cuts at: 4970 5560 7447 7574 4970
Size: 590 1887 127 6854
Fragments arranged by size:
6854 1887 590 127
MspA1I CmG'CkG
Cuts at: 1191 1275 1296 1350 1497 1923 2034 2145 2503
Size: 84 21 54 147 426 111 111 358
Cuts at: 2503 2610 3387 4020 4215 4234 4282 4317 4383
Size: 107 777 633 195 19 48 35 66
Cuts at: 4383 4416 4680 4884 4927 5010 5028 5259 5847
Size: 33 264 204 43 83 18 231 588
Cuts at: 5847 6131 6569 6883 7243 8027 8272 1191
Size: 284 438 314 360 784 245 2377
Fragments arranged by size:
2377 784 777 633 588 438 426 360
358 314 284 264 245 231 204 195
147 111 111 107 84 83 66 54
48 43 35 33 21 19 18
NaeI GCC'GGC
Cuts at: 2713 3757 4492 7639 2713
Size: 1044 735 3147 4532
Fragments arranged by size:
4532 3147 1044 735
NarI GG'CG_CC
Cuts at: 2591 6240 6889 7137 2591
Size: 3649 649 248 4912
Fragments arranged by size:
4912 3649 649 248
NcoI C'CATG_G
Cuts at: 237 719 2483 237
Size: 482 1764 7212
Fragments arranged by size:
7212 1764 482
NgoMIV G'CCGG_C
Cuts at: 2711 3755 4490 7637 2711
Size: 1044 735 3147 4532
Fragments arranged by size:
4532 3147 1044 735
NheI G'CTAG_C
Cuts at: 816 1710 5644 816
Size: 894 3934 4630
Fragments arranged by size:
4630 3934 894
NspI r_CATG'y
Cuts at: 4425 6018 6511 7011 8614 4425
Size: 1593 493 500 1603 5269
Fragments arranged by size:
5269 1603 1593 500 493
PasI CC'CwG_GG
Cuts at: 446 1746 5484 446
Size: 1300 3738 4420
Fragments arranged by size:
4420 3738 1300
PciI A'CATG_T
Cuts at: 8610 8610
Size: 9458
PflMI CCAn_nnn'nTGG
Cuts at: 452 1764 1860 3303 3831 3900 4545 6249 452
Size: 1312 96 1443 528 69 645 1704 3661
Fragments arranged by size:
3661 1704 1443 1312 645 528 96 69
PfoI T'CCnGG_A
Cuts at: 197 2207 2600 3830 3998 4664 4685 5498 5796
Size: 2010 393 1230 168 666 21 813 298
Cuts at: 5796 197
Size: 3859
Fragments arranged by size:
3859 2010 1230 813 666 393 298 168
21
PmeI GTTT'AAAC
Cuts at: 6457 6457
Size: 9458
PmlI CAC'GTG
Cuts at: 165 165
Size: 9458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 7904 7936 3539
Size: 32 4333 32 5061
Fragments arranged by size:
5061 4333 32 32
PpuMI rG'GwC_Cy
Cuts at: 543 1008 3828 4662 5303 543
Size: 465 2820 834 641 4698
Fragments arranged by size:
4698 2820 834 641 465
PshAI GACnn'nnGTC
Cuts at: 2455 5507 2455
Size: 3052 6406
PspOMI G'GGCC_C
Cuts at: 1586 2789 4310 5789 6096 1586
Size: 1203 1521 1479 307 4948
Fragments arranged by size:
4948 1521 1479 1203 307
PspXI vC'TCGA_Gb
Cuts at: 3556 4150 6428 3556
Size: 594 2278 6586
Fragments arranged by size:
6586 2278 594
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5797 5829 5797
Size: 32 9426
PstI C_TGCA'G
Cuts at: 194 208 314 737 1112 1220 1301 1523 1875
Size: 14 106 423 375 108 81 222 352
Cuts at: 1875 1970 2286 2657 2825 2992 3011 3212 3611
Size: 95 316 371 168 167 19 201 399
Cuts at: 3611 3926 3960 4148 4220 4946 5003 5033 5879
Size: 315 34 188 72 726 57 30 846
Cuts at: 5879 6505 7190 194
Size: 626 685 2462
Fragments arranged by size:
2462 846 726 685 626 423 399 375
371 352 316 315 222 201 188 168
167 108 106 95 81 72 57 34
30 19 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9458
PvuII CAG'CTG
Cuts at: 1191 1275 1296 1350 1497 1923 2145 2503 2610
Size: 84 21 54 147 426 222 358 107
Cuts at: 2610 3387 4215 4383 4416 4884 4927 5010 5028
Size: 777 828 168 33 468 43 83 18
Cuts at: 5028 5259 5847 6131 6883 7243 1191
Size: 231 588 284 752 360 3406
Fragments arranged by size:
3406 828 777 752 588 468 426 360
358 284 231 222 168 147 107 84
83 54 43 33 21 18
RsrII CG'GwC_CG
Cuts at: 7653 7653
Size: 9458
SacI G_AGCT'C
Cuts at: 436 611 1205 1528 1628 2702 2738 6472 436
Size: 175 594 323 100 1074 36 3734 3422
Fragments arranged by size:
3734 3422 1074 594 323 175 100 36
SalI G'TCGA_C
Cuts at: 6495 6495
Size: 9458
SanDI GG'GwC_CC
Cuts at: 5303 5303
Size: 9458
SapI GCTCTTCn'nnn_
Cuts at: 599 1018 1550 2291 2960 3883 5579 7481 7691
Size: 419 532 741 669 923 1696 1902 210
Cuts at: 7691 599
Size: 2366
Fragments arranged by size:
2366 1902 1696 923 741 669 532 419
210
SbfI CC_TGCA'GG
Cuts at: 1220 6505 1220
Size: 5285 4173
ScaI AGT'ACT
Cuts at: 8975 8975
Size: 9458
SexAI A'CCwGG_T
Cuts at: 1760 1760
Size: 9458
SfcI C'TryA_G
Cuts at: 33 190 204 310 559 658 733 1108 1117
Size: 157 14 106 249 99 75 375 9
Cuts at: 1117 1216 1297 1519 1871 1966 2282 2653 2821
Size: 99 81 222 352 95 316 371 168
Cuts at: 2821 2988 3007 3208 3607 3922 3956 4144 4216
Size: 167 19 201 399 315 34 188 72
Cuts at: 4216 4942 4999 5029 5083 5613 5875 6501 7186
Size: 726 57 30 54 530 262 626 685
Cuts at: 7186 8154 8345 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 726 685 626 530 399 375
371 352 316 315 262 249 222 201
191 188 168 167 157 106 99 99
95 81 75 72 57 54 34 30
19 14 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3745 4228 3745
Size: 483 8975
SfoI GGC'GCC
Cuts at: 2592 6241 6890 7138 2592
Size: 3649 649 248 4912
Fragments arranged by size:
4912 3649 649 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9458
SmaI CCC'GGG
Cuts at: 127 1999 2911 3577 3751 4525 4615 6480 127
Size: 1872 912 666 174 774 90 1865 3105
Fragments arranged by size:
3105 1872 1865 912 774 666 174 90
SmlI C'TyrA_G
Cuts at: 940 1893 1915 3196 3556 4150 5265 6428 6613
Size: 953 22 1281 360 594 1115 1163 185
Cuts at: 6613 7528 8242 8504 940
Size: 915 714 262 1894
Fragments arranged by size:
1894 1281 1163 1115 953 915 714 594
360 262 185 22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9458
SphI G_CATG'C
Cuts at: 4425 6018 6511 7011 4425
Size: 1593 493 500 6872
Fragments arranged by size:
6872 1593 500 493
StuI AGG'CCT
Cuts at: 1570 1741 1888 2077 5937 1570
Size: 171 147 189 3860 5091
Fragments arranged by size:
5091 3860 189 171 147
StyI C'CwwG_G
Cuts at: 237 719 932 1550 2249 2483 2870 3698 4485
Size: 482 213 618 699 234 387 828 787
Cuts at: 4485 4652 5105 5402 6329 6434 6592 6946 237
Size: 167 453 297 927 105 158 354 2749
Fragments arranged by size:
2749 927 828 787 699 618 482 453
387 354 297 234 213 167 158 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 138 712 1324 4459 6176 6288 7041 7174 7633
Size: 574 612 3135 1717 112 753 133 459
Cuts at: 7633 7842 138
Size: 209 1754
Fragments arranged by size:
3135 1754 1717 753 612 574 459 209
133 112
TatI w'GTAC_w
Cuts at: 8973 8973
Size: 9458
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 283 315 1706 1738 8975 9007 283
Size: 32 1391 32 7237 32 734
Fragments arranged by size:
7237 1391 734 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7255 7255
Size: 9458
XbaI T'CTAG_A
Cuts at: 6489 6489
Size: 9458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1557 2850 4321 6097 6953 1557
Size: 1293 1471 1776 856 4062
Fragments arranged by size:
4062 1776 1471 1293 856
XhoI C'TCGA_G
Cuts at: 3556 4150 6428 3556
Size: 594 2278 6586
Fragments arranged by size:
6586 2278 594
XmaI C'CCGG_G
Cuts at: 125 1997 2909 3575 3749 4523 4613 6478 125
Size: 1872 912 666 174 774 90 1865 3105
Fragments arranged by size:
3105 1872 1865 912 774 666 174 90
XmnI GAAnn'nnTTC
Cuts at: 1253 1266 4114 5809 1253
Size: 13 2848 1695 4902
Fragments arranged by size:
4902 2848 1695 13
ZraI GAC'GTC
Cuts at: 5493 5493
Size: 9458
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AhdI AleI AloI AscI AvrII BaeI BfrBI
BmgBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI FseI
FspAI HpaI MfeI NdeI NotI NruI NsiI PacI
PsiI SacII SgrAI SnaBI SrfI SspI SwaI