Restriction map for
pF1KB0019
(Circular) (Six-base) MAPSORT of: pF1KB0019.seq Check: 2217 from: 1 to: 11642
pF1KB0019 11642 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 8061 8061
Size: 11642
AccI GT'mk_AC
Cuts at: 798 2091 2830 7062 7218 8680 798
Size: 1293 739 4232 156 1462 3760
Fragments arranged by size:
4232 3760 1462 1293 739 156
Acc65I G'GTAC_C
Cuts at: 721 8658 721
Size: 7937 3705
AclI AA'CG_TT
Cuts at: 3543 11482 3543
Size: 7939 3703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 577 3089 3572 3977 4076 4121 4157 5872
Size: 402 2512 483 405 99 45 36 1715
Cuts at: 5872 6230 8486 9467 9899 10646 10914 175
Size: 358 2256 981 432 747 268 903
Fragments arranged by size:
2512 2256 1715 981 903 747 483 432
405 402 358 268 99 45 36
AflII C'TTAA_G
Cuts at: 6865 7461 6865
Size: 596 11046
AflIII A'CryG_T
Cuts at: 2944 5467 5469 7878 8994 10098 11075 2944
Size: 2523 2 2409 1116 1104 977 3511
Fragments arranged by size:
3511 2523 2409 1116 1104 977 2
AgeI A'CCGG_T
Cuts at: 10030 10030
Size: 11642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1514 1754 5384 1514
Size: 240 3630 7772
Fragments arranged by size:
7772 3630 240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8580 8612 8580
Size: 32 11610
AlwNI CAG_nnn'CTG
Cuts at: 1429 4000 4561 5947 7189 8947 10514 1429
Size: 2571 561 1386 1242 1758 1567 2557
Fragments arranged by size:
2571 2557 1758 1567 1386 1242 561
ApaLI G'TGCA_C
Cuts at: 1940 10412 1940
Size: 8472 3170
ApoI r'AATT_y
Cuts at: 636 767 1221 1755 1887 2931 4165 5155 5550
Size: 131 454 534 132 1044 1234 990 395
Cuts at: 5550 5964 6399 6432 6605 6731 7963 8646 10995
Size: 414 435 33 173 126 1232 683 2349
Cuts at: 10995 11251 636
Size: 256 1027
Fragments arranged by size:
2349 1234 1232 1044 1027 990 683 534
454 435 414 395 256 173 132 131
126 33
AseI AT'TA_AT
Cuts at: 20 3470 5291 20
Size: 3450 1821 6371
Fragments arranged by size:
6371 3450 1821
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11642
AvaI C'yCGr_G
Cuts at: 1226 1244 6782 7784 8662 1226
Size: 18 5538 1002 878 4206
Fragments arranged by size:
5538 4206 1002 878 18
AvrII C'CTAG_G
Cuts at: 8476 8476
Size: 11642
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6146 6179 6146
Size: 33 11609
BamHI G'GATC_C
Cuts at: 8667 8667
Size: 11642
BanI G'GyrC_C
Cuts at: 721 8049 8658 9072 9320 9355 721
Size: 7328 609 414 248 35 3008
Fragments arranged by size:
7328 3008 609 414 248 35
BanII G_rGCy'C
Cuts at: 6083 8656 9686 6083
Size: 2573 1030 8039
Fragments arranged by size:
8039 2573 1030
BbeI G_GCGC'C
Cuts at: 9076 9324 9076
Size: 248 11394
BbsI GAAGACnn'nnnn_
Cuts at: 613 1156 1727 2819 3734 5366 5565 7358 7674
Size: 543 571 1092 915 1632 199 1793 316
Cuts at: 7674 613
Size: 4581
Fragments arranged by size:
4581 1793 1632 1092 915 571 543 316
199
BciVI GTATCCnnnnn_n'
Cuts at: 7935 9540 10301 11642 7935
Size: 1605 761 1341 7935
Fragments arranged by size:
7935 1605 1341 761
BclI T'GATC_A
Cuts at: 126 6564 9162 126
Size: 6438 2598 2606
Fragments arranged by size:
6438 2606 2598
BfrBI ATG'CAT
Cuts at: 2146 5288 2146
Size: 3142 8500
BglI GCCn_nnn'nGGC
Cuts at: 1654 1654
Size: 11642
BglII A'GATC_T
Cuts at: 1442 2744 2980 6330 8116 8125 9157 11194 1442
Size: 1302 236 3350 1786 9 1032 2037 1890
Fragments arranged by size:
3350 2037 1890 1786 1302 1032 236 9
BlpI GC'TnA_GC
Cuts at: 878 2405 8754 878
Size: 1527 6349 3766
Fragments arranged by size:
6349 3766 1527
Bme1580I G_kGCm'C
Cuts at: 1944 5145 9267 9360 10416 1944
Size: 3201 4122 93 1056 3170
Fragments arranged by size:
4122 3201 3170 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 4361 5904 6154 8854 8935 9270 11414 4361
Size: 1543 250 2700 81 335 2144 4589
Fragments arranged by size:
4589 2700 2144 1543 335 250 81
BmtI G_CTAG'C
Cuts at: 1114 1114
Size: 11642
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 860 7314 10009 860
Size: 6454 2695 2493
Fragments arranged by size:
6454 2695 2493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 449 4580 4670 5141 6857 7196 7535 8513
Size: 72 4131 90 471 1716 339 339 978
Cuts at: 8513 377
Size: 3506
Fragments arranged by size:
4131 3506 1716 978 471 339 339 90
72
Bpu10I CC'TnA_GC
Cuts at: 4508 8600 4508
Size: 4092 7550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 2212 2331 3352 5192 6098 6156 6259 6813
Size: 1674 119 1021 1840 906 58 103 554
Cuts at: 6813 8391 8818 9697 10189 10487 538
Size: 1578 427 879 492 298 1693
Fragments arranged by size:
1840 1693 1674 1578 1021 906 879 554
492 427 298 119 103 58
BsaI GGTCTCn'nnnn_
Cuts at: 3573 4237 6354 7703 11346 3573
Size: 664 2117 1349 3643 3869
Fragments arranged by size:
3869 3643 2117 1349 664
BsaAI yAC'GTr
Cuts at: 4873 5470 8995 4873
Size: 597 3525 7520
Fragments arranged by size:
7520 3525 597
BsaBI GATnn'nnATC
Cuts at: 6 414 7014 9156 11193 6
Size: 408 6600 2142 2037 455
Fragments arranged by size:
6600 2142 2037 455 408
BsaHI Gr'CG_yC
Cuts at: 9073 9321 10023 11519 9073
Size: 248 702 1496 9196
Fragments arranged by size:
9196 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1973 5985 8831 9352 10030 10304 10451 10862 1973
Size: 4012 2846 521 678 274 147 411 2753
Fragments arranged by size:
4012 2846 2753 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 5114 5144 6900 6930 5114
Size: 30 1756 30 9826
Fragments arranged by size:
9826 1756 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1162 2598 4424 5150 6816 6938 1162
Size: 1436 1826 726 1666 122 5866
Fragments arranged by size:
5866 1826 1666 1436 726 122
BseYI C'CCAG_C
Cuts at: 2602 3108 7046 9067 9825 10402 10898 2602
Size: 506 3938 2021 758 577 496 3346
Fragments arranged by size:
3938 3346 2021 758 577 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 989 1280 3797 5366 456
Size: 533 291 2517 1569 6732
Fragments arranged by size:
6732 2517 1569 533 291
BsiEI CG_ry'CG
Cuts at: 104 9184 9230 10438 104
Size: 9080 46 1208 1308
Fragments arranged by size:
9080 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 750 1692 1944 3352 4206 4632 7760 8656 9434
Size: 942 252 1408 854 426 3128 896 778
Cuts at: 9434 9624 10416 750
Size: 190 792 1976
Fragments arranged by size:
3128 1976 1408 942 896 854 792 778
426 252 190
BsmI GAATG_Cn'
Cuts at: 5290 7272 5290
Size: 1982 9660
Bsp1286I G_dGCh'C
Cuts at: 750 1692 1944 3352 4206 4632 5145 6083 7760
Size: 942 252 1408 854 426 513 938 1677
Cuts at: 7760 8656 9267 9360 9434 9624 9686 10416 750
Size: 896 611 93 74 190 62 730 1976
Fragments arranged by size:
1976 1677 1408 942 938 896 854 730
611 513 426 252 190 93 74 62
BspEI T'CCGG_A
Cuts at: 10862 10862
Size: 11642
BspHI T'CATG_A
Cuts at: 1080 1386 10818 1080
Size: 306 9432 1904
Fragments arranged by size:
9432 1904 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 733 1722 7050 8061 8692 8906 9208 9589 733
Size: 989 5328 1011 631 214 302 381 2786
Fragments arranged by size:
5328 2786 1011 989 631 381 302 214
BsrBI CCG'CTC
Cuts at: 50 882 9934 9988 11472 11637 50
Size: 832 9052 54 1484 165 55
Fragments arranged by size:
9052 1484 832 165 55 54
BsrDI GCAATG_nn'
Cuts at: 532 1200 1508 1529 1899 6251 9554 532
Size: 668 308 21 370 4352 3303 2620
Fragments arranged by size:
4352 3303 2620 668 370 308 21
BsrFI r'CCGG_y
Cuts at: 862 9640 9821 10030 862
Size: 8778 181 209 2474
Fragments arranged by size:
8778 2474 209 181
BsrGI T'GTAC_A
Cuts at: 4949 4949
Size: 11642
BssSI C'ACGA_G
Cuts at: 9913 10271 9913
Size: 358 11284
BstAPI GCAn_nnn'nTGC
Cuts at: 4000 4483 4525 9019 4000
Size: 483 42 4494 6623
Fragments arranged by size:
6623 4494 483 42
BstBI TT'CG_AA
Cuts at: 6816 10003 6816
Size: 3187 8455
BstXI CCAn_nnnn'nTGG
Cuts at: 2778 6309 6985 2778
Size: 3531 676 7435
Fragments arranged by size:
7435 3531 676
BstYI r'GATC_y
Cuts at: 1 409 1442 2744 2980 4059 6330 7015 8116
Size: 408 1033 1302 236 1079 2271 685 1101
Cuts at: 8116 8125 8519 8667 9134 9157 9492 9738 10750
Size: 9 394 148 467 23 335 246 1012
Cuts at: 10750 10836 10848 11194 1
Size: 86 12 346 449
Fragments arranged by size:
2271 1302 1101 1079 1033 1012 685 467
449 408 394 346 335 246 236 148
86 23 12 9
BstZ17I GTA'TAC
Cuts at: 799 799
Size: 11642
Bsu36I CC'TnA_GG
Cuts at: 7182 8001 7182
Size: 819 10823
BtgI C'CryG_G
Cuts at: 107 4583 5023 8252 107
Size: 4476 440 3229 3497
Fragments arranged by size:
4476 3497 3229 440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 718 9592 9773 9936 718
Size: 8874 181 163 2424
Fragments arranged by size:
8874 2424 181 163
BtsI GCAGTG_nn'
Cuts at: 2753 3756 4088 4344 8241 8885 2753
Size: 1003 332 256 3897 644 5510
Fragments arranged by size:
5510 3897 1003 644 332 256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3431 3466 3431
Size: 35 11607
DraI TTT'AAA
Cuts at: 2999 3650 5504 8641 2999
Size: 651 1854 3137 6000
Fragments arranged by size:
6000 3137 1854 651
DraIII CAC_nnn'GTG
Cuts at: 8428 8428
Size: 11642
DrdI GACnn_nn'nnGTC
Cuts at: 9348 10206 9348
Size: 858 10784
EaeI y'GGCC_r
Cuts at: 7080 8509 9227 9401 9792 9819 11164 7080
Size: 1429 718 174 391 27 1345 7558
Fragments arranged by size:
7558 1429 1345 718 391 174 27
EagI C'GGCC_G
Cuts at: 9227 9227
Size: 11642
EarI CTCTTCn'nnn_
Cuts at: 462 1481 2366 2616 3569 3605 9665 9875 462
Size: 1019 885 250 953 36 6060 210 2229
Fragments arranged by size:
6060 2229 1019 953 885 250 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9850 10160 10306 11452 9850
Size: 310 146 1146 10040
Fragments arranged by size:
10040 1146 310 146
EcoICRI GAG'CTC
Cuts at: 8654 8654
Size: 11642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 377 449 577 3089 3572 3977 4076 4121
Size: 202 72 128 2512 483 405 99 45
Cuts at: 4121 4157 4580 4670 5141 5872 6230 6857 7196
Size: 36 423 90 471 731 358 627 339
Cuts at: 7196 7535 8486 8513 9467 9899 10646 10914 175
Size: 339 951 27 954 432 747 268 903
Fragments arranged by size:
2512 954 951 903 747 731 627 483
471 432 423 405 358 339 339 268
202 128 99 90 72 45 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 5183 5654 6223 6893 5183
Size: 471 569 670 9932
Fragments arranged by size:
9932 670 569 471
EcoO109I rG'GnC_Cy
Cuts at: 4791 7535 8781 4791
Size: 2744 1246 7652
Fragments arranged by size:
7652 2744 1246
EcoRI G'AATT_C
Cuts at: 767 6605 8646 767
Size: 5838 2041 3763
Fragments arranged by size:
5838 3763 2041
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3344 3506 5639 7835 9661 3344
Size: 162 2133 2196 1826 5325
Fragments arranged by size:
5325 2196 2133 1826 162
FspI TGC'GCA
Cuts at: 9423 9423
Size: 11642
HaeII r_GCGC'y
Cuts at: 7683 8607 9076 9324 10346 11325 7683
Size: 924 469 248 1022 979 8000
Fragments arranged by size:
8000 1022 979 924 469 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 1011 1043 1081 1113 1189 1221 2521
Size: 32 153 32 38 32 76 32 1300
Cuts at: 2521 2553 3745 3777 3809 7712 7744 8511 8543
Size: 32 1192 32 32 3903 32 767 32
Cuts at: 8543 9484 9514 9516 9546 826
Size: 941 30 2 30 2922
Fragments arranged by size:
3903 2922 1300 1192 941 767 153 76
38 32 32 32 32 32 32 32
32 32 30 30 2
HincII GTy'rAC
Cuts at: 5724 7430 8681 5724
Size: 1706 1251 8685
Fragments arranged by size:
8685 1706 1251
HindIII A'AGCT_T
Cuts at: 2349 2625 6488 2349
Size: 276 3863 7503
Fragments arranged by size:
7503 3863 276
KasI G'GCGC_C
Cuts at: 9072 9320 9072
Size: 248 11394
KpnI G_GTAC'C
Cuts at: 725 8662 725
Size: 7937 3705
MfeI C'AATT_G
Cuts at: 670 670
Size: 11642
MluI A'CGCG_T
Cuts at: 11075 11075
Size: 11642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1785 2906 3399 5837 10313 10497 1785
Size: 1121 493 2438 4476 184 2930
Fragments arranged by size:
4476 2930 2438 1121 493 184
MscI TGG'CCA
Cuts at: 9403 9403
Size: 11642
MslI CAynn'nnrTG
Cuts at: 1193 1496 3149 9631 9758 1193
Size: 303 1653 6482 127 3077
Fragments arranged by size:
6482 3077 1653 303 127
MspA1I CmG'CkG
Cuts at: 1136 3955 4054 4941 8753 9067 9427 10440 10685
Size: 2819 99 887 3812 314 360 1013 245
Cuts at: 10685 1136
Size: 2093
Fragments arranged by size:
3812 2819 2093 1013 887 360 314 245
99
NaeI GCC'GGC
Cuts at: 9823 9823
Size: 11642
NarI GG'CG_CC
Cuts at: 9073 9321 9073
Size: 248 11394
NcoI C'CATG_G
Cuts at: 107 107
Size: 11642
NdeI CA'TA_TG
Cuts at: 123 3146 3737 123
Size: 3023 591 8028
Fragments arranged by size:
8028 3023 591
NgoMIV G'CCGG_C
Cuts at: 9821 9821
Size: 11642
NheI G'CTAG_C
Cuts at: 1110 1110
Size: 11642
NruI TCG'CGA
Cuts at: 1101 1101
Size: 11642
NsiI A_TGCA'T
Cuts at: 2148 5290 2148
Size: 3142 8500
NspI r_CATG'y
Cuts at: 1501 2150 2226 2948 4996 6476 6771 7882 8695
Size: 649 76 722 2048 1480 295 1111 813
Cuts at: 8695 9195 10102 1501
Size: 500 907 3041
Fragments arranged by size:
3041 2048 1480 1111 907 813 722 649
500 295 76
PciI A'CATG_T
Cuts at: 2944 7878 10098 2944
Size: 4934 2220 4488
Fragments arranged by size:
4934 4488 2220
PflMI CCAn_nnn'nTGG
Cuts at: 735 3385 4339 735
Size: 2650 954 8038
Fragments arranged by size:
8038 2650 954
PfoI T'CCnGG_A
Cuts at: 2715 6807 2715
Size: 4092 7550
PmeI GTTT'AAAC
Cuts at: 8641 8641
Size: 11642
PmlI CAC'GTG
Cuts at: 5470 5470
Size: 11642
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10781 10813 10781
Size: 32 11610
PpuMI rG'GwC_Cy
Cuts at: 4791 7535 4791
Size: 2744 8898
PshAI GACnn'nnGTC
Cuts at: 2134 2134
Size: 11642
PsiI TTA'TAA
Cuts at: 4360 6692 4360
Size: 2332 9310
PspXI vC'TCGA_Gb
Cuts at: 7784 7784
Size: 11642
PstI C_TGCA'G
Cuts at: 117 2280 2760 4121 4521 4533 8689 9374 117
Size: 2163 480 1361 400 12 4156 685 2385
Fragments arranged by size:
4156 2385 2163 1361 685 480 400 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11642
PvuII CAG'CTG
Cuts at: 1136 3955 4054 4941 9067 9427 1136
Size: 2819 99 887 4126 360 3351
Fragments arranged by size:
4126 3351 2819 887 360 99
RsrII CG'GwC_CG
Cuts at: 9837 9837
Size: 11642
SacI G_AGCT'C
Cuts at: 8656 8656
Size: 11642
SalI G'TCGA_C
Cuts at: 8679 8679
Size: 11642
SapI GCTCTTCn'nnn_
Cuts at: 1481 9665 9875 1481
Size: 8184 210 3248
Fragments arranged by size:
8184 3248 210
SbfI CC_TGCA'GG
Cuts at: 8689 8689
Size: 11642
ScaI AGT'ACT
Cuts at: 248 498 4593 7164 7983 11159 248
Size: 250 4095 2571 819 3176 731
Fragments arranged by size:
4095 3176 2571 819 731 250
SexAI A'CCwGG_T
Cuts at: 5351 5351
Size: 11642
SfcI C'TryA_G
Cuts at: 33 113 1522 1584 1931 2276 2591 2756 4117
Size: 80 1409 62 347 345 315 165 1361
Cuts at: 4117 4517 4529 5180 7649 8372 8685 9370 10363
Size: 400 12 651 2469 723 313 685 993
Cuts at: 10363 10554 33
Size: 191 1121
Fragments arranged by size:
2469 1409 1361 1121 993 723 685 651
400 347 345 315 313 191 165 80
62 12
SfoI GGC'GCC
Cuts at: 9074 9322 9074
Size: 248 11394
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11642
SmaI CCC'GGG
Cuts at: 1228 8664 1228
Size: 7436 4206
SmlI C'TyrA_G
Cuts at: 517 2227 2346 3367 5207 6113 6171 6274 6782
Size: 1710 119 1021 1840 906 58 103 508
Cuts at: 6782 6828 6865 7461 7784 8406 8797 9712 10204
Size: 46 37 596 323 622 391 915 492
Cuts at: 10204 10466 517
Size: 262 1693
Fragments arranged by size:
1840 1710 1693 1021 915 906 622 596
508 492 391 323 262 119 103 58
46 37
SpeI A'CTAG_T
Cuts at: 67 67
Size: 11642
SphI G_CATG'C
Cuts at: 2226 8695 9195 2226
Size: 6469 500 4673
Fragments arranged by size:
6469 4673 500
SspI AAT'ATT
Cuts at: 2331 3199 2331
Size: 868 10774
StuI AGG'CCT
Cuts at: 2307 2307
Size: 11642
StyI C'CwwG_G
Cuts at: 107 1037 2450 5567 7144 8476 8776 9130 107
Size: 930 1413 3117 1577 1332 300 354 2619
Fragments arranged by size:
3117 2619 1577 1413 1332 930 354 300
SwaI ATTT'AAAT
Cuts at: 5504 5504
Size: 11642
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4227 5400 6301 8070 9225 9358 9817 10026 4227
Size: 1173 901 1769 1155 133 459 209 5843
Fragments arranged by size:
5843 1769 1173 1155 901 459 209 133
TatI w'GTAC_w
Cuts at: 246 496 2315 3322 4267 4591 4949 5194 6628
Size: 250 1819 1007 945 324 358 245 1434
Cuts at: 6628 6832 7162 7981 11157 246
Size: 204 330 819 3176 731
Fragments arranged by size:
3176 1819 1434 1007 945 819 731 358
330 324 250 245 204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1025 1057 3651 3683 6900 6932 11159 11191 1025
Size: 32 2594 32 3217 32 4227 32 1476
Fragments arranged by size:
4227 3217 2594 1476 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5353 9439 5353
Size: 4086 7556
XbaI T'CTAG_A
Cuts at: 5700 6481 7006 7018 7357 7513 7717 8673 5700
Size: 781 525 12 339 156 204 956 8669
Fragments arranged by size:
8669 956 781 525 339 204 156 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 669 1044 1186 3382 5241 8250 9137 669
Size: 375 142 2196 1859 3009 887 3174
Fragments arranged by size:
3174 3009 2196 1859 887 375 142
XhoI C'TCGA_G
Cuts at: 6782 7784 6782
Size: 1002 10640
XmaI C'CCGG_G
Cuts at: 1226 8662 1226
Size: 7436 4206
XmnI GAAnn'nnTTC
Cuts at: 2724 5685 6180 7365 2724
Size: 2961 495 1185 7001
Fragments arranged by size:
7001 2961 1185 495
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI ApaI AscI BbvCI BcgI
BmgBI BsiWI BsmBI BssHII BstEII ClaI EcoRV FseI
FspAI HpaI NotI PacI PasI PspOMI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI ZraI