Restriction map for
pF1KB0015
(Circular) (Six-base) MAPSORT of: pF1KB0015.seq Check: 5470 from: 1 to: 7886
pF1KB0015 7886 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3267 3720 3267
Size: 453 7433
AatII G_ACGT'C
Cuts at: 1823 2222 1823
Size: 399 7487
AccI GT'mk_AC
Cuts at: 2335 4924 2335
Size: 2589 5297
Acc65I G'GTAC_C
Cuts at: 4902 4902
Size: 7886
AclI AA'CG_TT
Cuts at: 7726 7726
Size: 7886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 2426 2752 2799 2974 3739 3994 4006 4246
Size: 2053 326 47 175 765 255 12 240
Cuts at: 4246 5711 6143 6496 7158 373
Size: 1465 432 353 662 1101
Fragments arranged by size:
2053 1465 1101 765 662 432 353 326
255 240 175 47 12
AflII C'TTAA_G
Cuts at: 1459 1459
Size: 7886
AflIII A'CryG_T
Cuts at: 1484 2338 2543 5238 7038 7319 1484
Size: 854 205 2695 1800 281 2051
Fragments arranged by size:
2695 2051 1800 854 281 205
AhdI GACnn_n'nnGTC
Cuts at: 362 2127 3573 362
Size: 1765 1446 4675
Fragments arranged by size:
4675 1765 1446
AleI CACnn'nnGTG
Cuts at: 175 328 4520 175
Size: 153 4192 3541
Fragments arranged by size:
4192 3541 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 569 1715 2113 3155 569
Size: 1146 398 1042 5300
Fragments arranged by size:
5300 1146 1042 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2041 2073 2041
Size: 32 7854
AlwNI CAG_nnn'CTG
Cuts at: 783 1671 3645 5191 6629 783
Size: 888 1974 1546 1438 2040
Fragments arranged by size:
2040 1974 1546 1438 888
ApaI G_GGCC'C
Cuts at: 2987 3369 4572 4622 2987
Size: 382 1203 50 6251
Fragments arranged by size:
6251 1203 382 50
ApaLI G'TGCA_C
Cuts at: 745 1527 6724 745
Size: 782 5197 1907
Fragments arranged by size:
5197 1907 782
ApoI r'AATT_y
Cuts at: 142 163 467 2470 3057 3352 4834 4890 7239
Size: 21 304 2003 587 295 1482 56 2349
Cuts at: 7239 7495 142
Size: 256 533
Fragments arranged by size:
2349 2003 1482 587 533 304 295 256
56 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 7886
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7886
AvaI C'yCGr_G
Cuts at: 803 2411 4621 4667 4829 4906 803
Size: 1608 2210 46 162 77 3783
Fragments arranged by size:
3783 2210 1608 162 77 46
AvrII C'CTAG_G
Cuts at: 1167 1167
Size: 7886
BamHI G'GATC_C
Cuts at: 1235 1963 4911 1235
Size: 728 2948 4210
Fragments arranged by size:
4210 2948 728
BanI G'GyrC_C
Cuts at: 1888 3279 4060 4742 4902 5316 5564 5599 1888
Size: 1391 781 682 160 414 248 35 4175
Fragments arranged by size:
4175 1391 781 682 414 248 160 35
BanII G_rGCy'C
Cuts at: 1337 2987 3369 3761 3995 4252 4572 4622 4900
Size: 1650 382 392 234 257 320 50 278
Cuts at: 4900 5930 1337
Size: 1030 3293
Fragments arranged by size:
3293 1650 1030 392 382 320 278 257
234 50
BbeI G_GCGC'C
Cuts at: 5320 5568 5320
Size: 248 7638
BbsI GAAGACnn'nnnn_
Cuts at: 361 647 1815 3435 3970 361
Size: 286 1168 1620 535 4277
Fragments arranged by size:
4277 1620 1168 535 286
BbvCI CC'TCA_GC
Cuts at: 1727 3876 1727
Size: 2149 5737
BciVI GTATCCnnnnn_n'
Cuts at: 3354 5784 6840 7886 3354
Size: 2430 1056 1046 3354
Fragments arranged by size:
3354 2430 1056 1046
BclI T'GATC_A
Cuts at: 228 5406 228
Size: 5178 2708
BfrBI ATG'CAT
Cuts at: 1201 3160 3856 1201
Size: 1959 696 5231
Fragments arranged by size:
5231 1959 696
BglI GCCn_nnn'nGGC
Cuts at: 1308 3880 4751 1308
Size: 2572 871 4443
Fragments arranged by size:
4443 2572 871
BglII A'GATC_T
Cuts at: 434 1140 1799 4254 5401 7438 434
Size: 706 659 2455 1147 2037 882
Fragments arranged by size:
2455 2037 1147 882 706 659
BlpI GC'TnA_GC
Cuts at: 3760 4093 4998 3760
Size: 333 905 6648
Fragments arranged by size:
6648 905 333
Bme1580I G_kGCm'C
Cuts at: 749 1531 1935 2987 3369 3847 4063 4572 4622
Size: 782 404 1052 382 478 216 509 50
Cuts at: 4622 4747 5511 5604 6728 749
Size: 125 764 93 1124 1907
Fragments arranged by size:
1907 1124 1052 782 764 509 478 404
382 216 125 93 50
BmrI ACTGGGnnnn_n'
Cuts at: 621 4421 4575 5098 5179 5514 7658 621
Size: 3800 154 523 81 335 2144 849
Fragments arranged by size:
3800 2144 849 523 335 154 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 996 6253 996
Size: 5257 2629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2954 3253 3679 3845 4261 1246
Size: 1708 299 426 166 416 4871
Fragments arranged by size:
4871 1708 426 416 299 166
Bpu10I CC'TnA_GC
Cuts at: 1727 3876 3884 1727
Size: 2149 8 5729
Fragments arranged by size:
5729 2149 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4441 5062 5941 6655 6953 4096
Size: 345 621 879 714 298 5029
Fragments arranged by size:
5029 879 714 621 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 682 7590 682
Size: 6908 978
BsaAI yAC'GTr
Cuts at: 1485 1980 2328 3249 5239 1485
Size: 495 348 921 1990 4132
Fragments arranged by size:
4132 1990 921 495 348
BsaBI GATnn'nnATC
Cuts at: 6 5400 7437 6
Size: 5394 2037 455
Fragments arranged by size:
5394 2037 455
BsaHI Gr'CG_yC
Cuts at: 1820 2219 3866 5317 5565 6267 7763 1820
Size: 399 1647 1451 248 702 1496 1943
Fragments arranged by size:
1943 1647 1496 1451 702 399 248
BsaWI w'CCGG_w
Cuts at: 1733 3476 4793 5075 5596 6274 6685 6832 7106
Size: 1743 1317 282 521 678 411 147 274
Cuts at: 7106 1733
Size: 2513
Fragments arranged by size:
2513 1743 1317 678 521 411 282 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1116 1146 2041 2071 3652 3682 1116
Size: 30 895 30 1581 30 5320
Fragments arranged by size:
5320 1581 895 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 449 526 885 1207 1526 2065 2070 2320 2405
Size: 77 359 322 319 539 5 250 85
Cuts at: 2405 2429 3181 3537 4627 4824 4885 449
Size: 24 752 356 1090 197 61 3450
Fragments arranged by size:
3450 1090 752 539 359 356 322 319
250 197 85 77 61 24 5
BseYI C'CCAG_C
Cuts at: 728 2749 2986 3291 3499 3588 4064 5311 6069
Size: 2021 237 305 208 89 476 1247 758
Cuts at: 6069 6734 7142 728
Size: 665 408 1472
Fragments arranged by size:
2021 1472 1247 758 665 476 408 305
237 208 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2350 3199 3658 4184 1870
Size: 480 849 459 526 5572
Fragments arranged by size:
5572 849 526 480 459
BsiEI CG_ry'CG
Cuts at: 104 5428 5474 6704 104
Size: 5324 46 1230 1286
Fragments arranged by size:
5324 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 208 749 1230 1531 2575 3761 3995 4421 4900
Size: 541 481 301 1044 1186 234 426 479
Cuts at: 4900 5678 5868 6728 208
Size: 778 190 860 1366
Fragments arranged by size:
1366 1186 1044 860 778 541 481 479
426 301 234 190
BsmI GAATG_Cn'
Cuts at: 1203 2990 3493 3858 1203
Size: 1787 503 365 5231
Fragments arranged by size:
5231 1787 503 365
BsmBI CGTCTCn'nnnn_
Cuts at: 406 967 2265 2411 406
Size: 561 1298 146 5881
Fragments arranged by size:
5881 1298 561 146
Bsp1286I G_dGCh'C
Cuts at: 208 749 1230 1337 1531 1935 2575 2987 3369
Size: 541 481 107 194 404 640 412 382
Cuts at: 3369 3761 3847 3995 4063 4252 4421 4572 4622
Size: 392 86 148 68 189 169 151 50
Cuts at: 4622 4747 4900 5511 5604 5678 5868 5930 6728
Size: 125 153 611 93 74 190 62 798
Cuts at: 6728 208
Size: 1366
Fragments arranged by size:
1366 798 640 611 541 481 412 404
392 382 194 190 189 169 153 151
148 125 107 93 86 74 68 62
50
BspEI T'CCGG_A
Cuts at: 3476 4793 3476
Size: 1317 6569
BspHI T'CATG_A
Cuts at: 6318 6318
Size: 7886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1021 3221 3267 3720 4936 5150 5452 5833 1021
Size: 2200 46 453 1216 214 302 381 3074
Fragments arranged by size:
3074 2200 1216 453 381 302 214 46
BsrBI CCG'CTC
Cuts at: 50 855 6178 6232 7716 7881 50
Size: 805 5323 54 1484 165 55
Fragments arranged by size:
5323 1484 805 165 55 54
BsrDI GCAATG_nn'
Cuts at: 607 1445 1607 4224 4821 5798 607
Size: 838 162 2617 597 977 2695
Fragments arranged by size:
2695 2617 977 838 597 162
BsrFI r'CCGG_y
Cuts at: 762 5884 6065 762
Size: 5122 181 2583
Fragments arranged by size:
5122 2583 181
BsrGI T'GTAC_A
Cuts at: 4286 4286
Size: 7886
BssSI C'ACGA_G
Cuts at: 6157 6865 6157
Size: 708 7178
BstAPI GCAn_nnn'nTGC
Cuts at: 1623 1628 3312 5263 1623
Size: 5 1684 1951 4246
Fragments arranged by size:
4246 1951 1684 5
BstBI TT'CG_AA
Cuts at: 6247 6247
Size: 7886
BstEII G'GTnAC_C
Cuts at: 1288 1288
Size: 7886
BstXI CCAn_nnnn'nTGG
Cuts at: 1923 2862 4348 1923
Size: 939 1486 5461
Fragments arranged by size:
5461 1486 939
BstYI r'GATC_y
Cuts at: 1 434 1140 1235 1668 1775 1799 1963 3255
Size: 433 706 95 433 107 24 164 1292
Cuts at: 3255 4254 4760 4911 5378 5401 5736 5982 6288
Size: 999 506 151 467 23 335 246 306
Cuts at: 6288 6300 6386 7438 1
Size: 12 86 1052 449
Fragments arranged by size:
1292 1052 999 706 506 467 449 433
433 335 306 246 164 151 107 95
86 24 23 12
BstZ17I GTA'TAC
Cuts at: 2336 2336
Size: 7886
Bsu36I CC'TnA_GG
Cuts at: 1190 3089 1190
Size: 1899 5987
BtgI C'CryG_G
Cuts at: 326 3677 4865 326
Size: 3351 1188 3347
Fragments arranged by size:
3351 3347 1188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5836 6017 6180 5836
Size: 181 163 7542
Fragments arranged by size:
7542 181 163
BtsI GCAGTG_nn'
Cuts at: 112 614 690 1378 2391 2570 4076 4165 5129
Size: 502 76 688 1013 179 1506 89 964
Cuts at: 5129 112
Size: 2869
Fragments arranged by size:
2869 1506 1013 964 688 502 179 89
76
DraI TTT'AAA
Cuts at: 4885 4885
Size: 7886
DraIII CAC_nnn'GTG
Cuts at: 3956 3956
Size: 7886
DrdI GACnn_nn'nnGTC
Cuts at: 5592 6936 5592
Size: 1344 6542
EaeI y'GGCC_r
Cuts at: 1023 1559 1754 2175 2582 3818 5471 5645 6036
Size: 536 195 421 407 1236 1653 174 391
Cuts at: 6036 6063 7408 1023
Size: 27 1345 1501
Fragments arranged by size:
1653 1501 1345 1236 536 421 407 391
195 174 27
EagI C'GGCC_G
Cuts at: 5471 5471
Size: 7886
EarI CTCTTCn'nnn_
Cuts at: 196 1463 2233 4240 4525 5909 6119 196
Size: 1267 770 2007 285 1384 210 1963
Fragments arranged by size:
2007 1963 1384 1267 770 285 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6094 6836 6982 7696 6094
Size: 742 146 714 6284
Fragments arranged by size:
6284 742 714 146
EcoICRI GAG'CTC
Cuts at: 3759 3993 4898 3759
Size: 234 905 6747
Fragments arranged by size:
6747 905 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1246 2426 2752 2799 2954 2974 3253 3679
Size: 873 1180 326 47 155 20 279 426
Cuts at: 3679 3739 3845 3994 4006 4246 4261 5711 6143
Size: 60 106 149 12 240 15 1450 432
Cuts at: 6143 6496 7158 373
Size: 353 662 1101
Fragments arranged by size:
1450 1180 1101 873 662 432 426 353
326 279 240 155 149 106 60 47
20 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3832 3832
Size: 7886
EcoO109I rG'GnC_Cy
Cuts at: 382 1171 1938 2983 3365 3366 3872 4569 4618
Size: 789 767 1045 382 1 506 697 49
Cuts at: 4618 5025 382
Size: 407 3243
Fragments arranged by size:
3243 1045 789 767 697 506 407 382
49 1
EcoRI G'AATT_C
Cuts at: 3057 3352 4890 3057
Size: 295 1538 6053
Fragments arranged by size:
6053 1538 295
EcoRV GAT'ATC
Cuts at: 504 504
Size: 7886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2454 4218 4251 5905 2454
Size: 1764 33 1654 4435
Fragments arranged by size:
4435 1764 1654 33
FspI TGC'GCA
Cuts at: 5667 5667
Size: 7886
HaeII r_GCGC'y
Cuts at: 5320 5568 6798 7569 5320
Size: 248 1230 771 5637
Fragments arranged by size:
5637 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 348 380 1186 1218 1462 1494 1876 1908 2113
Size: 32 806 32 244 32 382 32 205
Cuts at: 2113 2145 2267 2299 2384 2416 2764 2796 3328
Size: 32 122 32 85 32 348 32 532
Cuts at: 3328 3360 3559 3591 4239 4246 4271 4278 4788
Size: 32 199 32 648 7 25 7 510
Cuts at: 4788 4820 5728 5758 5760 5790 348
Size: 32 908 30 2 30 2444
Fragments arranged by size:
2444 908 806 648 532 510 382 348
244 205 199 122 85 32 32 32
32 32 32 32 32 32 32 32
30 30 25 7 7 2
HincII GTy'rAC
Cuts at: 4925 4925
Size: 7886
KasI G'GCGC_C
Cuts at: 5316 5564 5316
Size: 248 7638
KpnI G_GTAC'C
Cuts at: 4906 4906
Size: 7886
MluI A'CGCG_T
Cuts at: 7319 7319
Size: 7886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 350 2298 2681 6645 6829 350
Size: 1948 383 3964 184 1407
Fragments arranged by size:
3964 1948 1407 383 184
MscI TGG'CCA
Cuts at: 1025 1756 2177 2584 5647 1025
Size: 731 421 407 3063 3264
Fragments arranged by size:
3264 3063 731 421 407
MslI CAynn'nnrTG
Cuts at: 175 328 1750 1756 1921 2095 3625 4520 4769
Size: 153 1422 6 165 174 1530 895 249
Cuts at: 4769 5875 6002 175
Size: 1106 127 2059
Fragments arranged by size:
2059 1530 1422 1106 895 249 174 165
153 127 6
MspA1I CmG'CkG
Cuts at: 783 1266 1695 2952 3657 3880 4068 4471 4730
Size: 483 429 1257 705 223 188 403 259
Cuts at: 4730 4997 5311 5671 6455 6700 783
Size: 267 314 360 784 245 1969
Fragments arranged by size:
1969 1257 784 705 483 429 403 360
314 267 259 245 223 188
NaeI GCC'GGC
Cuts at: 6067 6067
Size: 7886
NarI GG'CG_CC
Cuts at: 5317 5565 5317
Size: 248 7638
NcoI C'CATG_G
Cuts at: 326 3677 326
Size: 3351 4535
NdeI CA'TA_TG
Cuts at: 174 2901 4766 174
Size: 2727 1865 3294
Fragments arranged by size:
3294 2727 1865
NgoMIV G'CCGG_C
Cuts at: 6065 6065
Size: 7886
NsiI A_TGCA'T
Cuts at: 1203 3162 3858 1203
Size: 1959 696 5231
Fragments arranged by size:
5231 1959 696
NspI r_CATG'y
Cuts at: 1094 1356 1626 2342 2528 2547 3415 4939 5439
Size: 262 270 716 186 19 868 1524 500
Cuts at: 5439 7042 1094
Size: 1603 1938
Fragments arranged by size:
1938 1603 1524 868 716 500 270 262
186 19
PasI CC'CwG_GG
Cuts at: 1935 1935
Size: 7886
PciI A'CATG_T
Cuts at: 2338 2543 7038 2338
Size: 205 4495 3186
Fragments arranged by size:
4495 3186 205
PflMI CCAn_nnn'nTGG
Cuts at: 527 1285 527
Size: 758 7128
PfoI T'CCnGG_A
Cuts at: 1313 1814 3923 4238 1313
Size: 501 2109 315 4961
Fragments arranged by size:
4961 2109 501 315
PmeI GTTT'AAAC
Cuts at: 4885 4885
Size: 7886
PmlI CAC'GTG
Cuts at: 1485 2328 1485
Size: 843 7043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2073 6332 6364 2041
Size: 32 4259 32 3563
Fragments arranged by size:
4259 3563 32 32
PpuMI rG'GwC_Cy
Cuts at: 1171 1171
Size: 7886
PshAI GACnn'nnGTC
Cuts at: 281 281
Size: 7886
PspOMI G'GGCC_C
Cuts at: 2983 3365 4568 4618 2983
Size: 382 1203 50 6251
Fragments arranged by size:
6251 1203 382 50
PspXI vC'TCGA_Gb
Cuts at: 2411 2411
Size: 7886
PstI C_TGCA'G
Cuts at: 1271 1505 1633 1694 1704 3711 3731 4262 4933
Size: 234 128 61 10 2007 20 531 671
Cuts at: 4933 5618 1271
Size: 685 3539
Fragments arranged by size:
3539 2007 685 671 531 234 128 61
20 10
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7886
PvuII CAG'CTG
Cuts at: 783 1266 2952 3657 4068 4730 5311 5671 783
Size: 483 1686 705 411 662 581 360 2998
Fragments arranged by size:
2998 1686 705 662 581 483 411 360
RsrII CG'GwC_CG
Cuts at: 6081 6081
Size: 7886
SacI G_AGCT'C
Cuts at: 3761 3995 4900 3761
Size: 234 905 6747
Fragments arranged by size:
6747 905 234
SalI G'TCGA_C
Cuts at: 4923 4923
Size: 7886
SapI GCTCTTCn'nnn_
Cuts at: 196 1463 4240 5909 6119 196
Size: 1267 2777 1669 210 1963
Fragments arranged by size:
2777 1963 1669 1267 210
SbfI CC_TGCA'GG
Cuts at: 1505 4933 1505
Size: 3428 4458
ScaI AGT'ACT
Cuts at: 7403 7403
Size: 7886
SfcI C'TryA_G
Cuts at: 33 212 1267 1501 1629 1690 1700 3707 3727
Size: 179 1055 234 128 61 10 2007 20
Cuts at: 3727 4258 4929 5614 6582 6773 33
Size: 531 671 685 968 191 1146
Fragments arranged by size:
2007 1146 1055 968 685 671 531 234
191 179 128 61 20 10
SfoI GGC'GCC
Cuts at: 5318 5566 5318
Size: 248 7638
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7886
SmaI CCC'GGG
Cuts at: 805 4669 4908 805
Size: 3864 239 3783
Fragments arranged by size:
3864 3783 239
SmlI C'TyrA_G
Cuts at: 1459 2411 4111 4456 5041 5956 6670 6932 1459
Size: 952 1700 345 585 915 714 262 2413
Fragments arranged by size:
2413 1700 952 915 714 585 345 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7886
SphI G_CATG'C
Cuts at: 1356 1626 3415 4939 5439 1356
Size: 270 1789 1524 500 3803
Fragments arranged by size:
3803 1789 1524 500 270
StuI AGG'CCT
Cuts at: 1507 1595 2884 4781 1507
Size: 88 1289 1897 4612
Fragments arranged by size:
4612 1897 1289 88
StyI C'CwwG_G
Cuts at: 326 557 1055 1100 1167 1250 1949 2172 3677
Size: 231 498 45 67 83 699 223 1505
Cuts at: 3677 4085 4341 4721 4748 5020 5374 326
Size: 408 256 380 27 272 354 2838
Fragments arranged by size:
2838 1505 699 498 408 380 354 272
256 231 223 83 67 45 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 680 4078 4354 5469 5602 6061 6270 680
Size: 3398 276 1115 133 459 209 2296
Fragments arranged by size:
3398 2296 1115 459 276 209 133
TatI w'GTAC_w
Cuts at: 189 1878 2435 2540 3981 4286 4577 7401 189
Size: 1689 557 105 1441 305 291 2824 674
Fragments arranged by size:
2824 1689 1441 674 557 305 291 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 997 1029 1220 1252 2190 2222 7403 7435 997
Size: 32 191 32 938 32 5181 32 1448
Fragments arranged by size:
5181 1448 938 191 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5683 5683
Size: 7886
XbaI T'CTAG_A
Cuts at: 4917 4917
Size: 7886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1033 5381 1033
Size: 4348 3538
XhoI C'TCGA_G
Cuts at: 2411 2411
Size: 7886
XmaI C'CCGG_G
Cuts at: 803 4667 4906 803
Size: 3864 239 3783
Fragments arranged by size:
3864 3783 239
XmnI GAAnn'nnTTC
Cuts at: 1431 2356 2740 2765 4234 1431
Size: 925 384 25 1469 5083
Fragments arranged by size:
5083 1469 925 384 25
ZraI GAC'GTC
Cuts at: 1821 2220 1821
Size: 399 7487
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AscI BaeI BcgI BmgBI BmtI BsiWI
BssHII ClaI CspCI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI PacI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI