Restriction map for
pF1KB0006
(Circular) (Six-base) MAPSORT of: pF1KB0006.seq Check: 4786 from: 1 to: 9908
pF1KB0006 9908 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1751 1751
Size: 9908
AccI GT'mk_AC
Cuts at: 1052 1781 2135 2518 3557 4130 4609 6946 1052
Size: 729 354 383 1039 573 479 2337 4014
Fragments arranged by size:
4014 2337 1039 729 573 479 383 354
Acc65I G'GTAC_C
Cuts at: 6924 6924
Size: 9908
AclI AA'CG_TT
Cuts at: 9748 9748
Size: 9908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 407 1096 2140 3488 4046 4281 5041 5785
Size: 6 689 1044 1348 558 235 760 744
Cuts at: 5785 7733 8165 8912 9180 401
Size: 1948 432 747 268 1129
Fragments arranged by size:
1948 1348 1129 1044 760 747 744 689
558 432 268 235 6
AflII C'TTAA_G
Cuts at: 5221 5221
Size: 9908
AflIII A'CryG_T
Cuts at: 596 1597 6574 6625 7260 8364 9341 596
Size: 1001 4977 51 635 1104 977 1163
Fragments arranged by size:
4977 1163 1104 1001 977 635 51
AgeI A'CCGG_T
Cuts at: 8296 8296
Size: 9908
AhdI GACnn_n'nnGTC
Cuts at: 1967 2346 1967
Size: 379 9529
AleI CACnn'nnGTG
Cuts at: 931 2221 2428 3397 3415 3673 4298 931
Size: 1290 207 969 18 258 625 6541
Fragments arranged by size:
6541 1290 969 625 258 207 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 543 4259 543
Size: 3716 6192
AlwNI CAG_nnn'CTG
Cuts at: 156 1074 1804 2403 2539 2967 4450 4977 5467
Size: 918 730 599 136 428 1483 527 490
Cuts at: 5467 7213 8780 156
Size: 1746 1567 1284
Fragments arranged by size:
1746 1567 1483 1284 918 730 599 527
490 428 136
ApaI G_GGCC'C
Cuts at: 128 3174 6117 128
Size: 3046 2943 3919
Fragments arranged by size:
3919 3046 2943
ApaLI G'TGCA_C
Cuts at: 1593 4234 8678 1593
Size: 2641 4444 2823
Fragments arranged by size:
4444 2823 2641
ApoI r'AATT_y
Cuts at: 1347 2052 3762 4338 6598 6912 9261 9517 1347
Size: 705 1710 576 2260 314 2349 256 1738
Fragments arranged by size:
2349 2260 1738 1710 705 576 314 256
AseI AT'TA_AT
Cuts at: 20 4715 20
Size: 4695 5213
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9908
AvaI C'yCGr_G
Cuts at: 127 1337 3167 4250 4288 4310 6896 6928 127
Size: 1210 1830 1083 38 22 2586 32 3107
Fragments arranged by size:
3107 2586 1830 1210 1083 38 32 22
AvrII C'CTAG_G
Cuts at: 315 315
Size: 9908
BamHI G'GATC_C
Cuts at: 4274 6933 4274
Size: 2659 7249
BanI G'GyrC_C
Cuts at: 168 1357 2806 3486 4323 6238 6494 6685 6924
Size: 1189 1449 680 837 1915 256 191 239
Cuts at: 6924 7338 7586 7621 168
Size: 414 248 35 2455
Fragments arranged by size:
2455 1915 1449 1189 837 680 414 256
248 239 191 35
BanII G_rGCy'C
Cuts at: 128 3174 3334 3376 3799 3972 5298 5515 5789
Size: 3046 160 42 423 173 1326 217 274
Cuts at: 5789 6117 6922 7952 128
Size: 328 805 1030 2084
Fragments arranged by size:
3046 2084 1326 1030 805 423 328 274
217 173 160 42
BbeI G_GCGC'C
Cuts at: 7342 7590 7342
Size: 248 9660
BbsI GAAGACnn'nnnn_
Cuts at: 1084 4170 5125 6649 1084
Size: 3086 955 1524 4343
Fragments arranged by size:
4343 3086 1524 955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 958 992 1640 1674 6274 6308 90
Size: 34 834 34 648 34 4600 34 3690
Fragments arranged by size:
4600 3690 834 648 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3178 3309 7806 8567 9908 3178
Size: 131 4497 761 1341 3178
Fragments arranged by size:
4497 3178 1341 761 131
BclI T'GATC_A
Cuts at: 669 3419 3789 4502 7428 669
Size: 2750 370 713 2926 3149
Fragments arranged by size:
3149 2926 2750 713 370
BfrBI ATG'CAT
Cuts at: 1631 1631
Size: 9908
BglI GCCn_nnn'nGGC
Cuts at: 6722 6722
Size: 9908
BglII A'GATC_T
Cuts at: 5477 5864 7423 9460 5477
Size: 387 1559 2037 5925
Fragments arranged by size:
5925 2037 1559 387
BlpI GC'TnA_GC
Cuts at: 206 7020 206
Size: 6814 3094
Bme1580I G_kGCm'C
Cuts at: 128 173 1597 1724 2831 3174 4238 4328 6117
Size: 45 1424 127 1107 343 1064 90 1789
Cuts at: 6117 7533 7626 8682 128
Size: 1416 93 1056 1354
Fragments arranged by size:
1789 1424 1416 1354 1107 1064 1056 343
127 93 90 45
BmgBI CAC'GTC
Cuts at: 2868 2868
Size: 9908
BmrI ACTGGGnnnn_n'
Cuts at: 219 422 1814 5052 5963 6758 7120 7201 7536
Size: 203 1392 3238 911 795 362 81 335
Cuts at: 7536 9680 219
Size: 2144 447
Fragments arranged by size:
3238 2144 1392 911 795 447 362 335
203 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 221 3086 3176 4169 4991 8275 221
Size: 2865 90 993 822 3284 1854
Fragments arranged by size:
3284 2865 1854 993 822 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 859 864 2619 3100 3365 3524 3673 3692
Size: 271 5 1755 481 265 159 149 19
Cuts at: 3692 3850 4107 4349 4658 5290 5358 588
Size: 158 257 242 309 632 68 5138
Fragments arranged by size:
5138 1755 632 481 309 271 265 257
242 159 158 149 68 19 5
Bpu10I CC'TnA_GC
Cuts at: 112 5050 5249 6450 112
Size: 4938 199 1201 3570
Fragments arranged by size:
4938 3570 1201 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1914 7084 7963 8455 8753 1914
Size: 5170 879 492 298 3069
Fragments arranged by size:
5170 3069 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 567 2037 2325 2334 3400 4299 5311 5666 9612
Size: 1470 288 9 1066 899 1012 355 3946
Cuts at: 9612 567
Size: 863
Fragments arranged by size:
3946 1470 1066 1012 899 863 355 288
9
BsaAI yAC'GTr
Cuts at: 1109 1598 5617 6654 7261 1109
Size: 489 4019 1037 607 3756
Fragments arranged by size:
4019 3756 1037 607 489
BsaBI GATnn'nnATC
Cuts at: 6 7422 9459 6
Size: 7416 2037 455
Fragments arranged by size:
7416 2037 455
BsaHI Gr'CG_yC
Cuts at: 3430 5531 7339 7587 8289 9785 3430
Size: 2101 1808 248 702 1496 3553
Fragments arranged by size:
3553 2101 1808 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5122 7097 7618 8296 8570 8717 9128 5122
Size: 1975 521 678 274 147 411 5902
Fragments arranged by size:
5902 1975 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1947 1977 2083 2113 3420 3450 3954 3984 4821
Size: 30 106 30 1307 30 504 30 837
Cuts at: 4821 4851 1947
Size: 30 7004
Fragments arranged by size:
7004 1307 837 504 106 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 585 917 1004 1355 2255 2395 3494 3711 3902
Size: 332 87 351 900 140 1099 217 191
Cuts at: 3902 4238 5654 585
Size: 336 1416 4839
Fragments arranged by size:
4839 1416 1099 900 351 336 332 217
191 140 87
BseYI C'CCAG_C
Cuts at: 490 679 1007 3456 3900 4508 7333 8091 8668
Size: 189 328 2449 444 608 2825 758 577
Cuts at: 8668 9164 490
Size: 496 1234
Fragments arranged by size:
2825 2449 1234 758 608 577 496 444
328 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 955 998 1212 1284 1293 1500 2924 3786
Size: 85 43 214 72 9 207 1424 862
Cuts at: 3786 4991 6555 6684 870
Size: 1205 1564 129 4094
Fragments arranged by size:
4094 1564 1424 1205 862 214 207 129
85 72 43 9
BsiEI CG_ry'CG
Cuts at: 104 900 7450 7496 8704 104
Size: 796 6550 46 1208 1308
Fragments arranged by size:
6550 1308 1208 796 46
BsiHKAI G_wGCw'C
Cuts at: 1597 2957 3376 3680 3703 3799 4150 4238 4295
Size: 1360 419 304 23 96 351 88 57
Cuts at: 4295 5515 6922 7700 7890 8682 1597
Size: 1220 1407 778 190 792 2823
Fragments arranged by size:
2823 1407 1360 1220 792 778 419 351
304 190 96 88 57 23
BsmI GAATG_Cn'
Cuts at: 2004 2004
Size: 9908
BsmBI CGTCTCn'nnnn_
Cuts at: 2242 5347 2242
Size: 3105 6803
Bsp1286I G_dGCh'C
Cuts at: 128 173 1597 1724 2831 2957 3174 3334 3376
Size: 45 1424 127 1107 126 217 160 42
Cuts at: 3376 3680 3703 3799 3972 4150 4238 4295 4328
Size: 304 23 96 173 178 88 57 33
Cuts at: 4328 5298 5515 5789 6117 6922 7533 7626 7700
Size: 970 217 274 328 805 611 93 74
Cuts at: 7700 7890 7952 8682 128
Size: 190 62 730 1354
Fragments arranged by size:
1424 1354 1107 970 805 730 611 328
304 274 217 217 190 178 173 160
127 126 96 93 88 74 62 57
45 42 33 23
BspEI T'CCGG_A
Cuts at: 9128 9128
Size: 9908
BspHI T'CATG_A
Cuts at: 1710 6615 9084 1710
Size: 4905 2469 2534
Fragments arranged by size:
4905 2534 2469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 487 649 878 1751 2453 2852 2951 3932 4867
Size: 162 229 873 702 399 99 981 935
Cuts at: 4867 5570 6958 7172 7474 7855 487
Size: 703 1388 214 302 381 2540
Fragments arranged by size:
2540 1388 981 935 873 703 702 399
381 302 229 214 162 99
BsrBI CCG'CTC
Cuts at: 50 306 950 8200 8254 9738 9903 50
Size: 256 644 7250 54 1484 165 55
Fragments arranged by size:
7250 1484 644 256 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 1631 1899 5756 7820 327
Size: 1304 268 3857 2064 2415
Fragments arranged by size:
3857 2415 2064 1304 268
BsrFI r'CCGG_y
Cuts at: 2911 5629 7906 8087 8296 2911
Size: 2718 2277 181 209 4523
Fragments arranged by size:
4523 2718 2277 209 181
BsrGI T'GTAC_A
Cuts at: 1735 5486 6873 1735
Size: 3751 1387 4770
Fragments arranged by size:
4770 3751 1387
BssSI C'ACGA_G
Cuts at: 1504 3304 3491 5297 6357 8179 8537 1504
Size: 1800 187 1806 1060 1822 358 2875
Fragments arranged by size:
2875 1822 1806 1800 1060 358 187
BstAPI GCAn_nnn'nTGC
Cuts at: 779 2967 7285 779
Size: 2188 4318 3402
Fragments arranged by size:
4318 3402 2188
BstBI TT'CG_AA
Cuts at: 1276 8269 1276
Size: 6993 2915
BstEII G'GTnAC_C
Cuts at: 2874 3018 5103 5829 2874
Size: 144 2085 726 6953
Fragments arranged by size:
6953 2085 726 144
BstXI CCAn_nnnn'nTGG
Cuts at: 1480 4002 5676 6505 1480
Size: 2522 1674 829 4883
Fragments arranged by size:
4883 2522 1674 829
BstYI r'GATC_y
Cuts at: 1 952 2406 4274 5477 5864 6933 7400 7423
Size: 951 1454 1868 1203 387 1069 467 23
Cuts at: 7423 7758 8004 9016 9102 9114 9460 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1868 1454 1203 1069 1012 951 467 449
387 346 335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2136 2136
Size: 9908
BtgI C'CryG_G
Cuts at: 176 1493 1881 3051 3197 4677 5165 6311 176
Size: 1317 388 1170 146 1480 488 1146 3773
Fragments arranged by size:
3773 1480 1317 1170 1146 488 388 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 501 7858 8039 8202 501
Size: 7357 181 163 2207
Fragments arranged by size:
7357 2207 181 163
BtsI GCAGTG_nn'
Cuts at: 1075 2912 3050 3358 4079 5537 7151 1075
Size: 1837 138 308 721 1458 1614 3832
Fragments arranged by size:
3832 1837 1614 1458 721 308 138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5526 5561 5526
Size: 35 9873
DraI TTT'AAA
Cuts at: 6432 6907 6432
Size: 475 9433
DrdI GACnn_nn'nnGTC
Cuts at: 2872 3627 7614 8472 2872
Size: 755 3987 858 4308
Fragments arranged by size:
4308 3987 858 755
EaeI y'GGCC_r
Cuts at: 143 897 1023 1878 2169 2273 2454 3308 3750
Size: 754 126 855 291 104 181 854 442
Cuts at: 3750 4431 4674 5189 5394 5573 6032 6555 6783
Size: 681 243 515 205 179 459 523 228
Cuts at: 6783 7493 7667 8058 8085 9430 143
Size: 710 174 391 27 1345 621
Fragments arranged by size:
1345 855 854 754 710 681 621 523
515 459 442 391 291 243 228 205
181 179 174 126 104 27
EagI C'GGCC_G
Cuts at: 897 7493 897
Size: 6596 3312
EarI CTCTTCn'nnn_
Cuts at: 184 383 1419 1709 3207 5773 6293 7931 8141
Size: 199 1036 290 1498 2566 520 1638 210
Cuts at: 8141 184
Size: 1951
Fragments arranged by size:
2566 1951 1638 1498 1036 520 290 210
199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4267 6027 8116 8426 8572 9718 4267
Size: 1760 2089 310 146 1146 4457
Fragments arranged by size:
4457 2089 1760 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3374 3797 5513 6920 3374
Size: 423 1716 1407 6362
Fragments arranged by size:
6362 1716 1407 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 407 588 859 864 1096 2140 2619 3100
Size: 6 181 271 5 232 1044 479 481
Cuts at: 3100 3365 3488 3524 3673 3692 3850 4046 4107
Size: 265 123 36 149 19 158 196 61
Cuts at: 4107 4281 4349 4658 5041 5290 5358 5785 7733
Size: 174 68 309 383 249 68 427 1948
Cuts at: 7733 8165 8912 9180 401
Size: 432 747 268 1129
Fragments arranged by size:
1948 1129 1044 747 481 479 432 427
383 309 271 268 265 249 232 196
181 174 158 149 123 68 68 61
36 19 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2835 3693 4157 2835
Size: 858 464 8586
Fragments arranged by size:
8586 858 464
EcoO109I rG'GnC_Cy
Cuts at: 124 2841 3241 4464 6114 7047 124
Size: 2717 400 1223 1650 933 2985
Fragments arranged by size:
2985 2717 1650 1223 933 400
EcoRI G'AATT_C
Cuts at: 4338 6598 6912 4338
Size: 2260 314 7334
Fragments arranged by size:
7334 2260 314
EcoRV GAT'ATC
Cuts at: 2486 5092 2486
Size: 2606 7302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7927 7927
Size: 9908
FspI TGC'GCA
Cuts at: 7689 7689
Size: 9908
HaeII r_GCGC'y
Cuts at: 2539 5057 6732 7342 7590 8612 9591 2539
Size: 2518 1675 610 248 1022 979 2856
Fragments arranged by size:
2856 2518 1675 1022 979 610 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1800 1832 1953 1985 2332 2364 2566 2598 3150
Size: 32 121 32 347 32 202 32 552
Cuts at: 3150 3182 3420 3452 3781 3813 4233 4244 4265
Size: 32 238 32 329 32 420 11 21
Cuts at: 4265 4266 4276 4298 4726 4758 5146 5178 5539
Size: 1 10 22 428 32 388 32 361
Cuts at: 5539 5571 5663 5695 5849 5881 6801 6833 7750
Size: 32 92 32 154 32 920 32 917
Cuts at: 7750 7780 7782 7812 1800
Size: 30 2 30 3896
Fragments arranged by size:
3896 920 917 552 428 420 388 361
347 329 238 202 154 121 92 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 22 21
11 10 2 1
HincII GTy'rAC
Cuts at: 1782 2940 6327 6947 1782
Size: 1158 3387 620 4743
Fragments arranged by size:
4743 3387 1158 620
KasI G'GCGC_C
Cuts at: 7338 7586 7338
Size: 248 9660
KpnI G_GTAC'C
Cuts at: 6928 6928
Size: 9908
MfeI C'AATT_G
Cuts at: 2585 3602 6848 2585
Size: 1017 3246 5645
Fragments arranged by size:
5645 3246 1017
MluI A'CGCG_T
Cuts at: 9341 9341
Size: 9908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 678 2047 2363 5582 6375 8579 8763 678
Size: 1369 316 3219 793 2204 184 1823
Fragments arranged by size:
3219 2204 1823 1369 793 316 184
MscI TGG'CCA
Cuts at: 1025 2275 3310 4433 5191 5396 5575 6557 6785
Size: 1250 1035 1123 758 205 179 982 228
Cuts at: 6785 7669 1025
Size: 884 3264
Fragments arranged by size:
3264 1250 1123 1035 982 884 758 228
205 179
MslI CAynn'nnrTG
Cuts at: 931 2221 2428 2881 3397 3415 3523 3673 4298
Size: 1290 207 453 516 18 108 150 625
Cuts at: 4298 4657 6256 6489 6503 7897 8024 931
Size: 359 1599 233 14 1394 127 2815
Fragments arranged by size:
2815 1599 1394 1290 625 516 453 359
233 207 150 127 108 18 14
MspA1I CmG'CkG
Cuts at: 166 767 982 1130 1144 3904 4508 4625 6720
Size: 601 215 148 14 2760 604 117 2095
Cuts at: 6720 7019 7333 7693 8706 8951 166
Size: 299 314 360 1013 245 1123
Fragments arranged by size:
2760 2095 1123 1013 604 601 360 314
299 245 215 148 117 14
NaeI GCC'GGC
Cuts at: 8089 8089
Size: 9908
NarI GG'CG_CC
Cuts at: 7339 7587 7339
Size: 248 9660
NcoI C'CATG_G
Cuts at: 1493 3051 1493
Size: 1558 8350
NgoMIV G'CCGG_C
Cuts at: 8087 8087
Size: 9908
NsiI A_TGCA'T
Cuts at: 1633 1633
Size: 9908
NspI r_CATG'y
Cuts at: 600 1631 1728 1742 6578 6707 6961 7461 8368
Size: 1031 97 14 4836 129 254 500 907
Cuts at: 8368 600
Size: 2140
Fragments arranged by size:
4836 2140 1031 907 500 254 129 97
14
PasI CC'CwG_GG
Cuts at: 2837 3013 5914 2837
Size: 176 2901 6831
Fragments arranged by size:
6831 2901 176
PciI A'CATG_T
Cuts at: 596 6574 8364 596
Size: 5978 1790 2140
Fragments arranged by size:
5978 2140 1790
PflMI CCAn_nnn'nTGG
Cuts at: 156 467 1994 2885 3445 5336 5392 6110 156
Size: 311 1527 891 560 1891 56 718 3954
Fragments arranged by size:
3954 1891 1527 891 718 560 311 56
PfoI T'CCnGG_A
Cuts at: 3540 4580 6122 6195 3540
Size: 1040 1542 73 7253
Fragments arranged by size:
7253 1542 1040 73
PmeI GTTT'AAAC
Cuts at: 6907 6907
Size: 9908
PmlI CAC'GTG
Cuts at: 1109 1598 5617 1109
Size: 489 4019 5400
Fragments arranged by size:
5400 4019 489
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9047 9079 9047
Size: 32 9876
PpuMI rG'GwC_Cy
Cuts at: 2841 3241 4464 2841
Size: 400 1223 8285
Fragments arranged by size:
8285 1223 400
PshAI GACnn'nnGTC
Cuts at: 1577 1577
Size: 9908
PspOMI G'GGCC_C
Cuts at: 124 3170 6113 124
Size: 3046 2943 3919
Fragments arranged by size:
3919 3046 2943
PspXI vC'TCGA_Gb
Cuts at: 1337 4250 1337
Size: 2913 6995
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5727 5759 5727
Size: 32 9876
PstI C_TGCA'G
Cuts at: 2849 3278 3365 4878 4992 6955 7640 2849
Size: 429 87 1513 114 1963 685 5117
Fragments arranged by size:
5117 1963 1513 685 429 114 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9908
PvuII CAG'CTG
Cuts at: 982 1130 1144 3904 4508 7333 7693 982
Size: 148 14 2760 604 2825 360 3197
Fragments arranged by size:
3197 2825 2760 604 360 148 14
RsrII CG'GwC_CG
Cuts at: 8103 8103
Size: 9908
SacI G_AGCT'C
Cuts at: 3376 3799 5515 6922 3376
Size: 423 1716 1407 6362
Fragments arranged by size:
6362 1716 1407 423
SalI G'TCGA_C
Cuts at: 1780 6945 1780
Size: 5165 4743
SanDI GG'GwC_CC
Cuts at: 3241 3241
Size: 9908
SapI GCTCTTCn'nnn_
Cuts at: 184 5773 7931 8141 184
Size: 5589 2158 210 1951
Fragments arranged by size:
5589 2158 1951 210
SbfI CC_TGCA'GG
Cuts at: 6955 6955
Size: 9908
ScaI AGT'ACT
Cuts at: 9425 9425
Size: 9908
SexAI A'CCwGG_T
Cuts at: 2721 2721
Size: 9908
SfcI C'TryA_G
Cuts at: 33 825 2057 2845 3274 3361 4269 4610 4874
Size: 792 1232 788 429 87 908 341 264
Cuts at: 4874 4988 6951 7636 8629 8820 33
Size: 114 1963 685 993 191 1121
Fragments arranged by size:
1963 1232 1121 993 908 792 788 685
429 341 264 191 114 87
SfoI GGC'GCC
Cuts at: 7340 7588 7340
Size: 248 9660
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9908
SmaI CCC'GGG
Cuts at: 129 6930 129
Size: 6801 3107
SmlI C'TyrA_G
Cuts at: 1337 1929 4250 4310 5221 7063 7978 8470 8732
Size: 592 2321 60 911 1842 915 492 262
Cuts at: 8732 1337
Size: 2513
Fragments arranged by size:
2513 2321 1842 915 911 592 492 262
60
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9908
SphI G_CATG'C
Cuts at: 6961 7461 6961
Size: 500 9408
SrfI GCCC'GGGC
Cuts at: 129 129
Size: 9908
StuI AGG'CCT
Cuts at: 2117 6227 2117
Size: 4110 5798
StyI C'CwwG_G
Cuts at: 315 723 1493 3051 5111 7042 7396 315
Size: 408 770 1558 2060 1931 354 2827
Fragments arranged by size:
2827 2060 1931 1558 770 408 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1735 2308 3448 4774 5055 5085 5598 5923
Size: 1314 573 1140 1326 281 30 513 325
Cuts at: 5923 7491 7624 8083 8292 421
Size: 1568 133 459 209 2037
Fragments arranged by size:
2037 1568 1326 1314 1140 573 513 459
325 281 209 133 30
TatI w'GTAC_w
Cuts at: 334 407 745 1735 2027 2773 3230 3290 3833
Size: 73 338 990 292 746 457 60 543
Cuts at: 3833 5486 6137 6873 9423 334
Size: 1653 651 736 2550 819
Fragments arranged by size:
2550 1653 990 819 746 736 651 543
457 338 292 73 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1599 1631 1947 1979 2083 2115 2345 2377 3954
Size: 32 316 32 104 32 230 32 1577
Cuts at: 3954 3986 5081 5113 5171 5203 6343 6375 9425
Size: 32 1095 32 58 32 1140 32 3050
Cuts at: 9425 9457 1599
Size: 32 2050
Fragments arranged by size:
3050 2050 1577 1140 1095 316 230 104
58 32 32 32 32 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 7705 7705
Size: 9908
XbaI T'CTAG_A
Cuts at: 787 6487 6939 787
Size: 5700 452 3756
Fragments arranged by size:
5700 3756 452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1159 2313 5592 6391 7403 1159
Size: 1154 3279 799 1012 3664
Fragments arranged by size:
3664 3279 1154 1012 799
XhoI C'TCGA_G
Cuts at: 1337 4250 4310 1337
Size: 2913 60 6935
Fragments arranged by size:
6935 2913 60
XmaI C'CCGG_G
Cuts at: 127 6928 127
Size: 6801 3107
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AloI AscI BaeI BbvCI BmtI BsiWI
BssHII Bsu36I ClaI DraIII FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PsiI SacII SfiI
SgrAI SnaBI SspI SwaI XmnI ZraI