Restriction map for
pF1KA2034
(Circular) (Six-base) MAPSORT of: pF1KA2034.seq Check: 5704 from: 1 to: 9221
pF1KA2034 9221 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 237 1901 2808 237
Size: 1664 907 6650
Fragments arranged by size:
6650 1664 907
AatII G_ACGT'C
Cuts at: 4850 4850
Size: 9221
AccI GT'mk_AC
Cuts at: 1940 6259 1940
Size: 4319 4902
Acc65I G'GTAC_C
Cuts at: 6237 6237
Size: 9221
AclI AA'CG_TT
Cuts at: 9061 9061
Size: 9221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1896 2653 3469 3838 5188 5947 7046 7478
Size: 1064 757 816 369 1350 759 1099 432
Cuts at: 7478 7831 8493 832
Size: 353 662 1560
Fragments arranged by size:
1560 1350 1099 1064 816 759 757 662
432 369 353
AflIII A'CryG_T
Cuts at: 512 626 6573 8373 8654 512
Size: 114 5947 1800 281 1079
Fragments arranged by size:
5947 1800 1079 281 114
AgeI A'CCGG_T
Cuts at: 1112 1112
Size: 9221
AhdI GACnn_n'nnGTC
Cuts at: 3663 4019 3663
Size: 356 8865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2139 4876 2139
Size: 2737 6484
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1792 1824 5645 5677 1792
Size: 32 3821 32 5336
Fragments arranged by size:
5336 3821 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1053 2113 2541 2862 3039 3186 3438 3609 3870
Size: 1060 428 321 177 147 252 171 261
Cuts at: 3870 3894 4248 4503 4566 4650 5649 6526 7964
Size: 24 354 255 63 84 999 877 1438
Cuts at: 7964 1053
Size: 2310
Fragments arranged by size:
2310 1438 1060 999 877 428 354 321
261 255 252 177 171 147 84 63
24
ApaI G_GGCC'C
Cuts at: 163 209 2856 3531 3597 3603 4737 4806 5706
Size: 46 2647 675 66 6 1134 69 900
Cuts at: 5706 163
Size: 3678
Fragments arranged by size:
3678 2647 1134 900 675 69 66 46
6
ApaLI G'TGCA_C
Cuts at: 8059 8059
Size: 9221
ApoI r'AATT_y
Cuts at: 922 3226 3261 6225 8574 8830 922
Size: 2304 35 2964 2349 256 1313
Fragments arranged by size:
2964 2349 2304 1313 256 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 9221
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9221
AvaI C'yCGr_G
Cuts at: 133 169 231 1093 1367 2672 3596 4289 4475
Size: 36 62 862 274 1305 924 693 186
Cuts at: 4475 4730 5282 5963 6241 133
Size: 255 552 681 278 3113
Fragments arranged by size:
3113 1305 924 862 693 681 552 278
274 255 186 62 36
BamHI G'GATC_C
Cuts at: 6246 6246
Size: 9221
BanI G'GyrC_C
Cuts at: 129 144 235 279 615 964 1540 1889 4043
Size: 15 91 44 336 349 576 349 2154
Cuts at: 4043 4415 4463 5489 6237 6651 6899 6934 129
Size: 372 48 1026 748 414 248 35 2416
Fragments arranged by size:
2416 2154 1026 748 576 414 372 349
349 336 248 91 48 44 35 15
BanII G_rGCy'C
Cuts at: 163 209 1100 2856 2915 3475 3531 3597 3603
Size: 46 891 1756 59 560 56 66 6
Cuts at: 3603 3785 4137 4737 4806 5706 5970 6235 7265
Size: 182 352 600 69 900 264 265 1030
Cuts at: 7265 163
Size: 2119
Fragments arranged by size:
2119 1756 1030 900 891 600 560 352
265 264 182 69 66 59 56 46
6
BbeI G_GCGC'C
Cuts at: 148 968 1544 4047 4419 4467 5493 6655 6903
Size: 820 576 2503 372 48 1026 1162 248
Cuts at: 6903 148
Size: 2466
Fragments arranged by size:
2503 2466 1162 1026 820 576 372 248
48
BbsI GAAGACnn'nnnn_
Cuts at: 724 3260 3587 3829 3958 6102 724
Size: 2536 327 242 129 2144 3843
Fragments arranged by size:
3843 2536 2144 327 242 129
BbvCI CC'TCA_GC
Cuts at: 3164 3329 3871 4310 5342 5759 3164
Size: 165 542 439 1032 417 6626
Fragments arranged by size:
6626 1032 542 439 417 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1875 1909 1875
Size: 34 9187
BciVI GTATCCnnnnn_n'
Cuts at: 2671 7119 8175 9221 2671
Size: 4448 1056 1046 2671
Fragments arranged by size:
4448 2671 1056 1046
BclI T'GATC_A
Cuts at: 6741 6741
Size: 9221
BglI GCCn_nnn'nGGC
Cuts at: 153 204 760 2956 3970 4306 4480 4810 5330
Size: 51 556 2196 1014 336 174 330 520
Cuts at: 5330 153
Size: 4044
Fragments arranged by size:
4044 2196 1014 556 520 336 330 174
51
BglII A'GATC_T
Cuts at: 1580 2039 2582 5291 6736 8773 1580
Size: 459 543 2709 1445 2037 2028
Fragments arranged by size:
2709 2037 2028 1445 543 459
BlpI GC'TnA_GC
Cuts at: 3271 4210 4814 4831 5606 6333 3271
Size: 939 604 17 775 727 6159
Fragments arranged by size:
6159 939 775 727 604 17
Bme1580I G_kGCm'C
Cuts at: 134 163 170 209 238 2856 3531 3597 3603
Size: 29 7 39 29 2618 675 66 6
Cuts at: 3603 4104 4440 4737 4755 4806 5706 5712 6846
Size: 501 336 297 18 51 900 6 1134
Cuts at: 6846 6939 8063 134
Size: 93 1124 1292
Fragments arranged by size:
2618 1292 1134 1124 900 675 501 336
297 93 66 51 39 29 29 18
7 6 6
BmgBI CAC'GTC
Cuts at: 4021 4021
Size: 9221
BmrI ACTGGGnnnn_n'
Cuts at: 157 1357 2294 6433 6514 6849 8993 157
Size: 1200 937 4139 81 335 2144 385
Fragments arranged by size:
4139 2144 1200 937 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1090 1119 1393 5313 7588 1090
Size: 29 274 3920 2275 2723
Fragments arranged by size:
3920 2723 2275 274 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 728 853 1009 1085 1198 1234 1471 1690
Size: 9 125 156 76 113 36 237 219
Cuts at: 1690 2371 2416 2899 3061 3082 3214 3460 3682
Size: 681 45 483 162 21 132 246 222
Cuts at: 3682 3745 3766 3850 3937 3988 4072 4168 4357
Size: 63 21 84 87 51 84 96 189
Cuts at: 4357 4462 4555 4609 4639 4777 4807 4840 4954
Size: 105 93 54 30 138 30 33 114
Cuts at: 4954 4996 5167 5179 5356 5485 5503 5536 5833
Size: 42 171 12 177 129 18 33 297
Cuts at: 5833 5917 5959 719
Size: 84 42 3981
Fragments arranged by size:
3981 681 483 297 246 237 222 219
189 177 171 162 156 138 132 129
125 114 113 105 96 93 87 84
84 84 76 63 54 51 45 42
42 36 33 33 30 30 21 21
18 12 9
Bpu10I CC'TnA_GC
Cuts at: 2263 3164 3329 3871 4310 5342 5446 5759 5969
Size: 901 165 542 439 1032 104 313 210
Cuts at: 5969 2263
Size: 5515
Fragments arranged by size:
5515 1032 901 542 439 313 210 165
104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2746 3178 3614 4981 5311 5758 6397 7276 7990
Size: 432 436 1367 330 447 639 879 714
Cuts at: 7990 8288 2746
Size: 298 3679
Fragments arranged by size:
3679 1367 879 714 639 447 436 432
330 298
BsaI GGTCTCn'nnnn_
Cuts at: 970 1242 2621 4255 4513 5773 8925 970
Size: 272 1379 1634 258 1260 3152 1266
Fragments arranged by size:
3152 1634 1379 1266 1260 272 258
BsaAI yAC'GTr
Cuts at: 515 627 759 4777 6574 515
Size: 112 132 4018 1797 3162
Fragments arranged by size:
4018 3162 1797 132 112
BsaBI GATnn'nnATC
Cuts at: 6 6735 8772 6
Size: 6729 2037 455
Fragments arranged by size:
6729 2037 455
BsaHI Gr'CG_yC
Cuts at: 145 267 965 1541 4044 4416 4464 4574 4847
Size: 122 698 576 2503 372 48 110 273
Cuts at: 4847 5490 5679 6652 6900 7602 9098 145
Size: 643 189 973 248 702 1496 268
Fragments arranged by size:
2503 1496 973 702 698 643 576 372
273 268 248 189 122 110 48
BsaWI w'CCGG_w
Cuts at: 1112 2231 3785 6410 6931 7609 8020 8167 8441
Size: 1119 1554 2625 521 678 411 147 274
Cuts at: 8441 1112
Size: 1892
Fragments arranged by size:
2625 1892 1554 1119 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 532 892 922 1792 1822 3073 3103 3772
Size: 30 360 30 870 30 1251 30 669
Cuts at: 3772 3802 4141 4171 502
Size: 30 339 30 5552
Fragments arranged by size:
5552 1251 870 669 360 339 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 349 897 1078 1081 1693 1813 2635 2956
Size: 3 548 181 3 612 120 822 321
Cuts at: 2956 3016 3094 3097 3100 3181 3346 3349 3352
Size: 60 78 3 3 81 165 3 3
Cuts at: 3352 3433 3532 3562 3565 3733 3847 3850 4273
Size: 81 99 30 3 168 114 3 423
Cuts at: 4273 4354 4357 4438 4441 4468 4672 4774 4777
Size: 81 3 81 3 27 204 102 3
Cuts at: 4777 4795 5116 5176 5218 5281 5302 5482 5533
Size: 18 321 60 42 63 21 180 51
Cuts at: 5533 5536 5704 5776 5872 5875 5956 5965 5968
Size: 3 168 72 96 3 81 9 3
Cuts at: 5968 6136 6157 346
Size: 168 21 3410
Fragments arranged by size:
3410 822 612 548 423 321 321 204
181 180 168 168 168 165 120 114
102 99 96 81 81 81 81 81
78 72 63 60 60 51 42 30
27 21 21 18 9 3 3 3
3 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 179 208 712 1053 2596 2609 3530 3534 3602
Size: 29 504 341 1543 13 921 4 68
Cuts at: 3602 4226 4394 4496 5399 5698 6646 7404 8069
Size: 624 168 102 903 299 948 758 665
Cuts at: 8069 8477 179
Size: 408 923
Fragments arranged by size:
1543 948 923 921 903 758 665 624
504 408 341 299 168 102 68 29
13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 678 1047 1304 1432 2145 2893 3011 3111
Size: 221 369 257 128 713 748 118 100
Cuts at: 3111 3883 4001 4093 4216 5008 457
Size: 772 118 92 123 792 4670
Fragments arranged by size:
4670 792 772 748 713 369 257 221
128 123 118 118 100 92
BsiEI CG_ry'CG
Cuts at: 104 1762 1885 4955 5979 6023 6763 6809 8039
Size: 1658 123 3070 1024 44 740 46 1230
Cuts at: 8039 104
Size: 1286
Fragments arranged by size:
3070 1658 1286 1230 1024 740 123 46
44
BsiHKAI G_wGCw'C
Cuts at: 2020 2915 3475 3785 4615 4920 5388 5849 6235
Size: 895 560 310 830 305 468 461 386
Cuts at: 6235 7013 7203 8063 2020
Size: 778 190 860 3178
Fragments arranged by size:
3178 895 860 830 778 560 468 461
386 310 305 190
BsmBI CGTCTCn'nnnn_
Cuts at: 274 773 1165 2658 2998 274
Size: 499 392 1493 340 6497
Fragments arranged by size:
6497 1493 499 392 340
Bsp1286I G_dGCh'C
Cuts at: 134 163 170 209 238 1100 2020 2856 2915
Size: 29 7 39 29 862 920 836 59
Cuts at: 2915 3475 3531 3597 3603 3785 4104 4137 4440
Size: 560 56 66 6 182 319 33 303
Cuts at: 4440 4615 4737 4755 4806 4920 5388 5706 5712
Size: 175 122 18 51 114 468 318 6
Cuts at: 5712 5849 5970 6235 6846 6939 7013 7203 7265
Size: 137 121 265 611 93 74 190 62
Cuts at: 7265 8063 134
Size: 798 1292
Fragments arranged by size:
1292 920 862 836 798 611 560 468
319 318 303 265 190 182 175 137
122 121 114 93 74 66 62 59
56 51 39 33 29 29 18 7
6 6
BspEI T'CCGG_A
Cuts at: 2231 2231
Size: 9221
BspHI T'CATG_A
Cuts at: 7653 7653
Size: 9221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 237 457 992 1739 1901 2808 3248 5027 5565
Size: 220 535 747 162 907 440 1779 538
Cuts at: 5565 6271 6485 6787 7168 237
Size: 706 214 302 381 2290
Fragments arranged by size:
2290 1779 907 747 706 538 535 440
381 302 220 214 162
BsrBI CCG'CTC
Cuts at: 50 362 489 837 1155 1758 3282 4108 4539
Size: 312 127 348 318 603 1524 826 431
Cuts at: 4539 4698 4791 5094 5106 5636 5865 7513 7567
Size: 159 93 303 12 530 229 1648 54
Cuts at: 7567 9051 9216 50
Size: 1484 165 55
Fragments arranged by size:
1648 1524 1484 826 603 530 431 348
318 312 303 229 165 159 127 93
55 54 12
BsrDI GCAATG_nn'
Cuts at: 885 1262 5726 7133 885
Size: 377 4464 1407 2973
Fragments arranged by size:
4464 2973 1407 377
BsrFI r'CCGG_y
Cuts at: 1112 1761 2024 4550 5188 5974 6059 7219 7400
Size: 649 263 2526 638 786 85 1160 181
Cuts at: 7400 1112
Size: 2933
Fragments arranged by size:
2933 2526 1160 786 649 638 263 181
85
BssHII G'CGCG_C
Cuts at: 2946 2946
Size: 9221
BssSI C'ACGA_G
Cuts at: 271 610 1207 2329 3850 4103 7492 8200 271
Size: 339 597 1122 1521 253 3389 708 1292
Fragments arranged by size:
3389 1521 1292 1122 708 597 339 253
BstAPI GCAn_nnn'nTGC
Cuts at: 2871 3186 4071 6598 2871
Size: 315 885 2527 5494
Fragments arranged by size:
5494 2527 885 315
BstBI TT'CG_AA
Cuts at: 7582 7582
Size: 9221
BstEII G'GTnAC_C
Cuts at: 2637 5440 2637
Size: 2803 6418
BstXI CCAn_nnnn'nTGG
Cuts at: 4587 4587
Size: 9221
BstYI r'GATC_y
Cuts at: 1 392 1580 2039 2110 2468 2582 5291 5660
Size: 391 1188 459 71 358 114 2709 369
Cuts at: 5660 6246 6713 6736 7071 7317 7623 7635 7721
Size: 586 467 23 335 246 306 12 86
Cuts at: 7721 8773 1
Size: 1052 449
Fragments arranged by size:
2709 1188 1052 586 467 459 449 391
369 358 335 306 246 114 86 71
23 12
Bsu36I CC'TnA_GG
Cuts at: 2728 6188 2728
Size: 3460 5761
BtgI C'CryG_G
Cuts at: 107 201 382 2356 3882 4301 4523 4580 5126
Size: 94 181 1974 1526 419 222 57 546
Cuts at: 5126 107
Size: 4202
Fragments arranged by size:
4202 1974 1526 546 419 222 181 94
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1551 7171 7352 7515 1551
Size: 5620 181 163 3257
Fragments arranged by size:
5620 3257 181 163
BtsI GCAGTG_nn'
Cuts at: 641 2785 2809 3866 4688 4825 6464 641
Size: 2144 24 1057 822 137 1639 3398
Fragments arranged by size:
3398 2144 1639 1057 822 137 24
DraI TTT'AAA
Cuts at: 6220 6220
Size: 9221
DraIII CAC_nnn'GTG
Cuts at: 615 2206 615
Size: 1591 7630
DrdI GACnn_nn'nnGTC
Cuts at: 1179 2635 2650 6927 8271 1179
Size: 1456 15 4277 1344 2129
Fragments arranged by size:
4277 2129 1456 1344 15
EaeI y'GGCC_r
Cuts at: 437 1146 1398 1460 1759 1763 1863 1882 2208
Size: 709 252 62 299 4 100 19 326
Cuts at: 2208 2276 3305 3569 3641 3677 3885 4952 5063
Size: 68 1029 264 72 36 208 1067 111
Cuts at: 5063 5123 5190 5369 5432 5976 6020 6806 6980
Size: 60 67 179 63 544 44 786 174
Cuts at: 6980 7371 7398 8743 437
Size: 391 27 1345 915
Fragments arranged by size:
1345 1067 1029 915 786 709 544 391
326 299 264 252 208 179 174 111
100 72 68 67 63 62 60 44
36 27 19 4
EagI C'GGCC_G
Cuts at: 1759 1882 4952 5976 6020 6806 1759
Size: 123 3070 1024 44 786 4174
Fragments arranged by size:
4174 3070 1024 786 123 44
EarI CTCTTCn'nnn_
Cuts at: 1168 1267 1489 1651 2329 2446 2612 2933 3112
Size: 99 222 162 678 117 166 321 179
Cuts at: 3112 3227 5039 5504 7244 7454 1168
Size: 115 1812 465 1740 210 2935
Fragments arranged by size:
2935 1812 1740 678 465 321 222 210
179 166 162 117 115 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 153 355 370 997 2132 3706 3922 5067 7429
Size: 202 15 627 1135 1574 216 1145 2362
Cuts at: 7429 8171 8317 9031 153
Size: 742 146 714 343
Fragments arranged by size:
2362 1574 1145 1135 742 714 627 343
216 202 146 15
EcoICRI GAG'CTC
Cuts at: 2913 3473 3783 6233 2913
Size: 560 310 2450 5901
Fragments arranged by size:
5901 2450 560 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 728 832 853 1009 1085 1198 1234 1471
Size: 9 104 21 156 76 113 36 237
Cuts at: 1471 1690 1896 2371 2416 2653 2899 3061 3082
Size: 219 206 475 45 237 246 162 21
Cuts at: 3082 3214 3460 3469 3682 3745 3766 3838 3850
Size: 132 246 9 213 63 21 72 12
Cuts at: 3850 3937 3988 4072 4168 4357 4462 4555 4609
Size: 87 51 84 96 189 105 93 54
Cuts at: 4609 4639 4777 4807 4840 4954 4996 5167 5179
Size: 30 138 30 33 114 42 171 12
Cuts at: 5179 5188 5356 5485 5503 5536 5833 5917 5947
Size: 9 168 129 18 33 297 84 30
Cuts at: 5947 5959 7046 7478 7831 8493 719
Size: 12 1087 432 353 662 1447
Fragments arranged by size:
1447 1087 662 475 432 353 297 246
246 237 237 219 213 206 189 171
168 162 156 138 132 129 114 113
105 104 96 93 87 84 84 76
72 63 54 51 45 42 36 33
33 30 30 30 21 21 21 18
12 12 12 9 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 457 1462 2443 3412 3622 3643 3715 5449 5686
Size: 1005 981 969 210 21 72 1734 237
Cuts at: 5686 457
Size: 3992
Fragments arranged by size:
3992 1734 1005 981 969 237 210 72
21
EcoO109I rG'GnC_Cy
Cuts at: 647 1191 1455 2391 2601 2693 3237 3593 3657
Size: 544 264 936 210 92 544 356 64
Cuts at: 3657 3708 3891 4286 4488 4734 4803 5331 5702
Size: 51 183 395 202 246 69 528 371
Cuts at: 5702 6194 6360 647
Size: 492 166 3508
Fragments arranged by size:
3508 936 544 544 528 492 395 371
356 264 246 210 202 183 166 92
69 64 51
EcoRI G'AATT_C
Cuts at: 3261 6225 3261
Size: 2964 6257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2604 7240 2604
Size: 4636 4585
FseI GG_CCGG'CC
Cuts at: 1765 5978 1765
Size: 4213 5008
FspI TGC'GCA
Cuts at: 5815 7002 5815
Size: 1187 8034
HaeII r_GCGC'y
Cuts at: 148 316 609 968 1064 1482 1544 2542 2709
Size: 168 293 359 96 418 62 998 167
Cuts at: 2709 2726 2897 3705 3733 3882 4047 4168 4419
Size: 17 171 808 28 149 165 121 251
Cuts at: 4419 4467 4503 5325 5493 6655 6903 8133 8904
Size: 48 36 822 168 1162 248 1230 771
Cuts at: 8904 148
Size: 465
Fragments arranged by size:
1230 1162 998 822 808 771 465 418
359 293 251 248 171 168 168 167
165 149 121 96 62 48 36 28
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 892 924 2453 2485 3301 3333 3649
Size: 32 193 32 1529 32 816 32 316
Cuts at: 3649 3681 3772 3804 4005 4037 4141 4165 4173
Size: 32 91 32 201 32 104 24 8
Cuts at: 4173 4197 4512 4544 5777 5809 5944 5976 7063
Size: 24 315 32 1233 32 135 32 1087
Cuts at: 7063 7093 7095 7125 667
Size: 30 2 30 2763
Fragments arranged by size:
2763 1529 1233 1087 816 316 315 201
193 135 104 91 32 32 32 32
32 32 32 32 32 32 30 30
24 24 8 2
HincII GTy'rAC
Cuts at: 1941 6260 1941
Size: 4319 4902
KasI G'GCGC_C
Cuts at: 144 964 1540 4043 4415 4463 5489 6651 6899
Size: 820 576 2503 372 48 1026 1162 248
Cuts at: 6899 144
Size: 2466
Fragments arranged by size:
2503 2466 1162 1026 820 576 372 248
48
KpnI G_GTAC'C
Cuts at: 6241 6241
Size: 9221
MfeI C'AATT_G
Cuts at: 2965 2965
Size: 9221
MluI A'CGCG_T
Cuts at: 8654 8654
Size: 9221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5483 6138 6161 7980 8164 5483
Size: 655 23 1819 184 6540
Fragments arranged by size:
6540 1819 655 184 23
MscI TGG'CCA
Cuts at: 1462 2278 3571 3679 5065 5434 6982 1462
Size: 816 1293 108 1386 369 1548 3701
Fragments arranged by size:
3701 1548 1386 1293 816 369 108
MslI CAynn'nnrTG
Cuts at: 127 1222 1663 5012 7210 7337 127
Size: 1095 441 3349 2198 127 2011
Fragments arranged by size:
3349 2198 2011 1095 441 127
MspA1I CmG'CkG
Cuts at: 203 384 843 1050 1318 1490 1590 1881 2312
Size: 181 459 207 268 172 100 291 431
Cuts at: 2312 2358 2613 2668 2742 2934 3192 3328 3510
Size: 46 255 55 74 192 258 136 182
Cuts at: 3510 3534 3606 3915 4038 4062 4230 4335 4351
Size: 24 72 309 123 24 168 105 16
Cuts at: 4351 4599 4669 4951 5061 5073 5481 5605 5649
Size: 248 70 282 110 12 408 124 44
Cuts at: 5649 5895 5925 5937 6332 6646 7006 7790 8035
Size: 246 30 12 395 314 360 784 245
Cuts at: 8035 203
Size: 1389
Fragments arranged by size:
1389 784 459 431 408 395 360 314
309 291 282 268 258 255 248 246
245 207 192 182 181 172 168 136
124 123 110 105 100 74 72 70
55 46 44 30 24 24 16 12
12
NaeI GCC'GGC
Cuts at: 1763 4552 5190 5976 7402 1763
Size: 2789 638 786 1426 3582
Fragments arranged by size:
3582 2789 1426 786 638
NarI GG'CG_CC
Cuts at: 145 965 1541 4044 4416 4464 5490 6652 6900
Size: 820 576 2503 372 48 1026 1162 248
Cuts at: 6900 145
Size: 2466
Fragments arranged by size:
2503 2466 1162 1026 820 576 372 248
48
NcoI C'CATG_G
Cuts at: 107 4301 4580 107
Size: 4194 279 4748
Fragments arranged by size:
4748 4194 279
NgoMIV G'CCGG_C
Cuts at: 1761 4550 5188 5974 7400 1761
Size: 2789 638 786 1426 3582
Fragments arranged by size:
3582 2789 1426 786 638
NspI r_CATG'y
Cuts at: 663 6274 6774 8377 663
Size: 5611 500 1603 1507
Fragments arranged by size:
5611 1603 1507 500
PasI CC'CwG_GG
Cuts at: 3894 3894
Size: 9221
PciI A'CATG_T
Cuts at: 8373 8373
Size: 9221
PflMI CCAn_nnn'nTGG
Cuts at: 1053 1958 2206 2541 3438 3894 4503 4650 1053
Size: 905 248 335 897 456 609 147 5624
Fragments arranged by size:
5624 905 897 609 456 335 248 147
PfoI T'CCnGG_A
Cuts at: 482 1166 1559 2393 2444 3248 5663 5885 482
Size: 684 393 834 51 804 2415 222 3818
Fragments arranged by size:
3818 2415 834 804 684 393 222 51
PmeI GTTT'AAAC
Cuts at: 6220 6220
Size: 9221
PmlI CAC'GTG
Cuts at: 627 759 4777 627
Size: 132 4018 5071
Fragments arranged by size:
5071 4018 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1792 1824 7667 7699 1792
Size: 32 5843 32 3314
Fragments arranged by size:
5843 3314 32 32
PpuMI rG'GwC_Cy
Cuts at: 1191 2391 2601 3237 3657 3708 4286 6194 1191
Size: 1200 210 636 420 51 578 1908 4218
Fragments arranged by size:
4218 1908 1200 636 578 420 210 51
PspOMI G'GGCC_C
Cuts at: 159 205 2852 3527 3593 3599 4733 4802 5702
Size: 46 2647 675 66 6 1134 69 900
Cuts at: 5702 159
Size: 3678
Fragments arranged by size:
3678 2647 1134 900 675 69 66 46
6
PspXI vC'TCGA_Gb
Cuts at: 1093 1093
Size: 9221
PstI C_TGCA'G
Cuts at: 668 1640 1736 2118 2819 2846 2867 3191 3485
Size: 972 96 382 701 27 21 324 294
Cuts at: 3485 3560 4013 4067 4130 4256 4382 4559 5024
Size: 75 453 54 63 126 126 177 465
Cuts at: 5024 5360 5576 5993 6268 6953 668
Size: 336 216 417 275 685 2936
Fragments arranged by size:
2936 972 701 685 465 453 417 382
336 324 294 275 216 177 126 126
96 75 63 54 27 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9221
PvuII CAG'CTG
Cuts at: 843 1050 1318 1590 2613 2668 2934 3192 3328
Size: 207 268 272 1023 55 266 258 136
Cuts at: 3328 3534 3606 3915 4062 4230 4335 4351 4669
Size: 206 72 309 147 168 105 16 318
Cuts at: 4669 5061 5073 5481 5605 5649 5895 5925 5937
Size: 392 12 408 124 44 246 30 12
Cuts at: 5937 6646 7006 843
Size: 709 360 3058
Fragments arranged by size:
3058 1023 709 408 392 360 318 309
272 268 266 258 246 207 206 168
147 136 124 105 72 55 44 30
16 12 12
RsrII CG'GwC_CG
Cuts at: 5379 7416 5379
Size: 2037 7184
SacI G_AGCT'C
Cuts at: 2915 3475 3785 6235 2915
Size: 560 310 2450 5901
Fragments arranged by size:
5901 2450 560 310
SacII CC_GC'GG
Cuts at: 204 385 2359 204
Size: 181 1974 7066
Fragments arranged by size:
7066 1974 181
SalI G'TCGA_C
Cuts at: 1939 6258 1939
Size: 4319 4902
SanDI GG'GwC_CC
Cuts at: 4286 6194 4286
Size: 1908 7313
SapI GCTCTTCn'nnn_
Cuts at: 1651 5504 7244 7454 1651
Size: 3853 1740 210 3418
Fragments arranged by size:
3853 3418 1740 210
SbfI CC_TGCA'GG
Cuts at: 5024 5576 6268 5024
Size: 552 692 7977
Fragments arranged by size:
7977 692 552
ScaI AGT'ACT
Cuts at: 8738 8738
Size: 9221
SexAI A'CCwGG_T
Cuts at: 2202 2202
Size: 9221
SfcI C'TryA_G
Cuts at: 33 664 1636 1732 2114 2815 2842 2863 2881
Size: 631 972 96 382 701 27 21 18
Cuts at: 2881 3124 3187 3481 3556 4009 4063 4126 4252
Size: 243 63 294 75 453 54 63 126
Cuts at: 4252 4378 4555 5020 5356 5572 5989 6264 6949
Size: 126 177 465 336 216 417 275 685
Cuts at: 6949 7917 8108 33
Size: 968 191 1146
Fragments arranged by size:
1146 972 968 701 685 631 465 453
417 382 336 294 275 243 216 191
177 126 126 96 75 63 63 54
27 21 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 3970 3970
Size: 9221
SfoI GGC'GCC
Cuts at: 146 966 1542 4045 4417 4465 5491 6653 6901
Size: 820 576 2503 372 48 1026 1162 248
Cuts at: 6901 146
Size: 2466
Fragments arranged by size:
2503 2466 1162 1026 820 576 372 248
48
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9221
SmaI CCC'GGG
Cuts at: 135 2674 3598 4291 4477 5284 5965 6243 135
Size: 2539 924 693 186 807 681 278 3113
Fragments arranged by size:
3113 2539 924 807 693 681 278 186
SmlI C'TyrA_G
Cuts at: 1093 2761 3157 3629 4996 5326 5737 6376 7291
Size: 1668 396 472 1367 330 411 639 915
Cuts at: 7291 8005 8267 1093
Size: 714 262 2047
Fragments arranged by size:
2047 1668 1367 915 714 639 472 411
396 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9221
SphI G_CATG'C
Cuts at: 663 6274 6774 663
Size: 5611 500 3110
Fragments arranged by size:
5611 3110 500
SrfI GCCC'GGGC
Cuts at: 135 3598 135
Size: 3463 5758
StuI AGG'CCT
Cuts at: 463 871 2518 463
Size: 408 1647 7166
Fragments arranged by size:
7166 1647 408
StyI C'CwwG_G
Cuts at: 107 1016 1463 1517 1536 2082 2492 2798 3686
Size: 909 447 54 19 546 410 306 888
Cuts at: 3686 4280 4301 4580 5624 6355 6709 107
Size: 594 21 279 1044 731 354 2619
Fragments arranged by size:
2619 1044 909 888 731 594 546 447
410 354 306 279 54 21 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 267 456 655 2493 5674 6804 6937 7396 7605
Size: 189 199 1838 3181 1130 133 459 209
Cuts at: 7605 267
Size: 1883
Fragments arranged by size:
3181 1883 1838 1130 459 209 199 189
133
TatI w'GTAC_w
Cuts at: 4671 8736 4671
Size: 4065 5156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1337 1369 1786 1818 3073 3105 5795 5827 8738
Size: 32 417 32 1255 32 2690 32 2911
Cuts at: 8738 8770 1337
Size: 32 1788
Fragments arranged by size:
2911 2690 1788 1255 417 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 123 1993 7018 123
Size: 1870 5025 2326
Fragments arranged by size:
5025 2326 1870
XbaI T'CTAG_A
Cuts at: 6252 6252
Size: 9221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1230 1665 2499 4272 4584 4971 6716 1230
Size: 435 834 1773 312 387 1745 3735
Fragments arranged by size:
3735 1773 1745 834 435 387 312
XhoI C'TCGA_G
Cuts at: 1093 1093
Size: 9221
XmaI C'CCGG_G
Cuts at: 133 2672 3596 4289 4475 5282 5963 6241 133
Size: 2539 924 693 186 807 681 278 3113
Fragments arranged by size:
3113 2539 924 807 693 681 278 186
XmnI GAAnn'nnTTC
Cuts at: 1597 1597
Size: 9221
ZraI GAC'GTC
Cuts at: 4848 4848
Size: 9221
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsrGI BstZ17I ClaI CspCI EcoRV FspAI
HindIII HpaI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PsrI SgrAI SnaBI SspI SwaI