Restriction map for
pF1KA2022
(Circular) (Six-base) MAPSORT of: pF1KA2022.seq Check: 8139 from: 1 to: 7661
pF1KA2022 7661 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1986 1986
Size: 7661
AccI GT'mk_AC
Cuts at: 4699 4699
Size: 7661
Acc65I G'GTAC_C
Cuts at: 4677 4677
Size: 7661
AclI AA'CG_TT
Cuts at: 7501 7501
Size: 7661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1192 1376 2152 2589 2834 5486 5918 6271
Size: 203 184 776 437 245 2652 432 353
Cuts at: 6271 6933 989
Size: 662 1717
Fragments arranged by size:
2652 1717 776 662 437 432 353 245
203 184
AflII C'TTAA_G
Cuts at: 1369 3132 1369
Size: 1763 5898
AflIII A'CryG_T
Cuts at: 634 989 5013 6813 7094 634
Size: 355 4024 1800 281 1201
Fragments arranged by size:
4024 1800 1201 355 281
AgeI A'CCGG_T
Cuts at: 258 258
Size: 7661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1118 1118
Size: 7661
AlwNI CAG_nnn'CTG
Cuts at: 646 2197 4966 6404 646
Size: 1551 2769 1438 1903
Fragments arranged by size:
2769 1903 1551 1438
ApaI G_GGCC'C
Cuts at: 1170 1170
Size: 7661
ApaLI G'TGCA_C
Cuts at: 6499 6499
Size: 7661
ApoI r'AATT_y
Cuts at: 864 1040 1812 2042 2269 2431 2803 2851 3292
Size: 176 772 230 227 162 372 48 441
Cuts at: 3292 4042 4665 7014 7270 864
Size: 750 623 2349 256 1255
Fragments arranged by size:
2349 1255 772 750 623 441 372 256
230 227 176 162 48
AseI AT'TA_AT
Cuts at: 20 167 668 1742 20
Size: 147 501 1074 5939
Fragments arranged by size:
5939 1074 501 147
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7661
AvaI C'yCGr_G
Cuts at: 1499 2538 4681 1499
Size: 1039 2143 4479
Fragments arranged by size:
4479 2143 1039
AvrII C'CTAG_G
Cuts at: 720 720
Size: 7661
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2577 2610 3441 3474 2577
Size: 33 831 33 6764
Fragments arranged by size:
6764 831 33 33
BamHI G'GATC_C
Cuts at: 792 4155 4686 792
Size: 3363 531 3767
Fragments arranged by size:
3767 3363 531
BanI G'GyrC_C
Cuts at: 1851 2794 4003 4677 5091 5339 5374 1851
Size: 943 1209 674 414 248 35 4138
Fragments arranged by size:
4138 1209 943 674 414 248 35
BanII G_rGCy'C
Cuts at: 337 1170 1715 2350 4127 4675 5705 337
Size: 833 545 635 1777 548 1030 2293
Fragments arranged by size:
2293 1777 1030 833 635 548 545
BbeI G_GCGC'C
Cuts at: 5095 5343 5095
Size: 248 7413
BbsI GAAGACnn'nnnn_
Cuts at: 1286 3382 3880 1286
Size: 2096 498 5067
Fragments arranged by size:
5067 2096 498
BbvCI CC'TCA_GC
Cuts at: 141 141
Size: 7661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 358 392 517 551 358
Size: 34 125 34 7468
Fragments arranged by size:
7468 125 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 296 3901 5559 6615 7661 296
Size: 3605 1658 1056 1046 296
Fragments arranged by size:
3605 1658 1056 1046 296
BclI T'GATC_A
Cuts at: 2079 5181 2079
Size: 3102 4559
BglI GCCn_nnn'nGGC
Cuts at: 1849 1849
Size: 7661
BglII A'GATC_T
Cuts at: 1448 3026 5176 7213 1448
Size: 1578 2150 2037 1896
Fragments arranged by size:
2150 2037 1896 1578
BlpI GC'TnA_GC
Cuts at: 4773 4773
Size: 7661
Bme1580I G_kGCm'C
Cuts at: 1170 4006 5286 5379 6503 1170
Size: 2836 1280 93 1124 2328
Fragments arranged by size:
2836 2328 1280 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3878 4873 4954 5289 7433 3878
Size: 995 81 335 2144 4106
Fragments arranged by size:
4106 2144 995 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6028 6028
Size: 7661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 1426 2603 2656 3968 4005 695
Size: 731 1177 53 1312 37 4351
Fragments arranged by size:
4351 1312 1177 731 53 37
Bpu10I CC'TnA_GC
Cuts at: 141 546 584 3203 141
Size: 405 38 2619 4599
Fragments arranged by size:
4599 2619 405 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2805 3099 4837 5716 6430 6728 2805
Size: 294 1738 879 714 298 3738
Fragments arranged by size:
3738 1738 879 714 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 268 301 7365 268
Size: 33 7064 564
Fragments arranged by size:
7064 564 33
BsaAI yAC'GTr
Cuts at: 5014 5014
Size: 7661
BsaBI GATnn'nnATC
Cuts at: 6 1050 5175 7212 6
Size: 1044 4125 2037 455
Fragments arranged by size:
4125 2037 1044 455
BsaHI Gr'CG_yC
Cuts at: 5092 5340 6042 7538 5092
Size: 248 702 1496 5215
Fragments arranged by size:
5215 1496 702 248
BsaWI w'CCGG_w
Cuts at: 258 3327 4850 5371 6049 6460 6607 6881 258
Size: 3069 1523 521 678 411 147 274 1038
Fragments arranged by size:
3069 1523 1038 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1410 1440 1891 1921 1410
Size: 30 451 30 7150
Fragments arranged by size:
7150 451 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1512 1515 1512
Size: 3 7658
BseYI C'CCAG_C
Cuts at: 1123 2314 3649 4219 5086 5844 6509 6917 1123
Size: 1191 1335 570 867 758 665 408 1867
Fragments arranged by size:
1867 1335 1191 867 758 665 570 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 659 4117 224
Size: 435 3458 3768
Fragments arranged by size:
3768 3458 435
BsiEI CG_ry'CG
Cuts at: 104 5203 5249 6479 104
Size: 5099 46 1230 1286
Fragments arranged by size:
5099 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2154 2258 2696 4148 4414 4675 5453 5643 6503
Size: 104 438 1452 266 261 778 190 860
Cuts at: 6503 2154
Size: 3312
Fragments arranged by size:
3312 1452 860 778 438 266 261 190
104
BsmI GAATG_Cn'
Cuts at: 402 402
Size: 7661
Bsp1286I G_dGCh'C
Cuts at: 337 1170 1715 2154 2258 2350 2696 4006 4127
Size: 833 545 439 104 92 346 1310 121
Cuts at: 4127 4148 4414 4675 5286 5379 5453 5643 5705
Size: 21 266 261 611 93 74 190 62
Cuts at: 5705 6503 337
Size: 798 1495
Fragments arranged by size:
1495 1310 833 798 611 545 439 346
266 261 190 121 104 93 92 74
62 21
BspHI T'CATG_A
Cuts at: 2436 4106 6093 2436
Size: 1670 1987 4004
Fragments arranged by size:
4004 1987 1670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1147 1986 3462 4711 4925 5227 5608 1147
Size: 839 1476 1249 214 302 381 3200
Fragments arranged by size:
3200 1476 1249 839 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5953 6007 7491 7656 50
Size: 5903 54 1484 165 55
Fragments arranged by size:
5903 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 215 419 3192 4073 4317 5573 215
Size: 204 2773 881 244 1256 2303
Fragments arranged by size:
2773 2303 1256 881 244 204
BsrFI r'CCGG_y
Cuts at: 258 5659 5840 258
Size: 5401 181 2079
Fragments arranged by size:
5401 2079 181
BssSI C'ACGA_G
Cuts at: 5932 6640 5932
Size: 708 6953
BstAPI GCAn_nnn'nTGC
Cuts at: 2449 2939 5038 2449
Size: 490 2099 5072
Fragments arranged by size:
5072 2099 490
BstBI TT'CG_AA
Cuts at: 6022 6022
Size: 7661
BstXI CCAn_nnnn'nTGG
Cuts at: 3901 3901
Size: 7661
BstYI r'GATC_y
Cuts at: 1 579 792 1448 3026 4155 4686 5153 5176
Size: 578 213 656 1578 1129 531 467 23
Cuts at: 5176 5511 5757 6063 6075 6161 7213 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1578 1129 1052 656 578 531 467 449
335 306 246 213 86 23 12
BtgI C'CryG_G
Cuts at: 107 2876 3834 4139 4149 107
Size: 2769 958 305 10 3619
Fragments arranged by size:
3619 2769 958 305 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3176 5611 5792 5955 3176
Size: 2435 181 163 4882
Fragments arranged by size:
4882 2435 181 163
BtsI GCAGTG_nn'
Cuts at: 1026 1188 1353 4904 1026
Size: 162 165 3551 3783
Fragments arranged by size:
3783 3551 165 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2910 2945 2910
Size: 35 7626
DraI TTT'AAA
Cuts at: 1752 2180 2274 2384 3500 4660 1752
Size: 428 94 110 1116 1160 4753
Fragments arranged by size:
4753 1160 1116 428 110 94
DrdI GACnn_nn'nnGTC
Cuts at: 5367 6711 5367
Size: 1344 6317
EaeI y'GGCC_r
Cuts at: 3831 5246 5420 5811 5838 7183 3831
Size: 1415 174 391 27 1345 4309
Fragments arranged by size:
4309 1415 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5246 5246
Size: 7661
EarI CTCTTCn'nnn_
Cuts at: 2490 3309 3684 4622 5684 5894 2490
Size: 819 375 938 1062 210 4257
Fragments arranged by size:
4257 1062 938 819 375 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3286 5869 6611 6757 7471 3286
Size: 2583 742 146 714 3476
Fragments arranged by size:
3476 2583 742 714 146
EcoICRI GAG'CTC
Cuts at: 4673 4673
Size: 7661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 989 1192 1376 1426 2152 2589 2603 2656
Size: 294 203 184 50 726 437 14 53
Cuts at: 2656 2834 3968 4005 5486 5918 6271 6933 695
Size: 178 1134 37 1481 432 353 662 1423
Fragments arranged by size:
1481 1423 1134 726 662 437 432 353
294 203 184 178 53 50 37 14
EcoNI CCTnn'n_nnAGG
Cuts at: 587 587
Size: 7661
EcoO109I rG'GnC_Cy
Cuts at: 1166 1167 1203 2194 2949 3762 4259 4473 4800
Size: 1 36 991 755 813 497 214 327
Cuts at: 4800 1166
Size: 4027
Fragments arranged by size:
4027 991 813 755 497 327 214 36
1
EcoRI G'AATT_C
Cuts at: 2803 4665 2803
Size: 1862 5799
EcoRV GAT'ATC
Cuts at: 3171 3171
Size: 7661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 557 874 1347 1956 5680 557
Size: 317 473 609 3724 2538
Fragments arranged by size:
3724 2538 609 473 317
FspI TGC'GCA
Cuts at: 5442 5442
Size: 7661
HaeII r_GCGC'y
Cuts at: 5095 5343 6573 7344 5095
Size: 248 1230 771 5412
Fragments arranged by size:
5412 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 991 1015 1023 1047 1582 1614 1809 1841 3915
Size: 24 8 24 535 32 195 32 2074
Cuts at: 3915 3947 4360 4392 4505 4537 5503 5533 5535
Size: 32 413 32 113 32 966 30 2
Cuts at: 5535 5565 991
Size: 30 3087
Fragments arranged by size:
3087 2074 966 535 413 195 113 32
32 32 32 32 30 30 24 24
8 2
HincII GTy'rAC
Cuts at: 957 1596 3578 4700 957
Size: 639 1982 1122 3918
Fragments arranged by size:
3918 1982 1122 639
HindIII A'AGCT_T
Cuts at: 851 1938 3135 851
Size: 1087 1197 5377
Fragments arranged by size:
5377 1197 1087
KasI G'GCGC_C
Cuts at: 5091 5339 5091
Size: 248 7413
KpnI G_GTAC'C
Cuts at: 4681 4681
Size: 7661
MfeI C'AATT_G
Cuts at: 2333 2333
Size: 7661
MluI A'CGCG_T
Cuts at: 7094 7094
Size: 7661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 517 3299 3918 4094 4595 6420 6604 517
Size: 2782 619 176 501 1825 184 1574
Fragments arranged by size:
2782 1825 1574 619 501 184 176
MscI TGG'CCA
Cuts at: 5422 5422
Size: 7661
MslI CAynn'nnrTG
Cuts at: 1771 2180 4529 5650 5777 1771
Size: 409 2349 1121 127 3655
Fragments arranged by size:
3655 2349 1121 409 127
MspA1I CmG'CkG
Cuts at: 702 4772 5086 5446 6230 6475 702
Size: 4070 314 360 784 245 1888
Fragments arranged by size:
4070 1888 784 360 314 245
NaeI GCC'GGC
Cuts at: 5842 5842
Size: 7661
NarI GG'CG_CC
Cuts at: 5092 5340 5092
Size: 248 7413
NcoI C'CATG_G
Cuts at: 107 2876 4139 4149 107
Size: 2769 1263 10 3619
Fragments arranged by size:
3619 2769 1263 10
NdeI CA'TA_TG
Cuts at: 1455 3524 1455
Size: 2069 5592
NgoMIV G'CCGG_C
Cuts at: 5840 5840
Size: 7661
NspI r_CATG'y
Cuts at: 376 501 993 1770 2483 4353 4714 5214 6817
Size: 125 492 777 713 1870 361 500 1603
Cuts at: 6817 376
Size: 1220
Fragments arranged by size:
1870 1603 1220 777 713 500 492 361
125
PciI A'CATG_T
Cuts at: 989 6813 989
Size: 5824 1837
PflMI CCAn_nnn'nTGG
Cuts at: 204 1348 204
Size: 1144 6517
PfoI T'CCnGG_A
Cuts at: 3263 3263
Size: 7661
PmeI GTTT'AAAC
Cuts at: 4660 4660
Size: 7661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3693 6107 6139 3661
Size: 32 2414 32 5183
Fragments arranged by size:
5183 2414 32 32
PpuMI rG'GwC_Cy
Cuts at: 1203 2949 3762 4259 1203
Size: 1746 813 497 4605
Fragments arranged by size:
4605 1746 813 497
PshAI GACnn'nnGTC
Cuts at: 1484 1484
Size: 7661
PsiI TTA'TAA
Cuts at: 4361 4361
Size: 7661
PspOMI G'GGCC_C
Cuts at: 1166 1166
Size: 7661
PstI C_TGCA'G
Cuts at: 495 2555 2723 3457 4708 5393 495
Size: 2060 168 734 1251 685 2763
Fragments arranged by size:
2763 2060 1251 734 685 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7661
PvuII CAG'CTG
Cuts at: 702 5086 5446 702
Size: 4384 360 2917
Fragments arranged by size:
4384 2917 360
RsrII CG'GwC_CG
Cuts at: 4429 5856 4429
Size: 1427 6234
SacI G_AGCT'C
Cuts at: 4675 4675
Size: 7661
SalI G'TCGA_C
Cuts at: 4698 4698
Size: 7661
SanDI GG'GwC_CC
Cuts at: 4259 4259
Size: 7661
SapI GCTCTTCn'nnn_
Cuts at: 2490 3684 5684 5894 2490
Size: 1194 2000 210 4257
Fragments arranged by size:
4257 2000 1194 210
SbfI CC_TGCA'GG
Cuts at: 495 4708 495
Size: 4213 3448
ScaI AGT'ACT
Cuts at: 7178 7178
Size: 7661
SexAI A'CCwGG_T
Cuts at: 1947 1947
Size: 7661
SfcI C'TryA_G
Cuts at: 33 491 1482 2551 2719 2760 3453 4704 5389
Size: 458 991 1069 168 41 693 1251 685
Cuts at: 5389 6357 6548 33
Size: 968 191 1146
Fragments arranged by size:
1251 1146 1069 991 968 693 685 458
191 168 41
SfoI GGC'GCC
Cuts at: 5093 5341 5093
Size: 248 7413
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7661
SgrAI Cr'CCGG_yG
Cuts at: 258 258
Size: 7661
SmaI CCC'GGG
Cuts at: 2540 4683 2540
Size: 2143 5518
SmlI C'TyrA_G
Cuts at: 1369 2820 3114 3132 4816 5731 6445 6707 1369
Size: 1451 294 18 1684 915 714 262 2323
Fragments arranged by size:
2323 1684 1451 915 714 294 262 18
SpeI A'CTAG_T
Cuts at: 67 2590 67
Size: 2523 5138
SphI G_CATG'C
Cuts at: 4714 5214 4714
Size: 500 7161
SspI AAT'ATT
Cuts at: 4214 4214
Size: 7661
StuI AGG'CCT
Cuts at: 431 2117 431
Size: 1686 5975
StyI C'CwwG_G
Cuts at: 107 566 720 2876 3959 4139 4149 4263 4469
Size: 459 154 2156 1083 180 10 114 206
Cuts at: 4469 4795 5149 107
Size: 326 354 2619
Fragments arranged by size:
2619 2156 1083 459 354 326 206 180
154 114 10
SwaI ATTT'AAAT
Cuts at: 2180 2180
Size: 7661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1456 2137 2812 3663 4446 5244 5377 5836 6045
Size: 681 675 851 783 798 133 459 209
Cuts at: 6045 1456
Size: 3072
Fragments arranged by size:
3072 851 798 783 681 675 459 209
133
TatI w'GTAC_w
Cuts at: 7176 7176
Size: 7661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1410 1442 3458 3490 7178 7210 1410
Size: 32 2016 32 3688 32 1861
Fragments arranged by size:
3688 2016 1861 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5458 5458
Size: 7661
XbaI T'CTAG_A
Cuts at: 228 4692 228
Size: 4464 3197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1537 3261 3484 4020 5156 1537
Size: 1724 223 536 1136 4042
Fragments arranged by size:
4042 1724 1136 536 223
XmaI C'CCGG_G
Cuts at: 2538 4681 2538
Size: 2143 5518
XmnI GAAnn'nnTTC
Cuts at: 1055 1864 2396 1055
Size: 809 532 6320
Fragments arranged by size:
6320 809 532
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI BfrBI BmgBI
BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII FseI FspAI HpaI NheI NotI
NruI NsiI PacI PasI PmlI PspXI PsrI SacII
SfiI SnaBI SrfI XhoI ZraI