Restriction map for
pF1KA2015
(Circular) (Six-base) MAPSORT of: pF1KA2015.seq Check: 7863 from: 1 to: 6089
pF1KA2015 6089 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 3127 3127
Size: 6089
Acc65I G'GTAC_C
Cuts at: 3105 3105
Size: 6089
AclI AA'CG_TT
Cuts at: 5929 5929
Size: 6089
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 977 1379 1915 2135 2375 3914 4346 5093
Size: 872 402 536 220 240 1539 432 747
Cuts at: 5093 5361 105
Size: 268 833
Fragments arranged by size:
1539 872 833 747 536 432 402 268
240 220
AfeI AGC'GCT
Cuts at: 262 262
Size: 6089
AflII C'TTAA_G
Cuts at: 2521 2521
Size: 6089
AflIII A'CryG_T
Cuts at: 797 3441 4545 5522 797
Size: 2644 1104 977 1364
Fragments arranged by size:
2644 1364 1104 977
AgeI A'CCGG_T
Cuts at: 4477 4477
Size: 6089
AlwNI CAG_nnn'CTG
Cuts at: 1844 3394 4961 1844
Size: 1550 1567 2972
Fragments arranged by size:
2972 1567 1550
ApaLI G'TGCA_C
Cuts at: 4859 4859
Size: 6089
ApoI r'AATT_y
Cuts at: 633 658 860 939 1086 1311 1975 2105 2131
Size: 25 202 79 147 225 664 130 26
Cuts at: 2131 2734 2785 3017 3093 5442 5698 633
Size: 603 51 232 76 2349 256 1024
Fragments arranged by size:
2349 1024 664 603 256 232 225 202
147 130 79 76 51 26 25
AseI AT'TA_AT
Cuts at: 20 1829 1894 2057 2152 2842 20
Size: 1809 65 163 95 690 3267
Fragments arranged by size:
3267 1809 690 163 95 65
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
AvaI C'yCGr_G
Cuts at: 1544 3109 1544
Size: 1565 4524
BamHI G'GATC_C
Cuts at: 3114 3114
Size: 6089
BanI G'GyrC_C
Cuts at: 478 3105 3519 3767 3802 478
Size: 2627 414 248 35 2765
Fragments arranged by size:
2765 2627 414 248 35
BanII G_rGCy'C
Cuts at: 160 3103 4133 160
Size: 2943 1030 2116
Fragments arranged by size:
2943 2116 1030
BbeI G_GCGC'C
Cuts at: 482 3523 3771 482
Size: 3041 248 2800
Fragments arranged by size:
3041 2800 248
BbsI GAAGACnn'nnnn_
Cuts at: 2381 2381
Size: 6089
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 805 839 805
Size: 34 6055
BciVI GTATCCnnnnn_n'
Cuts at: 3987 4748 6089 3987
Size: 761 1341 3987
Fragments arranged by size:
3987 1341 761
BclI T'GATC_A
Cuts at: 2870 3609 2870
Size: 739 5350
BfrBI ATG'CAT
Cuts at: 1680 2266 1680
Size: 586 5503
BglII A'GATC_T
Cuts at: 1866 3604 5641 1866
Size: 1738 2037 2314
Fragments arranged by size:
2314 2037 1738
BlpI GC'TnA_GC
Cuts at: 3201 3201
Size: 6089
Bme1580I G_kGCm'C
Cuts at: 339 3714 3807 4863 339
Size: 3375 93 1056 1565
Fragments arranged by size:
3375 1565 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 213 3301 3382 3717 5861 213
Size: 3088 81 335 2144 441
Fragments arranged by size:
3088 2144 441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1612 4456 1612
Size: 2844 3245
Bpu10I CC'TnA_GC
Cuts at: 154 154
Size: 6089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 776 1870 2500 3265 4144 4636 4934 776
Size: 1094 630 765 879 492 298 1931
Fragments arranged by size:
1931 1094 879 765 630 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5793 5793
Size: 6089
BsaAI yAC'GTr
Cuts at: 781 3442 781
Size: 2661 3428
BsaBI GATnn'nnATC
Cuts at: 6 391 3603 5640 6
Size: 385 3212 2037 455
Fragments arranged by size:
3212 2037 455 385
BsaHI Gr'CG_yC
Cuts at: 135 245 293 479 3520 3768 4470 5966 135
Size: 110 48 186 3041 248 702 1496 258
Fragments arranged by size:
3041 1496 702 258 248 186 110 48
BsaWI w'CCGG_w
Cuts at: 3278 3799 4477 4751 4898 5309 3278
Size: 521 678 274 147 411 4058
Fragments arranged by size:
4058 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1750 1750
Size: 6089
BseYI C'CCAG_C
Cuts at: 3514 4272 4849 5345 3514
Size: 758 577 496 4258
Fragments arranged by size:
4258 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 2620 355
Size: 2265 3824
BsiEI CG_ry'CG
Cuts at: 104 3631 3677 4885 104
Size: 3527 46 1208 1308
Fragments arranged by size:
3527 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 160 3103 3881 4071 4863 160
Size: 2943 778 190 792 1386
Fragments arranged by size:
2943 1386 792 778 190
BsmI GAATG_Cn'
Cuts at: 1305 1305
Size: 6089
BsmBI CGTCTCn'nnnn_
Cuts at: 126 126
Size: 6089
Bsp1286I G_dGCh'C
Cuts at: 160 339 3103 3714 3807 3881 4071 4133 4863
Size: 179 2764 611 93 74 190 62 730
Cuts at: 4863 160
Size: 1386
Fragments arranged by size:
2764 1386 730 611 190 179 93 74
62
BspEI T'CCGG_A
Cuts at: 5309 5309
Size: 6089
BspHI T'CATG_A
Cuts at: 5265 5265
Size: 6089
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 3139 3353 3655 4036 266
Size: 2873 214 302 381 2319
Fragments arranged by size:
2873 2319 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4381 4435 5919 6084 50
Size: 4331 54 1484 165 55
Fragments arranged by size:
4331 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1106 4001 1106
Size: 2895 3194
BsrFI r'CCGG_y
Cuts at: 4087 4268 4477 4087
Size: 181 209 5699
Fragments arranged by size:
5699 209 181
BsrGI T'GTAC_A
Cuts at: 432 1626 2457 432
Size: 1194 831 4064
Fragments arranged by size:
4064 1194 831
BssSI C'ACGA_G
Cuts at: 4360 4718 4360
Size: 358 5731
BstAPI GCAn_nnn'nTGC
Cuts at: 3466 3466
Size: 6089
BstBI TT'CG_AA
Cuts at: 900 4450 900
Size: 3550 2539
BstYI r'GATC_y
Cuts at: 1 218 1866 3114 3581 3604 3939 4185 5197
Size: 217 1648 1248 467 23 335 246 1012
Cuts at: 5197 5283 5295 5641 1
Size: 86 12 346 449
Fragments arranged by size:
1648 1248 1012 467 449 346 335 246
217 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1639 1639
Size: 6089
BtgI C'CryG_G
Cuts at: 161 248 339 2292 161
Size: 87 91 1953 3958
Fragments arranged by size:
3958 1953 91 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1090 4039 4220 4383 1090
Size: 2949 181 163 2796
Fragments arranged by size:
2949 2796 181 163
BtsI GCAGTG_nn'
Cuts at: 512 1388 2603 2816 2873 3332 512
Size: 876 1215 213 57 459 3269
Fragments arranged by size:
3269 1215 876 459 213 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1238 1273 1238
Size: 35 6054
DraI TTT'AAA
Cuts at: 1916 3088 1916
Size: 1172 4917
DrdI GACnn_nn'nnGTC
Cuts at: 3795 4653 3795
Size: 858 5231
EaeI y'GGCC_r
Cuts at: 342 804 2369 2408 3008 3674 3848 4239 4266
Size: 462 1565 39 600 666 174 391 27
Cuts at: 4266 5611 342
Size: 1345 820
Fragments arranged by size:
1565 1345 820 666 600 462 391 174
39 27
EagI C'GGCC_G
Cuts at: 3674 3674
Size: 6089
EarI CTCTTCn'nnn_
Cuts at: 124 440 792 2207 2756 2930 4112 4322 124
Size: 316 352 1415 549 174 1182 210 1891
Fragments arranged by size:
1891 1415 1182 549 352 316 210 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4297 4607 4753 5899 4297
Size: 310 146 1146 4487
Fragments arranged by size:
4487 1146 310 146
EcoICRI GAG'CTC
Cuts at: 158 3101 158
Size: 2943 3146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 105 977 1379 1915 2135 2375 3914 4346 5093
Size: 872 402 536 220 240 1539 432 747
Cuts at: 5093 5361 105
Size: 268 833
Fragments arranged by size:
1539 872 833 747 536 432 402 268
240 220
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 6089
EcoO109I rG'GnC_Cy
Cuts at: 3228 3228
Size: 6089
EcoRI G'AATT_C
Cuts at: 1311 2131 3017 3093 1311
Size: 820 886 76 4307
Fragments arranged by size:
4307 886 820 76
EcoRV GAT'ATC
Cuts at: 501 501
Size: 6089
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2814 3078 4108 2814
Size: 264 1030 4795
Fragments arranged by size:
4795 1030 264
FspI TGC'GCA
Cuts at: 3870 3870
Size: 6089
HaeII r_GCGC'y
Cuts at: 151 264 482 3523 3771 4793 5772 151
Size: 113 218 3041 248 1022 979 468
Fragments arranged by size:
3041 1022 979 468 248 218 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 371 403 1731 1763 2428 2460 3931 3961 3963
Size: 32 1328 32 665 32 1471 30 2
Cuts at: 3963 3993 371
Size: 30 2467
Fragments arranged by size:
2467 1471 1328 665 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 696 1750 3128 696
Size: 1054 1378 3657
Fragments arranged by size:
3657 1378 1054
KasI G'GCGC_C
Cuts at: 478 3519 3767 478
Size: 3041 248 2800
Fragments arranged by size:
3041 2800 248
KpnI G_GTAC'C
Cuts at: 3109 3109
Size: 6089
MfeI C'AATT_G
Cuts at: 2286 2286
Size: 6089
MluI A'CGCG_T
Cuts at: 5522 5522
Size: 6089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 876 2480 4760 4944 876
Size: 1604 2280 184 2021
Fragments arranged by size:
2280 2021 1604 184
MscI TGG'CCA
Cuts at: 806 2410 3850 806
Size: 1604 1440 3045
Fragments arranged by size:
3045 1604 1440
MslI CAynn'nnrTG
Cuts at: 344 1111 1685 4078 4205 344
Size: 767 574 2393 127 2228
Fragments arranged by size:
2393 2228 767 574 127
MspA1I CmG'CkG
Cuts at: 250 1632 2901 3200 3514 3874 4887 5132 250
Size: 1382 1269 299 314 360 1013 245 1207
Fragments arranged by size:
1382 1269 1207 1013 360 314 299 245
NaeI GCC'GGC
Cuts at: 4270 4270
Size: 6089
NarI GG'CG_CC
Cuts at: 479 3520 3768 479
Size: 3041 248 2800
Fragments arranged by size:
3041 2800 248
NcoI C'CATG_G
Cuts at: 161 339 2292 161
Size: 178 1953 3958
Fragments arranged by size:
3958 1953 178
NdeI CA'TA_TG
Cuts at: 650 2531 650
Size: 1881 4208
NgoMIV G'CCGG_C
Cuts at: 4268 4268
Size: 6089
NsiI A_TGCA'T
Cuts at: 1682 2268 1682
Size: 586 5503
NspI r_CATG'y
Cuts at: 691 801 3142 3642 4549 691
Size: 110 2341 500 907 2231
Fragments arranged by size:
2341 2231 907 500 110
PciI A'CATG_T
Cuts at: 797 4545 797
Size: 3748 2341
PfoI T'CCnGG_A
Cuts at: 281 467 637 281
Size: 186 170 5733
Fragments arranged by size:
5733 186 170
PmeI GTTT'AAAC
Cuts at: 3088 3088
Size: 6089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5228 5260 5228
Size: 32 6057
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2933 2965 2933
Size: 32 6057
PstI C_TGCA'G
Cuts at: 3136 3821 3136
Size: 685 5404
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6089
PvuII CAG'CTG
Cuts at: 1632 3514 3874 1632
Size: 1882 360 3847
Fragments arranged by size:
3847 1882 360
RsrII CG'GwC_CG
Cuts at: 4284 4284
Size: 6089
SacI G_AGCT'C
Cuts at: 160 3103 160
Size: 2943 3146
SacII CC_GC'GG
Cuts at: 251 251
Size: 6089
SalI G'TCGA_C
Cuts at: 3126 3126
Size: 6089
SapI GCTCTTCn'nnn_
Cuts at: 124 4112 4322 124
Size: 3988 210 1891
Fragments arranged by size:
3988 1891 210
SbfI CC_TGCA'GG
Cuts at: 3136 3136
Size: 6089
ScaI AGT'ACT
Cuts at: 5606 5606
Size: 6089
SfcI C'TryA_G
Cuts at: 33 3132 3817 4810 5001 33
Size: 3099 685 993 191 1121
Fragments arranged by size:
3099 1121 993 685 191
SfoI GGC'GCC
Cuts at: 480 3521 3769 480
Size: 3041 248 2800
Fragments arranged by size:
3041 2800 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6089
SmaI CCC'GGG
Cuts at: 3111 3111
Size: 6089
SmlI C'TyrA_G
Cuts at: 791 1849 2479 2521 3244 4159 4651 4913 791
Size: 1058 630 42 723 915 492 262 1967
Fragments arranged by size:
1967 1058 915 723 630 492 262 42
SnaBI TAC'GTA
Cuts at: 781 781
Size: 6089
SpeI A'CTAG_T
Cuts at: 67 1437 67
Size: 1370 4719
SphI G_CATG'C
Cuts at: 3142 3642 3142
Size: 500 5589
SspI AAT'ATT
Cuts at: 585 1491 2911 585
Size: 906 1420 3763
Fragments arranged by size:
3763 1420 906
StyI C'CwwG_G
Cuts at: 161 339 2292 3223 3577 161
Size: 178 1953 931 354 2673
Fragments arranged by size:
2673 1953 931 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3672 3805 4264 4473 3672
Size: 133 459 209 5288
Fragments arranged by size:
5288 459 209 133
TatI w'GTAC_w
Cuts at: 432 729 1403 1626 2457 5604 432
Size: 297 674 223 831 3147 917
Fragments arranged by size:
3147 917 831 674 297 223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5606 5638 5606
Size: 32 6057
Tth111I GACn'n_nGTC
Cuts at: 3886 3886
Size: 6089
XbaI T'CTAG_A
Cuts at: 1465 1872 3120 1465
Size: 407 1248 4434
Fragments arranged by size:
4434 1248 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 3584 465
Size: 3119 2970
XmaI C'CCGG_G
Cuts at: 3109 3109
Size: 6089
XmnI GAAnn'nnTTC
Cuts at: 118 1868 2426 118
Size: 1750 558 3781
Fragments arranged by size:
3781 1750 558
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BpmI BsaXI
BsiWI BssHII BstEII BstXI BstZ17I ClaI DraIII FseI
FspAI HindIII HpaI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI