Restriction map for
pF1KA2010
(Circular) (Six-base) MAPSORT of: pF1KA2010.seq Check: 2346 from: 1 to: 5573
pF1KA2010 5573 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AatII G_ACGT'C
Cuts at: 767 767
Size: 5573
AccI GT'mk_AC
Cuts at: 2611 2611
Size: 5573
Acc65I G'GTAC_C
Cuts at: 2589 2589
Size: 5573
AclI AA'CG_TT
Cuts at: 5413 5413
Size: 5573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 347 479 767 3398 3830 4577 4845 232
Size: 115 132 288 2631 432 747 268 960
Fragments arranged by size:
2631 960 747 432 288 268 132 115
AflIII A'CryG_T
Cuts at: 959 2925 4029 5006 959
Size: 1966 1104 977 1526
Fragments arranged by size:
1966 1526 1104 977
AgeI A'CCGG_T
Cuts at: 3961 3961
Size: 5573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 5573
AlwNI CAG_nnn'CTG
Cuts at: 871 1387 2878 4445 871
Size: 516 1491 1567 1999
Fragments arranged by size:
1999 1567 1491 516
ApaLI G'TGCA_C
Cuts at: 4343 4343
Size: 5573
ApoI r'AATT_y
Cuts at: 383 820 887 1033 1117 1483 1885 2212 2557
Size: 437 67 146 84 366 402 327 345
Cuts at: 2557 2577 4926 5182 383
Size: 20 2349 256 774
Fragments arranged by size:
2349 774 437 402 366 345 327 256
146 84 67 20
AseI AT'TA_AT
Cuts at: 20 1664 20
Size: 1644 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5573
AvaI C'yCGr_G
Cuts at: 2593 2593
Size: 5573
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1220 1253 1285 1318 1220
Size: 33 32 33 5475
Fragments arranged by size:
5475 33 33 32
BamHI G'GATC_C
Cuts at: 1840 2199 2386 2413 2598 1840
Size: 359 187 27 185 4815
Fragments arranged by size:
4815 359 187 185 27
BanI G'GyrC_C
Cuts at: 175 2589 3003 3251 3286 175
Size: 2414 414 248 35 2462
Fragments arranged by size:
2462 2414 414 248 35
BanII G_rGCy'C
Cuts at: 2293 2587 3617 2293
Size: 294 1030 4249
Fragments arranged by size:
4249 1030 294
BbeI G_GCGC'C
Cuts at: 3007 3255 3007
Size: 248 5325
BbsI GAAGACnn'nnnn_
Cuts at: 1559 2332 1559
Size: 773 4800
BciVI GTATCCnnnnn_n'
Cuts at: 3471 4232 5573 3471
Size: 761 1341 3471
Fragments arranged by size:
3471 1341 761
BclI T'GATC_A
Cuts at: 3093 3093
Size: 5573
BfrBI ATG'CAT
Cuts at: 1515 1515
Size: 5573
BglII A'GATC_T
Cuts at: 3088 5125 3088
Size: 2037 3536
BlpI GC'TnA_GC
Cuts at: 2685 2685
Size: 5573
Bme1580I G_kGCm'C
Cuts at: 178 1734 3198 3291 4347 178
Size: 1556 1464 93 1056 1404
Fragments arranged by size:
1556 1464 1404 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2785 2866 3201 5345 2785
Size: 81 335 2144 3013
Fragments arranged by size:
3013 2144 335 81
BmtI G_CTAG'C
Cuts at: 1456 1456
Size: 5573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 5573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 2384 649
Size: 1735 3838
Bpu10I CC'TnA_GC
Cuts at: 352 872 352
Size: 520 5053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2749 3628 4120 4418 2749
Size: 879 492 298 3904
Fragments arranged by size:
3904 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5277 5277
Size: 5573
BsaAI yAC'GTr
Cuts at: 201 2926 201
Size: 2725 2848
BsaBI GATnn'nnATC
Cuts at: 6 1382 3087 5124 6
Size: 1376 1705 2037 455
Fragments arranged by size:
2037 1705 1376 455
BsaHI Gr'CG_yC
Cuts at: 764 3004 3252 3954 5450 764
Size: 2240 248 702 1496 887
Fragments arranged by size:
2240 1496 887 702 248
BsaWI w'CCGG_w
Cuts at: 1679 2762 3283 3961 4235 4382 4793 1679
Size: 1083 521 678 274 147 411 2459
Fragments arranged by size:
2459 1083 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1388 1418 2138 2168 2453 2483 1388
Size: 30 720 30 285 30 4478
Fragments arranged by size:
4478 720 285 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 1412 478
Size: 934 4639
BseYI C'CCAG_C
Cuts at: 2998 3756 4333 4829 2998
Size: 758 577 496 3742
Fragments arranged by size:
3742 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 5573
BsiEI CG_ry'CG
Cuts at: 104 171 3115 3161 4369 104
Size: 67 2944 46 1208 1308
Fragments arranged by size:
2944 1308 1208 67 46
BsiHKAI G_wGCw'C
Cuts at: 1634 2587 3365 3555 4347 1634
Size: 953 778 190 792 2860
Fragments arranged by size:
2860 953 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 178 1634 1734 2293 2587 3198 3291 3365 3555
Size: 1456 100 559 294 611 93 74 190
Cuts at: 3555 3617 4347 178
Size: 62 730 1404
Fragments arranged by size:
1456 1404 730 611 559 294 190 100
93 74 62
BspEI T'CCGG_A
Cuts at: 1679 4793 1679
Size: 3114 2459
BspHI T'CATG_A
Cuts at: 4749 4749
Size: 5573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2623 2837 3139 3520 2623
Size: 214 302 381 4676
Fragments arranged by size:
4676 381 302 214
BsrBI CCG'CTC
Cuts at: 50 207 3865 3919 5403 5568 50
Size: 157 3658 54 1484 165 55
Fragments arranged by size:
3658 1484 165 157 55 54
BsrDI GCAATG_nn'
Cuts at: 3485 3485
Size: 5573
BsrFI r'CCGG_y
Cuts at: 155 3571 3752 3961 155
Size: 3416 181 209 1767
Fragments arranged by size:
3416 1767 209 181
BsrGI T'GTAC_A
Cuts at: 137 1968 137
Size: 1831 3742
BssSI C'ACGA_G
Cuts at: 2467 3844 4202 2467
Size: 1377 358 3838
Fragments arranged by size:
3838 1377 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1512 1729 2950 1512
Size: 217 1221 4135
Fragments arranged by size:
4135 1221 217
BstBI TT'CG_AA
Cuts at: 1425 3934 1425
Size: 2509 3064
BstYI r'GATC_y
Cuts at: 1 867 1383 1840 2199 2386 2395 2413 2598
Size: 866 516 457 359 187 9 18 185
Cuts at: 2598 3065 3088 3423 3669 4681 4767 4779 5125
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5125 1
Size: 449
Fragments arranged by size:
1012 866 516 467 457 449 359 346
335 246 187 185 86 23 18 12
9
BtgI C'CryG_G
Cuts at: 425 1289 425
Size: 864 4709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 470 3523 3704 3867 470
Size: 3053 181 163 2176
Fragments arranged by size:
3053 2176 181 163
BtsI GCAGTG_nn'
Cuts at: 166 284 1457 1523 2816 166
Size: 118 1173 66 1293 2923
Fragments arranged by size:
2923 1293 1173 118 66
DraI TTT'AAA
Cuts at: 846 1112 1743 1980 2572 846
Size: 266 631 237 592 3847
Fragments arranged by size:
3847 631 592 266 237
DrdI GACnn_nn'nnGTC
Cuts at: 3279 4137 3279
Size: 858 4715
EaeI y'GGCC_r
Cuts at: 3158 3332 3723 3750 5095 3158
Size: 174 391 27 1345 3636
Fragments arranged by size:
3636 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3158 3158
Size: 5573
EarI CTCTTCn'nnn_
Cuts at: 455 575 2070 2099 3596 3806 455
Size: 120 1495 29 1497 210 2222
Fragments arranged by size:
2222 1497 1495 210 120 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3781 4091 4237 5383 3781
Size: 310 146 1146 3971
Fragments arranged by size:
3971 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2585 2585
Size: 5573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 347 479 649 767 2384 3398 3830 4577
Size: 115 132 170 118 1617 1014 432 747
Cuts at: 4577 4845 232
Size: 268 960
Fragments arranged by size:
1617 1014 960 747 432 268 170 132
118 115
EcoO109I rG'GnC_Cy
Cuts at: 417 441 2712 417
Size: 24 2271 3278
Fragments arranged by size:
3278 2271 24
EcoRI G'AATT_C
Cuts at: 2577 2577
Size: 5573
EcoRV GAT'ATC
Cuts at: 1674 2071 1674
Size: 397 5176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1231 3592 1231
Size: 2361 3212
FspI TGC'GCA
Cuts at: 3354 3354
Size: 5573
HaeII r_GCGC'y
Cuts at: 3007 3255 4277 5256 3007
Size: 248 1022 979 3324
Fragments arranged by size:
3324 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 469 498 501 1246 1278 1289 1321 1388
Size: 3 29 3 745 32 11 32 67
Cuts at: 1388 1420 1894 1926 3415 3445 3447 3477 466
Size: 32 474 32 1489 30 2 30 2562
Fragments arranged by size:
2562 1489 745 474 67 32 32 32
32 30 30 29 11 3 3 2
HincII GTy'rAC
Cuts at: 1104 1437 1616 2612 1104
Size: 333 179 996 4065
Fragments arranged by size:
4065 996 333 179
HindIII A'AGCT_T
Cuts at: 2302 2302
Size: 5573
HpaI GTT'AAC
Cuts at: 1104 1616 1104
Size: 512 5061
KasI G'GCGC_C
Cuts at: 3003 3251 3003
Size: 248 5325
KpnI G_GTAC'C
Cuts at: 2593 2593
Size: 5573
MluI A'CGCG_T
Cuts at: 5006 5006
Size: 5573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4244 4428 4244
Size: 184 5389
MscI TGG'CCA
Cuts at: 3334 3334
Size: 5573
MslI CAynn'nnrTG
Cuts at: 644 683 3562 3689 644
Size: 39 2879 127 2528
Fragments arranged by size:
2879 2528 127 39
MspA1I CmG'CkG
Cuts at: 2684 2998 3358 4371 4616 2684
Size: 314 360 1013 245 3641
Fragments arranged by size:
3641 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3754 3754
Size: 5573
NarI GG'CG_CC
Cuts at: 3004 3252 3004
Size: 248 5325
NcoI C'CATG_G
Cuts at: 1289 1289
Size: 5573
NdeI CA'TA_TG
Cuts at: 1367 1367
Size: 5573
NgoMIV G'CCGG_C
Cuts at: 3752 3752
Size: 5573
NheI G'CTAG_C
Cuts at: 1452 1452
Size: 5573
NsiI A_TGCA'T
Cuts at: 1517 1517
Size: 5573
NspI r_CATG'y
Cuts at: 187 222 963 1017 1452 1519 1714 2626 3126
Size: 35 741 54 435 67 195 912 500
Cuts at: 3126 4033 187
Size: 907 1727
Fragments arranged by size:
1727 912 907 741 500 435 195 67
54 35
PciI A'CATG_T
Cuts at: 959 4029 959
Size: 3070 2503
PflMI CCAn_nnn'nTGG
Cuts at: 1413 1593 1413
Size: 180 5393
PfoI T'CCnGG_A
Cuts at: 626 2373 2409 626
Size: 1747 36 3790
Fragments arranged by size:
3790 1747 36
PmeI GTTT'AAAC
Cuts at: 2572 2572
Size: 5573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 266 1676 1708 4712 4744 234
Size: 32 1410 32 3004 32 1063
Fragments arranged by size:
3004 1410 1063 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 417 441 417
Size: 24 5549
PstI C_TGCA'G
Cuts at: 2620 3305 2620
Size: 685 4888
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5573
PvuII CAG'CTG
Cuts at: 2998 3358 2998
Size: 360 5213
RsrII CG'GwC_CG
Cuts at: 3768 3768
Size: 5573
SacI G_AGCT'C
Cuts at: 2587 2587
Size: 5573
SalI G'TCGA_C
Cuts at: 2610 2610
Size: 5573
SapI GCTCTTCn'nnn_
Cuts at: 3596 3806 3596
Size: 210 5363
SbfI CC_TGCA'GG
Cuts at: 2620 2620
Size: 5573
ScaI AGT'ACT
Cuts at: 5090 5090
Size: 5573
SfcI C'TryA_G
Cuts at: 33 1141 2153 2616 3301 4294 4485 33
Size: 1108 1012 463 685 993 191 1121
Fragments arranged by size:
1121 1108 1012 993 685 463 191
SfoI GGC'GCC
Cuts at: 3005 3253 3005
Size: 248 5325
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5573
SmaI CCC'GGG
Cuts at: 2595 2595
Size: 5573
SmlI C'TyrA_G
Cuts at: 2728 3643 4135 4397 2728
Size: 915 492 262 3904
Fragments arranged by size:
3904 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5573
SphI G_CATG'C
Cuts at: 1714 2626 3126 1714
Size: 912 500 4161
Fragments arranged by size:
4161 912 500
SspI AAT'ATT
Cuts at: 663 663
Size: 5573
StuI AGG'CCT
Cuts at: 2465 2465
Size: 5573
StyI C'CwwG_G
Cuts at: 1215 1289 2707 3061 1215
Size: 74 1418 354 3727
Fragments arranged by size:
3727 1418 354 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 933 3156 3289 3748 3957 127
Size: 806 2223 133 459 209 1743
Fragments arranged by size:
2223 1743 806 459 209 133
TatI w'GTAC_w
Cuts at: 137 1968 5088 137
Size: 1831 3120 622
Fragments arranged by size:
3120 1831 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 2453 2485 5090 5122 145
Size: 32 2276 32 2605 32 596
Fragments arranged by size:
2605 2276 596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1176 3370 1176
Size: 2194 3379
XbaI T'CTAG_A
Cuts at: 2604 2604
Size: 5573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3068 3068
Size: 5573
XmaI C'CCGG_G
Cuts at: 2593 2593
Size: 5573
XmnI GAAnn'nnTTC
Cuts at: 854 1034 2258 854
Size: 180 1224 4169
Fragments arranged by size:
4169 1224 180
ZraI GAC'GTC
Cuts at: 765 765
Size: 5573
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BglI BmgBI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI MfeI NotI NruI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI