Restriction map for
pF1KA1995
(Circular) (Six-base) MAPSORT of: pF1KA1995.seq Check: 7356 from: 1 to: 6050
pF1KA1995 6050 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2784 2913 2784
Size: 129 5921
AccI GT'mk_AC
Cuts at: 3033 3088 3033
Size: 55 5995
Acc65I G'GTAC_C
Cuts at: 572 3066 572
Size: 2494 3556
AclI AA'CG_TT
Cuts at: 5890 5890
Size: 6050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1526 2693 3875 4307 5054 5322 1133
Size: 393 1167 1182 432 747 268 1861
Fragments arranged by size:
1861 1182 1167 747 432 393 268
AflIII A'CryG_T
Cuts at: 758 3402 4506 5483 758
Size: 2644 1104 977 1325
Fragments arranged by size:
2644 1325 1104 977
AgeI A'CCGG_T
Cuts at: 4438 4438
Size: 6050
AleI CACnn'nnGTG
Cuts at: 1285 1285
Size: 6050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 618 837 1247 2806 618
Size: 219 410 1559 3862
Fragments arranged by size:
3862 1559 410 219
AlwNI CAG_nnn'CTG
Cuts at: 1191 2528 2620 2820 3355 4922 1191
Size: 1337 92 200 535 1567 2319
Fragments arranged by size:
2319 1567 1337 535 200 92
ApaLI G'TGCA_C
Cuts at: 4820 4820
Size: 6050
ApoI r'AATT_y
Cuts at: 905 1786 2292 2566 3054 5403 5659 905
Size: 881 506 274 488 2349 256 1296
Fragments arranged by size:
2349 1296 881 506 488 274 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
AvaI C'yCGr_G
Cuts at: 189 2358 3070 189
Size: 2169 712 3169
Fragments arranged by size:
3169 2169 712
BamHI G'GATC_C
Cuts at: 981 2976 3075 981
Size: 1995 99 3956
Fragments arranged by size:
3956 1995 99
BanI G'GyrC_C
Cuts at: 289 572 3008 3066 3480 3728 3763 289
Size: 283 2436 58 414 248 35 2576
Fragments arranged by size:
2576 2436 414 283 248 58 35
BanII G_rGCy'C
Cuts at: 773 2353 3064 4094 773
Size: 1580 711 1030 2729
Fragments arranged by size:
2729 1580 1030 711
BbeI G_GCGC'C
Cuts at: 293 3484 3732 293
Size: 3191 248 2611
Fragments arranged by size:
3191 2611 248
BbvCI CC'TCA_GC
Cuts at: 1540 1540
Size: 6050
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 889 923 889
Size: 34 6016
BciVI GTATCCnnnnn_n'
Cuts at: 3948 4709 6050 3948
Size: 761 1341 3948
Fragments arranged by size:
3948 1341 761
BclI T'GATC_A
Cuts at: 2063 3570 2063
Size: 1507 4543
BfrBI ATG'CAT
Cuts at: 2934 2934
Size: 6050
BglII A'GATC_T
Cuts at: 3565 5602 3565
Size: 2037 4013
BlpI GC'TnA_GC
Cuts at: 3162 3162
Size: 6050
Bme1580I G_kGCm'C
Cuts at: 1221 3675 3768 4824 1221
Size: 2454 93 1056 2447
Fragments arranged by size:
2454 2447 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1651 1746 3262 3343 3678 5822 1651
Size: 95 1516 81 335 2144 1879
Fragments arranged by size:
2144 1879 1516 335 95 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 348 1578 4417 348
Size: 1230 2839 1981
Fragments arranged by size:
2839 1981 1230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 2854 749
Size: 2105 3945
Bpu10I CC'TnA_GC
Cuts at: 986 1540 986
Size: 554 5496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3226 4105 4597 4895 3226
Size: 879 492 298 4381
Fragments arranged by size:
4381 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 987 1083 1566 2076 2166 5754 987
Size: 96 483 510 90 3588 1283
Fragments arranged by size:
3588 1283 510 483 96 90
BsaAI yAC'GTr
Cuts at: 3403 3403
Size: 6050
BsaBI GATnn'nnATC
Cuts at: 6 2111 3564 5601 6
Size: 2105 1453 2037 455
Fragments arranged by size:
2105 2037 1453 455
BsaHI Gr'CG_yC
Cuts at: 290 3481 3729 4431 5927 290
Size: 3191 248 702 1496 413
Fragments arranged by size:
3191 1496 702 413 248
BsaWI w'CCGG_w
Cuts at: 3239 3760 4438 4712 4859 5270 3239
Size: 521 678 274 147 411 4019
Fragments arranged by size:
4019 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1327 1357 1327
Size: 30 6020
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2378 2585 2756 2378
Size: 207 171 5672
Fragments arranged by size:
5672 207 171
BseYI C'CCAG_C
Cuts at: 117 1323 1797 1992 2743 3037 3475 4233 4810
Size: 1206 474 195 751 294 438 758 577
Cuts at: 4810 5306 117
Size: 496 861
Fragments arranged by size:
1206 861 758 751 577 496 474 438
294 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1253 2624 2802 241
Size: 1012 1371 178 3489
Fragments arranged by size:
3489 1371 1012 178
BsiEI CG_ry'CG
Cuts at: 104 3592 3638 4846 104
Size: 3488 46 1208 1308
Fragments arranged by size:
3488 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 773 2353 3064 3842 4032 4824 773
Size: 1580 711 778 190 792 1999
Fragments arranged by size:
1999 1580 792 778 711 190
BsmI GAATG_Cn'
Cuts at: 2934 2934
Size: 6050
BsmBI CGTCTCn'nnnn_
Cuts at: 727 727
Size: 6050
Bsp1286I G_dGCh'C
Cuts at: 773 1221 2353 3064 3675 3768 3842 4032 4094
Size: 448 1132 711 611 93 74 190 62
Cuts at: 4094 4824 773
Size: 730 1999
Fragments arranged by size:
1999 1132 730 711 611 448 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5270 5270
Size: 6050
BspHI T'CATG_A
Cuts at: 5226 5226
Size: 6050
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1215 1548 2784 2913 3100 3314 3616 3997 1215
Size: 333 1236 129 187 214 302 381 3268
Fragments arranged by size:
3268 1236 381 333 302 214 187 129
BsrBI CCG'CTC
Cuts at: 50 4342 4396 5880 6045 50
Size: 4292 54 1484 165 55
Fragments arranged by size:
4292 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1121 1910 2093 2139 2162 3962 1121
Size: 789 183 46 23 1800 3209
Fragments arranged by size:
3209 1800 789 183 46 23
BsrFI r'CCGG_y
Cuts at: 226 1955 4048 4229 4438 226
Size: 1729 2093 181 209 1838
Fragments arranged by size:
2093 1838 1729 209 181
BsrGI T'GTAC_A
Cuts at: 1277 1277
Size: 6050
BssSI C'ACGA_G
Cuts at: 4321 4679 4321
Size: 358 5692
BstAPI GCAn_nnn'nTGC
Cuts at: 1123 3427 1123
Size: 2304 3746
BstBI TT'CG_AA
Cuts at: 2544 4411 2544
Size: 1867 4183
BstXI CCAn_nnnn'nTGG
Cuts at: 616 616
Size: 6050
BstYI r'GATC_y
Cuts at: 1 981 1901 2221 2976 3075 3542 3565 3900
Size: 980 920 320 755 99 467 23 335
Cuts at: 3900 4146 5158 5244 5256 5602 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 980 920 755 467 449 346 335
320 246 99 86 23 12
BtgI C'CryG_G
Cuts at: 285 725 914 1314 1329 2253 2498 285
Size: 440 189 400 15 924 245 3837
Fragments arranged by size:
3837 924 440 400 245 189 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4000 4181 4344 4000
Size: 181 163 5706
Fragments arranged by size:
5706 181 163
BtsI GCAGTG_nn'
Cuts at: 135 418 602 2325 3293 135
Size: 283 184 1723 968 2892
Fragments arranged by size:
2892 1723 968 283 184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1346 1381 1965 2000 2301 2336 1346
Size: 35 584 35 301 35 5060
Fragments arranged by size:
5060 584 301 35 35 35
DraI TTT'AAA
Cuts at: 3049 3049
Size: 6050
DrdI GACnn_nn'nnGTC
Cuts at: 369 3756 4614 369
Size: 3387 858 1805
Fragments arranged by size:
3387 1805 858
EaeI y'GGCC_r
Cuts at: 1877 2145 3635 3809 4200 4227 5572 1877
Size: 268 1490 174 391 27 1345 2355
Fragments arranged by size:
2355 1490 1345 391 268 174 27
EagI C'GGCC_G
Cuts at: 3635 3635
Size: 6050
EarI CTCTTCn'nnn_
Cuts at: 551 1031 1652 2387 2639 2957 4073 4283 551
Size: 480 621 735 252 318 1116 210 2318
Fragments arranged by size:
2318 1116 735 621 480 318 252 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 256 4258 4568 4714 5860 256
Size: 4002 310 146 1146 446
Fragments arranged by size:
4002 1146 446 310 146
EcoICRI GAG'CTC
Cuts at: 771 2351 3062 771
Size: 1580 711 3759
Fragments arranged by size:
3759 1580 711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 1133 1526 2693 2854 3875 4307 5054 5322
Size: 384 393 1167 161 1021 432 747 268
Cuts at: 5322 749
Size: 1477
Fragments arranged by size:
1477 1167 1021 747 432 393 384 268
161
EcoNI CCTnn'n_nnAGG
Cuts at: 849 976 849
Size: 127 5923
EcoO109I rG'GnC_Cy
Cuts at: 199 2041 3189 199
Size: 1842 1148 3060
Fragments arranged by size:
3060 1842 1148
EcoRI G'AATT_C
Cuts at: 905 1786 2292 3054 905
Size: 881 506 762 3901
Fragments arranged by size:
3901 881 762 506
EcoRV GAT'ATC
Cuts at: 807 2190 807
Size: 1383 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3006 4069 3006
Size: 1063 4987
FspI TGC'GCA
Cuts at: 3831 3831
Size: 6050
HaeII r_GCGC'y
Cuts at: 209 293 3484 3732 4754 5733 209
Size: 84 3191 248 1022 979 526
Fragments arranged by size:
3191 1022 979 526 248 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1327 1359 2575 2607 3026 3058 3892 3922 3924
Size: 32 1216 32 419 32 834 30 2
Cuts at: 3924 3954 1327
Size: 30 3423
Fragments arranged by size:
3423 1216 834 419 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 924 1748 3089 924
Size: 824 1341 3885
Fragments arranged by size:
3885 1341 824
KasI G'GCGC_C
Cuts at: 289 3480 3728 289
Size: 3191 248 2611
Fragments arranged by size:
3191 2611 248
KpnI G_GTAC'C
Cuts at: 576 3070 576
Size: 2494 3556
MluI A'CGCG_T
Cuts at: 5483 5483
Size: 6050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4721 4905 4721
Size: 184 5866
MscI TGG'CCA
Cuts at: 1879 3811 1879
Size: 1932 4118
MslI CAynn'nnrTG
Cuts at: 112 1285 1483 4039 4166 112
Size: 1173 198 2556 127 1996
Fragments arranged by size:
2556 1996 1173 198 127
MspA1I CmG'CkG
Cuts at: 287 1323 1527 1936 1992 3161 3475 3835 4848
Size: 1036 204 409 56 1169 314 360 1013
Cuts at: 4848 5093 287
Size: 245 1244
Fragments arranged by size:
1244 1169 1036 1013 409 360 314 245
204 56
NaeI GCC'GGC
Cuts at: 228 1957 4231 228
Size: 1729 2274 2047
Fragments arranged by size:
2274 2047 1729
NarI GG'CG_CC
Cuts at: 290 3481 3729 290
Size: 3191 248 2611
Fragments arranged by size:
3191 2611 248
NcoI C'CATG_G
Cuts at: 725 914 1314 1329 2498 725
Size: 189 400 15 1169 4277
Fragments arranged by size:
4277 1169 400 189 15
NgoMIV G'CCGG_C
Cuts at: 226 1955 4229 226
Size: 1729 2274 2047
Fragments arranged by size:
2274 2047 1729
NsiI A_TGCA'T
Cuts at: 2936 2936
Size: 6050
NspI r_CATG'y
Cuts at: 762 1296 1366 1818 3103 3603 4510 762
Size: 534 70 452 1285 500 907 2302
Fragments arranged by size:
2302 1285 907 534 500 452 70
PciI A'CATG_T
Cuts at: 758 4506 758
Size: 3748 2302
PflMI CCAn_nnn'nTGG
Cuts at: 1191 1191
Size: 6050
PfoI T'CCnGG_A
Cuts at: 2237 2237
Size: 6050
PmeI GTTT'AAAC
Cuts at: 3049 3049
Size: 6050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 5189
Size: 32 6018
PpuMI rG'GwC_Cy
Cuts at: 2041 2041
Size: 6050
PshAI GACnn'nnGTC
Cuts at: 191 1095 191
Size: 904 5146
PsiI TTA'TAA
Cuts at: 1898 1898
Size: 6050
PspXI vC'TCGA_Gb
Cuts at: 189 189
Size: 6050
PstI C_TGCA'G
Cuts at: 428 1005 2733 2795 2891 3097 3782 428
Size: 577 1728 62 96 206 685 2696
Fragments arranged by size:
2696 1728 685 577 206 96 62
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6050
PvuII CAG'CTG
Cuts at: 1323 1527 1936 1992 3475 3835 1323
Size: 204 409 56 1483 360 3538
Fragments arranged by size:
3538 1483 409 360 204 56
RsrII CG'GwC_CG
Cuts at: 4245 4245
Size: 6050
SacI G_AGCT'C
Cuts at: 773 2353 3064 773
Size: 1580 711 3759
Fragments arranged by size:
3759 1580 711
SacII CC_GC'GG
Cuts at: 288 288
Size: 6050
SalI G'TCGA_C
Cuts at: 3087 3087
Size: 6050
SanDI GG'GwC_CC
Cuts at: 2041 2041
Size: 6050
SapI GCTCTTCn'nnn_
Cuts at: 2639 4073 4283 2639
Size: 1434 210 4406
Fragments arranged by size:
4406 1434 210
SbfI CC_TGCA'GG
Cuts at: 3097 3097
Size: 6050
ScaI AGT'ACT
Cuts at: 937 5567 937
Size: 4630 1420
SfcI C'TryA_G
Cuts at: 33 424 1001 1127 1212 2729 2791 2854 2887
Size: 391 577 126 85 1517 62 63 33
Cuts at: 2887 3030 3093 3778 4771 4962 33
Size: 143 63 685 993 191 1121
Fragments arranged by size:
1517 1121 993 685 577 391 191 143
126 85 63 63 62 33
SfoI GGC'GCC
Cuts at: 291 3482 3730 291
Size: 3191 248 2611
Fragments arranged by size:
3191 2611 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6050
SmaI CCC'GGG
Cuts at: 2360 3072 2360
Size: 712 5338
SmlI C'TyrA_G
Cuts at: 189 3205 4120 4612 4874 189
Size: 3016 915 492 262 1365
Fragments arranged by size:
3016 1365 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6050
SphI G_CATG'C
Cuts at: 3103 3603 3103
Size: 500 5550
SspI AAT'ATT
Cuts at: 496 651 496
Size: 155 5895
StuI AGG'CCT
Cuts at: 1699 1699
Size: 6050
StyI C'CwwG_G
Cuts at: 662 725 914 1093 1314 1329 1694 1874 2046
Size: 63 189 179 221 15 365 180 172
Cuts at: 2046 2498 3184 3538 662
Size: 452 686 354 3174
Fragments arranged by size:
3174 686 452 365 354 221 189 180
179 172 63 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1134 1275 2477 3633 3766 4225 4434 1134
Size: 141 1202 1156 133 459 209 2750
Fragments arranged by size:
2750 1202 1156 459 209 141 133
TatI w'GTAC_w
Cuts at: 788 935 1277 5565 788
Size: 147 342 4288 1273
Fragments arranged by size:
4288 1273 342 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 2648 2680 5567 5599 127
Size: 32 2489 32 2887 32 578
Fragments arranged by size:
2887 2489 578 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1752 3847 1752
Size: 2095 3955
XbaI T'CTAG_A
Cuts at: 1014 3081 1014
Size: 2067 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 276 2496 3545 114
Size: 162 2220 1049 2619
Fragments arranged by size:
2619 2220 1049 162
XhoI C'TCGA_G
Cuts at: 189 189
Size: 6050
XmaI C'CCGG_G
Cuts at: 2358 3070 2358
Size: 712 5338
XmnI GAAnn'nnTTC
Cuts at: 1534 1534
Size: 6050
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BglI BmgBI BmtI BsiWI BssHII BstEII
BstZ17I Bsu36I ClaI DraIII FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PspOMI PsrI SexAI SfiI SgrAI SnaBI SrfI SwaI
ZraI