Restriction map for
pF1KA1983
(Circular) (Six-base) MAPSORT of: pF1KA1983.seq Check: 2215 from: 1 to: 4331
pF1KA1983 4331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 307 772 1181 2156 2588 2941 3603 278
Size: 29 465 409 975 432 353 662 1006
Fragments arranged by size:
1006 975 662 465 432 409 353 29
AflIII A'CryG_T
Cuts at: 200 625 1683 3483 3764 200
Size: 425 1058 1800 281 767
Fragments arranged by size:
1800 1058 767 425 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 779 779
Size: 4331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 1159
Size: 32 4299
AlwNI CAG_nnn'CTG
Cuts at: 1636 3074 1636
Size: 1438 2893
ApaI G_GGCC'C
Cuts at: 877 1018 1082 877
Size: 141 64 4126
Fragments arranged by size:
4126 141 64
ApaLI G'TGCA_C
Cuts at: 411 3169 411
Size: 2758 1573
ApoI r'AATT_y
Cuts at: 343 1225 1335 3684 3940 343
Size: 882 110 2349 256 734
Fragments arranged by size:
2349 882 734 256 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 1351 1351
Size: 4331
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 111 1035 1181 1347 1761 2009 2044 111
Size: 924 146 166 414 248 35 2398
Fragments arranged by size:
2398 924 414 248 166 146 35
BanII G_rGCy'C
Cuts at: 305 877 958 1018 1082 1261 1308 1345 2375
Size: 572 81 60 64 179 47 37 1030
Cuts at: 2375 305
Size: 2261
Fragments arranged by size:
2261 1030 572 179 81 64 60 47
37
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 284 628 284
Size: 344 3987
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 367 401 367
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 448 2229 3285 4331 448
Size: 1781 1056 1046 448
Fragments arranged by size:
1781 1056 1046 448
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BfrBI ATG'CAT
Cuts at: 362 362
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 155 596 881 953 1013 155
Size: 441 285 72 60 3473
Fragments arranged by size:
3473 441 285 72 60
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 415 549 877 1018 1038 1082 1184 1956 2049
Size: 134 328 141 20 44 102 772 93
Cuts at: 2049 3173 415
Size: 1124 1573
Fragments arranged by size:
1573 1124 772 328 141 134 102 93
44 20
BmrI ACTGGGnnnn_n'
Cuts at: 1318 1543 1624 1959 4103 1318
Size: 225 81 335 2144 1546
Fragments arranged by size:
2144 1546 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 1283 1076
Size: 207 4124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1320 1507 2386 3100 3398 1320
Size: 187 879 714 298 2253
Fragments arranged by size:
2253 879 714 298 187
BsaI GGTCTCn'nnnn_
Cuts at: 1094 4035 1094
Size: 2941 1390
BsaAI yAC'GTr
Cuts at: 203 1684 203
Size: 1481 2850
BsaBI GATnn'nnATC
Cuts at: 6 1271 1845 3882 6
Size: 1265 574 2037 455
Fragments arranged by size:
2037 1265 574 455
BsaHI Gr'CG_yC
Cuts at: 1762 2010 2712 4208 1762
Size: 248 702 1496 1885
Fragments arranged by size:
1885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 479 1184 1520 2041 2719 3130 3277 3551 479
Size: 705 336 521 678 411 147 274 1259
Fragments arranged by size:
1259 705 678 521 411 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 153 183 153
Size: 30 4301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 149 232 1082 1219 149
Size: 83 850 137 3261
Fragments arranged by size:
3261 850 137 83
BseYI C'CCAG_C
Cuts at: 153 1231 1756 2514 3179 3587 153
Size: 1078 525 758 665 408 897
Fragments arranged by size:
1078 897 758 665 525 408
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3149 104
Size: 1769 46 1230 1286
Fragments arranged by size:
1769 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 305 415 1345 2123 2313 3173 305
Size: 110 930 778 190 860 1463
Fragments arranged by size:
1463 930 860 778 190 110
Bsp1286I G_dGCh'C
Cuts at: 305 415 549 877 958 1018 1038 1082 1184
Size: 110 134 328 81 60 20 44 102
Cuts at: 1184 1261 1308 1345 1956 2049 2123 2313 2375
Size: 77 47 37 611 93 74 190 62
Cuts at: 2375 3173 305
Size: 798 1463
Fragments arranged by size:
1463 798 611 328 190 134 110 102
93 81 77 74 62 60 47 44
37 20
BspHI T'CATG_A
Cuts at: 2763 2763
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 1595 1897 2278 1381
Size: 214 302 381 3434
Fragments arranged by size:
3434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 537 1152 2243 537
Size: 615 1091 2625
Fragments arranged by size:
2625 1091 615
BsrFI r'CCGG_y
Cuts at: 2329 2510 2329
Size: 181 4150
BssSI C'ACGA_G
Cuts at: 2602 3310 2602
Size: 708 3623
BstAPI GCAn_nnn'nTGC
Cuts at: 741 1708 741
Size: 967 3364
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 1254 1254
Size: 4331
BstYI r'GATC_y
Cuts at: 1 1356 1823 1846 2181 2427 2733 2745 2831
Size: 1355 467 23 335 246 306 12 86
Cuts at: 2831 3883 1
Size: 1052 449
Fragments arranged by size:
1355 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1220 1220
Size: 4331
BtgI C'CryG_G
Cuts at: 107 949 968 1247 107
Size: 842 19 279 3191
Fragments arranged by size:
3191 842 279 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 415 1574 415
Size: 1159 3172
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 3381 2037
Size: 1344 2987
EaeI y'GGCC_r
Cuts at: 426 660 1916 2090 2481 2508 3853 426
Size: 234 1256 174 391 27 1345 904
Fragments arranged by size:
1345 1256 904 391 234 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 1038 1200 2354 2564 1038
Size: 162 1154 210 2805
Fragments arranged by size:
2805 1154 210 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 3281 3427 4141 2539
Size: 742 146 714 2729
Fragments arranged by size:
2729 742 714 146
EcoICRI GAG'CTC
Cuts at: 303 1343 303
Size: 1040 3291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 307 772 1076 1181 1283 2156 2588 2941
Size: 29 465 304 105 102 873 432 353
Cuts at: 2941 3603 278
Size: 662 1006
Fragments arranged by size:
1006 873 662 465 432 353 304 105
102 29
EcoO109I rG'GnC_Cy
Cuts at: 847 873 874 928 964 1014 1015 1096 1470
Size: 26 1 54 36 50 1 81 374
Cuts at: 1470 847
Size: 3708
Fragments arranged by size:
3708 374 81 54 50 36 26 1
1
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1075 1765 2013 3243 4014 1075
Size: 690 248 1230 771 1392
Fragments arranged by size:
1392 1230 771 690 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 347 379 1243 1275 2173 2203 2205
Size: 32 162 32 864 32 898 30 2
Cuts at: 2205 2235 153
Size: 30 2249
Fragments arranged by size:
2249 898 864 162 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3090 3274 3090
Size: 184 4147
MscI TGG'CCA
Cuts at: 662 2092 662
Size: 1430 2901
MslI CAynn'nnrTG
Cuts at: 2320 2447 2320
Size: 127 4204
MspA1I CmG'CkG
Cuts at: 153 174 581 750 787 1442 1756 2116 2900
Size: 21 407 169 37 655 314 360 784
Cuts at: 2900 3145 153
Size: 245 1339
Fragments arranged by size:
1339 784 655 407 360 314 245 169
37 21
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 968 1247 107
Size: 861 279 3191
Fragments arranged by size:
3191 861 279
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NruI TCG'CGA
Cuts at: 265 265
Size: 4331
NsiI A_TGCA'T
Cuts at: 364 364
Size: 4331
NspI r_CATG'y
Cuts at: 314 629 713 1384 1884 3487 314
Size: 315 84 671 500 1603 1158
Fragments arranged by size:
1603 1158 671 500 315 84
PciI A'CATG_T
Cuts at: 625 3483 625
Size: 2858 1473
PfoI T'CCnGG_A
Cuts at: 450 605 843 1092 450
Size: 155 238 249 3689
Fragments arranged by size:
3689 249 238 155
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PmlI CAC'GTG
Cuts at: 203 203
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 847 964 1096 847
Size: 117 132 4082
Fragments arranged by size:
4082 132 117
PspOMI G'GGCC_C
Cuts at: 873 1014 1078 873
Size: 141 64 4126
Fragments arranged by size:
4126 141 64
PstI C_TGCA'G
Cuts at: 749 1166 1378 2063 749
Size: 417 212 685 3017
Fragments arranged by size:
3017 685 417 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 153 750 787 1756 2116 153
Size: 597 37 969 360 2368
Fragments arranged by size:
2368 969 597 360 37
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 305 1345 305
Size: 1040 3291
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SanDI GG'GwC_CC
Cuts at: 964 964
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 210 315 745 1162 1374 2059 3027 3218
Size: 177 105 430 417 212 685 968 191
Cuts at: 3218 33
Size: 1146
Fragments arranged by size:
1146 968 685 430 417 212 191 177
105
SfiI GGCCn_nnn'nGGCC
Cuts at: 1013 1013
Size: 4331
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 1299 1486 2401 3115 3377 1299
Size: 187 915 714 262 2253
Fragments arranged by size:
2253 915 714 262 187
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
StyI C'CwwG_G
Cuts at: 107 566 968 1247 1465 1819 107
Size: 459 402 279 218 354 2619
Fragments arranged by size:
2619 459 402 354 279 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1914 2047 2506 2715 1914
Size: 133 459 209 3530
Fragments arranged by size:
3530 459 209 133
TatI w'GTAC_w
Cuts at: 439 3846 439
Size: 3407 924
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 528 560 825 857 3848 3880 528
Size: 32 265 32 2991 32 979
Fragments arranged by size:
2991 979 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1826 150
Size: 1676 2655
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AscI
AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI PacI PasI PflMI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI