Restriction map for
pF1KA1962
(Circular) (Six-base) MAPSORT of: pF1KA1962.seq Check: 2402 from: 1 to: 5345
pF1KA1962 5345 bp
With 182 enzymes: SgfI *
November 10, 2008 09:34 ..
AccI GT'mk_AC
Cuts at: 1076 2383 1076
Size: 1307 4038
Acc65I G'GTAC_C
Cuts at: 2361 2361
Size: 5345
AclI AA'CG_TT
Cuts at: 5185 5185
Size: 5345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1034 1107 1205 1770 2106 3170 3602 4349 4617
Size: 73 98 565 336 1064 432 747 268
Cuts at: 4617 1034
Size: 1762
Fragments arranged by size:
1762 1064 747 565 432 336 268 98
73
AflIII A'CryG_T
Cuts at: 1762 2697 3801 4778 1762
Size: 935 1104 977 2329
Fragments arranged by size:
2329 1104 977 935
AgeI A'CCGG_T
Cuts at: 3733 3733
Size: 5345
AlwNI CAG_nnn'CTG
Cuts at: 348 408 2650 4217 348
Size: 60 2242 1567 1476
Fragments arranged by size:
2242 1567 1476 60
ApaLI G'TGCA_C
Cuts at: 4115 4115
Size: 5345
ApoI r'AATT_y
Cuts at: 727 945 1003 1484 1652 1736 1820 1878 1904
Size: 218 58 481 168 84 84 58 26
Cuts at: 1904 2072 2156 2240 2349 4698 4954 727
Size: 168 84 84 109 2349 256 1118
Fragments arranged by size:
2349 1118 481 256 218 168 168 109
84 84 84 84 58 58 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5345
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
AvaI C'yCGr_G
Cuts at: 2365 2365
Size: 5345
BamHI G'GATC_C
Cuts at: 2370 2370
Size: 5345
BanI G'GyrC_C
Cuts at: 860 2361 2775 3023 3058 860
Size: 1501 414 248 35 3147
Fragments arranged by size:
3147 1501 414 248 35
BanII G_rGCy'C
Cuts at: 335 2359 3389 335
Size: 2024 1030 2291
Fragments arranged by size:
2291 2024 1030
BbeI G_GCGC'C
Cuts at: 2779 3027 2779
Size: 248 5097
BbsI GAAGACnn'nnnn_
Cuts at: 2290 2290
Size: 5345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5311
BciVI GTATCCnnnnn_n'
Cuts at: 3243 4004 5345 3243
Size: 761 1341 3243
Fragments arranged by size:
3243 1341 761
BclI T'GATC_A
Cuts at: 876 2865 876
Size: 1989 3356
BglII A'GATC_T
Cuts at: 2860 4897 2860
Size: 2037 3308
BlpI GC'TnA_GC
Cuts at: 2457 2457
Size: 5345
Bme1580I G_kGCm'C
Cuts at: 125 2970 3063 4119 125
Size: 2845 93 1056 1351
Fragments arranged by size:
2845 1351 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2557 2638 2973 5117 2557
Size: 81 335 2144 2785
Fragments arranged by size:
2785 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 576 2153 3712 576
Size: 1577 1559 2209
Fragments arranged by size:
2209 1577 1559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1514 1682 1766 1850 1934 688
Size: 826 168 84 84 84 4099
Fragments arranged by size:
4099 826 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 1101 2521 3400 3892 4190 515
Size: 586 1420 879 492 298 1670
Fragments arranged by size:
1670 1420 879 586 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 345 1689 5049 345
Size: 1344 3360 641
Fragments arranged by size:
3360 1344 641
BsaAI yAC'GTr
Cuts at: 2698 2698
Size: 5345
BsaBI GATnn'nnATC
Cuts at: 6 875 2859 4896 6
Size: 869 1984 2037 455
Fragments arranged by size:
2037 1984 869 455
BsaHI Gr'CG_yC
Cuts at: 2776 3024 3726 5222 2776
Size: 248 702 1496 2899
Fragments arranged by size:
2899 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2248 2534 3055 3733 4007 4154 4565 2248
Size: 286 521 678 274 147 411 3028
Fragments arranged by size:
3028 678 521 411 286 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 975 1005 975
Size: 30 5315
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 568 665 937 568
Size: 97 272 4976
Fragments arranged by size:
4976 272 97
BseYI C'CCAG_C
Cuts at: 767 899 2770 3528 4105 4601 767
Size: 132 1871 758 577 496 1511
Fragments arranged by size:
1871 1511 758 577 496 132
BsiEI CG_ry'CG
Cuts at: 104 2887 2933 4141 104
Size: 2783 46 1208 1308
Fragments arranged by size:
2783 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 335 1632 2150 2359 3137 3327 4119 335
Size: 1297 518 209 778 190 792 1561
Fragments arranged by size:
1561 1297 792 778 518 209 190
Bsp1286I G_dGCh'C
Cuts at: 125 335 1632 2150 2359 2970 3063 3137 3327
Size: 210 1297 518 209 611 93 74 190
Cuts at: 3327 3389 4119 125
Size: 62 730 1351
Fragments arranged by size:
1351 1297 730 611 518 210 209 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4565 4565
Size: 5345
BspHI T'CATG_A
Cuts at: 4521 4521
Size: 5345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2395 2609 2911 3292 2395
Size: 214 302 381 4448
Fragments arranged by size:
4448 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3637 3691 5175 5340 50
Size: 3587 54 1484 165 55
Fragments arranged by size:
3587 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3257 3257
Size: 5345
BsrFI r'CCGG_y
Cuts at: 3343 3524 3733 3343
Size: 181 209 4955
Fragments arranged by size:
4955 209 181
BssSI C'ACGA_G
Cuts at: 3616 3974 3616
Size: 358 4987
BstAPI GCAn_nnn'nTGC
Cuts at: 2722 2722
Size: 5345
BstBI TT'CG_AA
Cuts at: 3706 3706
Size: 5345
BstEII G'GTnAC_C
Cuts at: 477 477
Size: 5345
BstYI r'GATC_y
Cuts at: 1 519 2370 2837 2860 3195 3441 4453 4539
Size: 518 1851 467 23 335 246 1012 86
Cuts at: 4539 4551 4897 1
Size: 12 346 449
Fragments arranged by size:
1851 1012 518 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 715 865 715
Size: 150 5195
BtgI C'CryG_G
Cuts at: 790 790
Size: 5345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1532 3295 3476 3639 1532
Size: 1763 181 163 3238
Fragments arranged by size:
3238 1763 181 163
BtsI GCAGTG_nn'
Cuts at: 2050 2588 2050
Size: 538 4807
DraI TTT'AAA
Cuts at: 2292 2344 2292
Size: 52 5293
DrdI GACnn_nn'nnGTC
Cuts at: 3051 3909 3051
Size: 858 4487
EaeI y'GGCC_r
Cuts at: 2930 3104 3495 3522 4867 2930
Size: 174 391 27 1345 3408
Fragments arranged by size:
3408 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2930 2930
Size: 5345
EarI CTCTTCn'nnn_
Cuts at: 951 1224 3368 3578 951
Size: 273 2144 210 2718
Fragments arranged by size:
2718 2144 273 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3553 3863 4009 5155 3553
Size: 310 146 1146 3743
Fragments arranged by size:
3743 1146 310 146
EcoICRI GAG'CTC
Cuts at: 333 2357 333
Size: 2024 3321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 1034 1107 1205 1514 1682 1766 1770 1850
Size: 346 73 98 309 168 84 4 80
Cuts at: 1850 1934 2106 3170 3602 4349 4617 688
Size: 84 172 1064 432 747 268 1416
Fragments arranged by size:
1416 1064 747 432 346 309 268 172
168 98 84 84 80 73 4
EcoO109I rG'GnC_Cy
Cuts at: 189 1271 2484 189
Size: 1082 1213 3050
Fragments arranged by size:
3050 1213 1082
EcoRI G'AATT_C
Cuts at: 1484 1652 1736 1820 1878 1904 2072 2156 2349
Size: 168 84 84 58 26 168 84 193
Cuts at: 2349 1484
Size: 4480
Fragments arranged by size:
4480 193 168 168 84 84 84 58
26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1137 3364 1137
Size: 2227 3118
FspI TGC'GCA
Cuts at: 3126 3126
Size: 5345
HaeII r_GCGC'y
Cuts at: 1463 2779 3027 4049 5028 1463
Size: 1316 248 1022 979 1780
Fragments arranged by size:
1780 1316 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 511 543 1531 1563 1699 1731 1965
Size: 32 241 32 988 32 136 32 234
Cuts at: 1965 1997 3187 3217 3219 3249 238
Size: 32 1190 30 2 30 2334
Fragments arranged by size:
2334 1190 988 241 234 136 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 322 2384 322
Size: 2062 3283
HindIII A'AGCT_T
Cuts at: 1529 1529
Size: 5345
KasI G'GCGC_C
Cuts at: 2775 3023 2775
Size: 248 5097
KpnI G_GTAC'C
Cuts at: 2365 2365
Size: 5345
MfeI C'AATT_G
Cuts at: 1770 1770
Size: 5345
MluI A'CGCG_T
Cuts at: 4778 4778
Size: 5345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1336 4016 4200 1336
Size: 2680 184 2481
Fragments arranged by size:
2680 2481 184
MscI TGG'CCA
Cuts at: 3106 3106
Size: 5345
MslI CAynn'nnrTG
Cuts at: 1929 2081 3334 3461 1929
Size: 152 1253 127 3813
Fragments arranged by size:
3813 1253 152 127
MspA1I CmG'CkG
Cuts at: 2456 2770 3130 4143 4388 2456
Size: 314 360 1013 245 3413
Fragments arranged by size:
3413 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3526 3526
Size: 5345
NarI GG'CG_CC
Cuts at: 2776 3024 2776
Size: 248 5097
NcoI C'CATG_G
Cuts at: 790 790
Size: 5345
NgoMIV G'CCGG_C
Cuts at: 3524 3524
Size: 5345
NspI r_CATG'y
Cuts at: 1766 2398 2898 3805 1766
Size: 632 500 907 3306
Fragments arranged by size:
3306 907 632 500
PacI TTA_AT'TAA
Cuts at: 1061 1061
Size: 5345
PciI A'CATG_T
Cuts at: 1762 3801 1762
Size: 2039 3306
PmeI GTTT'AAAC
Cuts at: 2344 2344
Size: 5345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 4484
Size: 32 5313
PpuMI rG'GwC_Cy
Cuts at: 189 1271 189
Size: 1082 4263
PsiI TTA'TAA
Cuts at: 1676 2096 1676
Size: 420 4925
PstI C_TGCA'G
Cuts at: 2337 2392 3077 2337
Size: 55 685 4605
Fragments arranged by size:
4605 685 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5345
PvuII CAG'CTG
Cuts at: 2770 3130 2770
Size: 360 4985
RsrII CG'GwC_CG
Cuts at: 3540 3540
Size: 5345
SacI G_AGCT'C
Cuts at: 335 2359 335
Size: 2024 3321
SalI G'TCGA_C
Cuts at: 2382 2382
Size: 5345
SapI GCTCTTCn'nnn_
Cuts at: 3368 3578 3368
Size: 210 5135
SbfI CC_TGCA'GG
Cuts at: 2392 2392
Size: 5345
ScaI AGT'ACT
Cuts at: 4862 4862
Size: 5345
SfcI C'TryA_G
Cuts at: 33 116 1455 2333 2388 3073 4066 4257 33
Size: 83 1339 878 55 685 993 191 1121
Fragments arranged by size:
1339 1121 993 878 685 191 83 55
SfoI GGC'GCC
Cuts at: 2777 3025 2777
Size: 248 5097
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
SmaI CCC'GGG
Cuts at: 2367 2367
Size: 5345
SmlI C'TyrA_G
Cuts at: 530 1080 2500 3415 3907 4169 530
Size: 550 1420 915 492 262 1706
Fragments arranged by size:
1706 1420 915 550 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5345
SphI G_CATG'C
Cuts at: 2398 2898 2398
Size: 500 4845
SspI AAT'ATT
Cuts at: 907 907
Size: 5345
StuI AGG'CCT
Cuts at: 2306 2306
Size: 5345
StyI C'CwwG_G
Cuts at: 790 2479 2833 790
Size: 1689 354 3302
Fragments arranged by size:
3302 1689 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2928 3061 3520 3729 2928
Size: 133 459 209 4544
Fragments arranged by size:
4544 459 209 133
TatI w'GTAC_w
Cuts at: 4860 4860
Size: 5345
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 573 605 4862 4894 573
Size: 32 4257 32 1024
Fragments arranged by size:
4257 1024 32 32
Tth111I GACn'n_nGTC
Cuts at: 3142 3142
Size: 5345
XbaI T'CTAG_A
Cuts at: 2376 2376
Size: 5345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 907 2840 907
Size: 1933 3412
XmaI C'CCGG_G
Cuts at: 2365 2365
Size: 5345
XmnI GAAnn'nnTTC
Cuts at: 1120 1588 1615 2035 2119 1120
Size: 468 27 420 84 4346
Fragments arranged by size:
4346 468 420 84 27
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PacI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI