Restriction map for
pF1KA1948
(Circular) (Six-base) MAPSORT of: pF1KA1948.seq Check: 9201 from: 1 to: 4709
pF1KA1948 4709 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 1747 1747
Size: 4709
Acc65I G'GTAC_C
Cuts at: 1725 1725
Size: 4709
AclI AA'CG_TT
Cuts at: 4549 4549
Size: 4709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 711 1383 2534 2966 3319 3981 504
Size: 207 672 1151 432 353 662 1232
Fragments arranged by size:
1232 1151 672 662 432 353 207
AflII C'TTAA_G
Cuts at: 442 442
Size: 4709
AflIII A'CryG_T
Cuts at: 2061 3861 4142 2061
Size: 1800 281 2628
Fragments arranged by size:
2628 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 2014 3452 2014
Size: 1438 3271
ApaLI G'TGCA_C
Cuts at: 3547 3547
Size: 4709
ApoI r'AATT_y
Cuts at: 396 677 1181 1265 1601 1685 1713 4062 4318
Size: 281 504 84 336 84 28 2349 256
Cuts at: 4318 396
Size: 787
Fragments arranged by size:
2349 787 504 336 281 256 84 84
28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 1729 1729
Size: 4709
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 507 540 507
Size: 33 4676
BamHI G'GATC_C
Cuts at: 1734 1734
Size: 4709
BanI G'GyrC_C
Cuts at: 1725 2139 2387 2422 1725
Size: 414 248 35 4012
Fragments arranged by size:
4012 414 248 35
BanII G_rGCy'C
Cuts at: 1723 2753 1723
Size: 1030 3679
BbeI G_GCGC'C
Cuts at: 2143 2391 2143
Size: 248 4461
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 4709
BciVI GTATCCnnnnn_n'
Cuts at: 150 508 2607 3663 4709 150
Size: 358 2099 1056 1046 150
Fragments arranged by size:
2099 1056 1046 358 150
BclI T'GATC_A
Cuts at: 2229 2229
Size: 4709
BfrBI ATG'CAT
Cuts at: 849 849
Size: 4709
BglII A'GATC_T
Cuts at: 825 2224 4261 825
Size: 1399 2037 1273
Fragments arranged by size:
2037 1399 1273
BlpI GC'TnA_GC
Cuts at: 1821 1821
Size: 4709
Bme1580I G_kGCm'C
Cuts at: 2334 2427 3551 2334
Size: 93 1124 3492
Fragments arranged by size:
3492 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 250 399 1921 2002 2337 4481 250
Size: 149 1522 81 335 2144 478
Fragments arranged by size:
2144 1522 478 335 149 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 140
Size: 4709
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 1885 2764 3478 3776 491
Size: 1394 879 714 298 1424
Fragments arranged by size:
1424 1394 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 240 337 349 454 495 4413 240
Size: 97 12 105 41 3918 536
Fragments arranged by size:
3918 536 105 97 41 12
BsaAI yAC'GTr
Cuts at: 2062 2062
Size: 4709
BsaBI GATnn'nnATC
Cuts at: 6 2223 4260 6
Size: 2217 2037 455
Fragments arranged by size:
2217 2037 455
BsaHI Gr'CG_yC
Cuts at: 2140 2388 3090 4586 2140
Size: 248 702 1496 2263
Fragments arranged by size:
2263 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1898 2419 3097 3508 3655 3929 1898
Size: 521 678 411 147 274 2678
Fragments arranged by size:
2678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 1154 1184 334
Size: 30 790 30 3859
Fragments arranged by size:
3859 790 30 30
BseYI C'CCAG_C
Cuts at: 2134 2892 3557 3965 2134
Size: 758 665 408 2878
Fragments arranged by size:
2878 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 842 722
Size: 120 4589
BsiEI CG_ry'CG
Cuts at: 104 2251 2297 3527 104
Size: 2147 46 1230 1286
Fragments arranged by size:
2147 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1723 2501 2691 3551 1723
Size: 778 190 860 2881
Fragments arranged by size:
2881 860 778 190
BsmI GAATG_Cn'
Cuts at: 1045 1138 1306 1390 1517 1045
Size: 93 168 84 127 4237
Fragments arranged by size:
4237 168 127 93 84
Bsp1286I G_dGCh'C
Cuts at: 1723 2334 2427 2501 2691 2753 3551 1723
Size: 611 93 74 190 62 798 2881
Fragments arranged by size:
2881 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3141 3141
Size: 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1759 1973 2275 2656 1759
Size: 214 302 381 3812
Fragments arranged by size:
3812 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3001 3055 4539 4704 50
Size: 2951 54 1484 165 55
Fragments arranged by size:
2951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2621 2621
Size: 4709
BsrFI r'CCGG_y
Cuts at: 2707 2888 2707
Size: 181 4528
BsrGI T'GTAC_A
Cuts at: 197 197
Size: 4709
BssSI C'ACGA_G
Cuts at: 2980 3688 2980
Size: 708 4001
BstAPI GCAn_nnn'nTGC
Cuts at: 2086 2086
Size: 4709
BstBI TT'CG_AA
Cuts at: 3070 3070
Size: 4709
BstXI CCAn_nnnn'nTGG
Cuts at: 164 164
Size: 4709
BstYI r'GATC_y
Cuts at: 1 825 1734 2201 2224 2559 2805 3111 3123
Size: 824 909 467 23 335 246 306 12
Cuts at: 3123 3209 4261 1
Size: 86 1052 449
Fragments arranged by size:
1052 909 824 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 435 1236 107
Size: 328 801 3580
Fragments arranged by size:
3580 801 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2659 2840 3003 2659
Size: 181 163 4365
Fragments arranged by size:
4365 181 163
BtsI GCAGTG_nn'
Cuts at: 1952 1952
Size: 4709
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1032 1067 1032
Size: 35 4674
DraI TTT'AAA
Cuts at: 458 1708 458
Size: 1250 3459
DrdI GACnn_nn'nnGTC
Cuts at: 2415 3759 2415
Size: 1344 3365
EaeI y'GGCC_r
Cuts at: 2294 2468 2859 2886 4231 2294
Size: 174 391 27 1345 2772
Fragments arranged by size:
2772 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2294 2294
Size: 4709
EarI CTCTTCn'nnn_
Cuts at: 155 2732 2942 155
Size: 2577 210 1922
Fragments arranged by size:
2577 1922 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2917 3659 3805 4519 2917
Size: 742 146 714 3107
Fragments arranged by size:
3107 742 714 146
EcoICRI GAG'CTC
Cuts at: 1721 1721
Size: 4709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 504 711 1383 2534 2966 3319 3981 140
Size: 364 207 672 1151 432 353 662 868
Fragments arranged by size:
1151 868 672 662 432 364 353 207
EcoNI CCTnn'n_nnAGG
Cuts at: 509 509
Size: 4709
EcoO109I rG'GnC_Cy
Cuts at: 1848 1848
Size: 4709
EcoRI G'AATT_C
Cuts at: 677 1181 1265 1601 1713 677
Size: 504 84 336 112 3673
Fragments arranged by size:
3673 504 336 112 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1665 2728 1665
Size: 1063 3646
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 2143 2391 3621 4392 2143
Size: 248 1230 771 2460
Fragments arranged by size:
2460 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 990 1022 2551 2581 2583 2613 457
Size: 32 501 32 1529 30 2 30 2553
Fragments arranged by size:
2553 1529 501 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1748 1748
Size: 4709
HindIII A'AGCT_T
Cuts at: 806 1012 806
Size: 206 4503
KasI G'GCGC_C
Cuts at: 2139 2387 2139
Size: 248 4461
KpnI G_GTAC'C
Cuts at: 1729 1729
Size: 4709
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3468 3652 194
Size: 3274 184 1251
Fragments arranged by size:
3274 1251 184
MscI TGG'CCA
Cuts at: 2470 2470
Size: 4709
MslI CAynn'nnrTG
Cuts at: 243 686 770 854 938 1106 1190 1274 1358
Size: 443 84 84 84 168 84 84 84
Cuts at: 1358 1442 1610 2698 2825 243
Size: 84 168 1088 127 2127
Fragments arranged by size:
2127 1088 443 168 168 127 84 84
84 84 84 84 84
MspA1I CmG'CkG
Cuts at: 1820 2134 2494 3278 3523 1820
Size: 314 360 784 245 3006
Fragments arranged by size:
3006 784 360 314 245
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 4709
NarI GG'CG_CC
Cuts at: 2140 2388 2140
Size: 248 4461
NcoI C'CATG_G
Cuts at: 107 435 107
Size: 328 4381
NdeI CA'TA_TG
Cuts at: 814 814
Size: 4709
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 4709
NsiI A_TGCA'T
Cuts at: 851 851
Size: 4709
NspI r_CATG'y
Cuts at: 1762 2262 3865 1762
Size: 500 1603 2606
Fragments arranged by size:
2606 1603 500
PciI A'CATG_T
Cuts at: 3861 3861
Size: 4709
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 4677
PsiI TTA'TAA
Cuts at: 1457 1457
Size: 4709
PstI C_TGCA'G
Cuts at: 1756 2441 1756
Size: 685 4024
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4709
PvuII CAG'CTG
Cuts at: 2134 2494 2134
Size: 360 4349
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 1723 1723
Size: 4709
SalI G'TCGA_C
Cuts at: 1746 1746
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 1756 1756
Size: 4709
ScaI AGT'ACT
Cuts at: 4226 4226
Size: 4709
SfcI C'TryA_G
Cuts at: 33 222 1752 2437 3405 3596 33
Size: 189 1530 685 968 191 1146
Fragments arranged by size:
1530 1146 968 685 191 189
SfoI GGC'GCC
Cuts at: 2141 2389 2141
Size: 248 4461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 1731 1731
Size: 4709
SmlI C'TyrA_G
Cuts at: 442 506 1864 2779 3493 3755 442
Size: 64 1358 915 714 262 1396
Fragments arranged by size:
1396 1358 915 714 262 64
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 1762 2262 1762
Size: 500 4209
StuI AGG'CCT
Cuts at: 1060 1144 1396 1480 1564 1648 1060
Size: 84 252 84 84 84 4121
Fragments arranged by size:
4121 252 84 84 84 84
StyI C'CwwG_G
Cuts at: 107 298 435 1843 2197 107
Size: 191 137 1408 354 2619
Fragments arranged by size:
2619 1408 354 191 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2292 2425 2884 3093 2292
Size: 133 459 209 3908
Fragments arranged by size:
3908 459 209 133
TatI w'GTAC_w
Cuts at: 197 834 1086 4224 197
Size: 637 252 3138 682
Fragments arranged by size:
3138 682 637 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 4677
Tth111I GACn'n_nGTC
Cuts at: 2506 2506
Size: 4709
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2204 2204
Size: 4709
XmaI C'CCGG_G
Cuts at: 1729 1729
Size: 4709
XmnI GAAnn'nnTTC
Cuts at: 640 1396 1605 640
Size: 756 209 3744
Fragments arranged by size:
3744 756 209
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BspEI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI