Restriction map for
pF1KA1938
(Circular) (Six-base) MAPSORT of: pF1KA1938.seq Check: 4753 from: 1 to: 7142
pF1KA1938 7142 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AccI GT'mk_AC
Cuts at: 887 1526 1685 4180 887
Size: 639 159 2495 3849
Fragments arranged by size:
3849 2495 639 159
Acc65I G'GTAC_C
Cuts at: 1193 4158 1193
Size: 2965 4177
AclI AA'CG_TT
Cuts at: 6982 6982
Size: 7142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 1822 2254 2398 3514 4967 5399 6146 6414
Size: 1669 432 144 1116 1453 432 747 268
Cuts at: 6414 153
Size: 881
Fragments arranged by size:
1669 1453 1116 881 747 432 432 268
144
AflIII A'CryG_T
Cuts at: 3737 4494 5598 6575 3737
Size: 757 1104 977 4304
Fragments arranged by size:
4304 1104 977 757
AgeI A'CCGG_T
Cuts at: 1529 5530 1529
Size: 4001 3141
AhdI GACnn_n'nnGTC
Cuts at: 361 2415 361
Size: 2054 5088
AleI CACnn'nnGTG
Cuts at: 3008 3008
Size: 7142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 364 3922 364
Size: 3558 3584
AlwNI CAG_nnn'CTG
Cuts at: 585 2004 2868 3253 3559 4447 6014 585
Size: 1419 864 385 306 888 1567 1713
Fragments arranged by size:
1713 1567 1419 888 864 385 306
ApaI G_GGCC'C
Cuts at: 621 621
Size: 7142
ApaLI G'TGCA_C
Cuts at: 733 1454 1698 5912 733
Size: 721 244 4214 1963
Fragments arranged by size:
4214 1963 721 244
ApoI r'AATT_y
Cuts at: 1642 4146 6495 6751 1642
Size: 2504 2349 256 2033
Fragments arranged by size:
2504 2349 2033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7142
AvaI C'yCGr_G
Cuts at: 119 261 420 920 1260 1791 2429 4162 119
Size: 142 159 500 340 531 638 1733 3099
Fragments arranged by size:
3099 1733 638 531 500 340 159 142
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 936 969 936
Size: 33 7109
BamHI G'GATC_C
Cuts at: 4167 4167
Size: 7142
BanI G'GyrC_C
Cuts at: 466 1172 1193 4158 4572 4820 4855 466
Size: 706 21 2965 414 248 35 2753
Fragments arranged by size:
2965 2753 706 414 248 35 21
BanII G_rGCy'C
Cuts at: 621 701 959 1261 1418 2174 3020 4156 5186
Size: 80 258 302 157 756 846 1136 1030
Cuts at: 5186 621
Size: 2577
Fragments arranged by size:
2577 1136 1030 846 756 302 258 157
80
BbeI G_GCGC'C
Cuts at: 1176 4576 4824 1176
Size: 3400 248 3494
Fragments arranged by size:
3494 3400 248
BbsI GAAGACnn'nnnn_
Cuts at: 1017 1017
Size: 7142
BbvCI CC'TCA_GC
Cuts at: 686 2336 2824 3433 686
Size: 1650 488 609 4395
Fragments arranged by size:
4395 1650 609 488
BciVI GTATCCnnnnn_n'
Cuts at: 5040 5801 7142 5040
Size: 761 1341 5040
Fragments arranged by size:
5040 1341 761
BclI T'GATC_A
Cuts at: 4662 4662
Size: 7142
BglI GCCn_nnn'nGGC
Cuts at: 486 1165 486
Size: 679 6463
BglII A'GATC_T
Cuts at: 2105 4657 6694 2105
Size: 2552 2037 2553
Fragments arranged by size:
2553 2552 2037
BlpI GC'TnA_GC
Cuts at: 2215 3950 4254 2215
Size: 1735 304 5103
Fragments arranged by size:
5103 1735 304
Bme1580I G_kGCm'C
Cuts at: 471 621 737 1074 1458 1702 3621 4767 4860
Size: 150 116 337 384 244 1919 1146 93
Cuts at: 4860 5916 471
Size: 1056 1697
Fragments arranged by size:
1919 1697 1146 1056 384 337 244 150
116 93
BmrI ACTGGGnnnn_n'
Cuts at: 1916 4354 4435 4770 6914 1916
Size: 2438 81 335 2144 2144
Fragments arranged by size:
2438 2144 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 134 666 3087 3732 5509 134
Size: 532 2421 645 1777 1767
Fragments arranged by size:
2421 1777 1767 645 532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2086 3163 3275 3850 2086
Size: 1077 112 575 5378
Fragments arranged by size:
5378 1077 575 112
Bpu10I CC'TnA_GC
Cuts at: 505 686 1272 2336 2824 3433 3850 3989 505
Size: 181 586 1064 488 609 417 139 3658
Fragments arranged by size:
3658 1064 609 586 488 417 181 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2706 3631 4318 5197 5689 5987 1609
Size: 1097 925 687 879 492 298 2764
Fragments arranged by size:
2764 1097 925 879 687 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 122 3585 6846 122
Size: 3463 3261 418
Fragments arranged by size:
3463 3261 418
BsaAI yAC'GTr
Cuts at: 4495 4495
Size: 7142
BsaBI GATnn'nnATC
Cuts at: 6 4656 6693 6
Size: 4650 2037 455
Fragments arranged by size:
4650 2037 455
BsaHI Gr'CG_yC
Cuts at: 1173 2775 4573 4821 5523 7019 1173
Size: 1602 1798 248 702 1496 1296
Fragments arranged by size:
1798 1602 1496 1296 702 248
BsaWI w'CCGG_w
Cuts at: 320 560 1529 3746 4331 4852 5530 5804 5951
Size: 240 969 2217 585 521 678 274 147
Cuts at: 5951 6362 320
Size: 411 1100
Fragments arranged by size:
2217 1100 969 678 585 521 411 274
240 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 417 447 417
Size: 30 7112
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 339 438 1456 2248 3718 3721 3931 3934 339
Size: 99 1018 792 1470 3 210 3 3547
Fragments arranged by size:
3547 1470 1018 792 210 99 3 3
BseYI C'CCAG_C
Cuts at: 1168 1905 2210 2238 2861 4068 4567 5325 5902
Size: 737 305 28 623 1207 499 758 577
Cuts at: 5902 6398 1168
Size: 496 1912
Fragments arranged by size:
1912 1207 758 737 623 577 499 496
305 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 1424 2553 2701 3718 701
Size: 723 1129 148 1017 4125
Fragments arranged by size:
4125 1129 1017 723 148
BsiEI CG_ry'CG
Cuts at: 104 818 2702 4684 4730 5938 104
Size: 714 1884 1982 46 1208 1308
Fragments arranged by size:
1982 1884 1308 1208 714 46
BsiHKAI G_wGCw'C
Cuts at: 701 737 959 1040 1458 1550 1656 1702 1724
Size: 36 222 81 418 92 106 46 22
Cuts at: 1724 2174 4156 4934 5124 5916 701
Size: 450 1982 778 190 792 1927
Fragments arranged by size:
1982 1927 792 778 450 418 222 190
106 92 81 46 36 22
BsmI GAATG_Cn'
Cuts at: 3516 3516
Size: 7142
BsmBI CGTCTCn'nnnn_
Cuts at: 348 348
Size: 7142
Bsp1286I G_dGCh'C
Cuts at: 471 621 701 737 959 1040 1074 1261 1418
Size: 150 80 36 222 81 34 187 157
Cuts at: 1418 1458 1550 1656 1702 1724 2174 3020 3621
Size: 40 92 106 46 22 450 846 601
Cuts at: 3621 4156 4767 4860 4934 5124 5186 5916 471
Size: 535 611 93 74 190 62 730 1697
Fragments arranged by size:
1697 846 730 611 601 535 450 222
190 187 157 150 106 93 92 81
80 74 62 46 40 36 34 22
BspEI T'CCGG_A
Cuts at: 6362 6362
Size: 7142
BspHI T'CATG_A
Cuts at: 654 2051 6318 654
Size: 1397 4267 1478
Fragments arranged by size:
4267 1478 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 104 1064 1742 2720 3197 4192 4406 4708 5089
Size: 960 678 978 477 995 214 302 381
Cuts at: 5089 104
Size: 2157
Fragments arranged by size:
2157 995 978 960 678 477 381 302
214
BsrBI CCG'CTC
Cuts at: 50 1004 2325 3266 3564 3630 3729 3768 5434
Size: 954 1321 941 298 66 99 39 1666
Cuts at: 5434 5488 6972 7137 50
Size: 54 1484 165 55
Fragments arranged by size:
1666 1484 1321 954 941 298 165 99
66 55 54 39
BsrDI GCAATG_nn'
Cuts at: 1982 3307 5054 1982
Size: 1325 1747 4070
Fragments arranged by size:
4070 1747 1325
BsrFI r'CCGG_y
Cuts at: 372 1059 1529 3677 5140 5321 5530 372
Size: 687 470 2148 1463 181 209 1984
Fragments arranged by size:
2148 1984 1463 687 470 209 181
BssSI C'ACGA_G
Cuts at: 2571 5413 5771 2571
Size: 2842 358 3942
Fragments arranged by size:
3942 2842 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2607 3198 4519 2607
Size: 591 1321 5230
Fragments arranged by size:
5230 1321 591
BstBI TT'CG_AA
Cuts at: 2689 5503 2689
Size: 2814 4328
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 3010 2791
Size: 219 6923
BstYI r'GATC_y
Cuts at: 1 581 1225 2105 4167 4634 4657 4992 5238
Size: 580 644 880 2062 467 23 335 246
Cuts at: 5238 6250 6336 6348 6694 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2062 1012 880 644 580 467 449 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3246 3246
Size: 7142
BtgI C'CryG_G
Cuts at: 390 1346 2940 2958 3045 3418 3944 4048 390
Size: 956 1594 18 87 373 526 104 3484
Fragments arranged by size:
3484 1594 956 526 373 104 87 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 166 1842 5092 5273 5436 166
Size: 1676 3250 181 163 1872
Fragments arranged by size:
3250 1872 1676 181 163
BtsI GCAGTG_nn'
Cuts at: 684 1195 1224 1779 2179 2245 2688 4385 684
Size: 511 29 555 400 66 443 1697 3441
Fragments arranged by size:
3441 1697 555 511 443 400 66 29
ClaI AT'CG_AT
Cuts at: 3664 3664
Size: 7142
DraI TTT'AAA
Cuts at: 4141 4141
Size: 7142
DraIII CAC_nnn'GTG
Cuts at: 1785 1785
Size: 7142
DrdI GACnn_nn'nnGTC
Cuts at: 1610 4848 5706 1610
Size: 3238 858 3046
Fragments arranged by size:
3238 3046 858
EaeI y'GGCC_r
Cuts at: 815 1157 1511 2006 2664 2699 2937 2955 3361
Size: 342 354 495 658 35 238 18 406
Cuts at: 3361 4727 4901 5292 5319 6664 815
Size: 1366 174 391 27 1345 1293
Fragments arranged by size:
1366 1345 1293 658 495 406 391 354
342 238 174 35 27 18
EagI C'GGCC_G
Cuts at: 815 2699 4727 815
Size: 1884 2028 3230
Fragments arranged by size:
3230 2028 1884
EarI CTCTTCn'nnn_
Cuts at: 424 1586 1891 2905 3078 3629 3752 3838 3967
Size: 1162 305 1014 173 551 123 86 129
Cuts at: 3967 5165 5375 424
Size: 1198 210 2191
Fragments arranged by size:
2191 1198 1162 1014 551 305 210 173
129 123 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3491 5350 5660 5806 6952 3491
Size: 1859 310 146 1146 3681
Fragments arranged by size:
3681 1859 1146 310 146
EcoICRI GAG'CTC
Cuts at: 699 957 2172 4154 699
Size: 258 1215 1982 3687
Fragments arranged by size:
3687 1982 1215 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 1822 2086 2254 2398 3163 3275 3514 3850
Size: 1669 264 168 144 765 112 239 336
Cuts at: 3850 4967 5399 6146 6414 153
Size: 1117 432 747 268 881
Fragments arranged by size:
1669 1117 881 765 747 432 336 268
264 239 168 144 112
EcoNI CCTnn'n_nnAGG
Cuts at: 751 1762 2782 3436 751
Size: 1011 1020 654 4457
Fragments arranged by size:
4457 1020 1011 654
EcoO109I rG'GnC_Cy
Cuts at: 184 2391 2709 2926 3066 3081 3250 4281 184
Size: 2207 318 217 140 15 169 1031 3045
Fragments arranged by size:
3045 2207 1031 318 217 169 140 15
EcoRI G'AATT_C
Cuts at: 1642 4146 1642
Size: 2504 4638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2055 5161 2055
Size: 3106 4036
FspI TGC'GCA
Cuts at: 1836 4923 1836
Size: 3087 4055
HaeII r_GCGC'y
Cuts at: 1176 1880 4576 4824 5846 6825 1176
Size: 704 2696 248 1022 979 1493
Fragments arranged by size:
2696 1493 1022 979 704 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 347 379 573 605 825 857 1631
Size: 32 202 32 194 32 220 32 774
Cuts at: 1631 1663 2097 2129 2401 2433 3801 3833 4984
Size: 32 434 32 272 32 1368 32 1151
Cuts at: 4984 5014 5016 5046 113
Size: 30 2 30 2209
Fragments arranged by size:
2209 1368 1151 774 434 272 220 202
194 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1773 2619 3335 4181 1773
Size: 846 716 846 4734
Fragments arranged by size:
4734 846 846 716
HindIII A'AGCT_T
Cuts at: 633 633
Size: 7142
KasI G'GCGC_C
Cuts at: 1172 4572 4820 1172
Size: 3400 248 3494
Fragments arranged by size:
3494 3400 248
KpnI G_GTAC'C
Cuts at: 1197 4162 1197
Size: 2965 4177
MluI A'CGCG_T
Cuts at: 6575 6575
Size: 7142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 217 3608 3767 4042 5813 5997 217
Size: 3391 159 275 1771 184 1362
Fragments arranged by size:
3391 1771 1362 275 184 159
MscI TGG'CCA
Cuts at: 1159 1513 2008 2957 4903 1159
Size: 354 495 949 1946 3398
Fragments arranged by size:
3398 1946 949 495 354
MslI CAynn'nnrTG
Cuts at: 653 1513 3008 5131 5258 653
Size: 860 1495 2123 127 2537
Fragments arranged by size:
2537 2123 1495 860 127
MspA1I CmG'CkG
Cuts at: 273 685 849 3278 3371 3461 3470 3957 4253
Size: 412 164 2429 93 90 9 487 296
Cuts at: 4253 4567 4927 5940 6185 273
Size: 314 360 1013 245 1230
Fragments arranged by size:
2429 1230 1013 487 412 360 314 296
245 164 93 90 9
NaeI GCC'GGC
Cuts at: 1061 3679 5323 1061
Size: 2618 1644 2880
Fragments arranged by size:
2880 2618 1644
NarI GG'CG_CC
Cuts at: 1173 4573 4821 1173
Size: 3400 248 3494
Fragments arranged by size:
3494 3400 248
NcoI C'CATG_G
Cuts at: 1346 2940 2958 3045 3944 1346
Size: 1594 18 87 899 4544
Fragments arranged by size:
4544 1594 899 87 18
NgoMIV G'CCGG_C
Cuts at: 1059 3677 5321 1059
Size: 2618 1644 2880
Fragments arranged by size:
2880 2618 1644
NspI r_CATG'y
Cuts at: 975 2188 2820 3201 3591 3741 4195 4695 5602
Size: 1213 632 381 390 150 454 500 907
Cuts at: 5602 975
Size: 2515
Fragments arranged by size:
2515 1213 907 632 500 454 390 381
150
PasI CC'CwG_GG
Cuts at: 439 2784 3022 439
Size: 2345 238 4559
Fragments arranged by size:
4559 2345 238
PciI A'CATG_T
Cuts at: 3737 5598 3737
Size: 1861 5281
PflMI CCAn_nnn'nTGG
Cuts at: 2004 2503 3046 3403 2004
Size: 499 543 357 5743
Fragments arranged by size:
5743 543 499 357
PfoI T'CCnGG_A
Cuts at: 314 3179 314
Size: 2865 4277
PmeI GTTT'AAAC
Cuts at: 4141 4141
Size: 7142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6281 6313 6281
Size: 32 7110
PpuMI rG'GwC_Cy
Cuts at: 184 2391 2709 2926 3066 3081 184
Size: 2207 318 217 140 15 4245
Fragments arranged by size:
4245 2207 318 217 140 15
PspOMI G'GGCC_C
Cuts at: 617 617
Size: 7142
PspXI vC'TCGA_Gb
Cuts at: 420 2429 420
Size: 2009 5133
PstI C_TGCA'G
Cuts at: 848 1475 3194 4097 4189 4874 848
Size: 627 1719 903 92 685 3116
Fragments arranged by size:
3116 1719 903 685 627 92
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7142
PvuII CAG'CTG
Cuts at: 273 685 4567 4927 273
Size: 412 3882 360 2488
Fragments arranged by size:
3882 2488 412 360
RsrII CG'GwC_CG
Cuts at: 5337 5337
Size: 7142
SacI G_AGCT'C
Cuts at: 701 959 2174 4156 701
Size: 258 1215 1982 3687
Fragments arranged by size:
3687 1982 1215 258
SalI G'TCGA_C
Cuts at: 4179 4179
Size: 7142
SanDI GG'GwC_CC
Cuts at: 184 3081 184
Size: 2897 4245
SapI GCTCTTCn'nnn_
Cuts at: 5165 5375 5165
Size: 210 6932
SbfI CC_TGCA'GG
Cuts at: 4189 4189
Size: 7142
ScaI AGT'ACT
Cuts at: 3655 6659 3655
Size: 3004 4138
SfcI C'TryA_G
Cuts at: 33 844 1471 3051 3123 3190 4093 4185 4870
Size: 811 627 1580 72 67 903 92 685
Cuts at: 4870 5863 6054 33
Size: 993 191 1121
Fragments arranged by size:
1580 1121 993 903 811 685 627 191
92 72 67
SfoI GGC'GCC
Cuts at: 1174 4574 4822 1174
Size: 3400 248 3494
Fragments arranged by size:
3494 3400 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7142
SmaI CCC'GGG
Cuts at: 922 4164 922
Size: 3242 3900
SmlI C'TyrA_G
Cuts at: 119 420 1624 2429 2721 3646 4297 5212 5704
Size: 301 1204 805 292 925 651 915 492
Cuts at: 5704 5966 119
Size: 262 1295
Fragments arranged by size:
1295 1204 925 915 805 651 492 301
292 262
SpeI A'CTAG_T
Cuts at: 67 1458 67
Size: 1391 5751
SphI G_CATG'C
Cuts at: 2188 2820 4195 4695 2188
Size: 632 1375 500 4635
Fragments arranged by size:
4635 1375 632 500
StuI AGG'CCT
Cuts at: 2435 2731 2435
Size: 296 6846
StyI C'CwwG_G
Cuts at: 1346 1490 1988 2477 2747 2940 2958 3045 3944
Size: 144 498 489 270 193 18 87 899
Cuts at: 3944 4010 4276 4630 1346
Size: 66 266 354 3858
Fragments arranged by size:
3858 899 498 489 354 270 266 193
144 87 66 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 797 2824 4725 4858 5317 5526 797
Size: 2027 1901 133 459 209 2413
Fragments arranged by size:
2413 2027 1901 459 209 133
TatI w'GTAC_w
Cuts at: 452 1477 3638 3653 6657 452
Size: 1025 2161 15 3004 937
Fragments arranged by size:
3004 2161 1025 937 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 417 449 1386 1418 3187 3210 3219 3242 6659
Size: 32 937 32 1769 23 9 23 3417
Cuts at: 6659 6691 417
Size: 32 868
Fragments arranged by size:
3417 1769 937 868 32 32 32 23
23 9
Tth111I GACn'n_nGTC
Cuts at: 1021 2851 4939 1021
Size: 1830 2088 3224
Fragments arranged by size:
3224 2088 1830
XbaI T'CTAG_A
Cuts at: 4173 4173
Size: 7142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 3220 3750 4637 3007
Size: 213 530 887 5512
Fragments arranged by size:
5512 887 530 213
XhoI C'TCGA_G
Cuts at: 119 420 2429 119
Size: 301 2009 4832
Fragments arranged by size:
4832 2009 301
XmaI C'CCGG_G
Cuts at: 920 4162 920
Size: 3242 3900
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BcgI
BfrBI BmgBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI