Restriction map for
pF1KA1926
(Circular) (Six-base) MAPSORT of: pF1KA1926.seq Check: 2743 from: 1 to: 4319
pF1KA1926 4319 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 261 261
Size: 4319
AatII G_ACGT'C
Cuts at: 401 401
Size: 4319
AccI GT'mk_AC
Cuts at: 1190 1357 1190
Size: 167 4152
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 2144 2576 2929 3591 961
Size: 1183 432 353 662 1689
Fragments arranged by size:
1689 1183 662 432 353
AflIII A'CryG_T
Cuts at: 1671 3471 3752 1671
Size: 1800 281 2238
Fragments arranged by size:
2238 1800 281
AleI CACnn'nnGTG
Cuts at: 287 614 287
Size: 327 3992
AlwNI CAG_nnn'CTG
Cuts at: 184 265 1624 3062 184
Size: 81 1359 1438 1441
Fragments arranged by size:
1441 1438 1359 81
ApaLI G'TGCA_C
Cuts at: 217 3157 217
Size: 2940 1379
ApoI r'AATT_y
Cuts at: 1323 3672 3928 1323
Size: 2349 256 1714
Fragments arranged by size:
2349 1714 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 119 785 1339 119
Size: 666 554 3099
Fragments arranged by size:
3099 666 554
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 292 1224 1335 1749 1997 2032 292
Size: 932 111 414 248 35 2579
Fragments arranged by size:
2579 932 414 248 111 35
BanII G_rGCy'C
Cuts at: 244 794 1333 2363 244
Size: 550 539 1030 2200
Fragments arranged by size:
2200 1030 550 539
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BbsI GAAGACnn'nnnn_
Cuts at: 393 393
Size: 4319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1069 1103 1137 1273 1307 1069
Size: 34 34 136 34 4081
Fragments arranged by size:
4081 136 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2217 3273 4319 2217
Size: 1056 1046 2217
Fragments arranged by size:
2217 1056 1046
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 455 1431 455
Size: 976 3343
Bme1580I G_kGCm'C
Cuts at: 221 1944 2037 3161 221
Size: 1723 93 1124 1379
Fragments arranged by size:
1723 1379 1124 93
BmgBI CAC'GTC
Cuts at: 911 1311 911
Size: 400 3919
BmrI ACTGGGnnnn_n'
Cuts at: 1531 1612 1947 4091 1531
Size: 81 335 2144 1759
Fragments arranged by size:
2144 1759 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 258 2686 258
Size: 2428 1891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1190 247
Size: 943 3376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1495 2374 3088 3386 343
Size: 1152 879 714 298 1276
Fragments arranged by size:
1276 1152 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4023 4023
Size: 4319
BsaAI yAC'GTr
Cuts at: 222 1672 222
Size: 1450 2869
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 164 350 398 896 1166 1187 1750 1998 2700
Size: 186 48 498 270 21 563 248 702
Cuts at: 2700 4196 164
Size: 1496 287
Fragments arranged by size:
1496 702 563 498 287 270 248 186
48 21
BsaWI w'CCGG_w
Cuts at: 967 1215 1508 2029 2707 3118 3265 3539 967
Size: 248 293 521 678 411 147 274 1747
Fragments arranged by size:
1747 678 521 411 293 274 248 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 220
Size: 30 4289
BseYI C'CCAG_C
Cuts at: 419 1235 1241 1247 1265 1744 2502 3167 3575
Size: 816 6 6 18 479 758 665 408
Cuts at: 3575 419
Size: 1163
Fragments arranged by size:
1163 816 758 665 479 408 18 6
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 842 1044 1291 685
Size: 157 202 247 3713
Fragments arranged by size:
3713 247 202 157
BsiEI CG_ry'CG
Cuts at: 104 1004 1861 1907 3137 104
Size: 900 857 46 1230 1286
Fragments arranged by size:
1286 1230 900 857 46
BsiHKAI G_wGCw'C
Cuts at: 221 1215 1333 2111 2301 3161 221
Size: 994 118 778 190 860 1379
Fragments arranged by size:
1379 994 860 778 190 118
BsmI GAATG_Cn'
Cuts at: 430 430
Size: 4319
Bsp1286I G_dGCh'C
Cuts at: 221 244 794 1215 1333 1944 2037 2111 2301
Size: 23 550 421 118 611 93 74 190
Cuts at: 2301 2363 3161 221
Size: 62 798 1379
Fragments arranged by size:
1379 798 611 550 421 190 118 93
74 62 23
BspEI T'CCGG_A
Cuts at: 967 967
Size: 4319
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 261 926 1369 1583 1885 2266 261
Size: 665 443 214 302 381 2314
Fragments arranged by size:
2314 665 443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2611 2665 4149 4314 50
Size: 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 2231
Size: 4319
BsrFI r'CCGG_y
Cuts at: 779 2317 2498 779
Size: 1538 181 2600
Fragments arranged by size:
2600 1538 181
BssHII G'CGCG_C
Cuts at: 140 654 976 978 1090 1117 1277 1279 140
Size: 514 322 2 112 27 160 2 3180
Fragments arranged by size:
3180 514 322 160 112 27 2 2
BssSI C'ACGA_G
Cuts at: 2590 3298 2590
Size: 708 3611
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 1696
Size: 4319
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 289 552 616 289
Size: 263 64 3992
Fragments arranged by size:
3992 263 64
BstYI r'GATC_y
Cuts at: 1 1344 1811 1834 2169 2415 2721 2733 2819
Size: 1343 467 23 335 246 306 12 86
Cuts at: 2819 3871 1
Size: 1052 449
Fragments arranged by size:
1343 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 152 285 336 444 615 1004 1064 107
Size: 45 133 51 108 171 389 60 3362
Fragments arranged by size:
3362 389 171 133 108 60 51 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 1092 1562 1092
Size: 470 3849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 356 391 356
Size: 35 4284
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 3369 2025
Size: 1344 2975
EaeI y'GGCC_r
Cuts at: 201 1001 1007 1076 1904 2078 2469 2496 3841
Size: 800 6 69 828 174 391 27 1345
Cuts at: 3841 201
Size: 679
Fragments arranged by size:
1345 828 800 679 391 174 69 27
6
EagI C'GGCC_G
Cuts at: 1001 1904 1001
Size: 903 3416
EarI CTCTTCn'nnn_
Cuts at: 1186 2342 2552 1186
Size: 1156 210 2953
Fragments arranged by size:
2953 1156 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 3269 3415 4129 2527
Size: 742 146 714 2717
Fragments arranged by size:
2717 742 714 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 961 1190 2144 2576 2929 3591 247
Size: 714 229 954 432 353 662 975
Fragments arranged by size:
975 954 714 662 432 353 229
EcoO109I rG'GnC_Cy
Cuts at: 415 849 1458 415
Size: 434 609 3276
Fragments arranged by size:
3276 609 434
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 654 2338 654
Size: 1684 2635
FspI TGC'GCA
Cuts at: 856 1147 2100 856
Size: 291 953 3075
Fragments arranged by size:
3075 953 291
HaeII r_GCGC'y
Cuts at: 265 528 1753 2001 3231 4002 265
Size: 263 1225 248 1230 771 582
Fragments arranged by size:
1230 1225 771 582 263 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 2161 2191 2193 2223 220
Size: 32 1909 30 2 30 2316
Fragments arranged by size:
2316 1909 32 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 938 3078 3262 938
Size: 2140 184 1995
Fragments arranged by size:
2140 1995 184
MscI TGG'CCA
Cuts at: 1009 2080 1009
Size: 1071 3248
MslI CAynn'nnrTG
Cuts at: 287 614 2308 2435 287
Size: 327 1694 127 2171
Fragments arranged by size:
2171 1694 327 127
MspA1I CmG'CkG
Cuts at: 154 384 699 948 1023 1047 1058 1066 1430
Size: 230 315 249 75 24 11 8 364
Cuts at: 1430 1744 2104 2888 3133 154
Size: 314 360 784 245 1340
Fragments arranged by size:
1340 784 364 360 315 314 249 245
230 75 24 11 8
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NcoI C'CATG_G
Cuts at: 107 107
Size: 4319
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NspI r_CATG'y
Cuts at: 1372 1872 3475 1372
Size: 500 1603 2216
Fragments arranged by size:
2216 1603 500
PciI A'CATG_T
Cuts at: 3471 3471
Size: 4319
PfoI T'CCnGG_A
Cuts at: 1160 1206 1160
Size: 46 4273
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PmlI CAC'GTG
Cuts at: 222 222
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 4287
PstI C_TGCA'G
Cuts at: 464 693 1046 1366 2051 464
Size: 229 353 320 685 2732
Fragments arranged by size:
2732 685 353 320 229
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 699 1023 1047 1744 2104 699
Size: 324 24 697 360 2914
Fragments arranged by size:
2914 697 360 324 24
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SacII CC_GC'GG
Cuts at: 155 1067 155
Size: 912 3407
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 1186 2342 2552 1186
Size: 1156 210 2953
Fragments arranged by size:
2953 1156 210
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 460 689 1042 1362 2047 3015 3206 33
Size: 427 229 353 320 685 968 191 1146
Fragments arranged by size:
1146 968 685 427 353 320 229 191
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 787 1341 787
Size: 554 3765
SmlI C'TyrA_G
Cuts at: 358 1474 2389 3103 3365 358
Size: 1116 915 714 262 1312
Fragments arranged by size:
1312 1116 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StuI AGG'CCT
Cuts at: 1137 1137
Size: 4319
StyI C'CwwG_G
Cuts at: 107 1010 1453 1807 107
Size: 903 443 354 2619
Fragments arranged by size:
2619 903 443 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 121 1902 2035 2494 2703 121
Size: 1781 133 459 209 1737
Fragments arranged by size:
1781 1737 459 209 133
TatI w'GTAC_w
Cuts at: 3834 3834
Size: 4319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 804 836 3836 3868 804
Size: 32 3000 32 1255
Fragments arranged by size:
3000 1255 32 32
Tth111I GACn'n_nGTC
Cuts at: 349 2116 349
Size: 1767 2552
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1814 1814
Size: 4319
XmaI C'CCGG_G
Cuts at: 785 1339 785
Size: 554 3765
ZraI GAC'GTC
Cuts at: 399 399
Size: 4319
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I BseRI
BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI