Restriction map for
pF1KA1880
(Circular) (Six-base) MAPSORT of: pF1KA1880.seq Check: 2439 from: 1 to: 4949
pF1KA1880 4949 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AatII G_ACGT'C
Cuts at: 221 221
Size: 4949
AccI GT'mk_AC
Cuts at: 1987 1987
Size: 4949
Acc65I G'GTAC_C
Cuts at: 1965 1965
Size: 4949
AclI AA'CG_TT
Cuts at: 243 4789 243
Size: 4546 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 815 2774 3206 3953 4221 350
Size: 465 1959 432 747 268 1078
Fragments arranged by size:
1959 1078 747 465 432 268
AflIII A'CryG_T
Cuts at: 1492 2301 3405 4382 1492
Size: 809 1104 977 2059
Fragments arranged by size:
2059 1104 977 809
AgeI A'CCGG_T
Cuts at: 3337 3337
Size: 4949
AleI CACnn'nnGTG
Cuts at: 226 226
Size: 4949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 294 294
Size: 4949
AlwNI CAG_nnn'CTG
Cuts at: 2254 3821 2254
Size: 1567 3382
ApaLI G'TGCA_C
Cuts at: 163 3719 163
Size: 3556 1393
ApoI r'AATT_y
Cuts at: 338 1953 4302 4558 338
Size: 1615 2349 256 729
Fragments arranged by size:
2349 1615 729 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4949
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
AvaI C'yCGr_G
Cuts at: 1969 1969
Size: 4949
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1108 1141 1108
Size: 33 4916
BamHI G'GATC_C
Cuts at: 1974 1974
Size: 4949
BanI G'GyrC_C
Cuts at: 187 278 1040 1875 1965 2379 2627 2662 187
Size: 91 762 835 90 414 248 35 2474
Fragments arranged by size:
2474 835 762 414 248 91 90 35
BanII G_rGCy'C
Cuts at: 960 1963 2993 960
Size: 1003 1030 2916
Fragments arranged by size:
2916 1030 1003
BbeI G_GCGC'C
Cuts at: 1879 2383 2631 1879
Size: 504 248 4197
Fragments arranged by size:
4197 504 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1846 1880 1846
Size: 34 4915
BciVI GTATCCnnnnn_n'
Cuts at: 1109 2847 3608 4949 1109
Size: 1738 761 1341 1109
Fragments arranged by size:
1738 1341 1109 761
BclI T'GATC_A
Cuts at: 156 2469 156
Size: 2313 2636
BglI GCCn_nnn'nGGC
Cuts at: 1067 1067
Size: 4949
BglII A'GATC_T
Cuts at: 2464 4501 2464
Size: 2037 2912
BlpI GC'TnA_GC
Cuts at: 2061 2061
Size: 4949
Bme1580I G_kGCm'C
Cuts at: 167 2574 2667 3723 167
Size: 2407 93 1056 1393
Fragments arranged by size:
2407 1393 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 407 1485 2161 2242 2577 4721 407
Size: 1078 676 81 335 2144 635
Fragments arranged by size:
2144 1078 676 635 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 473 934 3316 473
Size: 461 2382 2106
Fragments arranged by size:
2382 2106 461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1884 1884
Size: 4949
Bpu10I CC'TnA_GC
Cuts at: 614 614
Size: 4949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 1375 2125 3004 3496 3794 612
Size: 763 750 879 492 298 1767
Fragments arranged by size:
1767 879 763 750 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4653 4653
Size: 4949
BsaAI yAC'GTr
Cuts at: 2302 2302
Size: 4949
BsaBI GATnn'nnATC
Cuts at: 6 1022 2463 4500 6
Size: 1016 1441 2037 455
Fragments arranged by size:
2037 1441 1016 455
BsaHI Gr'CG_yC
Cuts at: 218 1876 2380 2628 3330 4826 218
Size: 1658 504 248 702 1496 341
Fragments arranged by size:
1658 1496 702 504 341 248
BsaWI w'CCGG_w
Cuts at: 2138 2659 3337 3611 3758 4169 2138
Size: 521 678 274 147 411 2918
Fragments arranged by size:
2918 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 947 977 1906 1936 947
Size: 30 929 30 3960
Fragments arranged by size:
3960 929 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 442
Size: 4949
BseYI C'CCAG_C
Cuts at: 2374 3132 3709 4205 2374
Size: 758 577 496 3118
Fragments arranged by size:
3118 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 492
Size: 4949
BsiEI CG_ry'CG
Cuts at: 104 2491 2537 3745 104
Size: 2387 46 1208 1308
Fragments arranged by size:
2387 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 167 1963 2741 2931 3723 167
Size: 1796 778 190 792 1393
Fragments arranged by size:
1796 1393 792 778 190
BsmI GAATG_Cn'
Cuts at: 317 367 615 317
Size: 50 248 4651
Fragments arranged by size:
4651 248 50
Bsp1286I G_dGCh'C
Cuts at: 167 960 1963 2574 2667 2741 2931 2993 3723
Size: 793 1003 611 93 74 190 62 730
Cuts at: 3723 167
Size: 1393
Fragments arranged by size:
1393 1003 793 730 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4169 4169
Size: 4949
BspHI T'CATG_A
Cuts at: 794 4125 794
Size: 3331 1618
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1250 1999 2213 2515 2896 1250
Size: 749 214 302 381 3303
Fragments arranged by size:
3303 749 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3241 3295 4779 4944 50
Size: 3191 54 1484 165 55
Fragments arranged by size:
3191 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 935 2861 935
Size: 1926 3023
BsrFI r'CCGG_y
Cuts at: 1139 1843 1931 2947 3128 3337 1139
Size: 704 88 1016 181 209 2751
Fragments arranged by size:
2751 1016 704 209 181 88
BssSI C'ACGA_G
Cuts at: 3220 3578 3220
Size: 358 4591
BstAPI GCAn_nnn'nTGC
Cuts at: 780 1396 2326 780
Size: 616 930 3403
Fragments arranged by size:
3403 930 616
BstBI TT'CG_AA
Cuts at: 1269 3310 1269
Size: 2041 2908
BstYI r'GATC_y
Cuts at: 1 1974 2441 2464 2799 3045 4057 4143 4155
Size: 1973 467 23 335 246 1012 86 12
Cuts at: 4155 4501 1
Size: 346 449
Fragments arranged by size:
1973 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1376 1637 1376
Size: 261 4688
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2899 3080 3243 2899
Size: 181 163 4605
Fragments arranged by size:
4605 181 163
BtsI GCAGTG_nn'
Cuts at: 149 356 2192 149
Size: 207 1836 2906
Fragments arranged by size:
2906 1836 207
DraI TTT'AAA
Cuts at: 668 1948 668
Size: 1280 3669
DraIII CAC_nnn'GTG
Cuts at: 1314 1314
Size: 4949
DrdI GACnn_nn'nnGTC
Cuts at: 2655 3513 2655
Size: 858 4091
EaeI y'GGCC_r
Cuts at: 1841 2534 2708 3099 3126 4471 1841
Size: 693 174 391 27 1345 2319
Fragments arranged by size:
2319 1345 693 391 174 27
EagI C'GGCC_G
Cuts at: 2534 2534
Size: 4949
EarI CTCTTCn'nnn_
Cuts at: 131 2972 3182 131
Size: 2841 210 1898
Fragments arranged by size:
2841 1898 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3157 3467 3613 4759 3157
Size: 310 146 1146 3347
Fragments arranged by size:
3347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1961 1961
Size: 4949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 815 1884 2774 3206 3953 4221 350
Size: 465 1069 890 432 747 268 1078
Fragments arranged by size:
1078 1069 890 747 465 432 268
EcoO109I rG'GnC_Cy
Cuts at: 2088 2088
Size: 4949
EcoRI G'AATT_C
Cuts at: 1953 1953
Size: 4949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4949
FspI TGC'GCA
Cuts at: 2730 2730
Size: 4949
HaeII r_GCGC'y
Cuts at: 1879 2383 2631 3653 4632 1879
Size: 504 248 1022 979 2196
Fragments arranged by size:
2196 1022 979 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 967 999 1126 1158 2791 2821 2823
Size: 32 457 32 127 32 1633 30 2
Cuts at: 2823 2853 478
Size: 30 2574
Fragments arranged by size:
2574 1633 457 127 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 578 1988 578
Size: 1410 3539
HpaI GTT'AAC
Cuts at: 578 578
Size: 4949
KasI G'GCGC_C
Cuts at: 1875 2379 2627 1875
Size: 504 248 4197
Fragments arranged by size:
4197 504 248
KpnI G_GTAC'C
Cuts at: 1969 1969
Size: 4949
MfeI C'AATT_G
Cuts at: 1046 1394 1046
Size: 348 4601
MluI A'CGCG_T
Cuts at: 4382 4382
Size: 4949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1411 1937 3620 3804 1411
Size: 526 1683 184 2556
Fragments arranged by size:
2556 1683 526 184
MscI TGG'CCA
Cuts at: 2710 2710
Size: 4949
MslI CAynn'nnrTG
Cuts at: 185 226 464 598 871 1162 2938 3065 185
Size: 41 238 134 273 291 1776 127 2069
Fragments arranged by size:
2069 1776 291 273 238 134 127 41
MspA1I CmG'CkG
Cuts at: 2060 2374 2734 3747 3992 2060
Size: 314 360 1013 245 3017
Fragments arranged by size:
3017 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3130 3130
Size: 4949
NarI GG'CG_CC
Cuts at: 1876 2380 2628 1876
Size: 504 248 4197
Fragments arranged by size:
4197 504 248
NcoI C'CATG_G
Cuts at: 1376 1637 1376
Size: 261 4688
NgoMIV G'CCGG_C
Cuts at: 3128 3128
Size: 4949
NspI r_CATG'y
Cuts at: 597 918 1496 2002 2502 3409 597
Size: 321 578 506 500 907 2137
Fragments arranged by size:
2137 907 578 506 500 321
PciI A'CATG_T
Cuts at: 1492 3405 1492
Size: 1913 3036
PfoI T'CCnGG_A
Cuts at: 857 857
Size: 4949
PmeI GTTT'AAAC
Cuts at: 1948 1948
Size: 4949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4088 4120 4088
Size: 32 4917
PshAI GACnn'nnGTC
Cuts at: 840 840
Size: 4949
PstI C_TGCA'G
Cuts at: 144 363 1261 1996 2681 144
Size: 219 898 735 685 2412
Fragments arranged by size:
2412 898 735 685 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4949
PvuII CAG'CTG
Cuts at: 2374 2734 2374
Size: 360 4589
RsrII CG'GwC_CG
Cuts at: 3144 3144
Size: 4949
SacI G_AGCT'C
Cuts at: 1963 1963
Size: 4949
SalI G'TCGA_C
Cuts at: 1986 1986
Size: 4949
SapI GCTCTTCn'nnn_
Cuts at: 2972 3182 2972
Size: 210 4739
SbfI CC_TGCA'GG
Cuts at: 1996 1996
Size: 4949
ScaI AGT'ACT
Cuts at: 4466 4466
Size: 4949
SfcI C'TryA_G
Cuts at: 33 140 359 1257 1593 1992 2677 3670 3861
Size: 107 219 898 336 399 685 993 191
Cuts at: 3861 33
Size: 1121
Fragments arranged by size:
1121 993 898 685 399 336 219 191
107
SfoI GGC'GCC
Cuts at: 1877 2381 2629 1877
Size: 504 248 4197
Fragments arranged by size:
4197 504 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4949
SmaI CCC'GGG
Cuts at: 1971 1971
Size: 4949
SmlI C'TyrA_G
Cuts at: 627 1354 2104 3019 3511 3773 627
Size: 727 750 915 492 262 1803
Fragments arranged by size:
1803 915 750 727 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4949
SphI G_CATG'C
Cuts at: 918 2002 2502 918
Size: 1084 500 3365
Fragments arranged by size:
3365 1084 500
SspI AAT'ATT
Cuts at: 286 286
Size: 4949
StuI AGG'CCT
Cuts at: 828 828
Size: 4949
StyI C'CwwG_G
Cuts at: 1376 1637 2083 2437 1376
Size: 261 446 354 3888
Fragments arranged by size:
3888 446 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1058 1145 2532 2665 3124 3333 1058
Size: 87 1387 133 459 209 2674
Fragments arranged by size:
2674 1387 459 209 133 87
TatI w'GTAC_w
Cuts at: 4464 4464
Size: 4949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4466 4498 4466
Size: 32 4917
Tth111I GACn'n_nGTC
Cuts at: 2746 2746
Size: 4949
XbaI T'CTAG_A
Cuts at: 1980 1980
Size: 4949
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1507 2444 1507
Size: 937 4012
XmaI C'CCGG_G
Cuts at: 1969 1969
Size: 4949
ZraI GAC'GTC
Cuts at: 219 219
Size: 4949
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BbsI BbvCI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI