Restriction map for
pF1KA1825
(Circular) (Six-base) MAPSORT of: pF1KA1825.seq Check: 5214 from: 1 to: 6725
pF1KA1825 6725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 502 2570 2601 502
Size: 2068 31 4626
Fragments arranged by size:
4626 2068 31
AccI GT'mk_AC
Cuts at: 1016 1148 3317 3416 3763 1016
Size: 132 2169 99 347 3978
Fragments arranged by size:
3978 2169 347 132 99
Acc65I G'GTAC_C
Cuts at: 1504 3741 1504
Size: 2237 4488
AclI AA'CG_TT
Cuts at: 6565 6565
Size: 6725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2017 2683 2743 4550 4982 5729 5997 1336
Size: 681 666 60 1807 432 747 268 2064
Fragments arranged by size:
2064 1807 747 681 666 432 268 60
AfeI AGC'GCT
Cuts at: 1975 1975
Size: 6725
AflIII A'CryG_T
Cuts at: 968 1643 4077 5181 6158 968
Size: 675 2434 1104 977 1535
Fragments arranged by size:
2434 1535 1104 977 675
AgeI A'CCGG_T
Cuts at: 5113 5113
Size: 6725
AleI CACnn'nnGTG
Cuts at: 1273 1861 3049 1273
Size: 588 1188 4949
Fragments arranged by size:
4949 1188 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 154 403 1402 2497 2500 154
Size: 249 999 1095 3 4379
Fragments arranged by size:
4379 1095 999 249 3
AlwNI CAG_nnn'CTG
Cuts at: 358 1959 2388 2586 2919 3171 3636 4030 5597
Size: 1601 429 198 333 252 465 394 1567
Cuts at: 5597 358
Size: 1486
Fragments arranged by size:
1601 1567 1486 465 429 394 333 252
198
ApaI G_GGCC'C
Cuts at: 153 1818 2076 153
Size: 1665 258 4802
Fragments arranged by size:
4802 1665 258
ApaLI G'TGCA_C
Cuts at: 472 1548 3298 5495 472
Size: 1076 1750 2197 1702
Fragments arranged by size:
2197 1750 1702 1076
ApoI r'AATT_y
Cuts at: 625 769 1964 3729 6078 6334 625
Size: 144 1195 1765 2349 256 1016
Fragments arranged by size:
2349 1765 1195 1016 256 144
AseI AT'TA_AT
Cuts at: 20 20
Size: 6725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6725
AvaI C'yCGr_G
Cuts at: 190 456 2144 2210 2495 2795 2929 3230 3349
Size: 266 1688 66 285 300 134 301 119
Cuts at: 3349 3493 3745 190
Size: 144 252 3170
Fragments arranged by size:
3170 1688 301 300 285 266 252 144
134 119 66
BamHI G'GATC_C
Cuts at: 1667 3750 1667
Size: 2083 4642
BanI G'GyrC_C
Cuts at: 138 296 346 540 798 878 1107 1267 1504
Size: 158 50 194 258 80 229 160 237
Cuts at: 1504 1855 2701 2716 3741 4155 4403 4438 138
Size: 351 846 15 1025 414 248 35 2425
Fragments arranged by size:
2425 1025 846 414 351 258 248 237
229 194 160 158 80 50 35 15
BanII G_rGCy'C
Cuts at: 153 572 875 1196 1661 1818 2038 2076 2157
Size: 419 303 321 465 157 220 38 81
Cuts at: 2157 2503 2867 3349 3739 4769 153
Size: 346 364 482 390 1030 2109
Fragments arranged by size:
2109 1030 482 465 419 390 364 346
321 303 220 157 81 38
BbeI G_GCGC'C
Cuts at: 300 2720 4159 4407 300
Size: 2420 1439 248 2618
Fragments arranged by size:
2618 2420 1439 248
BbsI GAAGACnn'nnnn_
Cuts at: 605 746 1208 605
Size: 141 462 6122
Fragments arranged by size:
6122 462 141
BbvCI CC'TCA_GC
Cuts at: 1207 1207
Size: 6725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1121 1155 1121
Size: 34 6691
BciVI GTATCCnnnnn_n'
Cuts at: 4623 5384 6725 4623
Size: 761 1341 4623
Fragments arranged by size:
4623 1341 761
BclI T'GATC_A
Cuts at: 2342 3026 4245 2342
Size: 684 1219 4822
Fragments arranged by size:
4822 1219 684
BglI GCCn_nnn'nGGC
Cuts at: 112 177 226 276 2543 2626 3587 112
Size: 65 49 50 2267 83 961 3250
Fragments arranged by size:
3250 2267 961 83 65 50 49
BglII A'GATC_T
Cuts at: 2138 4240 6277 2138
Size: 2102 2037 2586
Fragments arranged by size:
2586 2102 2037
BlpI GC'TnA_GC
Cuts at: 3837 3837
Size: 6725
Bme1580I G_kGCm'C
Cuts at: 143 153 476 1270 1552 1818 1995 2076 2110
Size: 10 323 794 282 266 177 81 34
Cuts at: 2110 3302 4350 4443 5499 143
Size: 1192 1048 93 1056 1369
Fragments arranged by size:
1369 1192 1056 1048 794 323 282 266
177 93 81 34 10
BmgBI CAC'GTC
Cuts at: 1119 1254 2712 2736 1119
Size: 135 1458 24 5108
Fragments arranged by size:
5108 1458 135 24
BmrI ACTGGGnnnn_n'
Cuts at: 1777 2097 2968 3937 4018 4353 6497 1777
Size: 320 871 969 81 335 2144 2005
Fragments arranged by size:
2144 2005 969 871 335 320 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2478 5092 2478
Size: 2614 4111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1216 1453 1564 2254 2542 2823 3148 3271
Size: 537 237 111 690 288 281 325 123
Cuts at: 3271 679
Size: 4133
Fragments arranged by size:
4133 690 537 325 288 281 237 123
111
Bpu10I CC'TnA_GC
Cuts at: 1207 1580 2765 2812 2908 3307 1207
Size: 373 1185 47 96 399 4625
Fragments arranged by size:
4625 1185 399 373 96 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 2230 2266 3202 3901 4780 5272 5570 628
Size: 1602 36 936 699 879 492 298 1783
Fragments arranged by size:
1783 1602 936 879 699 492 298 36
BsaI GGTCTCn'nnnn_
Cuts at: 1429 2274 2918 6429 1429
Size: 845 644 3511 1725
Fragments arranged by size:
3511 1725 845 644
BsaAI yAC'GTr
Cuts at: 2376 2679 3024 4078 2376
Size: 303 345 1054 5023
Fragments arranged by size:
5023 1054 345 303
BsaBI GATnn'nnATC
Cuts at: 6 4239 6276 6
Size: 4233 2037 455
Fragments arranged by size:
4233 2037 455
BsaHI Gr'CG_yC
Cuts at: 297 2717 4156 4404 5106 6602 297
Size: 2420 1439 248 702 1496 420
Fragments arranged by size:
2420 1496 1439 702 420 248
BsaWI w'CCGG_w
Cuts at: 980 1357 1664 3914 4435 5113 5387 5534 5945
Size: 377 307 2250 521 678 274 147 411
Cuts at: 5945 980
Size: 1760
Fragments arranged by size:
2250 1760 678 521 411 377 307 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 1060 1090 2144 2174 544
Size: 30 486 30 1054 30 5095
Fragments arranged by size:
5095 1054 486 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2893 3544 3552 2893
Size: 651 8 6066
Fragments arranged by size:
6066 651 8
BseYI C'CCAG_C
Cuts at: 365 371 514 1516 2031 2169 2184 2579 2670
Size: 6 143 1002 515 138 15 395 91
Cuts at: 2670 4150 4908 5485 5981 365
Size: 1480 758 577 496 1109
Fragments arranged by size:
1480 1109 1002 758 577 515 496 395
143 138 91 15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 480 565 970 1234 1300 1635 2071 2259
Size: 71 85 405 264 66 335 436 188
Cuts at: 2259 2374 2629 3027 409
Size: 115 255 398 4107
Fragments arranged by size:
4107 436 405 398 335 264 255 188
115 85 71 66
BsiEI CG_ry'CG
Cuts at: 104 1086 1571 1898 2063 2860 3672 4267 4313
Size: 982 485 327 165 797 812 595 46
Cuts at: 4313 5521 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 982 812 797 595 485 327
165 46
BsiHKAI G_wGCw'C
Cuts at: 476 572 1552 3302 3739 4517 4707 5499 476
Size: 96 980 1750 437 778 190 792 1702
Fragments arranged by size:
1750 1702 980 792 778 437 190 96
BsmI GAATG_Cn'
Cuts at: 2325 2325
Size: 6725
BsmBI CGTCTCn'nnnn_
Cuts at: 1080 1757 1080
Size: 677 6048
Bsp1286I G_dGCh'C
Cuts at: 143 153 476 572 875 1196 1270 1552 1661
Size: 10 323 96 303 321 74 282 109
Cuts at: 1661 1818 1995 2038 2076 2110 2157 2503 2867
Size: 157 177 43 38 34 47 346 364
Cuts at: 2867 3302 3349 3739 4350 4443 4517 4707 4769
Size: 435 47 390 611 93 74 190 62
Cuts at: 4769 5499 143
Size: 730 1369
Fragments arranged by size:
1369 730 611 435 390 364 346 323
321 303 282 190 177 157 109 96
93 74 74 62 47 47 43 38
34 10
BspEI T'CCGG_A
Cuts at: 980 1664 5945 980
Size: 684 4281 1760
Fragments arranged by size:
4281 1760 684
BspHI T'CATG_A
Cuts at: 2339 5901 2339
Size: 3562 3163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 502 2570 2601 3775 3989 4291 4672 502
Size: 2068 31 1174 214 302 381 2555
Fragments arranged by size:
2555 2068 1174 381 302 214 31
BsrBI CCG'CTC
Cuts at: 50 1088 2280 2361 2769 2781 5017 5071 6555
Size: 1038 1192 81 408 12 2236 54 1484
Cuts at: 6555 6720 50
Size: 165 55
Fragments arranged by size:
2236 1484 1192 1038 408 165 81 55
54 12
BsrDI GCAATG_nn'
Cuts at: 480 594 1040 3623 4637 480
Size: 114 446 2583 1014 2568
Fragments arranged by size:
2583 2568 1014 446 114
BsrFI r'CCGG_y
Cuts at: 225 275 1320 2444 3181 4723 4904 5113 225
Size: 50 1045 1124 737 1542 181 209 1837
Fragments arranged by size:
1837 1542 1124 1045 737 209 181 50
BsrGI T'GTAC_A
Cuts at: 3263 3377 3263
Size: 114 6611
BssHII G'CGCG_C
Cuts at: 204 206 485 204
Size: 2 279 6444
Fragments arranged by size:
6444 279 2
BssSI C'ACGA_G
Cuts at: 412 571 1824 1861 3592 4996 5354 412
Size: 159 1253 37 1731 1404 358 1783
Fragments arranged by size:
1783 1731 1404 1253 358 159 37
BstAPI GCAn_nnn'nTGC
Cuts at: 960 4102 960
Size: 3142 3583
BstBI TT'CG_AA
Cuts at: 623 5086 623
Size: 4463 2262
BstEII G'GTnAC_C
Cuts at: 1776 1867 3051 1776
Size: 91 1184 5450
Fragments arranged by size:
5450 1184 91
BstXI CCAn_nnnn'nTGG
Cuts at: 927 2712 3441 927
Size: 1785 729 4211
Fragments arranged by size:
4211 1785 729
BstYI r'GATC_y
Cuts at: 1 392 749 977 1667 2138 2312 3074 3750
Size: 391 357 228 690 471 174 762 676
Cuts at: 3750 4217 4240 4575 4821 5833 5919 5931 6277
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6277 1
Size: 449
Fragments arranged by size:
1012 762 690 676 471 467 449 391
357 346 335 246 228 174 86 23
12
BtgI C'CryG_G
Cuts at: 107 563 680 1307 1898 3428 107
Size: 456 117 627 591 1530 3404
Fragments arranged by size:
3404 1530 627 591 456 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1458 1608 2040 2958 4675 4856 5019 1458
Size: 150 432 918 1717 181 163 3164
Fragments arranged by size:
3164 1717 918 432 181 163 150
BtsI GCAGTG_nn'
Cuts at: 2363 2455 3968 2363
Size: 92 1513 5120
Fragments arranged by size:
5120 1513 92
DraI TTT'AAA
Cuts at: 3724 3724
Size: 6725
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 6725
DrdI GACnn_nn'nnGTC
Cuts at: 1785 4431 5289 1785
Size: 2646 858 3221
Fragments arranged by size:
3221 2646 858
EaeI y'GGCC_r
Cuts at: 268 1083 1604 1631 1751 1895 2060 2753 3179
Size: 815 521 27 120 144 165 693 426
Cuts at: 3179 3425 3431 3443 3536 3665 4310 4484 4875
Size: 246 6 12 93 129 645 174 391
Cuts at: 4875 4902 6247 268
Size: 27 1345 746
Fragments arranged by size:
1345 815 746 693 645 521 426 391
246 174 165 144 129 120 93 27
27 12 6
EagI C'GGCC_G
Cuts at: 1083 1895 2060 4310 1083
Size: 812 165 2250 3498
Fragments arranged by size:
3498 2250 812 165
EarI CTCTTCn'nnn_
Cuts at: 1405 1459 3199 4748 4958 1405
Size: 54 1740 1549 210 3172
Fragments arranged by size:
3172 1740 1549 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2581 4933 5243 5389 6535 2581
Size: 2352 310 146 1146 2771
Fragments arranged by size:
2771 2352 1146 310 146
EcoICRI GAG'CTC
Cuts at: 570 3737 570
Size: 3167 3558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1216 1336 1453 1564 2017 2254 2542 2683
Size: 537 120 117 111 453 237 288 141
Cuts at: 2683 2743 2823 3148 3271 4550 4982 5729 5997
Size: 60 80 325 123 1279 432 747 268
Cuts at: 5997 679
Size: 1407
Fragments arranged by size:
1407 1279 747 537 453 432 325 288
268 237 141 123 120 117 111 80
60
EcoNI CCTnn'n_nnAGG
Cuts at: 2895 2911 2895
Size: 16 6709
EcoO109I rG'GnC_Cy
Cuts at: 149 1512 1815 2072 2073 2229 3633 3695 3864
Size: 1363 303 257 1 156 1404 62 169
Cuts at: 3864 149
Size: 3010
Fragments arranged by size:
3010 1404 1363 303 257 169 156 62
1
EcoRI G'AATT_C
Cuts at: 625 3729 625
Size: 3104 3621
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 622 4744 622
Size: 4122 2603
FspI TGC'GCA
Cuts at: 1390 4506 1390
Size: 3116 3609
HaeII r_GCGC'y
Cuts at: 232 289 300 1977 2170 2463 2720 2749 3085
Size: 57 11 1677 193 293 257 29 336
Cuts at: 3085 3661 4159 4407 5429 6408 232
Size: 576 498 248 1022 979 549
Fragments arranged by size:
1677 1022 979 576 549 498 336 293
257 248 193 57 29 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 601 633 1060 1092 2144 2176 4567 4597 4599
Size: 32 427 32 1052 32 2391 30 2
Cuts at: 4599 4629 601
Size: 30 2697
Fragments arranged by size:
2697 2391 1052 427 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1326 1611 1721 3405 3764 1326
Size: 285 110 1684 359 4287
Fragments arranged by size:
4287 1684 359 285 110
KasI G'GCGC_C
Cuts at: 296 2716 4155 4403 296
Size: 2420 1439 248 2618
Fragments arranged by size:
2618 2420 1439 248
KpnI G_GTAC'C
Cuts at: 1508 3745 1508
Size: 2237 4488
MluI A'CGCG_T
Cuts at: 6158 6158
Size: 6725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 953 5396 5580 953
Size: 4443 184 2098
Fragments arranged by size:
4443 2098 184
MscI TGG'CCA
Cuts at: 1633 2755 3427 3433 3445 3538 4486 1633
Size: 1122 672 6 12 93 948 3872
Fragments arranged by size:
3872 1122 948 672 93 12 6
MslI CAynn'nnrTG
Cuts at: 587 925 1273 1861 3049 3280 3427 3433 3439
Size: 338 348 588 1188 231 147 6 6
Cuts at: 3439 3604 4714 4841 587
Size: 165 1110 127 2471
Fragments arranged by size:
2471 1188 1110 588 348 338 231 165
147 127 6 6
MspA1I CmG'CkG
Cuts at: 118 358 760 1139 1331 1483 1845 2586 2856
Size: 240 402 379 192 152 362 741 270
Cuts at: 2856 3041 3836 4150 4510 5523 5768 118
Size: 185 795 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 795 741 402 379 362 360
314 270 245 240 192 185 152
NaeI GCC'GGC
Cuts at: 227 2446 3183 4906 227
Size: 2219 737 1723 2046
Fragments arranged by size:
2219 2046 1723 737
NarI GG'CG_CC
Cuts at: 297 2717 4156 4404 297
Size: 2420 1439 248 2618
Fragments arranged by size:
2618 2420 1439 248
NcoI C'CATG_G
Cuts at: 107 3428 107
Size: 3321 3404
NgoMIV G'CCGG_C
Cuts at: 225 2444 3181 4904 225
Size: 2219 737 1723 2046
Fragments arranged by size:
2219 2046 1723 737
NspI r_CATG'y
Cuts at: 972 1647 2372 2731 3778 4278 5185 972
Size: 675 725 359 1047 500 907 2512
Fragments arranged by size:
2512 1047 907 725 675 500 359
PasI CC'CwG_GG
Cuts at: 1374 1374
Size: 6725
PciI A'CATG_T
Cuts at: 968 1643 5181 968
Size: 675 3538 2512
Fragments arranged by size:
3538 2512 675
PflMI CCAn_nnn'nTGG
Cuts at: 2334 2334
Size: 6725
PfoI T'CCnGG_A
Cuts at: 734 2306 2839 3641 734
Size: 1572 533 802 3818
Fragments arranged by size:
3818 1572 802 533
PmeI GTTT'AAAC
Cuts at: 3724 3724
Size: 6725
PmlI CAC'GTG
Cuts at: 2376 3024 2376
Size: 648 6077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5864 5896 5864
Size: 32 6693
PpuMI rG'GwC_Cy
Cuts at: 1512 3633 3695 1512
Size: 2121 62 4542
Fragments arranged by size:
4542 2121 62
PspOMI G'GGCC_C
Cuts at: 149 1814 2072 149
Size: 1665 258 4802
Fragments arranged by size:
4802 1665 258
PspXI vC'TCGA_Gb
Cuts at: 2929 2929
Size: 6725
PstI C_TGCA'G
Cuts at: 1482 2426 2567 3683 3772 4457 1482
Size: 944 141 1116 89 685 3750
Fragments arranged by size:
3750 1116 944 685 141 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6725
PvuII CAG'CTG
Cuts at: 760 1483 1845 2586 4150 4510 760
Size: 723 362 741 1564 360 2975
Fragments arranged by size:
2975 1564 741 723 362 360
RsrII CG'GwC_CG
Cuts at: 1355 4920 1355
Size: 3565 3160
SacI G_AGCT'C
Cuts at: 572 3739 572
Size: 3167 3558
SalI G'TCGA_C
Cuts at: 3762 3762
Size: 6725
SanDI GG'GwC_CC
Cuts at: 3695 3695
Size: 6725
SapI GCTCTTCn'nnn_
Cuts at: 4748 4958 4748
Size: 210 6515
SbfI CC_TGCA'GG
Cuts at: 2567 3772 2567
Size: 1205 5520
ScaI AGT'ACT
Cuts at: 892 6242 892
Size: 5350 1375
SexAI A'CCwGG_T
Cuts at: 1103 1103
Size: 6725
SfcI C'TryA_G
Cuts at: 33 900 1478 1799 2422 2563 3061 3105 3163
Size: 867 578 321 623 141 498 44 58
Cuts at: 3163 3679 3768 4453 5446 5637 33
Size: 516 89 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 623 578 516 498
321 191 141 89 58 44
SfoI GGC'GCC
Cuts at: 298 2718 4157 4405 298
Size: 2420 1439 248 2618
Fragments arranged by size:
2618 2420 1439 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6725
SmaI CCC'GGG
Cuts at: 192 2146 2212 2497 2797 3747 192
Size: 1954 66 285 300 950 3170
Fragments arranged by size:
3170 1954 950 300 285 66
SmlI C'TyrA_G
Cuts at: 607 2245 2281 2929 3217 3880 4795 5287 5549
Size: 1638 36 648 288 663 915 492 262
Cuts at: 5549 607
Size: 1783
Fragments arranged by size:
1783 1638 915 663 648 492 288 262
36
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6725
SphI G_CATG'C
Cuts at: 2372 2731 3778 4278 2372
Size: 359 1047 500 4819
Fragments arranged by size:
4819 1047 500 359
SrfI GCCC'GGGC
Cuts at: 192 192
Size: 6725
StuI AGG'CCT
Cuts at: 940 940
Size: 6725
StyI C'CwwG_G
Cuts at: 107 1271 1508 2294 3428 3859 4213 107
Size: 1164 237 786 1134 431 354 2619
Fragments arranged by size:
2619 1164 1134 786 431 354 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 375 1557 1855 2728 4308 4441 4900 5109
Size: 41 1182 298 873 1580 133 459 209
Cuts at: 5109 334
Size: 1950
Fragments arranged by size:
1950 1580 1182 873 459 298 209 133
41
TatI w'GTAC_w
Cuts at: 890 1646 3263 3377 6240 890
Size: 756 1617 114 2863 1375
Fragments arranged by size:
2863 1617 1375 756 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6242 6274 6242
Size: 32 6693
Tth111I GACn'n_nGTC
Cuts at: 1072 3673 4522 1072
Size: 2601 849 3275
Fragments arranged by size:
3275 2601 849
XbaI T'CTAG_A
Cuts at: 1875 3756 1875
Size: 1881 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 570 1731 2955 4220 570
Size: 1161 1224 1265 3075
Fragments arranged by size:
3075 1265 1224 1161
XhoI C'TCGA_G
Cuts at: 2929 2929
Size: 6725
XmaI C'CCGG_G
Cuts at: 190 2144 2210 2495 2795 3745 190
Size: 1954 66 285 300 950 3170
Fragments arranged by size:
3170 1954 950 300 285 66
XmnI GAAnn'nnTTC
Cuts at: 1930 1930
Size: 6725
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PsrI SacII SfiI SgrAI
SnaBI SspI SwaI ZraI