Restriction map for
pF1KA1816
(Circular) (Six-base) MAPSORT of: pF1KA1816.seq Check: 7856 from: 1 to: 6512
pF1KA1816 6512 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 3550 3550
Size: 6512
Acc65I G'GTAC_C
Cuts at: 389 3528 389
Size: 3139 3373
AclI AA'CG_TT
Cuts at: 6352 6352
Size: 6512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 4337 4769 5122 5784 731
Size: 3606 432 353 662 1459
Fragments arranged by size:
3606 1459 662 432 353
AflIII A'CryG_T
Cuts at: 1209 1490 3365 3864 5664 5945 1209
Size: 281 1875 499 1800 281 1776
Fragments arranged by size:
1875 1800 1776 499 281 281
AleI CACnn'nnGTG
Cuts at: 325 325
Size: 6512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 659 659
Size: 6512
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 798 830 798
Size: 32 6480
AlwNI CAG_nnn'CTG
Cuts at: 1089 1144 1468 2370 3108 3817 5255 1089
Size: 55 324 902 738 709 1438 2346
Fragments arranged by size:
2346 1438 902 738 709 324 55
ApaI G_GGCC'C
Cuts at: 286 2340 286
Size: 2054 4458
ApaLI G'TGCA_C
Cuts at: 5350 5350
Size: 6512
ApoI r'AATT_y
Cuts at: 657 680 1126 3516 5865 6121 657
Size: 23 446 2390 2349 256 1048
Fragments arranged by size:
2390 2349 1048 446 256 23
AseI AT'TA_AT
Cuts at: 20 1013 1042 20
Size: 993 29 5490
Fragments arranged by size:
5490 993 29
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6512
AvaI C'yCGr_G
Cuts at: 510 3195 3532 510
Size: 2685 337 3490
Fragments arranged by size:
3490 2685 337
AvrII C'CTAG_G
Cuts at: 2914 2914
Size: 6512
BamHI G'GATC_C
Cuts at: 3537 3537
Size: 6512
BanI G'GyrC_C
Cuts at: 365 389 484 490 3046 3208 3528 3942 4190
Size: 24 95 6 2556 162 320 414 248
Cuts at: 4190 4225 365
Size: 35 2652
Fragments arranged by size:
2652 2556 414 320 248 162 95 35
24 6
BanII G_rGCy'C
Cuts at: 286 612 2340 3526 4556 286
Size: 326 1728 1186 1030 2242
Fragments arranged by size:
2242 1728 1186 1030 326
BbeI G_GCGC'C
Cuts at: 488 3050 3946 4194 488
Size: 2562 896 248 2806
Fragments arranged by size:
2806 2562 896 248
BbsI GAAGACnn'nnnn_
Cuts at: 1106 1789 2096 1106
Size: 683 307 5522
Fragments arranged by size:
5522 683 307
BciVI GTATCCnnnnn_n'
Cuts at: 4410 5466 6512 4410
Size: 1056 1046 4410
Fragments arranged by size:
4410 1056 1046
BclI T'GATC_A
Cuts at: 569 1023 1826 2328 4032 569
Size: 454 803 502 1704 3049
Fragments arranged by size:
3049 1704 803 502 454
BglI GCCn_nnn'nGGC
Cuts at: 290 2074 290
Size: 1784 4728
BglII A'GATC_T
Cuts at: 831 4027 6064 831
Size: 3196 2037 1279
Fragments arranged by size:
3196 2037 1279
BlpI GC'TnA_GC
Cuts at: 3624 3624
Size: 6512
Bme1580I G_kGCm'C
Cuts at: 286 1481 2079 2231 2340 3142 4137 4230 5354
Size: 1195 598 152 109 802 995 93 1124
Cuts at: 5354 286
Size: 1444
Fragments arranged by size:
1444 1195 1124 995 802 598 152 109
93
BmgBI CAC'GTC
Cuts at: 372 3366 372
Size: 2994 3518
BmrI ACTGGGnnnn_n'
Cuts at: 722 2334 2476 3299 3724 3805 4140 6284 722
Size: 1612 142 823 425 81 335 2144 950
Fragments arranged by size:
2144 1612 950 823 425 335 142 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 558 4879 558
Size: 4321 2191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 442 481 913 1370 1481 2047 2443 2536
Size: 6 39 432 457 111 566 396 93
Cuts at: 2536 436
Size: 4412
Fragments arranged by size:
4412 566 457 432 396 111 93 39
6
Bpu10I CC'TnA_GC
Cuts at: 401 2083 2238 3244 401
Size: 1682 155 1006 3669
Fragments arranged by size:
3669 1682 1006 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4567 5281 5579 3688
Size: 879 714 298 4621
Fragments arranged by size:
4621 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 623 1141 1655 2256 6216 623
Size: 518 514 601 3960 919
Fragments arranged by size:
3960 919 601 518 514
BsaAI yAC'GTr
Cuts at: 3865 3865
Size: 6512
BsaBI GATnn'nnATC
Cuts at: 6 4026 6063 6
Size: 4020 2037 455
Fragments arranged by size:
4020 2037 455
BsaHI Gr'CG_yC
Cuts at: 485 3047 3943 4191 4893 6389 485
Size: 2562 896 248 702 1496 608
Fragments arranged by size:
2562 1496 896 702 608 248
BsaWI w'CCGG_w
Cuts at: 3701 4222 4900 5311 5458 5732 3701
Size: 521 678 411 147 274 4481
Fragments arranged by size:
4481 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 798 828 3003 3033 798
Size: 30 2175 30 4277
Fragments arranged by size:
4277 2175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 746 3003 746
Size: 2257 4255
BseYI C'CCAG_C
Cuts at: 521 1480 2870 3067 3159 3285 3394 3937 4695
Size: 959 1390 197 92 126 109 543 758
Cuts at: 4695 5360 5768 521
Size: 665 408 1265
Fragments arranged by size:
1390 1265 959 758 665 543 408 197
126 109 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1508 2138 2506 3446 1508
Size: 630 368 940 4574
Fragments arranged by size:
4574 940 630 368
BsiEI CG_ry'CG
Cuts at: 104 305 4054 4100 5330 104
Size: 201 3749 46 1230 1286
Fragments arranged by size:
3749 1286 1230 201 46
BsiHKAI G_wGCw'C
Cuts at: 612 2719 2973 3526 4304 4494 5354 612
Size: 2107 254 553 778 190 860 1770
Fragments arranged by size:
2107 1770 860 778 553 254 190
BsmI GAATG_Cn'
Cuts at: 3357 3357
Size: 6512
Bsp1286I G_dGCh'C
Cuts at: 286 612 1481 2079 2231 2340 2719 2973 3142
Size: 326 869 598 152 109 379 254 169
Cuts at: 3142 3526 4137 4230 4304 4494 4556 5354 286
Size: 384 611 93 74 190 62 798 1444
Fragments arranged by size:
1444 869 798 611 598 384 379 326
254 190 169 152 109 93 74 62
BspHI T'CATG_A
Cuts at: 2111 2309 4944 2111
Size: 198 2635 3679
Fragments arranged by size:
3679 2635 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1784 2481 2808 3562 3776 4078 4459 1784
Size: 697 327 754 214 302 381 3837
Fragments arranged by size:
3837 754 697 381 327 302 214
BsrBI CCG'CTC
Cuts at: 50 333 3348 4804 4858 6342 6507 50
Size: 283 3015 1456 54 1484 165 55
Fragments arranged by size:
3015 1484 1456 283 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4424 4424
Size: 6512
BsrFI r'CCGG_y
Cuts at: 259 487 493 1450 4510 4691 259
Size: 228 6 957 3060 181 2080
Fragments arranged by size:
3060 2080 957 228 181 6
BsrGI T'GTAC_A
Cuts at: 2705 2705
Size: 6512
BssSI C'ACGA_G
Cuts at: 4783 5491 4783
Size: 708 5804
BstAPI GCAn_nnn'nTGC
Cuts at: 1345 3003 3183 3889 1345
Size: 1658 180 706 3968
Fragments arranged by size:
3968 1658 706 180
BstBI TT'CG_AA
Cuts at: 684 4873 684
Size: 4189 2323
BstEII G'GTnAC_C
Cuts at: 3499 3499
Size: 6512
BstXI CCAn_nnnn'nTGG
Cuts at: 640 2623 640
Size: 1983 4529
BstYI r'GATC_y
Cuts at: 1 831 1222 3537 4004 4027 4362 4608 4914
Size: 830 391 2315 467 23 335 246 306
Cuts at: 4914 4926 5012 6064 1
Size: 12 86 1052 449
Fragments arranged by size:
2315 1052 830 467 449 391 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1070 1656 3359 1070
Size: 586 1703 4223
Fragments arranged by size:
4223 1703 586
BtgI C'CryG_G
Cuts at: 107 323 1217 1274 2141 2166 3167 107
Size: 216 894 57 867 25 1001 3452
Fragments arranged by size:
3452 1001 894 867 216 57 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3173 4462 4643 4806 3173
Size: 1289 181 163 4879
Fragments arranged by size:
4879 1289 181 163
BtsI GCAGTG_nn'
Cuts at: 739 891 1445 3755 739
Size: 152 554 2310 3496
Fragments arranged by size:
3496 2310 554 152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3363 3398 3363
Size: 35 6477
DraI TTT'AAA
Cuts at: 3511 3511
Size: 6512
DrdI GACnn_nn'nnGTC
Cuts at: 4218 5562 4218
Size: 1344 5168
EaeI y'GGCC_r
Cuts at: 302 1055 1277 1448 1955 2633 4097 4271 4662
Size: 753 222 171 507 678 1464 174 391
Cuts at: 4662 4689 6034 302
Size: 27 1345 780
Fragments arranged by size:
1464 1345 780 753 678 507 391 222
174 171 27
EagI C'GGCC_G
Cuts at: 302 4097 302
Size: 3795 2717
EarI CTCTTCn'nnn_
Cuts at: 764 1103 1174 3065 4535 4745 764
Size: 339 71 1891 1470 210 2531
Fragments arranged by size:
2531 1891 1470 339 210 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 328 562 2431 4720 5462 5608 6322 328
Size: 234 1869 2289 742 146 714 518
Fragments arranged by size:
2289 1869 742 714 518 234 146
EcoICRI GAG'CTC
Cuts at: 610 3524 610
Size: 2914 3598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 442 481 731 913 1370 1481 2047 2443
Size: 6 39 250 182 457 111 566 396
Cuts at: 2443 2536 4337 4769 5122 5784 436
Size: 93 1801 432 353 662 1164
Fragments arranged by size:
1801 1164 662 566 457 432 396 353
250 182 111 93 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2913 3247 2913
Size: 334 6178
EcoO109I rG'GnC_Cy
Cuts at: 282 283 1086 1465 1660 2067 2209 2272 2608
Size: 1 803 379 195 407 142 63 336
Cuts at: 2608 2918 3149 3651 282
Size: 310 231 502 3143
Fragments arranged by size:
3143 803 502 407 379 336 310 231
195 142 63 1
EcoRI G'AATT_C
Cuts at: 680 1126 3516 680
Size: 446 2390 3676
Fragments arranged by size:
3676 2390 446
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3111 4531 3111
Size: 1420 5092
FspI TGC'GCA
Cuts at: 2648 4293 2648
Size: 1645 4867
HaeII r_GCGC'y
Cuts at: 429 488 3050 3946 4194 5424 6195 429
Size: 59 2562 896 248 1230 771 746
Fragments arranged by size:
2562 1230 896 771 746 248 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 4354 4384 4386 4416 231
Size: 32 4091 30 2 30 2327
Fragments arranged by size:
4091 2327 32 30 30 2
HincII GTy'rAC
Cuts at: 375 876 3551 375
Size: 501 2675 3336
Fragments arranged by size:
3336 2675 501
KasI G'GCGC_C
Cuts at: 484 3046 3942 4190 484
Size: 2562 896 248 2806
Fragments arranged by size:
2806 2562 896 248
KpnI G_GTAC'C
Cuts at: 393 3532 393
Size: 3139 3373
MluI A'CGCG_T
Cuts at: 5945 5945
Size: 6512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 581 691 2169 2633 2799 5271 5455 581
Size: 110 1478 464 166 2472 184 1638
Fragments arranged by size:
2472 1638 1478 464 184 166 110
MscI TGG'CCA
Cuts at: 1057 1957 2635 4273 1057
Size: 900 678 1638 3296
Fragments arranged by size:
3296 1638 900 678
MslI CAynn'nnrTG
Cuts at: 325 1822 1919 2722 2851 4501 4628 325
Size: 1497 97 803 129 1650 127 2209
Fragments arranged by size:
2209 1650 1497 803 129 127 97
MspA1I CmG'CkG
Cuts at: 130 232 414 453 2334 2469 2644 3163 3623
Size: 102 182 39 1881 135 175 519 460
Cuts at: 3623 3937 4297 5081 5326 130
Size: 314 360 784 245 1316
Fragments arranged by size:
1881 1316 784 519 460 360 314 245
182 175 135 102 39
NaeI GCC'GGC
Cuts at: 489 4693 489
Size: 4204 2308
NarI GG'CG_CC
Cuts at: 485 3047 3943 4191 485
Size: 2562 896 248 2806
Fragments arranged by size:
2806 2562 896 248
NcoI C'CATG_G
Cuts at: 107 1217 2141 3167 107
Size: 1110 924 1026 3452
Fragments arranged by size:
3452 1110 1026 924
NdeI CA'TA_TG
Cuts at: 1677 1677
Size: 6512
NgoMIV G'CCGG_C
Cuts at: 487 4691 487
Size: 4204 2308
NspI r_CATG'y
Cuts at: 738 1213 1494 2562 2850 3006 3267 3565 4065
Size: 475 281 1068 288 156 261 298 500
Cuts at: 4065 5668 738
Size: 1603 1582
Fragments arranged by size:
1603 1582 1068 500 475 298 288 281
261 156
PasI CC'CwG_GG
Cuts at: 2072 2623 2784 2072
Size: 551 161 5800
Fragments arranged by size:
5800 551 161
PciI A'CATG_T
Cuts at: 1209 1490 5664 1209
Size: 281 4174 2057
Fragments arranged by size:
4174 2057 281
PflMI CCAn_nnn'nTGG
Cuts at: 625 625
Size: 6512
PfoI T'CCnGG_A
Cuts at: 3416 3416
Size: 6512
PmeI GTTT'AAAC
Cuts at: 3511 3511
Size: 6512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 798 830 4958 4990 798
Size: 32 4128 32 2320
Fragments arranged by size:
4128 2320 32 32
PpuMI rG'GwC_Cy
Cuts at: 1086 1660 2608 3149 1086
Size: 574 948 541 4449
Fragments arranged by size:
4449 948 574 541
PshAI GACnn'nnGTC
Cuts at: 1931 1931
Size: 6512
PspOMI G'GGCC_C
Cuts at: 282 2336 282
Size: 2054 4458
PstI C_TGCA'G
Cuts at: 1698 1781 2942 3559 4244 1698
Size: 83 1161 617 685 3966
Fragments arranged by size:
3966 1161 685 617 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6512
PvuII CAG'CTG
Cuts at: 414 2644 3163 3937 4297 414
Size: 2230 519 774 360 2629
Fragments arranged by size:
2629 2230 774 519 360
RsrII CG'GwC_CG
Cuts at: 4707 4707
Size: 6512
SacI G_AGCT'C
Cuts at: 612 3526 612
Size: 2914 3598
SalI G'TCGA_C
Cuts at: 3549 3549
Size: 6512
SapI GCTCTTCn'nnn_
Cuts at: 1174 4535 4745 1174
Size: 3361 210 2941
Fragments arranged by size:
3361 2941 210
SbfI CC_TGCA'GG
Cuts at: 3559 3559
Size: 6512
ScaI AGT'ACT
Cuts at: 225 6029 225
Size: 5804 708
SfcI C'TryA_G
Cuts at: 33 1694 1751 1777 2938 3555 4240 5208 5399
Size: 1661 57 26 1161 617 685 968 191
Cuts at: 5399 33
Size: 1146
Fragments arranged by size:
1661 1161 1146 968 685 617 191 57
26
SfoI GGC'GCC
Cuts at: 486 3048 3944 4192 486
Size: 2562 896 248 2806
Fragments arranged by size:
2806 2562 896 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6512
SmaI CCC'GGG
Cuts at: 3197 3534 3197
Size: 337 6175
SmlI C'TyrA_G
Cuts at: 3667 4582 5296 5558 3667
Size: 915 714 262 4621
Fragments arranged by size:
4621 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1282 3016 67
Size: 1215 1734 3563
Fragments arranged by size:
3563 1734 1215
SphI G_CATG'C
Cuts at: 2562 2850 3006 3267 3565 4065 2562
Size: 288 156 261 298 500 5009
Fragments arranged by size:
5009 500 298 288 261 156
SspI AAT'ATT
Cuts at: 1881 1881
Size: 6512
StuI AGG'CCT
Cuts at: 1239 2908 1239
Size: 1669 4843
StyI C'CwwG_G
Cuts at: 107 1217 2141 2914 2932 3167 3646 4000 107
Size: 1110 924 773 18 235 479 354 2619
Fragments arranged by size:
2619 1110 924 773 479 354 235 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2805 3299 4095 4228 4687 4896 2805
Size: 494 796 133 459 209 4421
Fragments arranged by size:
4421 796 494 459 209 133
TatI w'GTAC_w
Cuts at: 223 2705 6027 223
Size: 2482 3322 708
Fragments arranged by size:
3322 2482 708
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3003 3035 6029 6061 3003
Size: 32 2994 32 3454
Fragments arranged by size:
3454 2994 32 32
Tth111I GACn'n_nGTC
Cuts at: 4309 4309
Size: 6512
XbaI T'CTAG_A
Cuts at: 825 3543 825
Size: 2718 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3467 4007 3467
Size: 540 5972
XmaI C'CCGG_G
Cuts at: 3195 3532 3195
Size: 337 6175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AscI BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmBI BspEI BssHII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI