Restriction map for
pF1KA1796
(Circular) (Six-base) MAPSORT of: pF1KA1796.seq Check: 5526 from: 1 to: 4241
pF1KA1796 4241 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 1177 1279 1177
Size: 102 4139
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 647 780 2066 2498 2851 3513 647
Size: 133 1286 432 353 662 1375
Fragments arranged by size:
1375 1286 662 432 353 133
AflII C'TTAA_G
Cuts at: 649 649
Size: 4241
AflIII A'CryG_T
Cuts at: 292 1233 1593 3393 3674 292
Size: 941 360 1800 281 859
Fragments arranged by size:
1800 941 859 360 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 373
Size: 32 4209
AlwNI CAG_nnn'CTG
Cuts at: 155 1546 2984 155
Size: 1391 1438 1412
Fragments arranged by size:
1438 1412 1391
ApaLI G'TGCA_C
Cuts at: 3079 3079
Size: 4241
ApoI r'AATT_y
Cuts at: 397 571 811 988 1017 1033 1245 3594 3850
Size: 174 240 177 29 16 212 2349 256
Cuts at: 3850 397
Size: 788
Fragments arranged by size:
2349 788 256 240 212 177 174 29
16
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 114 1135 1257 1671 1919 1954 114
Size: 1021 122 414 248 35 2401
Fragments arranged by size:
2401 1021 414 248 122 35
BanII G_rGCy'C
Cuts at: 1255 2285 1255
Size: 1030 3211
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbvCI CC'TCA_GC
Cuts at: 178 178
Size: 4241
BciVI GTATCCnnnnn_n'
Cuts at: 2139 3195 4241 2139
Size: 1056 1046 2139
Fragments arranged by size:
2139 1056 1046
BclI T'GATC_A
Cuts at: 167 1761 167
Size: 1594 2647
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 1353 1353
Size: 4241
Bme1580I G_kGCm'C
Cuts at: 1138 1866 1959 3083 1138
Size: 728 93 1124 2296
Fragments arranged by size:
2296 1124 728 93
BmgBI CAC'GTC
Cuts at: 1234 1234
Size: 4241
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 4241
Bpu10I CC'TnA_GC
Cuts at: 178 178
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 3010 3308 1417
Size: 879 714 298 2350
Fragments arranged by size:
2350 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3945 3945
Size: 4241
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 3040 3187 3461 1430
Size: 521 678 411 147 274 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 373
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4241
BseYI C'CCAG_C
Cuts at: 1201 1666 2424 3089 3497 1201
Size: 465 758 665 408 1945
Fragments arranged by size:
1945 758 665 465 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 561 529
Size: 32 4209
BsiEI CG_ry'CG
Cuts at: 104 207 1783 1829 3059 104
Size: 103 1576 46 1230 1286
Fragments arranged by size:
1576 1286 1230 103 46
BsiHKAI G_wGCw'C
Cuts at: 1255 2033 2223 3083 1255
Size: 778 190 860 2413
Fragments arranged by size:
2413 860 778 190
BsmI GAATG_Cn'
Cuts at: 723 723
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 1138 1255 1866 1959 2033 2223 2285 3083 1138
Size: 117 611 93 74 190 62 798 2296
Fragments arranged by size:
2296 798 611 190 117 93 74 62
BspHI T'CATG_A
Cuts at: 1166 2673 1166
Size: 1507 2734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 2239 2420 2239
Size: 181 4060
BsrGI T'GTAC_A
Cuts at: 929 1045 929
Size: 116 4125
BssSI C'ACGA_G
Cuts at: 2512 3220 2512
Size: 708 3533
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 426 426
Size: 4241
BstYI r'GATC_y
Cuts at: 1 1122 1197 1266 1733 1756 2091 2337 2643
Size: 1121 75 69 467 23 335 246 306
Cuts at: 2643 2655 2741 3793 1
Size: 12 86 1052 449
Fragments arranged by size:
1121 1052 467 449 335 306 246 86
75 69 23 12
BtgI C'CryG_G
Cuts at: 107 462 756 1139 107
Size: 355 294 383 3209
Fragments arranged by size:
3209 383 355 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 512 932 1484 512
Size: 420 552 3269
Fragments arranged by size:
3269 552 420
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 410 445 410
Size: 35 4206
DraI TTT'AAA
Cuts at: 402 1240 402
Size: 838 3403
DrdI GACnn_nn'nnGTC
Cuts at: 1947 3291 1947
Size: 1344 2897
EaeI y'GGCC_r
Cuts at: 1826 2000 2391 2418 3763 1826
Size: 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 3191 3337 4051 2449
Size: 742 146 714 2639
Fragments arranged by size:
2639 742 714 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 647 780 2066 2498 2851 3513 205
Size: 442 133 1286 432 353 662 933
Fragments arranged by size:
1286 933 662 442 432 353 133
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 571 811 1245 571
Size: 240 434 3567
Fragments arranged by size:
3567 434 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1005 1675 1923 3153 3924 1005
Size: 670 248 1230 771 1322
Fragments arranged by size:
1322 1230 771 670 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 316 348 2083 2113 2115 2145 316
Size: 32 1735 30 2 30 2412
Fragments arranged by size:
2412 1735 32 30 30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MfeI C'AATT_G
Cuts at: 610 610
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 596 1209 3000 3184 596
Size: 613 1791 184 1653
Fragments arranged by size:
1791 1653 613 184
MscI TGG'CCA
Cuts at: 2002 2002
Size: 4241
MslI CAynn'nnrTG
Cuts at: 112 410 716 2230 2357 112
Size: 298 306 1514 127 1996
Fragments arranged by size:
1996 1514 306 298 127
MspA1I CmG'CkG
Cuts at: 198 800 1205 1352 1666 2026 2810 3055 198
Size: 602 405 147 314 360 784 245 1384
Fragments arranged by size:
1384 784 602 405 360 314 245 147
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 107 756 107
Size: 649 3592
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 648 1294 1794 3397 648
Size: 646 500 1603 1492
Fragments arranged by size:
1603 1492 646 500
PciI A'CATG_T
Cuts at: 3393 3393
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 373 405 2687 2719 373
Size: 32 2282 32 1895
Fragments arranged by size:
2282 1895 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 245 277 480 512 245
Size: 32 203 32 3974
Fragments arranged by size:
3974 203 32 32
PstI C_TGCA'G
Cuts at: 805 1288 1973 805
Size: 483 685 3073
Fragments arranged by size:
3073 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 198 800 1205 1666 2026 198
Size: 602 405 461 360 2413
Fragments arranged by size:
2413 602 461 405 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 801 1284 1969 2937 3128 33
Size: 768 483 685 968 191 1146
Fragments arranged by size:
1146 968 768 685 483 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 649 1396 2311 3025 3287 649
Size: 747 915 714 262 1603
Fragments arranged by size:
1603 915 747 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 648 1294 1794 648
Size: 646 500 3095
Fragments arranged by size:
3095 646 500
SspI AAT'ATT
Cuts at: 688 688
Size: 4241
StyI C'CwwG_G
Cuts at: 107 756 1375 1729 107
Size: 649 619 354 2619
Fragments arranged by size:
2619 649 619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1824 1957 2416 2625 1824
Size: 133 459 209 3440
Fragments arranged by size:
3440 459 209 133
TatI w'GTAC_w
Cuts at: 929 1045 3756 929
Size: 116 2711 1414
Fragments arranged by size:
2711 1414 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 1736 426
Size: 1310 2931
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
XmnI GAAnn'nnTTC
Cuts at: 254 254
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI