Restriction map for
pF1KA1789
(Circular) (Six-base) MAPSORT of: pF1KA1789.seq Check: 8424 from: 1 to: 4514
pF1KA1789 4514 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1160 2339 2771 3124 3786 1145
Size: 15 1179 432 353 662 1873
Fragments arranged by size:
1873 1179 662 432 353 15
AflII C'TTAA_G
Cuts at: 1372 1372
Size: 4514
AflIII A'CryG_T
Cuts at: 1866 3666 3947 1866
Size: 1800 281 2433
Fragments arranged by size:
2433 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 392 424 392
Size: 32 4482
AlwNI CAG_nnn'CTG
Cuts at: 1064 1819 3257 1064
Size: 755 1438 2321
Fragments arranged by size:
2321 1438 755
ApaLI G'TGCA_C
Cuts at: 3352 3352
Size: 4514
ApoI r'AATT_y
Cuts at: 228 1518 3867 4123 228
Size: 1290 2349 256 619
Fragments arranged by size:
2349 1290 619 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 1534 1534
Size: 4514
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 1530 1944 2192 2227 1530
Size: 414 248 35 3817
Fragments arranged by size:
3817 414 248 35
BanII G_rGCy'C
Cuts at: 1528 2558 1528
Size: 1030 3484
BbeI G_GCGC'C
Cuts at: 1948 2196 1948
Size: 248 4266
BbsI GAAGACnn'nnnn_
Cuts at: 244 496 244
Size: 252 4262
BciVI GTATCCnnnnn_n'
Cuts at: 591 2412 3468 4514 591
Size: 1821 1056 1046 591
Fragments arranged by size:
1821 1056 1046 591
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 1626 1626
Size: 4514
Bme1580I G_kGCm'C
Cuts at: 2139 2232 3356 2139
Size: 93 1124 3297
Fragments arranged by size:
3297 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1726 1807 2142 4286 1726
Size: 81 335 2144 1954
Fragments arranged by size:
2144 1954 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1110 1690 2569 3283 3581 1110
Size: 580 879 714 298 2043
Fragments arranged by size:
2043 879 714 580 298
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 1945 2193 2895 4391 1945
Size: 248 702 1496 2068
Fragments arranged by size:
2068 1496 702 248
BsaWI w'CCGG_w
Cuts at: 164 425 1703 2224 2902 3313 3460 3734 164
Size: 261 1278 521 678 411 147 274 944
Fragments arranged by size:
1278 944 678 521 411 274 261 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 392 422 392
Size: 30 4484
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1354 1354
Size: 4514
BseYI C'CCAG_C
Cuts at: 1939 2697 3362 3770 1939
Size: 758 665 408 2683
Fragments arranged by size:
2683 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 304 820 287
Size: 17 516 3981
Fragments arranged by size:
3981 516 17
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3332 104
Size: 1952 46 1230 1286
Fragments arranged by size:
1952 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1528 2306 2496 3356 1528
Size: 778 190 860 2686
Fragments arranged by size:
2686 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1528 2139 2232 2306 2496 2558 3356 1528
Size: 611 93 74 190 62 798 2686
Fragments arranged by size:
2686 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 425 425
Size: 4514
BspHI T'CATG_A
Cuts at: 1011 2946 1011
Size: 1935 2579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1564 1778 2080 2461 1180
Size: 384 214 302 381 3233
Fragments arranged by size:
3233 384 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 467 996 2426 467
Size: 529 1430 2555
Fragments arranged by size:
2555 1430 529
BsrFI r'CCGG_y
Cuts at: 2512 2693 2512
Size: 181 4333
BssSI C'ACGA_G
Cuts at: 2785 3493 2785
Size: 708 3806
BstAPI GCAn_nnn'nTGC
Cuts at: 1891 1891
Size: 4514
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstYI r'GATC_y
Cuts at: 1 555 1539 2006 2029 2364 2610 2916 2928
Size: 554 984 467 23 335 246 306 12
Cuts at: 2928 3014 4066 1
Size: 86 1052 449
Fragments arranged by size:
1052 984 554 467 449 335 306 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 1757 1757
Size: 4514
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3564 2220
Size: 1344 3170
EaeI y'GGCC_r
Cuts at: 2099 2273 2664 2691 4036 2099
Size: 174 391 27 1345 2577
Fragments arranged by size:
2577 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 326 1164 1207 1466 2537 2747 326
Size: 838 43 259 1071 210 2093
Fragments arranged by size:
2093 1071 838 259 210 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2722 3464 3610 4324 2722
Size: 742 146 714 2912
Fragments arranged by size:
2912 742 714 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1160 2339 2771 3124 3786 1145
Size: 15 1179 432 353 662 1873
Fragments arranged by size:
1873 1179 662 432 353 15
EcoO109I rG'GnC_Cy
Cuts at: 1653 1653
Size: 4514
EcoRI G'AATT_C
Cuts at: 228 1518 228
Size: 1290 3224
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 532 796 2533 532
Size: 264 1737 2513
Fragments arranged by size:
2513 1737 264
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 1948 2196 3426 4197 1948
Size: 248 1230 771 2265
Fragments arranged by size:
2265 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 353 385 1083 1115 1340 1372 1393 1425 2356
Size: 32 698 32 225 32 21 32 931
Cuts at: 2356 2386 2388 2418 353
Size: 30 2 30 2449
Fragments arranged by size:
2449 931 698 225 32 32 32 32
30 30 21 2
HincII GTy'rAC
Cuts at: 1051 1553 1051
Size: 502 4012
HindIII A'AGCT_T
Cuts at: 1369 1369
Size: 4514
KasI G'GCGC_C
Cuts at: 1944 2192 1944
Size: 248 4266
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 893 3273 3457 893
Size: 2380 184 1950
Fragments arranged by size:
2380 1950 184
MscI TGG'CCA
Cuts at: 2275 2275
Size: 4514
MslI CAynn'nnrTG
Cuts at: 280 796 2503 2630 280
Size: 516 1707 127 2164
Fragments arranged by size:
2164 1707 516 127
MspA1I CmG'CkG
Cuts at: 1625 1939 2299 3083 3328 1625
Size: 314 360 784 245 2811
Fragments arranged by size:
2811 784 360 314 245
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 1945 2193 1945
Size: 248 4266
NdeI CA'TA_TG
Cuts at: 495 759 495
Size: 264 4250
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NspI r_CATG'y
Cuts at: 177 1567 2067 3670 177
Size: 1390 500 1603 1021
Fragments arranged by size:
1603 1390 1021 500
PciI A'CATG_T
Cuts at: 3666 3666
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 392 424 2960 2992 392
Size: 32 2536 32 1914
Fragments arranged by size:
2536 1914 32 32
PstI C_TGCA'G
Cuts at: 1561 2246 1561
Size: 685 3829
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 1939 2299 1939
Size: 360 4154
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SfcI C'TryA_G
Cuts at: 33 857 1557 2242 3210 3401 33
Size: 824 700 685 968 191 1146
Fragments arranged by size:
1146 968 824 700 685 191
SfoI GGC'GCC
Cuts at: 1946 2194 1946
Size: 248 4266
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 1089 1372 1669 2584 3298 3560 1089
Size: 283 297 915 714 262 2043
Fragments arranged by size:
2043 915 714 297 283 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1567 2067 1567
Size: 500 4014
SspI AAT'ATT
Cuts at: 450 450
Size: 4514
StyI C'CwwG_G
Cuts at: 1648 2002 1648
Size: 354 4160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 534 4029 534
Size: 3495 1019
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4482
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2009 2009
Size: 4514
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI