Restriction map for
pF1KA1788
(Circular) (Six-base) MAPSORT of: pF1KA1788.seq Check: 7149 from: 1 to: 4346
pF1KA1788 4346 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AccI GT'mk_AC
Cuts at: 391 1384 391
Size: 993 3353
Acc65I G'GTAC_C
Cuts at: 1362 1362
Size: 4346
AclI AA'CG_TT
Cuts at: 4186 4186
Size: 4346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2603 3350 3618 2171
Size: 432 747 268 2899
Fragments arranged by size:
2899 747 432 268
AflIII A'CryG_T
Cuts at: 827 1698 2802 3779 827
Size: 871 1104 977 1394
Fragments arranged by size:
1394 1104 977 871
AgeI A'CCGG_T
Cuts at: 2734 2734
Size: 4346
AlwNI CAG_nnn'CTG
Cuts at: 324 1651 3218 324
Size: 1327 1567 1452
Fragments arranged by size:
1567 1452 1327
ApaI G_GGCC'C
Cuts at: 300 677 300
Size: 377 3969
ApaLI G'TGCA_C
Cuts at: 3116 3116
Size: 4346
ApoI r'AATT_y
Cuts at: 1350 3699 3955 1350
Size: 2349 256 1741
Fragments arranged by size:
2349 1741 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4346
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
AvaI C'yCGr_G
Cuts at: 992 1278 1366 992
Size: 286 88 3972
Fragments arranged by size:
3972 286 88
BamHI G'GATC_C
Cuts at: 1371 1371
Size: 4346
BanI G'GyrC_C
Cuts at: 487 1089 1362 1776 2024 2059 487
Size: 602 273 414 248 35 2774
Fragments arranged by size:
2774 602 414 273 248 35
BanII G_rGCy'C
Cuts at: 199 300 364 586 677 1253 1360 2390 199
Size: 101 64 222 91 576 107 1030 2155
Fragments arranged by size:
2155 1030 576 222 107 101 91 64
BbeI G_GCGC'C
Cuts at: 491 1780 2028 491
Size: 1289 248 2809
Fragments arranged by size:
2809 1289 248
BbsI GAAGACnn'nnnn_
Cuts at: 795 817 795
Size: 22 4324
BbvCI CC'TCA_GC
Cuts at: 1225 1225
Size: 4346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 473 507 931 965 473
Size: 34 424 34 3854
Fragments arranged by size:
3854 424 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2244 3005 4346 2244
Size: 761 1341 2244
Fragments arranged by size:
2244 1341 761
BclI T'GATC_A
Cuts at: 1866 1866
Size: 4346
BglI GCCn_nnn'nGGC
Cuts at: 1241 1241
Size: 4346
BglII A'GATC_T
Cuts at: 1861 3898 1861
Size: 2037 2309
BlpI GC'TnA_GC
Cuts at: 1458 1458
Size: 4346
Bme1580I G_kGCm'C
Cuts at: 300 677 1971 2064 3120 300
Size: 377 1294 93 1056 1526
Fragments arranged by size:
1526 1294 1056 377 93
BmgBI CAC'GTC
Cuts at: 828 828
Size: 4346
BmrI ACTGGGnnnn_n'
Cuts at: 1558 1639 1974 4118 1558
Size: 81 335 2144 1786
Fragments arranged by size:
2144 1786 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 508 805 814 358
Size: 150 297 9 3890
Fragments arranged by size:
3890 297 150 9
Bpu10I CC'TnA_GC
Cuts at: 1225 1225
Size: 4346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2401 2893 3191 1522
Size: 879 492 298 2677
Fragments arranged by size:
2677 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4050 4050
Size: 4346
BsaAI yAC'GTr
Cuts at: 1182 1699 1182
Size: 517 3829
BsaBI GATnn'nnATC
Cuts at: 6 1860 3897 6
Size: 1854 2037 455
Fragments arranged by size:
2037 1854 455
BsaHI Gr'CG_yC
Cuts at: 161 284 488 499 1777 2025 2727 4223 161
Size: 123 204 11 1278 248 702 1496 284
Fragments arranged by size:
1496 1278 702 284 248 204 123 11
BsaWI w'CCGG_w
Cuts at: 758 1535 2056 2734 3008 3155 3566 758
Size: 777 521 678 274 147 411 1538
Fragments arranged by size:
1538 777 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 184
Size: 30 4316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 802 802
Size: 4346
BseYI C'CCAG_C
Cuts at: 310 349 383 404 449 661 703 1771 2529
Size: 39 34 21 45 212 42 1068 758
Cuts at: 2529 3106 3602 310
Size: 577 496 1054
Fragments arranged by size:
1068 1054 758 577 496 212 45 42
39 34 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 901
Size: 4346
BsiEI CG_ry'CG
Cuts at: 104 149 257 1888 1934 3142 104
Size: 45 108 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 108 46 45
BsiHKAI G_wGCw'C
Cuts at: 586 1253 1316 1360 2138 2328 3120 586
Size: 667 63 44 778 190 792 1812
Fragments arranged by size:
1812 792 778 667 190 63 44
BsmI GAATG_Cn'
Cuts at: 117 117
Size: 4346
BsmBI CGTCTCn'nnnn_
Cuts at: 132 132
Size: 4346
Bsp1286I G_dGCh'C
Cuts at: 199 300 364 586 677 1253 1316 1360 1971
Size: 101 64 222 91 576 63 44 611
Cuts at: 1971 2064 2138 2328 2390 3120 199
Size: 93 74 190 62 730 1425
Fragments arranged by size:
1425 730 611 576 222 190 101 93
91 74 64 63 62 44
BspEI T'CCGG_A
Cuts at: 3566 3566
Size: 4346
BspHI T'CATG_A
Cuts at: 3522 3522
Size: 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 549 873 936 1396 1610 1912 2293 549
Size: 324 63 460 214 302 381 2602
Fragments arranged by size:
2602 460 381 324 302 214 63
BsrBI CCG'CTC
Cuts at: 50 2638 2692 4176 4341 50
Size: 2588 54 1484 165 55
Fragments arranged by size:
2588 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2258 2258
Size: 4346
BsrFI r'CCGG_y
Cuts at: 144 2344 2525 2734 144
Size: 2200 181 209 1756
Fragments arranged by size:
2200 1756 209 181
BssSI C'ACGA_G
Cuts at: 2617 2975 2617
Size: 358 3988
BstAPI GCAn_nnn'nTGC
Cuts at: 1723 1723
Size: 4346
BstBI TT'CG_AA
Cuts at: 951 2707 951
Size: 1756 2590
BstXI CCAn_nnnn'nTGG
Cuts at: 711 1123 711
Size: 412 3934
BstYI r'GATC_y
Cuts at: 1 1371 1838 1861 2196 2442 3454 3540 3552
Size: 1370 467 23 335 246 1012 86 12
Cuts at: 3552 3898 1
Size: 346 449
Fragments arranged by size:
1370 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 155 155
Size: 4346
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1269 2296 2477 2640 1269
Size: 1027 181 163 2975
Fragments arranged by size:
2975 1027 181 163
BtsI GCAGTG_nn'
Cuts at: 965 1589 965
Size: 624 3722
DraI TTT'AAA
Cuts at: 1345 1345
Size: 4346
DrdI GACnn_nn'nnGTC
Cuts at: 2052 2910 2052
Size: 858 3488
EaeI y'GGCC_r
Cuts at: 146 254 540 848 1322 1931 2105 2496 2523
Size: 108 286 308 474 609 174 391 27
Cuts at: 2523 3868 146
Size: 1345 624
Fragments arranged by size:
1345 624 609 474 391 308 286 174
108 27
EagI C'GGCC_G
Cuts at: 146 254 1931 146
Size: 108 1677 2561
Fragments arranged by size:
2561 1677 108
EarI CTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 768 2554 2864 3010 4156 768
Size: 1786 310 146 1146 958
Fragments arranged by size:
1786 1146 958 310 146
EcoICRI GAG'CTC
Cuts at: 584 1251 1358 584
Size: 667 107 3572
Fragments arranged by size:
3572 667 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 508 805 814 2171 2603 3350 3618 358
Size: 150 297 9 1357 432 747 268 1086
Fragments arranged by size:
1357 1086 747 432 297 268 150 9
EcoNI CCTnn'n_nnAGG
Cuts at: 868 868
Size: 4346
EcoO109I rG'GnC_Cy
Cuts at: 321 673 674 1485 321
Size: 352 1 811 3182
Fragments arranged by size:
3182 811 352 1
EcoRI G'AATT_C
Cuts at: 1350 1350
Size: 4346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 4346
FspI TGC'GCA
Cuts at: 2127 2127
Size: 4346
HaeII r_GCGC'y
Cuts at: 311 491 1780 2028 3050 4029 311
Size: 180 1289 248 1022 979 628
Fragments arranged by size:
1289 1022 979 628 248 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 2188 2218 2220 2250 184
Size: 32 1972 30 2 30 2280
Fragments arranged by size:
2280 1972 32 30 30 2
HincII GTy'rAC
Cuts at: 392 1385 392
Size: 993 3353
KasI G'GCGC_C
Cuts at: 487 1776 2024 487
Size: 1289 248 2809
Fragments arranged by size:
2809 1289 248
KpnI G_GTAC'C
Cuts at: 1366 1366
Size: 4346
MluI A'CGCG_T
Cuts at: 3779 3779
Size: 4346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3017 3201 3017
Size: 184 4162
MscI TGG'CCA
Cuts at: 850 2107 850
Size: 1257 3089
MslI CAynn'nnrTG
Cuts at: 2335 2462 2335
Size: 127 4219
MspA1I CmG'CkG
Cuts at: 151 539 783 846 1457 1771 2131 3144 3389
Size: 388 244 63 611 314 360 1013 245
Cuts at: 3389 151
Size: 1108
Fragments arranged by size:
1108 1013 611 388 360 314 245 244
63
NaeI GCC'GGC
Cuts at: 146 2527 146
Size: 2381 1965
NarI GG'CG_CC
Cuts at: 488 1777 2025 488
Size: 1289 248 2809
Fragments arranged by size:
2809 1289 248
NcoI C'CATG_G
Cuts at: 155 155
Size: 4346
NdeI CA'TA_TG
Cuts at: 972 972
Size: 4346
NgoMIV G'CCGG_C
Cuts at: 144 2525 144
Size: 2381 1965
NspI r_CATG'y
Cuts at: 1104 1399 1899 2806 1104
Size: 295 500 907 2644
Fragments arranged by size:
2644 907 500 295
PasI CC'CwG_GG
Cuts at: 588 588
Size: 4346
PciI A'CATG_T
Cuts at: 2802 2802
Size: 4346
PflMI CCAn_nnn'nTGG
Cuts at: 1331 1331
Size: 4346
PfoI T'CCnGG_A
Cuts at: 219 524 219
Size: 305 4041
PmeI GTTT'AAAC
Cuts at: 1345 1345
Size: 4346
PmlI CAC'GTG
Cuts at: 1182 1182
Size: 4346
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 4314
PpuMI rG'GwC_Cy
Cuts at: 321 321
Size: 4346
PshAI GACnn'nnGTC
Cuts at: 124 124
Size: 4346
PspOMI G'GGCC_C
Cuts at: 296 673 296
Size: 377 3969
PspXI vC'TCGA_Gb
Cuts at: 1278 1278
Size: 4346
PstI C_TGCA'G
Cuts at: 1393 2078 1393
Size: 685 3661
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4346
PvuII CAG'CTG
Cuts at: 783 846 1771 2131 783
Size: 63 925 360 2998
Fragments arranged by size:
2998 925 360 63
RsrII CG'GwC_CG
Cuts at: 1266 2541 1266
Size: 1275 3071
SacI G_AGCT'C
Cuts at: 586 1253 1360 586
Size: 667 107 3572
Fragments arranged by size:
3572 667 107
SalI G'TCGA_C
Cuts at: 390 1383 390
Size: 993 3353
SapI GCTCTTCn'nnn_
Cuts at: 2369 2579 2369
Size: 210 4136
SbfI CC_TGCA'GG
Cuts at: 1393 1393
Size: 4346
ScaI AGT'ACT
Cuts at: 3863 3863
Size: 4346
SfcI C'TryA_G
Cuts at: 33 168 1389 2074 3067 3258 33
Size: 135 1221 685 993 191 1121
Fragments arranged by size:
1221 1121 993 685 191 135
SfoI GGC'GCC
Cuts at: 489 1778 2026 489
Size: 1289 248 2809
Fragments arranged by size:
2809 1289 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4346
SmaI CCC'GGG
Cuts at: 1368 1368
Size: 4346
SmlI C'TyrA_G
Cuts at: 1278 1501 2416 2908 3170 1278
Size: 223 915 492 262 2454
Fragments arranged by size:
2454 915 492 262 223
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4346
SphI G_CATG'C
Cuts at: 1399 1899 1399
Size: 500 3846
StuI AGG'CCT
Cuts at: 1235 1235
Size: 4346
StyI C'CwwG_G
Cuts at: 155 1307 1480 1834 155
Size: 1152 173 354 2667
Fragments arranged by size:
2667 1152 354 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 1929 2062 2521 2730 580
Size: 1349 133 459 209 2196
Fragments arranged by size:
2196 1349 459 209 133
TatI w'GTAC_w
Cuts at: 3861 3861
Size: 4346
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4314
Tth111I GACn'n_nGTC
Cuts at: 2143 2143
Size: 4346
XbaI T'CTAG_A
Cuts at: 1377 1377
Size: 4346
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 780 1841 780
Size: 1061 3285
XhoI C'TCGA_G
Cuts at: 1278 1278
Size: 4346
XmaI C'CCGG_G
Cuts at: 1366 1366
Size: 4346
XmnI GAAnn'nnTTC
Cuts at: 802 802
Size: 4346
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI