Restriction map for
pF1KA1782
(Circular) (Six-base) MAPSORT of: pF1KA1782.seq Check: 5684 from: 1 to: 4727
pF1KA1782 4727 bp
With 182 enzymes: SgfI *
June 20, 2008 10:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1219 1219
Size: 4727
AatII G_ACGT'C
Cuts at: 455 455
Size: 4727
AccI GT'mk_AC
Cuts at: 1172 1200 1765 1172
Size: 28 565 4134
Fragments arranged by size:
4134 565 28
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 966 1611 2552 2984 3337 3999 824
Size: 142 645 941 432 353 662 1552
Fragments arranged by size:
1552 941 662 645 432 353 142
AflIII A'CryG_T
Cuts at: 2079 3879 4160 2079
Size: 1800 281 2646
Fragments arranged by size:
2646 1800 281
AgeI A'CCGG_T
Cuts at: 1673 1673
Size: 4727
AleI CACnn'nnGTG
Cuts at: 512 1420 512
Size: 908 3819
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 442 1457 442
Size: 1015 3712
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 160 192 160
Size: 32 4695
AlwNI CAG_nnn'CTG
Cuts at: 2032 3470 2032
Size: 1438 3289
ApaI G_GGCC'C
Cuts at: 189 200 189
Size: 11 4716
ApaLI G'TGCA_C
Cuts at: 493 3565 493
Size: 3072 1655
ApoI r'AATT_y
Cuts at: 1681 1731 4080 4336 1681
Size: 50 2349 256 2072
Fragments arranged by size:
2349 2072 256 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 181 1014 1220 1280 1747 181
Size: 833 206 60 467 3161
Fragments arranged by size:
3161 833 467 206 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1557 1590 1557
Size: 33 4694
BamHI G'GATC_C
Cuts at: 1216 1752 1216
Size: 536 4191
BanI G'GyrC_C
Cuts at: 544 773 858 1489 1743 2157 2405 2440 544
Size: 229 85 631 254 414 248 35 2831
Fragments arranged by size:
2831 631 414 254 248 229 85 35
BanII G_rGCy'C
Cuts at: 189 200 371 1741 2771 189
Size: 11 171 1370 1030 2145
Fragments arranged by size:
2145 1370 1030 171 11
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbsI GAAGACnn'nnnn_
Cuts at: 125 125
Size: 4727
BciVI GTATCCnnnnn_n'
Cuts at: 1389 1585 2625 3681 4727 1389
Size: 196 1040 1056 1046 1389
Fragments arranged by size:
1389 1056 1046 1040 196
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1839 1839
Size: 4727
Bme1580I G_kGCm'C
Cuts at: 189 200 497 1492 2352 2445 3569 189
Size: 11 297 995 860 93 1124 1347
Fragments arranged by size:
1347 1124 995 860 297 93 11
BmgBI CAC'GTC
Cuts at: 1593 1593
Size: 4727
BmrI ACTGGGnnnn_n'
Cuts at: 184 1939 2020 2355 4499 184
Size: 1755 81 335 2144 412
Fragments arranged by size:
2144 1755 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 423 1282 3094 423
Size: 859 1812 2056
Fragments arranged by size:
2056 1812 859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 775 871 1225 187
Size: 588 96 354 3689
Fragments arranged by size:
3689 588 354 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1903 2782 3496 3794 427
Size: 1476 879 714 298 1360
Fragments arranged by size:
1476 1360 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1625 4431 1625
Size: 2806 1921
BsaAI yAC'GTr
Cuts at: 844 2080 844
Size: 1236 3491
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 452 2158 2406 3108 4604 452
Size: 1706 248 702 1496 575
Fragments arranged by size:
1706 1496 702 575 248
BsaWI w'CCGG_w
Cuts at: 1673 1916 2437 3115 3526 3673 3947 1673
Size: 243 521 678 411 147 274 2453
Fragments arranged by size:
2453 678 521 411 274 243 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 160 190 667 697 160
Size: 30 477 30 4190
Fragments arranged by size:
4190 477 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 301 675 772 1258 301
Size: 374 97 486 3770
Fragments arranged by size:
3770 486 374 97
BseYI C'CCAG_C
Cuts at: 303 1312 1403 2152 2910 3575 3983 303
Size: 1009 91 749 758 665 408 1047
Fragments arranged by size:
1047 1009 758 749 665 408 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 859 1118 574
Size: 285 259 4183
Fragments arranged by size:
4183 285 259
BsiEI CG_ry'CG
Cuts at: 104 909 2269 2315 3545 104
Size: 805 1360 46 1230 1286
Fragments arranged by size:
1360 1286 1230 805 46
BsiHKAI G_wGCw'C
Cuts at: 211 488 497 764 1568 1741 2519 2709 3569
Size: 277 9 267 804 173 778 190 860
Cuts at: 3569 211
Size: 1369
Fragments arranged by size:
1369 860 804 778 277 267 190 173
9
Bsp1286I G_dGCh'C
Cuts at: 189 200 211 371 488 497 764 1492 1568
Size: 11 11 160 117 9 267 728 76
Cuts at: 1568 1741 2352 2445 2519 2709 2771 3569 189
Size: 173 611 93 74 190 62 798 1347
Fragments arranged by size:
1347 798 728 611 267 190 173 160
117 93 76 74 62 11 11 9
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 1219 1777 1991 2293 2674 578
Size: 641 558 214 302 381 2631
Fragments arranged by size:
2631 641 558 381 302 214
BsrBI CCG'CTC
Cuts at: 50 134 242 318 771 891 3019 3073 4557
Size: 84 108 76 453 120 2128 54 1484
Cuts at: 4557 4722 50
Size: 165 55
Fragments arranged by size:
2128 1484 453 165 120 108 84 76
55 54
BsrDI GCAATG_nn'
Cuts at: 132 528 1323 2639 132
Size: 396 795 1316 2220
Fragments arranged by size:
2220 1316 795 396
BsrFI r'CCGG_y
Cuts at: 641 1274 1430 1673 2725 2906 641
Size: 633 156 243 1052 181 2462
Fragments arranged by size:
2462 1052 633 243 181 156
BsrGI T'GTAC_A
Cuts at: 275 275
Size: 4727
BssSI C'ACGA_G
Cuts at: 2998 3706 2998
Size: 708 4019
BstAPI GCAn_nnn'nTGC
Cuts at: 1322 2104 1322
Size: 782 3945
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstEII G'GTnAC_C
Cuts at: 661 661
Size: 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 267 1422 1425 267
Size: 1155 3 3569
Fragments arranged by size:
3569 1155 3
BstYI r'GATC_y
Cuts at: 1 1216 1752 2219 2242 2577 2823 3129 3141
Size: 1215 536 467 23 335 246 306 12
Cuts at: 3141 3227 4279 1
Size: 86 1052 449
Fragments arranged by size:
1215 1052 536 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1241 1388 1241
Size: 147 4580
BtgI C'CryG_G
Cuts at: 107 1620 107
Size: 1513 3214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 296 2677 2858 3021 296
Size: 2381 181 163 2002
Fragments arranged by size:
2381 2002 181 163
BtsI GCAGTG_nn'
Cuts at: 1566 1970 1566
Size: 404 4323
ClaI AT'CG_AT
Cuts at: 905 905
Size: 4727
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 522 557 1635 1670 522
Size: 35 1078 35 3579
Fragments arranged by size:
3579 1078 35 35
DraI TTT'AAA
Cuts at: 612 1726 612
Size: 1114 3613
DraIII CAC_nnn'GTG
Cuts at: 250 250
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3777 2433
Size: 1344 3383
EaeI y'GGCC_r
Cuts at: 129 726 822 892 914 2312 2486 2877 2904
Size: 597 96 70 22 1398 174 391 27
Cuts at: 2904 4249 129
Size: 1345 607
Fragments arranged by size:
1398 1345 607 597 391 174 96 70
27 22
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 234 1585 1692 2750 2960 234
Size: 1351 107 1058 210 2001
Fragments arranged by size:
2001 1351 1058 210 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1108 2935 3677 3823 4537 1108
Size: 1827 742 146 714 1298
Fragments arranged by size:
1827 1298 742 714 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 775 824 871 966 1225 1611 2552 2984
Size: 588 49 47 95 259 386 941 432
Cuts at: 2984 3337 3999 187
Size: 353 662 915
Fragments arranged by size:
941 915 662 588 432 386 353 259
95 49 47
EcoNI CCTnn'n_nnAGG
Cuts at: 328 1387 328
Size: 1059 3668
EcoO109I rG'GnC_Cy
Cuts at: 185 186 196 197 521 1237 1245 1261 1285
Size: 1 10 1 324 716 8 16 24
Cuts at: 1285 1393 1458 1498 1866 185
Size: 108 65 40 368 3046
Fragments arranged by size:
3046 716 368 324 108 65 40 24
16 10 8 1 1
EcoRI G'AATT_C
Cuts at: 1681 1731 1681
Size: 50 4677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 2161 2409 3639 4410 2161
Size: 248 1230 771 2478
Fragments arranged by size:
2478 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 346 378 997 1029 2569 2599 2601
Size: 32 40 32 619 32 1540 30 2
Cuts at: 2601 2631 274
Size: 30 2370
Fragments arranged by size:
2370 1540 619 40 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1201 1766 1201
Size: 565 4162
HindIII A'AGCT_T
Cuts at: 812 812
Size: 4727
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MfeI C'AATT_G
Cuts at: 1134 1134
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3486 3670 3486
Size: 184 4543
MscI TGG'CCA
Cuts at: 824 916 2488 824
Size: 92 1572 3063
Fragments arranged by size:
3063 1572 92
MslI CAynn'nnrTG
Cuts at: 265 512 1180 1420 1423 2716 2843 265
Size: 247 668 240 3 1293 127 2149
Fragments arranged by size:
2149 1293 668 247 240 127 3
MspA1I CmG'CkG
Cuts at: 1838 2152 2512 3296 3541 1838
Size: 314 360 784 245 3024
Fragments arranged by size:
3024 784 360 314 245
NaeI GCC'GGC
Cuts at: 643 1432 2908 643
Size: 789 1476 2462
Fragments arranged by size:
2462 1476 789
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NcoI C'CATG_G
Cuts at: 107 1620 107
Size: 1513 3214
NgoMIV G'CCGG_C
Cuts at: 641 1430 2906 641
Size: 789 1476 2462
Fragments arranged by size:
2462 1476 789
NspI r_CATG'y
Cuts at: 1780 2280 3883 1780
Size: 500 1603 2624
Fragments arranged by size:
2624 1603 500
PasI CC'CwG_GG
Cuts at: 372 412 1300 372
Size: 40 888 3799
Fragments arranged by size:
3799 888 40
PciI A'CATG_T
Cuts at: 3879 3879
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 816 816
Size: 4727
PfoI T'CCnGG_A
Cuts at: 323 1212 1583 323
Size: 889 371 3467
Fragments arranged by size:
3467 889 371
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 160 192 3173 3205 160
Size: 32 2981 32 1682
Fragments arranged by size:
2981 1682 32 32
PpuMI rG'GwC_Cy
Cuts at: 1237 1245 1261 1393 1458 1237
Size: 8 16 132 65 4506
Fragments arranged by size:
4506 132 65 16 8
PspOMI G'GGCC_C
Cuts at: 185 196 185
Size: 11 4716
PstI C_TGCA'G
Cuts at: 1774 2459 1774
Size: 685 4042
PvuI CG_AT'CG
Cuts at: 104 909 104
Size: 805 3922
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1199 1764 1199
Size: 565 4162
SanDI GG'GwC_CC
Cuts at: 1393 1393
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 2750 2960 2750
Size: 210 4517
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SexAI A'CCwGG_T
Cuts at: 828 828
Size: 4727
SfcI C'TryA_G
Cuts at: 33 787 1770 2455 3423 3614 33
Size: 754 983 685 968 191 1146
Fragments arranged by size:
1146 983 968 754 685 191
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SgrAI Cr'CCGG_yG
Cuts at: 641 641
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 442 1882 2797 3511 3773 442
Size: 1440 915 714 262 1396
Fragments arranged by size:
1440 1396 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StuI AGG'CCT
Cuts at: 1552 1552
Size: 4727
StyI C'CwwG_G
Cuts at: 107 1505 1620 1861 2215 107
Size: 1398 115 241 354 2619
Fragments arranged by size:
2619 1398 354 241 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 571 1084 1143 1192 2310 2443 2902 3111 571
Size: 513 59 49 1118 133 459 209 2187
Fragments arranged by size:
2187 1118 513 459 209 133 59 49
TatI w'GTAC_w
Cuts at: 275 4242 275
Size: 3967 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 467 499 667 699 4244 4276 467
Size: 32 168 32 3545 32 918
Fragments arranged by size:
3545 918 168 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1591 2524 1591
Size: 933 3794
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 486 817 1422 2222 486
Size: 331 605 800 2991
Fragments arranged by size:
2991 800 605 331
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
XmnI GAAnn'nnTTC
Cuts at: 175 1514 1552 175
Size: 1339 38 3350
Fragments arranged by size:
3350 1339 38
ZraI GAC'GTC
Cuts at: 453 453
Size: 4727
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BbvCI BcgI BfrBI
BglI BmtI Bpu10I BsiWI BsmI BsmBI BspEI BssHII
BstZ17I EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SfiI SnaBI SrfI SspI SwaI XhoI